FastQCFastQC Report
Wed 9 Apr 2025
RG.AVIR.1.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRG.AVIR.1.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39999009
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGCATATCTGGGGG2113420.5283680903194377TruSeq Adapter, Index 18 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGCATATCTAGGGG578930.14473608583652658TruSeq Adapter, Index 18 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGCATATCTGGTGG465000.1162528801651061TruSeq Adapter, Index 18 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)