FastQCFastQC Report
Wed 9 Apr 2025
RG.MOCK.1.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRG.MOCK.1.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41477375
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCTCCTATCTGGGGG1651800.39824120981619493TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCTCCTATCTAGGGG935590.22556634791859415TruSeq Adapter, Index 8 (97% over 36bp)
CTTCGGGCTTGTAAGCGATGAAACTGATGCACTGCACTTGACGAACATTG680530.16407258173883954No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTCTCTCCTATCTGGGGG513870.12389163971924452TruSeq Adapter, Index 3 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)