FastQCFastQC Report
Wed 9 Apr 2025
RG.MOCK.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRG.MOCK.2.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41068071
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGATATATCTAGTAT1145580.27894662985266583TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGATATATCTAGTGT622370.15154595403324397TruSeq Adapter, Index 12 (97% over 37bp)
CTTCGGGCTTGTAAGCGATGAAACTGATGCACTGCACTTGACGAACATTG590490.14378323247761016No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGATATATCTGGTAT525690.12800455127293414TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGATATATCTGGGGG504850.12293004947809699TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGATATATCTGGTGT502730.12241383336461066TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)