FastQCFastQC Report
Wed 9 Apr 2025
RG.MOCK.3.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRG.MOCK.3.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39578271
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTCGACATCTGGGGG4433391.1201575733310836TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTCGACATCTAGGGG1693960.4280025269421194TruSeq Adapter, Index 14 (97% over 38bp)
CTTCGGGCTTGTAAGCGATGAAACTGATGCACTGCACTTGACGAACATTG694120.17537906089935057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)