FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_112.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_112.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6498431
Total Bases974.7 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCC447669968.88892103340022No Hit
ACAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT112602617.32765955351376No Hit
ATCACGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT1544582.3768506582588937No Hit
CGATGTCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT786981.211030785738896No Hit
TTAGGCCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT641560.9872536924682281No Hit
GATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCAA369350.5683679645132802No Hit
TGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCA234320.3605793459990573No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTATGCATCTGGGGG176400.27145013927207967TruSeq Adapter, Index 15 (97% over 37bp)
GTGATTTTTGGCGCTATCCATCCTGAGTTTCATTGGCTCTTCTTACTACA166500.25621569268027927No Hit
AGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCT133960.2061420672159172No Hit
CAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTC132900.2045109042475022No Hit
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTCGTAAGAAGAGCC109480.1684714356434653No Hit
GTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTC104750.1611927556051607No Hit
GNGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCC103160.1587460111525382No Hit
ATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCAAT102340.1574841681015002No Hit
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTCAGAAGAGCC95730.14731248204374256No Hit
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCCTTGTAGTAAGAAGAGCC95370.14675850216767708No Hit
GCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCT94370.14521966917860632No Hit
TGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCC71080.10938024886314866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)