FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_112.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_112.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6498431
Total Bases974.7 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT329361850.683280317972134No Hit
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCC150252423.121334980705342No Hit
ATCACGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT4556897.012292659566594No Hit
CGATGTCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT2837474.366392441498571No Hit
TTAGGCCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT2532923.897740854677075No Hit
CAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTC421190.6481410666667078No Hit
AGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCT370340.5698914091724602No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG297240.4574027176713887No Hit
GTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTC270700.41656209014145107No Hit
GCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCT245950.3784759736619501No Hit
TAGGCCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTC211610.32563244881726067No Hit
GAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCTTCTTC192410.29608685542710234No Hit
TGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCC179800.27668217143492024No Hit
ACAGTGCTTTGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT140360.21599059834596998No Hit
AAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTC138010.21237434082165374No Hit
GATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCAA128620.1979246990542794No Hit
CTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCTT116490.17925865489685125No Hit
CACGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCT113370.17445749597095053No Hit
CGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCTTCTT111320.1713028883433555No Hit
AGGCCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCT108260.166594059396799No Hit
TGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCA103660.15951542764707358No Hit
TCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCTTCT68920.10605636960675585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)