FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_113.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_113.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8018898
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA167011320.827213415110158No Hit
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG128360616.007261845704985No Hit
TAGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA6014247.500082929100732No Hit
GATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA5695487.102571949412501No Hit
ATCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA4900916.1117001363529No Hit
ACAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA4547145.670529791001208No Hit
GCCAATGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA3804774.744754204380701No Hit
CGATGTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA2546143.175174444169261No Hit
ACTTGAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA2087662.6034250591540133No Hit
TTAGGCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1895432.3637038406025366No Hit
TGACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1430491.7838984858019145No Hit
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCTGTATCTGGG1156221.4418689450844742TruSeq Adapter, Index 20 (97% over 38bp)
GGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGA600100.7483571932203152No Hit
AAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATT576530.7189641269910155No Hit
AGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTT552490.6889849453129345No Hit
GAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGAT514110.6411230071762978No Hit
GAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTG431210.5377422184444796No Hit
AGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT394850.49239932968345523No Hit
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCTGTATCTAGG340230.4242852322102114TruSeq Adapter, Index 20 (97% over 38bp)
GGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG315890.39393193428822765No Hit
CAGATCGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA234550.29249655002470415No Hit
CGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG233540.2912370253368979No Hit
GATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT197080.24576943116123937No Hit
TAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTT171910.21438107829779102No Hit
AGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTGT165600.20651216663436794No Hit
GAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT163500.20389335292704808No Hit
GTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGG162230.20230959416119274No Hit
ATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG158270.19737125974167521No Hit
TCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG143530.17898968162458234No Hit
AGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG140790.17557275326360305No Hit
CGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG127130.1585379936245604No Hit
TAGCTTGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA120800.15064414087821043No Hit
GGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTGTG120630.1504321416733322No Hit
ACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG120360.1500954370538196No Hit
AGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTG118480.14775097525869516No Hit
ATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT117960.1471025071025969No Hit
TGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTG116610.1454189840050341No Hit
CAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT112940.14084229528795603No Hit
ATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCT112190.13990700467819892No Hit
GGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGT110690.13803642345868472No Hit
TGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG108700.13555478570746254No Hit
TCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT107380.13390867423429004No Hit
AGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT99890.12456823867818245No Hit
CAGATCGGAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT99210.12372024185866935No Hit
CACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT97680.12181224901476487No Hit
AGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT90980.1134569862342681No Hit
ATCACGGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA87220.10876806264401917No Hit
CAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG82690.10311890736108627No Hit
CAGATCGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT81070.10109867964401094No Hit
AATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTC80410.10027562390742467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)