FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_113.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_113.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8018898
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG531723566.30879953829067No Hit
CAGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA4622495.7644953209281375No Hit
ATCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1813032.26094657894389No Hit
ACAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1019491.271359231655023No Hit
GCCAATGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA900851.1234087277329128No Hit
TAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTT842091.0501318260938097No Hit
GAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT800170.9978553162791196No Hit
GATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA788840.9837261928010557No Hit
TAGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA751330.9369491917717372No Hit
GTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGG657720.8202124531325875No Hit
AGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTG558630.6966418577714792No Hit
TGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTG512440.6390404267519053No Hit
ATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCT475100.5924754249274651No Hit
CGATGTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA472020.5886344981567293No Hit
GGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGT466800.5821248755128198No Hit
AATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTC374360.46684719022489124No Hit
ACTTGAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA364360.4543766487614632No Hit
TTAGGCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA300690.37497671126381704No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTGACTGTGTAGAT288780.3601242963808743Illumina Single End Sequencing Primer (100% over 33bp)
TTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGT233920.2917109059125082No Hit
GGATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT226120.2819838835710343No Hit
AAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATT212260.2646997131027231No Hit
AGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTT210150.26206842885393977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204920.25554633566856694No Hit
TGACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA198730.247827070502705No Hit
GAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGAT186390.23243842233683482No Hit
GCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGTT167410.20876933463924843No Hit
GGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGA164080.20461664433192692No Hit
GAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTG163510.2039058234685115No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTGACTGTGTCGAT149070.18589836159532144Illumina Single End Sequencing Primer (100% over 33bp)
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTNTTTTCAAG138380.1725673527709169No Hit
AGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT136730.1705097134294513No Hit
GGAATGTTAGGCCAACTCCTCCATCTATCTCAGCTTGAATTGTTTTCAAG134990.1683398392148148No Hit
GGAATGTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT107100.13355949907331405No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTTACTGTGTCGAT105670.13177621164404385Illumina Single End Sequencing Primer (100% over 33bp)
GGAATGCTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG102310.12758610971233203No Hit
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCNTGAATTGTTTTCAAG94390.11770944087329704No Hit
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCNAG93980.1171981486732965No Hit
GGAATGTTAGGCCAACTCCTCCAGCTATCTCNGCTTGAATTGTTTTCAAG93210.11623791698061253No Hit
GGAATGTTAGGCCAACTCNTCCAGCTATCTCAGCTTGAATTGTTTTCAAG92810.11573909532207542No Hit
GGNATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG91040.11353180948304867No Hit
GGAAGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT80230.10005115416108298No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)