FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_114.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_114.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7675105
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG130695017.028431532858505No Hit
ATCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA120588915.711693846533695No Hit
TAGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA92374212.035561728471468No Hit
TGACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA6552218.53696464087462No Hit
GATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA5145836.704572771317135No Hit
ACAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA4154285.412668621471628No Hit
CAGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA3103504.043592888957219No Hit
GCCAATGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA3087604.022876559994945No Hit
ACTTGAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA2513723.275160404971658No Hit
CGATGTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1425001.8566521239774572No Hit
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTCGAATCTGGG571390.7444718997329678TruSeq Adapter, Index 9 (97% over 36bp)
TTAGGCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA548740.7149609028150103No Hit
GGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGA456180.5943632041516044No Hit
GGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG445350.5802526480093758No Hit
AAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATT436250.5683961326913443No Hit
AGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTT428430.5582073470004645No Hit
GAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGAT391880.5105858486626568No Hit
GAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTG330720.43089964241531553No Hit
ACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG299140.3897536255204326No Hit
CGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG277030.3609462020389298No Hit
ATCACGGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA254280.33130491374385107No Hit
TCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT253210.329910796008654No Hit
CACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT229510.2990317396309236No Hit
TAGCTTGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA221910.2891295949697105No Hit
TGACCAGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA172590.2248698877735223No Hit
GTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGG172480.22472656725868898No Hit
TAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTT166600.21706543428396094No Hit
AGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT163880.2135215088262636No Hit
GAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT163500.21302640159320296No Hit
ATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT150850.196544542387368No Hit
AGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG135910.17707901064545697No Hit
GACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT127750.166447234272365No Hit
AGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTGT124620.16236911416847066No Hit
TGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTG120230.15664932271284887No Hit
ATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCT118950.1549815930856972No Hit
TGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG114320.14894910232498448No Hit
AGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTG111040.1446755451554083No Hit
AGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG110030.14335960224648392No Hit
CAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT98060.12776372440507328No Hit
ATACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT96290.12545756703002758No Hit
GGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGT93780.12218725346428486No Hit
GGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTGTG92140.12005047487949677No Hit
AGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT87410.11388769274166281No Hit
CTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG84130.10961413557208663No Hit
AATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTC83420.10868906679452593No Hit
GCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT83120.10829819266316226No Hit
CAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG80900.10540572409107106No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)