FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_114.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_114.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7675105
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG510830766.55683537879938No Hit
ATCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA4014075.229987081609958No Hit
TGACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA2836433.6956237080795638No Hit
TAGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1374301.7905943957769959No Hit
GATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1235351.6095545272670537No Hit
ACAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA788991.0279859363487536No Hit
GAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT771331.0049764791491451No Hit
TAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTT746470.9725860427968086No Hit
GTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGG658370.8577993395530094No Hit
GCCAATGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA573880.7477161550232864No Hit
AGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTG514890.6708572716594757No Hit
TGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTG476470.6207993245695009No Hit
ATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCT474770.6185843711584401No Hit
GGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGT396620.5167616599382028No Hit
ACTTGAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA390960.5093871679931414No Hit
AATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTC357990.46643010095627363No Hit
CAGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA303820.39585126196970594No Hit
CGATGTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA234690.3057808329658031No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG219660.286198038984483No Hit
TTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGT218890.2851947953806495No Hit
GGATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT214480.2794489456496035No Hit
GACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT151630.19756081512891355No Hit
GCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGTT137700.1794112262959269No Hit
AAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATT137270.17885097337430564No Hit
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTNTTTTCAAG136240.177508972189957No Hit
AGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTT135680.17677934047807814No Hit
GGAATGTTAGGCCAACTCCTCCATCTATCTCAGCTTGAATTGTTTTCAAG127590.16623876806897106No Hit
GAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGAT123890.16141798711548572No Hit
GGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG118180.15397834948186379No Hit
CACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT109500.14266905794774143No Hit
GGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGA108460.14131402762568068No Hit
GAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTG99180.12922298782883102No Hit
GGAATGTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT98420.1282327733627097No Hit
ACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG97500.1270340926931944No Hit
TCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT96900.12625234443046707No Hit
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCNAG92940.12109280589646657No Hit
GGAATGTTAGGCCAACTCNTCCAGCTATCTCAGCTTGAATTGTTTTCAAG91730.11951628023329974No Hit
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCNTGAATTGTTTTCAAG90790.11829154128836022No Hit
GGAATGTTAGGCCAACTCCTCCAGCTATCTCNGCTTGAATTGTTTTCAAG90220.11754888043876924No Hit
CGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG89980.1172361811336783No Hit
GGAATGCTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG89110.11610264615272364No Hit
GGNATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG88790.11568571374593574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)