FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_112.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_112.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6474721
Total Bases968.2 Mbp
Sequences flagged as poor quality0
Sequence length5-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCC447608169.13164289241189No Hit
ACAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT112588317.38890370720221No Hit
ATCACGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT1544422.3853074132460685No Hit
CGATGTCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT786931.2153882769620497No Hit
TTAGGCCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT641500.9907762820977152No Hit
GATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCAA369320.5704029563590461No Hit
TGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCA234310.3618843190308895No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTATGCATCTGGGGG176370.2723978376828901TruSeq Adapter, Index 15 (97% over 37bp)
GTGATTTTTGGCGCTATCCATCCTGAGTTTCATTGGCTCTTCTTACTACA166500.25715393759823785No Hit
AGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCT133960.20689694582978943No Hit
CAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTC132890.2052443649695485No Hit
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTCGTAAGAAGAGCC109480.1690883668964269No Hit
GTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTC104720.1617366987704953No Hit
ATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCAAT102330.15804542002659264No Hit
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTCAGAAGAGCC95710.14782104124641046No Hit
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCCTTGTAGTAAGAAGAGCC95370.1472959220945582No Hit
GCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCT94370.14575145400087508No Hit
TGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCC71070.10976534741805864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)