FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_112.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_112.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6474309
Total Bases970.2 Mbp
Sequences flagged as poor quality0
Sequence length5-150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT328748150.777326198054496No Hit
GTGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCC149971123.16403186811133No Hit
ATCACGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT4547757.024301744016234No Hit
CGATGTCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT2831934.37410386189476No Hit
TTAGGCCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT2528393.905266183618978No Hit
CAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTC420270.6491349115403667No Hit
AGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCT369640.5709335158392965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG297150.45896789912251645No Hit
GTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTC270320.4175271832098221No Hit
GCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCT245480.37916015438867684No Hit
TAGGCCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTC211160.32615063630728774No Hit
GAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCTTCTTC192010.29657219017504416No Hit
TGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCC179430.2771415451440455No Hit
ACAGTGCTTTGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTT140100.2163937495105655No Hit
AAGTGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTC137800.21284124684194097No Hit
GATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCAA128350.19824509457302703No Hit
CTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCTT116320.17966396104974292No Hit
CACGCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCT113210.17486035961521146No Hit
CGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCTTCTT111140.17166310721344935No Hit
AGGCCTTCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCT107920.16668960347737496No Hit
TGATTTTAGGCGTTTTGCCTCGGCCTTTTTCATTGTAGTAAGAAGAGCCA103490.15984717442432853No Hit
TCGAACCCGAGTTTTGTTCGTCTATAAATAGCACCTTCTCTTCTCCTTCT68860.10635884076586398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)