FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_113.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_113.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7559521
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length5-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA163479521.62564268291602No Hit
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG125689116.62659578563245No Hit
TAGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA5889727.791128564891876No Hit
GATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA5579877.381248097597719No Hit
ATCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA4798446.347545036252958No Hit
ACAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA4454005.891907701559398No Hit
GCCAATGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA3726814.929955218062097No Hit
CGATGTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA2493513.2985026432230296No Hit
TTAGGCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1855512.45453382562202No Hit
TGACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1401801.8543502954750706No Hit
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCTGTATCTGGG1130571.495557721183657TruSeq Adapter, Index 20 (97% over 38bp)
GGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGA587100.7766365091121514No Hit
AAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATT564910.7472827974153389No Hit
AGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTT541130.7158257778502103No Hit
GAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGAT503300.6657829246059374No Hit
GAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTG422330.5586729635382983No Hit
AGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT386400.511143497054906No Hit
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCTGTATCTAGG331920.4390754387744938TruSeq Adapter, Index 20 (97% over 38bp)
GGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG309840.4098672389427849No Hit
CAGATCGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA233870.30937145356167406No Hit
CGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG228400.30213554536061216No Hit
GATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT192800.25504261447253074No Hit
TAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTT168440.2228183505277649No Hit
AGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTGT161880.21414055202703983No Hit
GAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT160370.21214307096970825No Hit
GTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGG158780.2100397631013923No Hit
ATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG154870.20486747771452715No Hit
TCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG140640.18604353371066765No Hit
AGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG138120.1827099891646574No Hit
CGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG124580.1647988014055388No Hit
TAGCTTGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA120220.15903124020688614No Hit
GGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTGTG118000.15609454620206756No Hit
ACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG117830.15586966422872559No Hit
AGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTG115890.15330336406235262No Hit
ATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT115710.1530652537376376No Hit
TGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTG114210.15108100103167912No Hit
CAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT110570.14626588113188654No Hit
ATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCT110060.14559123521186063No Hit
GGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGT108390.1433821005325602No Hit
TGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG106510.14089517047442557No Hit
TCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT105210.1391754847959282No Hit
AGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT98040.12969075686144665No Hit
CAGATCGGAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT97060.1283943784268871No Hit
CACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT95480.12630429890994416No Hit
AGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT89130.11790429578805324No Hit
ATCACGGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA86960.11503374354009996No Hit
CAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG80920.10704381931077378No Hit
CAGATCGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT79600.10529767692953032No Hit
AATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTC78700.10410712530595523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)