FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_113.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_113.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7675870
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length5-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG519470767.67580743290337No Hit
CAGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA4516245.883684846147733No Hit
ATCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1772252.3088588003705115No Hit
ACAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA995801.297312226496801No Hit
GCCAATGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA880581.1472054633546427No Hit
TAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTT822561.0716179403767911No Hit
GAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT780771.0171746004036024No Hit
GATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA770351.0035995919680767No Hit
TAGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA734530.9569338719910577No Hit
GTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGG642890.8375467536578916No Hit
AGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTG545780.7110334072880339No Hit
TGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTG500570.6521345463120142No Hit
ATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCT464120.6046480724660527No Hit
CGATGTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA461200.6008439434227No Hit
GGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGT456340.5945124135765718No Hit
AATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTC365670.4763889956447933No Hit
TTAGGCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA293570.3824582750880356No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTGACTGTGTAGAT282190.36763259409031157Illumina Single End Sequencing Primer (100% over 33bp)
TTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGT228290.29741254085856067No Hit
GGATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT220690.28751138307449187No Hit
AAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATT207240.26998893936452806No Hit
AGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTT205830.2681520140387995No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200390.2610648695196766No Hit
TGACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA193960.2526879689207868No Hit
GAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGAT182740.23807073335009582No Hit
GCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGTT164040.21370867406561078No Hit
GGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGA160540.20914893034926335No Hit
GAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTG159850.2082500094451834No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTGACTGTGTCGAT146560.19093601116225262Illumina Single End Sequencing Primer (100% over 33bp)
AGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT133380.17376531911040702No Hit
GGAATGTTAGGCCAACTCCTCCATCTATCTCAGCTTGAATTGTTTTCAAG132030.17200656081981588No Hit
GGAATGTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT104720.13642753199311608No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTTACTGTGTCGAT103740.1351508037525388Illumina Single End Sequencing Primer (100% over 33bp)
GGAATGCTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG100090.13039564244834786No Hit
GGAAGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT78350.10207312005023535No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTTACTGTGTAGAT77550.1010308929150702Illumina Single End Sequencing Primer (100% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)