FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_114.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_114.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7173156
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length5-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG127900817.830477965347473No Hit
ATCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA117983816.44796237527805No Hit
TAGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA90372912.59876405866539No Hit
TGACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA6409948.936010871644225No Hit
GATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA5035857.0204105417475935No Hit
ACAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA4064725.6665713111495135No Hit
CAGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA3033724.229268121312293No Hit
GCCAATGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA3021724.212539083215254No Hit
CGATGTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1394631.944234866772729No Hit
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTCGAATCTGGG558130.7780815027583395TruSeq Adapter, Index 9 (97% over 36bp)
TTAGGCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA536490.7479134707233469No Hit
GGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGA445460.6210097758922294No Hit
GGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG435280.6068179752399083No Hit
AAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATT427610.5961253317228846No Hit
AGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTT419270.5844986502454429No Hit
GAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGAT383540.5346879393115109No Hit
GAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTG323330.4507499906596204No Hit
ACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG292460.407714540154989No Hit
CGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG271140.377992615802584No Hit
ATCACGGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA253310.35313605336340104No Hit
TCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT247620.34520370113238863No Hit
CACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT224430.3128748350098618No Hit
TAGCTTGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA220830.30785612358075026No Hit
TGACCAGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA171840.2395598255495907No Hit
GTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGG168620.23507086699355206No Hit
TAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTT163060.22731974600859092No Hit
AGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT160130.22323507253989736No Hit
GAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT159920.2229423143731992No Hit
ATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT147280.20532106091098534No Hit
AGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG132600.18485587097227496No Hit
GACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT124950.17419110918541295No Hit
AGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTGT122010.17009249485163852No Hit
TGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTG117820.16425127238275594No Hit
ATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCT116380.16224378781111132No Hit
TGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG111860.15594251679456017No Hit
AGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTG108400.15111897747658073No Hit
AGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG107690.1501291760558393No Hit
CAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT96000.13383230477630767No Hit
ATACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT94360.13154600290304574No Hit
GGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGT91940.1281723135534763No Hit
GGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTGTG90190.12573266216432488No Hit
AGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT85440.11911075125091383No Hit
CTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG82160.11453814750438998No Hit
AATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTC81620.11378534079002325No Hit
GCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT81320.11336711483759729No Hit
CAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG79150.1103419471150495No Hit
CAGATCGGAAGGGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA73410.10233989055863277No Hit
CAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG72970.1017264924950747No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)