FastQCFastQC Report
Tue 29 Jul 2025
AC_HS_114.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAC_HS_114.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7335480
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length5-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG498847168.00469771575956No Hit
ATCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA3919505.3432086243844985No Hit
TGACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA2770583.776958017743897No Hit
TAGCTTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1341711.8290691270373582No Hit
GATCAGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA1206721.6450457229792734No Hit
ACAGTGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA770811.0507969485296123No Hit
GAATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT752861.0263268388708033No Hit
TAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTT728720.9934182902822991No Hit
GTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGG642790.8762753085006024No Hit
GCCAATGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA559880.7632493033857362No Hit
AGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTG502910.6855856740117893No Hit
TGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTG465140.6340962009302732No Hit
ATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCT464060.6326239046388239No Hit
GGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGT387230.5278863823498939No Hit
AATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTC349590.4765741301182745No Hit
CAGATCGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA296380.40403627301826195No Hit
CGATGTGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTA229380.31269937345613374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214790.29280974114850017No Hit
TTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGT213430.29095573841111966No Hit
GGATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT209780.285979922240944No Hit
GACCAGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT147870.20158190057092376No Hit
GCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTCTGGTTGTT134820.18379165371591225No Hit
AAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATT133920.18256474013970456No Hit
AGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTT132330.18039719282173763No Hit
GGAATGTTAGGCCAACTCCTCCATCTATCTCAGCTTGAATTGTTTTCAAG124680.16996842742397225No Hit
GAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGAT120950.1648835522692448No Hit
GGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGG115050.15684045215854994No Hit
CACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATT106840.145648273868922No Hit
GGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGA105840.14428503656202457No Hit
GAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGGGATTTG96740.1318795770692579No Hit
GGAATGTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT95990.13085714908908483No Hit
ACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTG94800.1292348966938769No Hit
TCACGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTAT94640.1290167787247733No Hit
CGGGAAGAGGTCATGGAAGCTTTGAGAGATGAGGATGTCTCTGTTATTGG88000.11996488300697432No Hit
GGAATGCTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAG86690.11817904213493868No Hit
GATGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGTC73700.10047058951834098No Hit
GGAAGTTAGGCCAACTCCTCCAGCTATCTCAGCTTGAATTGTTTTCAAGT73520.10022520680309946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)