FastQCFastQC Report
Mon 13 Oct 2025
deltaA4.1.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamedeltaA4.1.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9198432
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCTCCATCTGGGGG166920.18146571067764594TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCTCCATCTAGGGG96400.10480047034103204TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)