FastQCFastQC Report
Fri 24 Oct 2025
nt.24h.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenament.24h.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31582907
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length20-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCACTTGGTTTTCAGCAATGGCTTCTTCAGTAATGTCCTCAGCAGCTG375100.11876677469873183No Hit
GCAAAACCTTGACATTACCTCCATTGCTAGCAACGGTGGAAGAGTCAGTT354770.11232974849338599No Hit
CTTGGTTTTCAGCAATGGCTTCTTCAGTAATGTCCTCAGCAGCTGTTGCC352380.11157301004622532No Hit
GTCAGTTGCATGCAGGTGTGGCCACCAATTAACATGAAGAAGTACGAGAC347140.10991388474784795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)