##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.1.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24147199
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.26722055837615	37.0	35.0	39.0	33.0	39.0
2	35.67252268058088	36.0	34.0	39.0	33.0	39.0
3	35.94915517944752	37.0	35.0	39.0	33.0	39.0
4	35.95366630307722	37.0	35.0	39.0	33.0	39.0
5	35.7459007564397	36.0	35.0	38.0	33.0	39.0
6	35.60885666283696	36.0	35.0	38.0	33.0	39.0
7	35.46099131414786	35.0	34.0	37.0	33.0	39.0
8	35.74914196052304	36.0	35.0	38.0	33.0	39.0
9	35.720769104524294	36.0	35.0	38.0	33.0	39.0
10-14	35.73988911923077	36.0	35.0	38.0	33.0	39.0
15-19	35.73896383592979	36.0	35.0	38.0	33.0	39.0
20-24	35.72409761479996	36.0	35.0	38.0	33.0	39.0
25-29	35.71249785948258	36.0	35.0	38.0	33.0	39.0
30-34	35.70651744742734	36.0	35.0	38.0	33.0	39.0
35-39	35.69346459603865	36.0	35.0	38.0	33.0	39.0
40-44	35.67683719341527	36.0	35.0	38.0	33.0	39.0
45-49	35.66698393465843	36.0	35.0	38.0	33.0	39.0
50-54	35.644799382321736	36.0	35.0	38.0	33.0	39.0
55-59	35.62625252725999	36.0	35.0	38.0	33.0	39.0
60-64	35.61439096932112	36.0	35.0	38.0	33.0	39.0
65-69	35.59537792354301	36.0	35.0	38.0	33.0	39.0
70-74	35.57597105154929	36.0	35.0	38.0	33.0	39.0
75-79	35.557457442579576	36.0	35.0	38.0	33.0	39.0
80-84	35.53492887518755	36.0	34.0	38.0	33.0	39.0
85-89	35.51564552062539	36.0	34.0	38.0	33.0	39.0
90-94	35.496803633415205	36.0	34.0	38.0	33.0	39.0
95-99	35.470731855897654	36.0	34.0	38.0	33.0	39.0
100-104	35.435735382807756	36.0	34.0	38.0	32.0	39.0
105-109	35.40812649947516	36.0	34.0	38.0	32.0	39.0
110-114	35.38044905332499	36.0	34.0	38.0	32.0	39.0
115-119	35.349298309919924	36.0	34.0	38.0	32.0	39.0
120-124	35.31850401365392	36.0	34.0	38.0	32.0	39.0
125-129	35.283584004919156	36.0	34.0	38.0	32.0	39.0
130-134	35.248669454374394	36.0	34.0	38.0	32.0	39.0
135-139	35.20767537468838	36.0	34.0	38.0	32.0	39.0
140-144	35.17201859312958	36.0	34.0	38.0	32.0	39.0
145-149	35.1309657074512	36.0	34.0	38.0	32.0	39.0
150	35.117532182511106	36.0	34.0	38.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	10.0
22	218.0
23	2109.0
24	13207.0
25	46379.0
26	109927.0
27	193312.0
28	281144.0
29	364846.0
30	459168.0
31	587463.0
32	783152.0
33	1111170.0
34	1755232.0
35	3372159.0
36	1.1870563E7
37	3197109.0
38	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.38682185236134	10.678230153553894	14.001611038516874	38.933336955567896
2	22.471743577351628	12.82435847023864	36.883944761217315	27.819953191192425
3	21.068445790413186	17.69097726077029	28.216588380053516	33.023988568763016
4	26.245216725675675	25.759205160424237	22.08758528227872	25.907992831621375
5	25.68612141274941	31.399052576896754	24.254111783800546	18.660714226553292
6	19.09211209298847	37.26518465976504	25.466255543197075	18.176447704049412
7	15.852115206023065	25.06769839536095	40.4943932364759	18.585793162140085
8	18.432367671918627	24.636980384458973	31.430775767605162	25.49987617601724
9	19.55363551475096	23.183609244179813	32.72311556815018	24.539639672919048
10-14	20.196708349435283	29.775171597260275	26.837466290681295	23.19065376262315
15-19	20.399709726565312	28.408502245928418	27.593916290755015	23.597871736751255
20-24	20.55755461569657	28.609891635548312	27.264617192202188	23.567936556552922
25-29	20.53931933204081	28.821364251381866	27.145906192311926	23.4934102242654
30-34	20.54893611706342	28.509838171081398	27.171469616944627	23.76975609491056
35-39	20.624217930065846	28.522157069617343	27.177098244176122	23.67652675614068
40-44	20.70826671988702	28.61316339594138	27.042675070274747	23.63589481389685
45-49	20.793299836435928	28.379913920969102	27.02565259149831	23.801133651096663
50-54	20.92616236873929	28.36157394062501	27.087337921092658	23.624925769543044
55-59	20.94671865898366	28.414632143374597	26.99386231216393	23.644786885477814
60-64	20.89670407471798	28.340049179365867	26.965258257103052	23.797988488813097
65-69	21.0040824634103	28.260713583087337	27.045785247196314	23.68941870630605
70-74	21.117819973784215	28.174739011838994	27.034886771511868	23.672554242864923
75-79	21.135115486240995	28.085755001782076	27.018691760009343	23.760437751967586
80-84	21.18505341411944	28.07225576797359	27.013855288771786	23.728835529135182
85-89	21.177867251088152	28.078799132922533	27.052013301054117	23.6913203149352
90-94	21.16925023807372	27.951705788590537	27.004223075273703	23.87482089806204
95-99	21.262131600127802	27.8669790014687	27.121127469829275	23.749761928574223
100-104	21.327651208603193	27.916067031042367	27.004647386257908	23.751634374096533
105-109	21.302388899346525	27.74407752145716	27.10745198870615	23.846081590490165
110-114	21.409150392841227	27.69939404882396	27.115907626404102	23.775547931930713
115-119	21.45319444984536	27.750726824995102	27.07447053682988	23.72160818832966
120-124	21.427477540544235	27.657244516671213	27.056666977869625	23.858610964914924
125-129	21.456735348405125	27.564978438330556	27.137125985874892	23.841160227389427
130-134	21.552037639471152	27.67693385812324	27.005119086086548	23.765909416319065
135-139	21.544301908837163	27.537378177038306	27.10979316591356	23.80852674821097
140-144	21.622885337030308	27.442979044456976	27.21019305394354	23.72394256456917
145-149	21.61397087559707	27.81406578199836	27.003014690001176	23.568948652403396
150	21.483137501949507	27.38209428911936	27.126896301648056	24.007871907283075
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	3.0
10	2.5
11	1.0
12	4.0
13	12.0
14	30.0
15	68.0
16	138.0
17	337.0
18	658.0
19	1170.0
20	2182.0
21	3729.0
22	5921.0
23	9664.0
24	15651.0
25	24552.0
26	36595.5
27	51516.5
28	69812.5
29	96592.5
30	129528.0
31	164592.0
32	199786.5
33	237254.5
34	278035.0
35	325161.0
36	395721.0
37	493304.0
38	611131.5
39	757406.0
40	924439.5
41	1087336.0
42	1256726.5
43	1432709.0
44	1575044.5
45	1662690.0
46	1686731.0
47	1640555.5
48	1544543.5
49	1415178.0
50	1250654.0
51	1069341.0
52	889148.5
53	711702.0
54	549061.5
55	411912.5
56	305203.5
57	229218.5
58	172355.5
59	125812.0
60	88509.0
61	62804.5
62	45318.0
63	30489.0
64	20237.5
65	14645.5
66	12250.5
67	9123.0
68	5026.5
69	2881.0
70	2242.5
71	1382.5
72	664.0
73	302.5
74	158.5
75	97.0
76	50.5
77	36.5
78	23.0
79	10.0
80	7.5
81	6.0
82	3.5
83	2.5
84	1.0
85	1.0
86	0.5
87	0.0
88	0.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.739332168505341E-4
2	1.4080308030757523E-4
3	1.2837927910396566E-4
4	1.532268815111848E-4
5	6.21190060180479E-5
6	1.3666181323970537E-4
7	9.939040962887662E-5
8	7.868407428952732E-5
9	1.1181421083248622E-4
10-14	7.702756746237938E-5
15-19	1.4825736102974096E-4
20-24	0.020100882094026724
25-29	3.9342037144763666E-4
30-34	2.840909208558723E-4
35-39	2.559303047943573E-4
40-44	1.9546780560345735E-4
45-49	3.602902349046778E-4
50-54	4.248940011634476E-4
55-59	3.843095838983229E-4
60-64	3.313013654295887E-4
65-69	2.600715718622271E-4
70-74	2.865756810965943E-4
75-79	1.8801352488129162E-4
80-84	1.8387225781342175E-4
85-89	2.4930427748576554E-4
90-94	2.3439571604143405E-4
95-99	1.4080308030757523E-4
100-104	1.4328784054829714E-4
105-109	1.5239862809761083E-4
110-114	1.6150941564692451E-4
115-119	2.153458875292327E-4
120-124	1.7476147026410807E-4
125-129	1.7724623050482998E-4
130-134	1.5405513492475876E-4
135-139	1.971243124306053E-4
140-144	1.7144845660981218E-4
145-149	1.6399417588764645E-4
150	2.6918235941154087E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4147199E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.25799025265707
#Duplication Level	Percentage of total
1	30.797437137171148
2	10.956466363657752
3	6.993725036634723
4	5.11339815332079
5	3.8985860838734907
6	2.996701059083218
7	2.469001208271131
8	2.1197202109762743
9	1.812710728380324
>10	21.18227217471799
>50	5.45265842177201
>100	5.492401748376888
>500	0.42254535688894107
>1k	0.29237631687536625
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.141267067869859E-6	1.2423801203609579E-5	0.0	0.0	1.6979194978266422E-4	8.282534135739719E-6
2	1.2423801203609579E-5	7.868407428952732E-5	0.0	0.0	0.0021907302789031555	3.3130136542958875E-5
3	1.2423801203609579E-5	9.110787549313691E-5	0.0	4.141267067869859E-6	0.0033171549213637575	8.696660842526704E-5
4	3.3130136542958875E-5	9.110787549313691E-5	0.0	4.141267067869859E-6	0.00501093315212253	1.2009674496822593E-4
5	3.3130136542958875E-5	9.524914256100676E-5	0.0	4.141267067869859E-6	0.006315432278501535	1.325205461718355E-4
6	6.21190060180479E-5	9.524914256100676E-5	0.0	4.141267067869859E-6	0.007425291852690658	1.408030803075752E-4
7	9.524914256100676E-5	1.4494434737544507E-4	0.0	4.141267067869859E-6	0.00850202129033682	1.4908561444331494E-4
8	9.939040962887663E-5	1.4908561444331494E-4	0.0	4.141267067869859E-6	0.010763153109393764	1.532268815111848E-4
9	1.1595547790035606E-4	1.4908561444331494E-4	0.0	4.141267067869859E-6	0.014705639358005871	1.6150941564692451E-4
10-11	1.7807448391840395E-4	1.739332168505341E-4	0.0	4.141267067869859E-6	0.01977040898201071	2.2776968873284225E-4
12-13	2.2776968873284228E-4	1.7807448391840395E-4	0.0	4.141267067869859E-6	0.024006925192441575	2.9402996181876E-4
14-15	2.4019348993645185E-4	1.884276515880786E-4	0.0	4.141267067869859E-6	0.02896195123914786	3.3958389956532844E-4
16-17	2.6711172587760596E-4	2.153458875292327E-4	0.0	4.141267067869859E-6	0.033691278230655236	3.437251666331983E-4
18-19	2.857474276830203E-4	2.4019348993645185E-4	0.0	4.141267067869859E-6	0.037782850093710664	3.4579580016713327E-4
20-21	3.54078334302873E-4	3.002418624205648E-4	0.0	4.141267067869859E-6	0.041822656118417706	3.54078334302873E-4
22-23	4.369036756602702E-4	3.1680693069204425E-4	0.0	4.141267067869859E-6	0.046334566588861925	3.9756163851550647E-4
24-25	5.052345822801228E-4	3.3130136542958876E-4	0.0	8.282534135739719E-6	0.05137655924399348	4.058441726512462E-4
26-27	5.694242218321057E-4	3.3544263249745863E-4	0.0	1.6565068271479437E-5	0.05625497184994417	4.4311557626207495E-4
28-29	5.984130913071947E-4	3.3544263249745863E-4	0.0	1.6565068271479437E-5	0.06104227658040173	4.679631786692941E-4
30-31	6.253313272483488E-4	3.3544263249745863E-4	0.0	1.6565068271479437E-5	0.06594553678875964	4.969520481443832E-4
32-33	6.667439979270473E-4	3.4165453309926335E-4	0.0	2.0706335339349298E-5	0.07016755856445296	5.61141687696366E-4
34-35	7.185098362754206E-4	3.437251666331983E-4	0.0	2.4847602407219158E-5	0.07378909661530515	5.963424577732598E-4
36-37	7.433574386826397E-4	3.54078334302873E-4	0.0	2.4847602407219158E-5	0.0773775045296144	6.17048793112609E-4
38-39	7.619931404880541E-4	3.851378373118969E-4	0.0	3.3130136542958875E-5	0.08148357082740734	6.274019607822837E-4
40-41	8.158296123703622E-4	3.892791043797668E-4	0.0	3.3130136542958875E-5	0.08548610544850357	6.315432278501535E-4
42-43	8.365359477097116E-4	3.892791043797668E-4	0.0	3.3130136542958875E-5	0.08898133485378573	6.418963955198282E-4
44-45	8.84160518990215E-4	3.9756163851550647E-4	0.0	3.3130136542958875E-5	0.09349324532422995	6.418963955198282E-4
46-47	9.359263573385882E-4	4.0998543971911607E-4	0.0	3.3130136542958875E-5	0.09768006632984638	6.584614637913076E-4
48-49	9.752683944833519E-4	4.2033860738879077E-4	0.0	3.3130136542958875E-5	0.10162669384552635	6.750265320627871E-4
50-51	9.897628292208963E-4	4.3069177505846535E-4	0.0	3.3130136542958875E-5	0.10599987186919692	6.833090661985268E-4
52-53	0.0010415286675692697	4.327624085924003E-4	0.0	3.3130136542958875E-5	0.10984710897524802	6.853796997324617E-4
54-55	0.0010725881705782934	4.348330421263352E-4	0.0	3.3130136542958875E-5	0.11312078059239915	6.874503332663966E-4
56-57	0.001116071474790927	4.389743091942051E-4	0.0	3.934203714476367E-5	0.1175643601562235	6.998741344700062E-4
58-59	0.0011305659095284717	4.5761001099961946E-4	0.0	5.383647188230817E-5	0.12211561266381248	7.143685692075507E-4
60-61	0.0011678373131393002	4.679631786692941E-4	0.0	5.383647188230817E-5	0.12615541868851954	7.247217368772254E-4
62-63	0.0012196031514876736	4.803869798729037E-4	0.0	5.383647188230817E-5	0.1298701352483988	7.247217368772254E-4
64-65	0.0012444507538948927	4.886695140086434E-4	0.0	5.383647188230817E-5	0.13491419853706427	7.247217368772254E-4
66-67	0.0012775808904378517	5.052345822801228E-4	0.0	5.383647188230817E-5	0.13928944719426878	7.288630039450953E-4
68-69	0.0013417705299898345	5.259409176194721E-4	0.0	5.383647188230817E-5	0.14234570229035676	7.454280722165748E-4
70-71	0.001416313337211492	5.507885200266913E-4	0.0	5.383647188230817E-5	0.14530049634328188	7.557812398862494E-4
72-73	0.0014494434737544507	5.59071054162431E-4	0.0	5.5907105416243096E-5	0.14843336488012543	7.682050410898589E-4
74-75	0.0015115624797724988	5.652829547642358E-4	0.0	5.797773895017803E-5	0.1520176315273668	7.744169416916637E-4
76-77	0.001532268815111848	5.756361224339105E-4	0.0	6.004837248411296E-5	0.15473430272388944	7.826994758274035E-4
78-79	0.0015798933863923515	5.839186565696501E-4	0.0	8.903724195920198E-5	0.1573992080820637	7.930526434970781E-4
80-81	0.0016378711253425293	5.8805992363752E-4	0.0	9.110787549313691E-5	0.16068116223335055	7.95123277031013E-4
82-83	0.0016792837960212278	5.922011907053898E-4	4.141267067869859E-6	9.110787549313691E-5	0.16376847683244752	7.97193910564948E-4
84-85	0.0017496853361750157	5.963424577732598E-4	4.141267067869859E-6	9.110787549313691E-5	0.16626359024083912	8.034058111667527E-4
86-87	0.0018511463793378273	5.963424577732598E-4	4.141267067869859E-6	9.524914256100676E-5	0.1684191197496654	8.034058111667527E-4
88-89	0.0019070534847540704	5.963424577732598E-4	4.141267067869859E-6	9.73197760949417E-5	0.17054358975548262	8.034058111667527E-4
90-91	0.001940183621297029	6.149781595786741E-4	4.141267067869859E-6	9.939040962887663E-5	0.17353358457848467	8.034058111667527E-4
92-93	0.001973313757839988	6.232606937144139E-4	4.141267067869859E-6	9.939040962887663E-5	0.1764593897619347	8.116883453024924E-4
94-95	0.0020623509997991897	6.253313272483488E-4	4.141267067869859E-6	9.939040962887663E-5	0.179600540832914	8.241121465061021E-4
96-97	0.002184518378301351	6.253313272483488E-4	4.141267067869859E-6	9.939040962887663E-5	0.18203974713588933	8.26182780040037E-4
98-99	0.0022611318190569434	6.274019607822837E-4	4.141267067869859E-6	9.939040962887663E-5	0.18422633614772463	8.282534135739719E-4
100-101	0.0023460277939482757	6.294725943162186E-4	4.141267067869859E-6	9.939040962887663E-5	0.18656408140753716	8.282534135739719E-4
102-103	0.0023729460298894294	6.294725943162186E-4	4.141267067869859E-6	1.0146104316281155E-4	0.18856224276778438	8.282534135739719E-4
104-105	0.002422641234703868	6.377551284519583E-4	4.141267067869859E-6	1.0560231023068142E-4	0.19039268281178284	8.282534135739719E-4
106-107	0.0025220316443327444	6.48108296121633E-4	4.141267067869859E-6	1.0974357729855127E-4	0.19214858004855967	8.344653141757767E-4
108-109	0.002613139519825881	6.522495631895028E-4	4.141267067869859E-6	1.1595547790035606E-4	0.1940411390985762	8.448184818454513E-4
110-111	0.002698035494717213	6.626027308591775E-4	4.141267067869859E-6	1.1595547790035606E-4	0.19570385782632593	8.489597489133212E-4
112-113	0.0027560132336673913	6.626027308591775E-4	4.141267067869859E-6	1.1595547790035606E-4	0.19751152090145113	8.489597489133212E-4
114-115	0.0027974259043460903	6.667439979270473E-4	4.141267067869859E-6	1.2009674496822593E-4	0.1996132139383951	8.489597489133212E-4
116-117	0.002861615543898073	6.708852649949173E-4	4.141267067869859E-6	1.2837927910396566E-4	0.20120967239305892	8.489597489133212E-4
118-119	0.002942370251721535	6.874503332663967E-4	4.141267067869859E-6	1.3666181323970534E-4	0.20279991894712096	8.489597489133212E-4
120-121	0.0031059503009023947	6.998741344700063E-4	4.141267067869859E-6	1.408030803075752E-4	0.20445228450720102	8.489597489133212E-4
122-123	0.0032840247848207986	7.06086035071811E-4	4.141267067869859E-6	1.408030803075752E-4	0.20593692875103237	8.510303824472562E-4
124-125	0.0034082627968568943	7.081566686057459E-4	4.141267067869859E-6	1.408030803075752E-4	0.20731804131816695	8.531010159811911E-4
126-127	0.003447604834001658	7.102273021396808E-4	4.141267067869859E-6	1.408030803075752E-4	0.20871778958710696	8.531010159811911E-4
128-129	0.0035076532064857707	7.164392027414856E-4	4.141267067869859E-6	1.408030803075752E-4	0.21008233708597007	8.55171649515126E-4
130-131	0.0036091142496485823	7.205804698093556E-4	4.141267067869859E-6	1.4494434737544507E-4	0.2115048623237834	8.593129165829958E-4
132-133	0.0037064340257435243	7.205804698093556E-4	4.141267067869859E-6	1.532268815111848E-4	0.2127161829411353	8.696660842526704E-4
134-135	0.0039031442114673422	7.205804698093556E-4	4.141267067869859E-6	1.532268815111848E-4	0.21392750355848728	8.717367177866055E-4
136-137	0.004137125800801989	7.247217368772254E-4	4.141267067869859E-6	1.532268815111848E-4	0.21509327023809263	8.758779848544753E-4
138-139	0.004234445576896931	7.330042710129651E-4	4.141267067869859E-6	1.552975150451197E-4	0.21622383614762108	8.800192519223451E-4
>>END_MODULE
