##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24147199
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.446444947921286	37.0	35.0	39.0	34.0	39.0
2	35.3444951938318	36.0	34.0	38.0	33.0	39.0
3	35.621422509500995	36.0	35.0	38.0	33.0	39.0
4	35.661118790630745	36.0	35.0	38.0	33.0	39.0
5	35.679811641921695	36.0	35.0	38.0	33.0	39.0
6	35.54353335142515	36.0	35.0	38.0	33.0	39.0
7	35.562640909200276	35.0	35.0	38.0	33.0	39.0
8	35.697809215884625	36.0	35.0	38.0	33.0	39.0
9	35.73268166630838	36.0	35.0	38.0	33.0	39.0
10-14	35.58826502403032	36.2	35.0	38.0	33.0	39.0
15-19	35.53341003236028	36.0	35.0	38.0	33.0	39.0
20-24	35.49384711659518	36.0	35.0	38.0	32.8	39.0
25-29	35.47525981791925	36.0	35.0	38.0	33.0	39.0
30-34	35.4433246854014	36.0	35.0	38.0	32.6	39.0
35-39	35.43596340097251	36.0	35.0	38.0	32.6	39.0
40-44	35.3947463637501	36.0	34.8	38.0	32.2	39.0
45-49	35.33854959326753	36.0	34.0	38.0	32.0	39.0
50-54	35.33640049928772	36.0	34.0	38.0	32.0	39.0
55-59	35.35353771673476	36.0	34.2	38.0	32.0	39.0
60-64	35.29208210028832	36.0	34.0	38.0	32.0	39.0
65-69	35.26733199159041	36.0	34.0	38.0	32.0	39.0
70-74	35.22503439839958	36.0	34.0	38.0	32.0	39.0
75-79	35.16075912572717	36.0	34.0	38.0	31.8	39.0
80-84	35.104114485493746	36.0	34.0	38.0	31.8	39.0
85-89	35.04857784954685	36.0	34.0	38.0	31.2	39.0
90-94	34.954150400632386	36.0	34.0	38.0	31.0	39.0
95-99	34.839245893488524	36.0	34.0	38.0	30.6	39.0
100-104	34.76951388026413	36.0	34.0	38.0	30.2	39.0
105-109	34.50430321131656	35.6	34.0	38.0	29.6	39.0
110-114	34.35487419472544	35.4	34.0	38.0	29.0	39.0
115-119	34.251018082884066	35.0	34.0	38.0	28.2	39.0
120-124	34.20193254712483	35.0	34.0	38.0	28.2	39.0
125-129	34.16574179059029	35.0	34.0	38.0	28.4	39.0
130-134	34.09090900356601	35.0	34.0	38.0	28.0	39.0
135-139	33.96499651988622	35.0	33.8	38.0	27.4	39.0
140-144	33.801317378466955	35.0	33.2	38.0	26.8	39.0
145-149	33.76611592093974	35.0	33.0	38.0	26.8	39.0
150	33.51491396579785	35.0	33.0	37.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	18.0
21	312.0
22	2583.0
23	12676.0
24	37821.0
25	80522.0
26	145485.0
27	242537.0
28	366224.0
29	507026.0
30	681928.0
31	909533.0
32	1226518.0
33	1731879.0
34	2598082.0
35	4271709.0
36	9566716.0
37	1765625.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.84616161899358	18.41341101301232	15.746977527290017	31.99344984070409
2	24.245495305687424	25.33138108482065	33.726052450224145	16.69707115926779
3	19.528078598267236	29.009596516763704	30.52763593823035	20.934688946738707
4	23.26849172030263	33.85789382859684	22.69182856363589	20.181785887464628
5	24.97607693546568	34.9010748617262	22.783814387747412	17.339033815060702
6	18.729973608947358	38.619965818809874	23.15872743666874	19.491333135574028
7	20.039156508380124	19.961859758558333	38.785454992109024	21.213528740952523
8	21.38815354940339	22.856733818278467	28.67253050757564	27.082582124742498
9	23.40577472360252	22.782046066709434	29.00824232243251	24.80393688725554
10-14	23.13507770096111	28.694275827585834	25.872874906569958	22.2977715648831
15-19	22.915056938902108	27.821847991562088	27.42034055378431	21.842754515751494
20-24	22.99834278915745	27.907148154119245	27.30519510772243	21.789313949000878
25-29	23.071317712667213	27.865931779499558	27.276361949889093	21.786388557944132
30-34	23.06775042521495	27.631269365858955	27.453487255395544	21.847492953530555
35-39	23.147328376656258	27.61732594838288	27.436136401875316	21.79920927308554
40-44	23.295778529012825	27.402607648199695	27.51210689074124	21.789506932046237
45-49	23.2910177283916	27.243011497938124	27.599110770570118	21.866860003100154
50-54	23.378106090068666	27.24416856795689	27.553588306453264	21.82413703552118
55-59	23.548573894636807	27.130368205438653	27.440038076465928	21.881019823458615
60-64	23.45989630896649	27.109516322034004	27.511430435566712	21.91915693343279
65-69	23.560847977116087	27.032121837668395	27.467415392298005	21.93961479291751
70-74	23.62360951263954	26.992957651113077	27.384619640563695	21.998813195683688
75-79	23.547602353382686	27.047845176577205	27.450119577015954	21.954432893024155
80-84	23.650278444303208	27.08796080241025	27.32381755747323	21.93794319581331
85-89	23.70287584907881	27.002726071872768	27.358896574298324	21.935501504750096
90-94	23.632477621938676	26.94471685929287	27.433571073812747	21.989234444955706
95-99	23.72283612457773	27.086818865300433	27.392642330008066	21.79770268011377
100-104	23.872883144749004	27.07578630548413	27.209716538965868	21.841614010801006
105-109	23.67979656770957	27.220179864339546	27.286679502661986	21.8133440652889
110-114	23.711015923627414	27.232518355441552	27.202982838713506	21.85348288221752
115-119	23.796903317854795	27.24823777697778	27.208223198061194	21.746635707106236
120-124	23.768774324410316	27.15741818413699	27.36992320821975	21.703884283232945
125-129	23.767552501637976	27.246285583681985	27.324493412258704	21.661668502421335
130-134	23.831068140292942	27.18291448364603	27.357368673338623	21.628648702722405
135-139	23.734489018531534	27.193316743582834	27.478145642948416	21.59404859493722
140-144	23.813338547575132	27.338749536447267	27.383896802141706	21.464015113835895
145-149	23.87721775319205	27.631023919471104	27.12190480338054	21.36985352395631
150	23.524007898390202	27.716051041779217	27.18629601719023	21.57364504264035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	1.5
8	3.5
9	4.0
10	4.0
11	5.0
12	8.5
13	14.0
14	24.0
15	43.5
16	70.0
17	119.5
18	193.5
19	333.5
20	540.5
21	837.0
22	1277.5
23	2093.0
24	3456.0
25	5871.5
26	9605.5
27	15782.5
28	28167.0
29	43408.5
30	55853.5
31	72414.5
32	96940.5
33	128478.5
34	173926.0
35	243145.0
36	340825.0
37	459704.5
38	600189.0
39	779287.5
40	988211.0
41	1190899.0
42	1380703.0
43	1560017.0
44	1688853.0
45	1750892.0
46	1757967.5
47	1702835.0
48	1595467.0
49	1452716.0
50	1271472.0
51	1071814.0
52	878394.0
53	700634.5
54	550761.0
55	421873.0
56	315528.5
57	233815.5
58	170285.0
59	123125.0
60	86337.0
61	58400.5
62	39974.5
63	28021.5
64	19470.5
65	13253.5
66	9284.5
67	6981.0
68	6170.0
69	4526.5
70	2228.5
71	1354.5
72	804.5
73	564.0
74	409.0
75	240.0
76	147.0
77	67.5
78	30.5
79	14.5
80	9.5
81	5.5
82	4.0
83	2.0
84	0.5
85	1.5
86	1.0
87	0.5
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.00770524150647866
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.282534135739719E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	8.282534135739719E-7
65-69	8.282534135739719E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	8.282534135739719E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	2.153458875292327E-5
125-129	0.0
130-134	8.282534135739719E-7
135-139	1.6565068271479439E-6
140-144	8.282534135739719E-7
145-149	4.969520481443831E-6
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4147199E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.68281510100168
#Duplication Level	Percentage of total
1	25.194542013370707
2	11.82903078734993
3	7.70559483448724
4	5.471493398473527
5	4.11458651384649
6	3.4070311665224797
7	2.7183404033611627
8	2.2373487517582884
9	1.9799504901169644
>10	22.49923670865066
>50	5.448232287499252
>100	5.914470706399044
>500	0.8501255399853733
>1k	0.5751054939542716
>5k	0.0549109042245884
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.2423801203609579E-5	0.0	0.0	5.466472529588215E-4	0.0
2	1.6565068271479437E-5	4.5553937746568455E-5	0.0	0.0	0.00469619685496442	0.0
3	3.7271403610828735E-5	4.9695204814438316E-5	0.0	0.0	0.006062814987361474	0.0
4	8.282534135739719E-5	4.9695204814438316E-5	0.0	0.0	0.007272064971179473	0.0
5	8.282534135739719E-5	4.9695204814438316E-5	0.0	0.0	0.008713225910798185	0.0
6	9.110787549313691E-5	4.9695204814438316E-5	0.0	4.141267067869859E-6	0.010253777260045772	0.0
7	9.939040962887663E-5	5.797773895017803E-5	0.0	4.141267067869859E-6	0.012005533229754723	0.0
8	1.5736814857905465E-4	6.21190060180479E-5	0.0	4.141267067869859E-6	0.01710757425737039	0.0
9	1.6979194978266422E-4	6.21190060180479E-5	0.0	4.141267067869859E-6	0.022010834465728303	0.0
10-11	1.863570180541437E-4	6.833090661985267E-5	0.0	4.141267067869859E-6	0.027607756907954417	0.0
12-13	2.443347570043217E-4	8.075470782346226E-5	0.0	6.211900601804789E-6	0.03303902866746574	0.0
14-15	2.8781806121695524E-4	9.73197760949417E-5	0.0	8.282534135739719E-6	0.04032144680631489	0.0
16-17	3.1266566362417433E-4	1.0146104316281155E-4	0.0	8.282534135739719E-6	0.0457941312365049	0.0
18-19	3.5200770076893804E-4	1.0974357729855127E-4	0.0	8.282534135739719E-6	0.05281564955007825	0.0
20-21	3.9134973791370175E-4	1.118142108324862E-4	0.0	8.282534135739719E-6	0.057658861385952054	0.0
22-23	4.2862114152453045E-4	1.263086455700307E-4	0.0	8.282534135739719E-6	0.06401777696866623	0.0
24-25	4.803869798729037E-4	1.408030803075752E-4	0.0	8.282534135739719E-6	0.06855246440798371	0.0
26-27	5.093758493479926E-4	1.5736814857905467E-4	0.0	8.282534135739719E-6	0.07200835177612111	0.0
28-29	5.797773895017803E-4	1.6772131624872931E-4	0.0	8.282534135739719E-6	0.0757127151683307	0.0
30-31	7.474987057505096E-4	1.739332168505341E-4	2.0706335339349297E-6	8.282534135739719E-6	0.08007554002433159	2.0706335339349297E-6
32-33	8.55171649515126E-4	1.801451174523389E-4	4.141267067869859E-6	1.6565068271479437E-5	0.08499950656802886	4.141267067869859E-6
34-35	9.297144567367835E-4	1.9256891865594846E-4	4.141267067869859E-6	1.6565068271479437E-5	0.08772031903161936	4.141267067869859E-6
36-37	9.71127127415482E-4	2.1120462046136284E-4	4.141267067869859E-6	1.8635701805414367E-5	0.09012432456451781	4.141267067869859E-6
38-39	0.0010166810651620504	2.2569905519890734E-4	4.141267067869859E-6	2.0706335339349298E-5	0.09323648676602202	4.141267067869859E-6
40-41	0.0010560231023068142	2.4019348993645185E-4	1.2423801203609579E-5	2.0706335339349298E-5	0.09547484161620567	4.141267067869859E-6
42-43	0.001085011971781903	2.526172911400614E-4	1.2423801203609579E-5	2.0706335339349298E-5	0.09793061298745251	4.141267067869859E-6
44-45	0.0011077889406551874	2.6089982527580113E-4	1.2423801203609579E-5	2.4847602407219158E-5	0.09982524267100296	4.141267067869859E-6
46-47	0.001149201611333886	3.0231249595449975E-4	1.2423801203609579E-5	2.4847602407219158E-5	0.10194557140975233	4.141267067869859E-6
48-49	0.0011802611143429099	3.2094819775991407E-4	1.4494434737544507E-5	2.4847602407219158E-5	0.1040659001485017	6.211900601804789E-6
50-51	0.0012092499838179988	3.3130136542958876E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.10596674173265397	8.282534135739719E-6
52-53	0.001238238853293088	3.499370672350031E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.10789864281981525	8.282534135739719E-6
54-55	0.001298287225777201	3.6650213550648255E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.10966282259072781	1.0353167669674649E-5
56-57	0.0013728300329988582	3.727140361082873E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.11148083883352268	1.2423801203609579E-5
58-59	0.0014349490390169063	3.727140361082873E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.11307315602111864	1.2423801203609579E-5
60-61	0.0015157037468403684	3.892791043797668E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.11430518297380993	1.2423801203609579E-5
62-63	0.0015488338833833273	3.9963227204944143E-4	1.8635701805414367E-5	2.8988869475089015E-5	0.11595340726682213	1.6565068271479437E-5
64-65	0.0015674695851887418	4.1826797385485575E-4	2.0706335339349298E-5	2.8988869475089015E-5	0.1174939586160697	1.6565068271479437E-5
66-67	0.0016730718954194232	4.596806445335544E-4	2.0706335339349298E-5	2.8988869475089015E-5	0.11867421973041262	1.6565068271479437E-5
68-69	0.0016979194978266423	4.803869798729037E-4	2.0706335339349298E-5	3.105950300902395E-5	0.1197033245967783	1.6565068271479437E-5
70-71	0.001733120267903536	4.886695140086434E-4	2.0706335339349298E-5	3.3130136542958875E-5	0.12091050394706235	2.4847602407219158E-5
72-73	0.0017579678703107554	5.01093315212253E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.12180708826725617	3.105950300902395E-5
74-75	0.0017745329385822346	5.30082184687342E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.12273059082339116	3.3130136542958875E-5
76-77	0.001789027373319779	5.818480230357152E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.123753483789155	3.3130136542958875E-5
78-79	0.001849075745803892	6.108368925108042E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.12493167426996399	3.52007700768938E-5
80-81	0.001859428913473567	6.232606937144139E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.12624445593047873	3.7271403610828735E-5
82-83	0.0018718527146771764	6.460376625876981E-4	2.6918235941154088E-5	3.3130136542958875E-5	0.12767319306889383	4.348330421263352E-5
84-85	0.0018967003170843955	6.563908302573727E-4	2.8988869475089015E-5	3.3130136542958875E-5	0.12893213825752625	4.9695204814438316E-5
86-87	0.00191533601888981	6.626027308591774E-4	2.8988869475089015E-5	3.3130136542958875E-5	0.1304809721409096	4.9695204814438316E-5
88-89	0.0019277598200934196	6.708852649949173E-4	2.8988869475089015E-5	3.3130136542958875E-5	0.1320111703224875	5.5907105416243096E-5
90-91	0.001942254254830964	6.915916003342665E-4	3.3130136542958875E-5	3.3130136542958875E-5	0.13284149436959541	5.797773895017803E-5
92-93	0.0019505367889667037	7.019447680039412E-4	3.3130136542958875E-5	3.3130136542958875E-5	0.13358899307534594	6.004837248411296E-5
94-95	0.001960889956636378	7.102273021396808E-4	3.3130136542958875E-5	3.3130136542958875E-5	0.13435098621583397	6.21190060180479E-5
96-97	0.0019629605901703134	7.226511033432905E-4	3.3130136542958875E-5	3.52007700768938E-5	0.1350632841515076	6.626027308591775E-5
98-99	0.001975384391373923	7.412868051487048E-4	3.7271403610828735E-5	3.7271403610828735E-5	0.135781793987783	6.833090661985267E-5
100-101	0.0019878081925775327	7.433574386826397E-4	4.348330421263352E-5	4.1412670678698595E-5	0.13644853798571005	7.247217368772254E-5
102-103	0.0019919494596454023	7.454280722165746E-4	4.762457128050339E-5	4.1412670678698595E-5	0.13712977641837465	7.868407428952734E-5
104-105	0.0020064438943829467	7.474987057505096E-4	4.9695204814438316E-5	4.5553937746568455E-5	0.1377840966150981	8.696660842526704E-5
106-107	0.0020416446644598405	7.516399728183795E-4	4.9695204814438316E-5	5.176583834837324E-5	0.13838250970640528	9.110787549313691E-5
108-109	0.00205820973273132	7.619931404880542E-4	4.9695204814438316E-5	5.383647188230817E-5	0.13906374813906985	9.73197760949417E-5
110-111	0.0020789160680706694	7.785582087595335E-4	4.9695204814438316E-5	5.797773895017803E-5	0.1397408453046666	1.1595547790035606E-4
112-113	0.0020892692357403443	7.847701093613383E-4	4.9695204814438316E-5	5.797773895017803E-5	0.14038895360078824	1.263086455700307E-4
114-115	0.0020954811363421487	7.909820099631431E-4	4.9695204814438316E-5	5.797773895017803E-5	0.14098943732562935	1.3044991263790057E-4
116-117	0.0021016930369439535	7.95123277031013E-4	4.9695204814438316E-5	5.797773895017803E-5	0.14167688765889574	1.4287371384151017E-4
118-119	0.0021120462046136284	7.992645440988829E-4	5.383647188230817E-5	6.21190060180479E-5	0.1423312078556192	1.6772131624872931E-4
120-121	0.0021120462046136284	8.034058111667527E-4	5.383647188230817E-5	6.21190060180479E-5	0.1429834574188087	1.7807448391840395E-4
122-123	0.0021120462046136284	8.054764447006876E-4	5.383647188230817E-5	6.21190060180479E-5	0.14357358797598013	1.8221575098627382E-4
124-125	0.002116187471681498	8.116883453024924E-4	6.21190060180479E-5	6.21190060180479E-5	0.14421962563856786	1.9878081925775324E-4
126-127	0.0021327525399529776	8.199708794382323E-4	6.21190060180479E-5	6.21190060180479E-5	0.1450520203192097	2.153458875292327E-4
128-129	0.002147246974690522	8.241121465061021E-4	6.21190060180479E-5	6.21190060180479E-5	0.14582643726090136	2.2569905519890734E-4
130-131	0.0021555295088262617	8.365359477097115E-4	6.418963955198283E-5	6.626027308591775E-5	0.14657186533311795	2.4019348993645185E-4
132-133	0.002180377111233481	8.489597489133212E-4	7.04015401537876E-5	6.833090661985267E-5	0.14721790299570564	2.4226412347038678E-4
134-135	0.0022052247136407	8.55171649515126E-4	7.04015401537876E-5	7.04015401537876E-5	0.14778939785107165	2.5468792467399636E-4
136-137	0.0022114366142425047	8.655248171848006E-4	7.247217368772254E-5	7.04015401537876E-5	0.14847477755080413	2.8367679414908537E-4
138-139	0.0022280016825139844	8.738073513205403E-4	7.454280722165747E-5	7.454280722165747E-5	0.14905662557383986	3.1473629715810935E-4
>>END_MODULE
