##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24057984
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.56315836771693	36.0	35.0	38.0	32.0	39.0
2	35.20857691151511	35.0	34.0	37.0	32.0	39.0
3	35.346216208307396	35.0	34.0	37.0	33.0	39.0
4	35.40643106255287	36.0	34.0	37.0	33.0	39.0
5	35.32013501214399	35.0	34.0	37.0	33.0	39.0
6	35.282227762725256	35.0	34.0	37.0	33.0	39.0
7	35.32514224799551	35.0	34.0	37.0	33.0	39.0
8	35.463189559025395	36.0	34.0	38.0	33.0	39.0
9	35.43477387797747	36.0	34.0	38.0	33.0	39.0
10-14	35.39608159187403	35.4	34.2	37.6	33.0	39.0
15-19	35.36764383915128	35.8	34.0	37.8	33.0	39.0
20-24	35.339406976079125	35.6	34.0	37.6	32.8	39.0
25-29	35.327065185511806	35.8	34.0	37.6	32.6	39.0
30-34	35.311065083425106	35.6	34.0	37.4	32.0	39.0
35-39	35.28449643993445	35.4	34.0	37.0	32.0	39.0
40-44	35.267162161218494	35.6	34.0	37.0	32.0	39.0
45-49	35.2366754920113	35.0	34.0	37.0	32.0	39.0
50-54	35.21695304145185	35.0	34.0	37.0	32.0	39.0
55-59	35.18527766083808	35.0	34.0	37.0	32.0	39.0
60-64	35.14826159997447	35.0	34.0	37.0	32.0	39.0
65-69	35.112282542045094	35.0	34.0	37.0	32.0	39.0
70-74	35.09409307945337	35.0	34.0	37.0	32.0	39.0
75-79	35.04322972365432	35.0	34.0	37.0	32.0	39.0
80-84	34.975440610485066	35.0	34.0	37.0	32.0	39.0
85-89	34.95673308287178	35.0	34.0	37.0	31.4	39.0
90-94	34.90689708663868	35.0	34.0	37.0	31.0	39.0
95-99	34.84639791929365	35.0	34.0	37.0	31.0	39.0
100-104	34.78496959678749	35.0	34.0	37.0	31.0	39.0
105-109	34.729958229251466	35.0	34.0	37.0	31.0	39.0
110-114	34.66580631195033	35.0	34.0	37.0	30.8	39.0
115-119	34.60853576093491	35.0	34.0	37.0	30.0	39.0
120-124	34.5371134006906	35.0	34.0	37.0	30.0	39.0
125-129	34.45812827874522	35.0	34.0	37.0	30.0	39.0
130-134	34.385693132059615	35.0	34.0	37.0	30.0	39.0
135-139	34.29951850495869	35.0	34.0	37.0	29.6	39.0
140-144	34.201498986781274	35.0	34.0	37.0	29.0	39.0
145-149	34.09891409022468	35.0	34.0	37.0	29.0	39.0
150	34.02898243676611	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	26.0
21	296.0
22	2244.0
23	10751.0
24	34679.0
25	82668.0
26	160382.0
27	269517.0
28	392394.0
29	519672.0
30	673085.0
31	882344.0
32	1184114.0
33	1646606.0
34	2454662.0
35	4135869.0
36	1.0057571E7
37	1551096.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.26562746344945	18.15964372408111	16.46658408562347	32.10814472684597
2	24.295726524716287	24.799613300931618	33.54482653243098	17.359833641921117
3	19.684396664325657	28.823013599144463	30.60583131155129	20.886758424978584
4	23.43065819646401	33.71711860810947	22.35841540172277	20.493807793703745
5	25.23201860970562	35.01594314802105	22.366790999611606	17.38524724266173
6	18.6873264193708	38.937747236011134	22.714413643304443	19.660512701313625
7	20.09393638303193	20.23088052598256	38.33297087569765	21.342212215287866
8	21.378146232036734	22.964467845684826	28.380112814107783	27.277273108170657
9	23.381611002052832	22.906633527839805	28.718450752916404	24.993304717190963
10-14	23.021733849533973	28.998598793636233	25.643378311693766	22.336289045136027
15-19	22.911514115230936	28.10177112097173	27.089949016509447	21.896765747287887
20-24	22.988140085235973	28.199340280324108	26.960055635975156	21.85246399846476
25-29	23.105167082994154	28.163000690332158	26.894372363037565	21.83745986363612
30-34	23.0825783241023	27.948779083068644	27.0602840204732	21.908358572355855
35-39	23.180597343484806	27.939454943523113	27.00346629210494	21.876481420887135
40-44	23.348339578245625	27.725158517022873	27.086188102876786	21.84031380185472
45-49	23.337726885178743	27.54013553255335	27.178914076923487	21.943223505344424
50-54	23.421957550557853	27.526673889216983	27.12472749171335	21.92664106851181
55-59	23.60198011603965	27.411583613988604	27.020661415353835	21.96577485461791
60-64	23.519200112528132	27.37878867988274	27.07702025240353	22.024990955185604
65-69	23.59564957728794	27.303012588253445	27.053078927976674	22.04825890648194
70-74	23.663232131171092	27.26250295951648	26.95656710055173	22.1176978087607
75-79	23.590202736854426	27.309676488271005	27.050750387064852	22.04937038780972
80-84	23.685817699453125	27.36598270229923	26.925456643854456	22.022742954393195
85-89	23.719665350507597	27.272599853297763	26.99465636014483	22.01307843604981
90-94	23.656425243278907	27.202193666767755	27.041064621208495	22.100316468744847
95-99	23.73758582597777	27.34094261597314	27.02433753385155	21.89713402419754
100-104	23.885047890962102	27.319464507084216	26.82794036275026	21.967547239203416
105-109	23.661019975738615	27.44661314929796	26.936235388634394	21.956131486329028
110-114	23.696784599214833	27.44679710026241	26.878909944232316	21.97750835629044
115-119	23.781564573324182	27.447388775385335	26.89175867770134	21.879287973589143
120-124	23.750803059807506	27.338937460428937	27.081030563491936	21.82922891627162
125-129	23.7424532329891	27.418789537809985	27.034089805696105	21.80466742350481
130-134	23.78490566790634	27.342144711709842	27.092144545444874	21.780805074938947
135-139	23.703024326560364	27.354984524056547	27.187943096146377	21.754048053236712
140-144	23.731853841119854	27.467236656238526	27.162110507680108	21.638798994961505
145-149	23.80195383997836	27.750523677863075	26.912221658097696	21.53530082406087
150	23.458216615324044	27.75384670635744	27.06060906849053	21.72732760982799
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.5
2	9.0
3	21.0
4	31.0
5	32.0
6	25.0
7	16.5
8	13.0
9	9.0
10	6.5
11	9.0
12	12.5
13	19.5
14	26.0
15	47.5
16	79.5
17	125.0
18	213.0
19	373.0
20	591.0
21	934.5
22	1467.5
23	2395.0
24	3920.0
25	6274.0
26	9986.5
27	16056.5
28	26689.0
29	41235.5
30	54706.5
31	70353.0
32	92317.0
33	120937.5
34	164208.5
35	229002.0
36	322301.5
37	441469.0
38	582529.0
39	761313.5
40	971136.5
41	1171069.5
42	1358158.0
43	1535082.0
44	1665260.0
45	1732426.5
46	1745462.5
47	1702309.0
48	1606773.0
49	1468401.0
50	1286507.0
51	1087459.5
52	895574.0
53	718278.0
54	566975.5
55	437693.0
56	330131.5
57	244202.5
58	176890.5
59	126651.0
60	87832.0
61	59781.5
62	40693.5
63	27751.5
64	18697.5
65	12680.5
66	8996.5
67	6702.0
68	5811.0
69	4437.0
70	2703.0
71	1976.0
72	1157.5
73	883.5
74	768.5
75	468.5
76	302.0
77	109.5
78	28.0
79	4.0
80	1.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.156624262448591E-6
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.156624262448591E-6
10-14	8.313248524897182E-7
15-19	0.0
20-24	0.027000599884013557
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.053300392917378286
85-89	1.6626497049794363E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	8.313248524897182E-7
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.02725415396402292
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4057984E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.549231472347984
#Duplication Level	Percentage of total
1	28.208890160416633
2	10.530751380416296
3	7.078231385290092
4	5.016643332369914
5	3.965424994330376
6	3.1191925723986595
7	2.6379442833795728
8	2.150693650411387
9	1.857028760437682
>10	21.411015292666356
>50	5.618225492923188
>100	6.488999484201978
>500	1.006584654899102
>1k	0.8027341850278699
>5k	0.058964420355085166
>10k+	0.048675950475779425
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.156624262448591E-6	8.313248524897181E-6	4.156624262448591E-6	0.0	0.0010848789324990821	0.0
2	2.0783121312242953E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.004751021531978739	0.0
3	4.1566242624485906E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.006222466520885541	0.0
4	8.728910951142041E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.007511020042244604	0.0
5	8.728910951142041E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.008903489170164881	0.0
6	8.728910951142041E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.010640958111868392	0.0
7	9.975898229876617E-5	3.3252994099588725E-5	4.156624262448591E-6	4.156624262448591E-6	0.012673547376205754	0.0
8	1.6210834623549505E-4	3.3252994099588725E-5	4.156624262448591E-6	4.156624262448591E-6	0.018214327518049724	0.0
9	1.6210834623549505E-4	3.7409618362037316E-5	4.156624262448591E-6	4.156624262448591E-6	0.024183239958925903	0.0
10-11	1.8289146754773801E-4	4.1566242624485906E-5	4.156624262448591E-6	4.156624262448591E-6	0.030335043867349816	0.0
12-13	2.203010859097753E-4	5.1957803280607386E-5	4.156624262448591E-6	4.156624262448591E-6	0.03592362518821195	0.0
14-15	2.4731914361569114E-4	5.8192739674280275E-5	4.156624262448591E-6	4.156624262448591E-6	0.043736000489484075	0.0
16-17	2.701805770591584E-4	6.027105180550457E-5	4.156624262448591E-6	8.313248524897181E-6	0.049559431081174554	0.0
18-19	3.4084318952078444E-4	6.442767606795315E-5	4.156624262448591E-6	8.313248524897181E-6	0.05662361401520593	0.0
20-21	3.990359291950647E-4	6.858430033040175E-5	8.313248524897181E-6	8.313248524897181E-6	0.062081261671800926	0.0
22-23	4.489154203444478E-4	9.1445733773869E-5	1.2469872787345772E-5	8.313248524897181E-6	0.0694696612983033	0.0
24-25	5.174997206748496E-4	9.1445733773869E-5	1.2469872787345772E-5	8.313248524897181E-6	0.07470077293259485	0.0
26-27	5.507527147744383E-4	9.768067016754188E-5	1.2469872787345772E-5	8.313248524897181E-6	0.0786142346756902	0.0
28-29	6.318068878921858E-4	1.1222885508611195E-4	1.2469872787345772E-5	8.313248524897181E-6	0.08259835903124718	0.0
30-31	8.043067947838023E-4	1.1430716721733625E-4	2.0783121312242953E-5	8.313248524897181E-6	0.08782531404127628	0.0
32-33	9.684934531505217E-4	1.2469872787345773E-4	2.0783121312242953E-5	8.313248524897181E-6	0.09398127457396264	0.0
34-35	0.0010952704931552037	1.2677704000468203E-4	2.0783121312242953E-5	8.313248524897181E-6	0.09703223678259991	0.0
36-37	0.0011576198570919325	1.2885535213590633E-4	2.0783121312242953E-5	8.313248524897181E-6	0.09999590988172574	0.0
38-39	0.0012365957180784557	1.3093366426713063E-4	2.0783121312242953E-5	8.313248524897181E-6	0.10334199241299687	0.0
40-41	0.0012843968970966146	1.413252249232521E-4	2.4939745574691544E-5	8.313248524897181E-6	0.10587129827669683	0.0
42-43	0.0013072583305400818	1.6003003410427076E-4	2.4939745574691544E-5	8.313248524897181E-6	0.10857726067155087	0.0
44-45	0.0013405113246396706	1.7873484328528942E-4	2.4939745574691544E-5	8.313248524897181E-6	0.11077611490638617	2.0783121312242953E-6
46-47	0.0013820775672641564	1.9536134033508378E-4	2.4939745574691544E-5	8.313248524897181E-6	0.11298951732614004	4.156624262448591E-6
48-49	0.0014319570584135396	1.9951796459753235E-4	2.4939745574691544E-5	8.313248524897181E-6	0.11515304025474454	4.156624262448591E-6
50-51	0.0014797582374316983	2.0367458885998095E-4	2.4939745574691544E-5	8.313248524897181E-6	0.11712328015514517	4.156624262448591E-6
52-53	0.0015275594164498572	2.1198783738487814E-4	2.4939745574691544E-5	8.313248524897181E-6	0.119299272956537	4.156624262448591E-6
54-55	0.0016003003410427073	2.3900589509079397E-4	2.4939745574691544E-5	8.313248524897181E-6	0.1211718321867701	4.156624262448591E-6
56-57	0.0016564147685857633	2.4731914361569114E-4	2.4939745574691544E-5	8.313248524897181E-6	0.12310466246880869	4.156624262448591E-6
58-59	0.0017083725718663708	2.5563239214058833E-4	2.4939745574691544E-5	8.313248524897181E-6	0.12464053513378345	4.156624262448591E-6
60-61	0.0017977399935090155	2.7018057705915845E-4	2.4939745574691544E-5	1.2469872787345772E-5	0.12601637776465394	4.156624262448591E-6
62-63	0.0018330712997398286	2.868070741089528E-4	2.4939745574691544E-5	1.2469872787345772E-5	0.12785568400078745	4.156624262448591E-6
64-65	0.00185801104531452	3.075901954211957E-4	2.4939745574691544E-5	1.2469872787345772E-5	0.12935206873526894	4.156624262448591E-6
66-67	0.0019349085941698192	3.30451628864663E-4	2.4939745574691544E-5	1.2469872787345772E-5	0.13041408623432454	4.156624262448591E-6
68-69	0.001961926651875735	3.346082531271115E-4	2.9096369837140138E-5	1.2469872787345772E-5	0.13152598322452952	4.156624262448591E-6
70-71	0.001984788085319202	3.4915643804568164E-4	3.3252994099588725E-5	1.2469872787345772E-5	0.13264827177539065	4.156624262448591E-6
72-73	0.0020450591371247067	3.678612472267003E-4	3.3252994099588725E-5	1.2469872787345772E-5	0.13338191595771284	6.234936393672886E-6
74-75	0.0020699988826993984	3.761744957515975E-4	3.7409618362037316E-5	1.2469872787345772E-5	0.1343462527866009	8.313248524897181E-6
76-77	0.0021011735646677627	3.9487930493261616E-4	4.1566242624485906E-5	1.2469872787345772E-5	0.13552049914074266	8.313248524897181E-6
78-79	0.002144818119423473	4.156624262448591E-4	4.1566242624485906E-5	1.2469872787345772E-5	0.13712911273031025	8.313248524897181E-6
80-81	0.002161444616473267	4.36445547557102E-4	4.1566242624485906E-5	1.2469872787345772E-5	0.13843013612445665	1.4548184918570067E-5
82-83	0.0021843060499167344	4.57228668869345E-4	4.1566242624485906E-5	1.2469872787345772E-5	0.1399826352864812	1.6626497049794362E-5
84-85	0.0022071674833602016	4.5930698100056933E-4	4.1566242624485906E-5	1.6626497049794362E-5	0.14143745377833822	1.6626497049794362E-5
86-87	0.0022341855410661178	4.6969854165669075E-4	4.3644554755710205E-5	1.6626497049794362E-5	0.14316660947151683	1.8704809181018658E-5
88-89	0.0022549686623783605	4.759334780503636E-4	4.57228668869345E-5	1.6626497049794362E-5	0.1448749820433832	2.0783121312242953E-5
90-91	0.002279908407953052	4.987949114938309E-4	4.57228668869345E-5	1.6626497049794362E-5	0.1458517887450586	2.0783121312242953E-5
92-93	0.0022902999686091738	5.029515357562795E-4	4.780117901815879E-5	1.6626497049794362E-5	0.14678910751624075	2.0783121312242953E-5
94-95	0.0023110830899214165	5.029515357562795E-4	4.987949114938309E-5	1.6626497049794362E-5	0.14777838409070354	2.286143344346725E-5
96-97	0.002315239714183865	5.091864721499524E-4	5.1957803280607386E-5	2.0783121312242953E-5	0.14861802219171816	2.4939745574691544E-5
98-99	0.0023256312748399866	5.195780328060739E-4	5.611442754305598E-5	2.0783121312242953E-5	0.14945766029273277	2.4939745574691544E-5
100-101	0.0023339445233648837	5.195780328060739E-4	5.8192739674280275E-5	2.0783121312242953E-5	0.15021832253276085	2.4939745574691544E-5
102-103	0.002336022835496108	5.195780328060739E-4	6.027105180550457E-5	2.0783121312242953E-5	0.1511119967491873	2.4939745574691544E-5
104-105	0.002348492708283454	5.195780328060739E-4	6.234936393672887E-5	2.7018057705915842E-5	0.15197033965938292	2.4939745574691544E-5
106-107	0.002371354141726921	5.362045298558683E-4	6.650598819917745E-5	3.3252994099588725E-5	0.15277672476629794	3.3252994099588725E-5
108-109	0.002383824014514267	5.632225875617841E-4	6.650598819917745E-5	3.3252994099588725E-5	0.15355609181550706	4.1566242624485906E-5
110-111	0.00240460713582651	5.736141482179056E-4	6.650598819917745E-5	3.3252994099588725E-5	0.15431883236766636	4.1566242624485906E-5
112-113	0.0024066854479577343	5.777707724803542E-4	6.650598819917745E-5	3.3252994099588725E-5	0.1549963621224455	4.1566242624485906E-5
114-115	0.0024066854479577343	5.819273967428028E-4	6.650598819917745E-5	3.3252994099588725E-5	0.15570298824706175	4.1566242624485906E-5
116-117	0.0024149986964826313	5.819273967428028E-4	6.858430033040175E-5	3.3252994099588725E-5	0.15653223478742026	4.57228668869345E-5
118-119	0.00241915532074508	5.923189573989241E-4	7.481923672407463E-5	3.3252994099588725E-5	0.1572949753395796	4.780117901815879E-5
120-121	0.00241915532074508	5.943972695301485E-4	7.481923672407463E-5	3.3252994099588725E-5	0.15800783640058952	5.403611541183168E-5
122-123	0.0024212336328763044	5.98553893792597E-4	7.481923672407463E-5	3.3252994099588725E-5	0.1587435588950429	5.611442754305598E-5
124-125	0.002431625193532426	6.068671423174942E-4	7.481923672407463E-5	3.3252994099588725E-5	0.1595208476321208	6.650598819917745E-5
126-127	0.002439938442057323	6.151803908423914E-4	7.481923672407463E-5	3.3252994099588725E-5	0.16046855796395906	6.650598819917745E-5
128-129	0.0024440950663197716	6.214153272360644E-4	7.481923672407463E-5	3.3252994099588725E-5	0.16140172011087878	6.650598819917745E-5
130-131	0.0024503300027134446	6.40120136417083E-4	7.481923672407463E-5	3.3252994099588725E-5	0.16220394859353138	6.858430033040175E-5
132-133	0.0024877396210754815	6.421984485483073E-4	7.481923672407463E-5	3.3252994099588725E-5	0.16302280357323373	7.274092459285035E-5
134-135	0.0025106010545189487	6.463550728107558E-4	7.897586098652323E-5	3.5331306230813024E-5	0.16382503205588633	7.897586098652322E-5
136-137	0.0025168359909126217	6.484333849419802E-4	7.897586098652323E-5	3.7409618362037316E-5	0.1647644291391997	8.313248524897181E-5
138-139	0.0025272275515687433	6.54668321335653E-4	7.897586098652323E-5	3.7409618362037316E-5	0.16557289255824592	8.936742164264471E-5
>>END_MODULE
