##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.3.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24221274
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.10518868660666	36.0	34.0	38.0	31.0	39.0
2	34.8693594317128	35.0	34.0	38.0	31.0	39.0
3	35.06603050690067	35.0	34.0	38.0	32.0	39.0
4	35.04724247783168	36.0	34.0	38.0	31.0	39.0
5	34.90324125807751	35.0	34.0	37.0	31.0	39.0
6	34.87428477131302	35.0	34.0	37.0	32.0	39.0
7	34.879917588149986	35.0	34.0	37.0	32.0	39.0
8	35.034956873036485	35.0	34.0	37.0	32.0	39.0
9	35.004008789958775	35.0	34.0	37.0	32.0	39.0
10-14	34.99256045738965	35.2	34.0	37.4	32.0	39.0
15-19	35.00195988864995	35.0	34.0	37.0	32.0	39.0
20-24	34.997209866004575	35.0	34.0	37.0	32.0	39.0
25-29	34.98860416673375	35.0	34.0	37.0	32.0	39.0
30-34	34.9756056432044	35.0	34.0	37.0	32.0	39.0
35-39	34.966146107756344	35.0	34.0	37.0	32.0	39.0
40-44	34.93090242899692	35.0	34.0	37.0	31.6	39.0
45-49	34.859309035519765	35.0	34.0	37.0	31.0	39.0
50-54	34.832872424464554	35.0	34.0	37.0	31.0	39.0
55-59	34.81854459843855	35.0	34.0	37.0	31.0	39.0
60-64	34.796840298326174	35.0	34.0	37.0	31.0	39.0
65-69	34.76573254569516	35.0	34.0	37.0	31.0	39.0
70-74	34.75741154656027	35.0	34.0	37.0	31.0	39.0
75-79	34.73857845792918	35.0	34.0	37.0	31.0	39.0
80-84	34.7247780608072	35.0	34.0	37.0	31.0	39.0
85-89	34.70246314871794	35.0	34.0	37.0	31.0	39.0
90-94	34.680896380595016	35.0	34.0	37.0	31.0	39.0
95-99	34.65964066960309	35.0	34.0	37.0	31.0	39.0
100-104	34.622728201662724	35.0	34.0	37.0	31.0	39.0
105-109	34.592100076981914	35.0	34.0	37.0	30.2	39.0
110-114	34.559889153642374	35.0	34.0	37.0	30.0	39.0
115-119	34.52568609727135	35.0	34.0	37.0	30.0	39.0
120-124	34.484502004312404	35.0	34.0	37.0	30.0	39.0
125-129	34.466780310565	35.0	34.0	37.0	30.0	39.0
130-134	34.4170523069926	35.0	34.0	37.0	30.0	39.0
135-139	34.38216977356352	35.0	34.0	37.0	30.0	39.0
140-144	34.33398564419031	35.0	34.0	37.0	30.0	39.0
145-149	34.2997182476859	35.0	34.0	37.0	30.0	39.0
150	34.28663401437926	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	17.0
22	244.0
23	2657.0
24	17051.0
25	68733.0
26	178141.0
27	335969.0
28	503958.0
29	660348.0
30	826825.0
31	1044382.0
32	1347650.0
33	1799095.0
34	2559259.0
35	4191822.0
36	9376824.0
37	1308291.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.87802941024926	10.727904825257328	14.198969201215991	39.19509656327742
2	22.421145016806328	12.672760603361496	37.054470470001654	27.851623909830526
3	20.868522660498893	17.353927073328645	28.586513622822153	33.19103664335031
4	26.24460772992438	25.19800178735644	22.272392592850096	26.284997889869082
5	25.937773649171696	30.790888166382825	24.424131702533934	18.847206481911545
6	19.194061479563484	36.63440684238783	25.68903952611533	18.482492151933354
7	15.997017165049224	24.708469871480222	40.62338145759842	18.671131505872136
8	18.44638957892854	24.25296433092403	31.712522644904162	25.588123445243273
9	19.670889450957947	22.679424569106192	32.93813033305544	24.711555646880424
10-14	20.331555272343795	29.24845838873724	27.053975703714038	23.36601063520493
15-19	20.500341304165374	27.885200980707385	27.861539900582343	23.75291781454489
20-24	20.67745972922581	28.048626127971545	27.55967431275888	23.714239830043766
25-29	20.675771066770736	28.227707559025323	27.443051038667086	23.65347033553685
30-34	20.653873376663647	27.929731731014517	27.46655561682971	23.94983927549213
35-39	20.723933795649174	27.941798044427767	27.508937607178623	23.825330552744433
40-44	20.795836987050027	28.013440904764085	27.39595524860826	23.794766859577624
45-49	20.87935868699403	27.780164985322344	27.361094666632457	23.979381661051168
50-54	21.004189718779017	27.762856670473713	27.450179086178885	23.78277452456839
55-59	21.003519786998172	27.80867141789084	27.366996230878105	23.820812564232885
60-64	20.94691850630726	27.72182326986858	27.337732342217702	23.99352588160646
65-69	21.04513357306739	27.659138971932475	27.416373092480935	23.8793543625192
70-74	21.17552393249419	27.583369945138152	27.39080094848651	23.850305173881143
75-79	21.191858957454443	27.470516396066863	27.397956945378414	23.93966770110028
80-84	21.219581853771604	27.488356793752818	27.38240272099591	23.90965863147967
85-89	21.210823353737176	27.51298343182397	27.41477051518472	23.861422699254135
90-94	21.212481977387846	27.386134727574134	27.359372265313333	24.042011029724687
95-99	21.28809957329026	27.282309845509822	27.508305684600227	23.92128489659969
100-104	21.34244240270947	27.359624288953977	27.33996304286861	23.957970265467942
105-109	21.317744856114146	27.186022159756206	27.459826460090742	24.03640652403891
110-114	21.403221971510398	27.163787577168268	27.5013976359691	23.931592815352236
115-119	21.441016223402578	27.206256283117398	27.451997453740717	23.900730039739308
120-124	21.391104618042245	27.1300313667588	27.44894311096275	24.029920904236203
125-129	21.416471544595417	27.060857096394987	27.50982818903185	24.012843169977753
130-134	21.50662552660969	27.167830693660537	27.39735880622823	23.928184973501544
135-139	21.46015006387942	27.03601560189788	27.51266218273779	23.991172151484914
140-144	21.541188360676127	26.97182681137357	27.619385952241814	23.867598875708488
145-149	21.544007334383704	27.449335620800536	27.348445733082897	23.65821131173286
150	21.380152011178616	27.020229554674007	27.480094437754953	24.119523996392424
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.5
8	4.0
9	5.5
10	4.5
11	7.5
12	14.0
13	20.0
14	32.5
15	64.5
16	144.0
17	301.0
18	575.5
19	1075.0
20	2022.0
21	3499.5
22	5505.5
23	8840.5
24	14213.5
25	22428.5
26	33290.0
27	46245.5
28	63191.5
29	89180.5
30	121896.0
31	155457.5
32	185547.0
33	218959.0
34	258125.0
35	304586.0
36	376866.0
37	476456.0
38	594207.5
39	741380.0
40	908046.0
41	1069523.5
42	1239748.0
43	1415453.0
44	1558500.0
45	1648696.0
46	1676518.0
47	1641359.5
48	1559531.5
49	1439687.0
50	1281776.0
51	1105642.5
52	925791.5
53	746880.5
54	582249.5
55	443279.0
56	334699.5
57	251798.0
58	189618.5
59	140342.0
60	99252.5
61	70674.0
62	50634.5
63	33981.0
64	22770.0
65	16568.0
66	14329.0
67	10523.5
68	5794.0
69	4133.5
70	4151.0
71	3014.0
72	1321.0
73	422.0
74	182.5
75	102.0
76	64.0
77	43.5
78	16.5
79	2.0
80	3.0
81	2.5
82	0.0
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.3945891533203414E-4
2	1.6514407953933388E-4
3	0.09595283881434147
4	1.1560085567753373E-4
5	9.908644772360033E-5
6	9.082924374663364E-5
7	8.670064175815029E-5
8	1.6927268152781724E-4
9	2.1055870141265074E-4
10-14	2.378074745366408E-4
15-19	2.625790864675409E-4
20-24	3.030393859546777E-4
25-29	3.187280735109144E-4
30-34	3.170766327155211E-4
35-39	1.0734365170056702E-4
40-44	1.9569573425411068E-4
45-49	1.6762124073242392E-4
50-54	1.246837800521971E-4
55-59	1.4697823079000717E-4
60-64	1.246837800521971E-4
65-69	0.03807561897858882
70-74	1.378953064153438E-4
75-79	1.329409840291638E-4
80-84	1.0238932931438702E-4
85-89	8.257203976966694E-5
90-94	1.4367534919922047E-4
95-99	1.3046382283607376E-4
100-104	9.000352334893698E-5
105-109	1.354181452222538E-4
110-114	7.018623380421691E-5
115-119	7.101195420191358E-5
120-124	1.1312369448444373E-4
125-129	1.0899509249596036E-4
130-134	5.94518686341602E-5
135-139	6.440619102034022E-5
140-144	4.706606266871016E-5
145-149	6.605763181573356E-5
150	1.1972945766601707E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4221274E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.55006679408595
#Duplication Level	Percentage of total
1	38.17881971657114
2	10.33590132032645
3	6.376668387285762
4	4.349913978988824
5	3.4371031194110175
6	2.7804947317959368
7	2.273773515994589
8	1.8360206267070749
9	1.5976551874569846
>10	18.37340624655672
>50	4.68159001937225
>100	4.8664404740889
>500	0.5039790273218835
>1k	0.38484050874060327
>5k	0.02339313938176543
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	8.257203976966694E-6	0.0	1.2385805965450042E-5	1.238580596545004E-4	2.8900213919383432E-5
2	8.257203976966694E-6	4.954322386180017E-5	0.0	1.651440795393339E-5	0.00197347175049504	6.19290298272502E-5
3	1.2385805965450042E-5	5.7800427838766865E-5	0.0	2.0643009942416738E-5	0.0030881942873855436	1.0321504971208369E-4
4	6.19290298272502E-5	7.018623380421691E-5	0.0	2.0643009942416738E-5	0.004512561973412299	1.1972945766601707E-4
5	6.605763181573356E-5	7.018623380421691E-5	0.0	2.4771611930900083E-5	0.0058172002017730364	1.4037246760843382E-4
6	8.670064175815029E-5	7.018623380421691E-5	0.0	2.4771611930900083E-5	0.006874122310824773	1.486296715854005E-4
7	1.1147225368905038E-4	1.0734365170056702E-4	0.0	2.4771611930900083E-5	0.008021873663623143	1.486296715854005E-4
8	1.321152636314671E-4	1.0734365170056702E-4	0.0	2.4771611930900083E-5	0.009962316598210317	1.651440795393339E-4
9	1.6927268152781724E-4	1.1560085567753373E-4	0.0	2.4771611930900083E-5	0.013343641626778178	1.651440795393339E-4
10-11	2.126230024068924E-4	1.3005096263722546E-4	0.0	2.8900213919383432E-5	0.018072955204585853	2.2913741036082575E-4
12-13	2.662948282571759E-4	1.321152636314671E-4	0.0	3.302881590786678E-5	0.02192700516083506	2.7661633322838427E-4
14-15	2.745520322341426E-4	1.424367686026755E-4	0.0	3.302881590786678E-5	0.02627442305470802	3.1170945013049274E-4
16-17	3.096451491362511E-4	1.651440795393339E-4	0.0	3.302881590786678E-5	0.030394767839214402	3.302881590786678E-4
18-19	3.3028815907866775E-4	1.8372278848750897E-4	0.0	3.302881590786678E-5	0.034116702531832147	3.344167610671511E-4
20-21	4.087315968598514E-4	2.4565181831475916E-4	0.0	3.302881590786678E-5	0.0376218856200545	3.385453630556345E-4
22-23	5.140109475661767E-4	2.642305272629342E-4	0.0	3.302881590786678E-5	0.04156882912104458	3.468025670326012E-4
24-25	5.821328803761519E-4	2.84873537205351E-4	0.0	3.302881590786678E-5	0.04601739776363539	3.612526739922929E-4
26-27	6.358047062264354E-4	2.890021391938343E-4	0.0	3.302881590786678E-5	0.0502533434038193	3.942814899001597E-4
28-29	6.915408330709607E-4	2.890021391938343E-4	0.0	3.302881590786678E-5	0.05444181012113566	4.1286019884833474E-4
30-31	7.514055619039692E-4	2.890021391938343E-4	0.0	3.302881590786678E-5	0.058663305654359885	4.541462187331682E-4
32-33	7.864986788060776E-4	2.890021391938343E-4	0.0	3.92217188905918E-5	0.062492583998678186	5.346539575085934E-4
34-35	8.463634076390863E-4	2.890021391938343E-4	0.0	4.541462187331682E-5	0.06586358752227484	5.429111614855601E-4
36-37	8.773279225527113E-4	2.890021391938343E-4	0.0	4.954322386180017E-5	0.06904880395638974	5.635541714279769E-4
38-39	8.979709324951281E-4	2.890021391938343E-4	0.0	5.367182585028351E-5	0.07320630615879248	5.75939977393427E-4
40-41	9.309997484029948E-4	2.890021391938343E-4	0.0	5.367182585028351E-5	0.07657318108040065	5.800685793819103E-4
42-43	9.433855543684448E-4	2.993236441650427E-4	0.0	5.367182585028351E-5	0.07986161256422764	5.88325783358877E-4
44-45	9.764143702763117E-4	3.0964514913625103E-4	0.0	6.19290298272502E-5	0.08383539197814285	5.903900843531187E-4
46-47	0.0010135717881726617	3.0964514913625103E-4	0.0	6.19290298272502E-5	0.08771008494433447	5.945186863416021E-4
48-49	0.0010527935070632536	3.2203095510170113E-4	0.0	6.19290298272502E-5	0.0915207845797046	6.234189002609855E-4
50-51	0.0010775651189941535	3.4680256703260116E-4	0.0	6.19290298272502E-5	0.09561016484929735	6.399333082149188E-4
52-53	0.0011291726438501955	3.4680256703260116E-4	0.0	6.19290298272502E-5	0.09959220146718954	6.440619102034021E-4
54-55	0.0011972945766601708	3.529954700153262E-4	0.0	6.19290298272502E-5	0.10255447339392634	6.440619102034021E-4
56-57	0.0012220661885910707	3.6331697498653457E-4	0.0	6.19290298272502E-5	0.10658811753667458	6.605763181573355E-4
58-59	0.0012344519945565208	3.757027809519846E-4	0.0	6.399333082149187E-5	0.11061556877644008	6.667692211400606E-4
60-61	0.0012695451114586292	3.8808858691743466E-4	0.0	6.605763181573356E-5	0.11405469423284671	6.688335221343022E-4
62-63	0.0012943167233895295	3.9634579089440134E-4	0.0	6.812193280997524E-5	0.11727706808485797	6.688335221343022E-4
64-65	0.0013252812383031545	3.9634579089440134E-4	0.0	7.225053479845858E-5	0.12151094942404764	6.729621241227857E-4
66-67	0.0013521171512282963	4.1286019884833474E-4	0.0	7.431483579270025E-5	0.12528655594251564	6.77090726111269E-4
68-69	0.0014264319870209965	4.396961117734765E-4	0.0	7.431483579270025E-5	0.1281352913145692	6.915408330709608E-4
70-71	0.0015358399397158052	4.624034227101349E-4	0.0	7.431483579270025E-5	0.13068470304245763	7.121838430133774E-4
72-73	0.0015936403675545722	4.768535296698266E-4	0.0	7.431483579270025E-5	0.13347770228766662	7.245696489788275E-4
74-75	0.0016266691834624387	4.8511073364679335E-4	0.0	7.84434377811836E-5	0.13639868819451859	7.348911539500358E-4
76-77	0.001651440795393339	4.954322386180016E-4	0.0	8.257203976966694E-5	0.13864258337525928	7.431483579270025E-4
78-79	0.0017092412232321058	4.974965396122434E-4	0.0	1.0734365170056702E-4	0.14089886436196544	7.452126589212443E-4
80-81	0.0017567201460996643	4.99560840606485E-4	0.0	1.0734365170056702E-4	0.14347924060476752	7.493412609097276E-4
82-83	0.0018021347679729812	5.0575374358921E-4	0.0	1.0734365170056702E-4	0.1460038807207251	7.555341638924525E-4
84-85	0.001866128098794473	5.160752485604184E-4	0.0	1.0940795269480871E-4	0.14811772493882858	7.555341638924525E-4
86-87	0.001930121429615965	5.243324525373851E-4	0.0	1.1147225368905038E-4	0.1501489971171624	7.555341638924525E-4
88-89	0.0019858575564604903	5.243324525373851E-4	0.0	1.2385805965450043E-4	0.1521410475766056	7.617270668751776E-4
90-91	0.00201062916839139	5.346539575085934E-4	4.128601988483347E-6	1.2798666164298377E-4	0.15472555242139616	7.720485718463859E-4
92-93	0.002076686800207124	5.387825594970768E-4	4.128601988483347E-6	1.2798666164298377E-4	0.1573224430721522	7.720485718463859E-4
94-95	0.0021406801310286157	5.491040644682852E-4	4.128601988483347E-6	1.2798666164298377E-4	0.16016704984221722	7.761771738348693E-4
96-97	0.002252152384717666	5.635541714279769E-4	4.128601988483347E-6	1.2798666164298377E-4	0.16242333082892335	7.803057758233526E-4
98-99	0.0023182100165334	5.69747074410702E-4	4.128601988483347E-6	1.321152636314671E-4	0.16440299548240112	7.803057758233526E-4
100-101	0.0023801390463606496	5.841971813703936E-4	4.128601988483347E-6	1.3624386561995045E-4	0.16645078206868885	7.803057758233526E-4
102-103	0.0024007820563030666	5.88325783358877E-4	6.192902982725021E-6	1.3624386561995045E-4	0.16823433812771368	7.803057758233526E-4
104-105	0.0024420680761879	5.924543853473603E-4	8.257203976966694E-6	1.3830816661419213E-4	0.1699208720400091	7.90627280794561E-4
106-107	0.0025081257080036335	5.965829873358437E-4	8.257203976966694E-6	1.424367686026755E-4	0.17145877628071915	7.926915817888027E-4
108-109	0.002574183339819367	5.986472883300854E-4	8.257203976966694E-6	1.5275827357388386E-4	0.17302351643435437	7.96820183777286E-4
110-111	0.0026484981756120674	6.110330942955353E-4	8.257203976966694E-6	1.5275827357388386E-4	0.1745903208889838	7.96820183777286E-4
112-113	0.002726941613393251	6.172259972782604E-4	8.257203976966694E-6	1.5482257456812554E-4	0.17613441803267657	8.009487857657694E-4
114-115	0.0027785491382492926	6.296118032437105E-4	8.257203976966694E-6	1.5895117655660885E-4	0.17800673903445374	8.009487857657694E-4
116-117	0.002830156663105335	6.358047062264355E-4	8.257203976966694E-6	1.6927268152781724E-4	0.17952812886720987	8.071416887484944E-4
118-119	0.002920985906851968	6.523191141803689E-4	8.257203976966694E-6	1.6927268152781724E-4	0.181049518699966	8.09205989742736E-4
120-121	0.0030076865486101186	6.564477161688522E-4	8.257203976966694E-6	1.713369825220589E-4	0.1825110438038891	8.09205989742736E-4
122-123	0.003168702026160969	6.585120171630938E-4	8.257203976966694E-6	1.8165848749326729E-4	0.1839085755769907	8.09205989742736E-4
124-125	0.0032843028818385026	6.605763181573356E-4	8.257203976966694E-6	1.8372278848750894E-4	0.1852813357381614	8.09205989742736E-4
126-127	0.0033359104066945445	6.750264251170273E-4	8.257203976966694E-6	1.8578708948175062E-4	0.18657358816055672	8.133345917312194E-4
128-129	0.00341641814546997	6.77090726111269E-4	8.257203976966694E-6	1.9404429345871733E-4	0.18781216875710172	8.133345917312194E-4
130-131	0.0035010544862338787	6.874122310824774E-4	1.0321504971208367E-5	2.02301497435684E-4	0.18902391344072156	8.174631937197028E-4
132-133	0.003587755127992029	6.936051340652023E-4	1.2385805965450042E-5	2.105587014126507E-4	0.1901840505994854	8.174631937197028E-4
134-135	0.0037528992075313624	6.956694350594441E-4	1.2385805965450042E-5	2.105587014126507E-4	0.19120587959163504	8.174631937197028E-4
136-137	0.003953136403972805	7.08055241024894E-4	1.2385805965450042E-5	2.105587014126507E-4	0.19231028062355432	8.174631937197028E-4
138-139	0.004017129734794297	7.348911539500358E-4	1.2385805965450042E-5	2.126230024068924E-4	0.19343738896641027	8.277846986909111E-4
>>END_MODULE
