##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24221274
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.85799892276517	37.0	35.0	38.0	33.0	39.0
2	35.364516292578166	35.0	34.0	37.0	33.0	39.0
3	35.49259807721097	35.0	34.0	37.0	33.0	39.0
4	35.56601576779157	36.0	35.0	38.0	33.0	39.0
5	35.488540115602504	36.0	35.0	38.0	33.0	39.0
6	35.41326620556788	35.0	35.0	37.0	33.0	39.0
7	35.45107619029453	35.0	34.0	38.0	33.0	39.0
8	35.618532121803334	36.0	35.0	38.0	33.0	39.0
9	35.59282339979309	36.0	35.0	38.0	33.0	39.0
10-14	35.552586928334165	35.8	35.0	37.8	33.0	39.0
15-19	35.521900160990704	36.0	35.0	38.0	33.0	39.0
20-24	35.497039709802216	36.0	35.0	38.0	33.0	39.0
25-29	35.483570005442324	36.0	34.8	38.0	33.0	39.0
30-34	35.466828359234945	36.0	34.2	38.0	33.0	39.0
35-39	35.44122794696926	36.0	34.0	38.0	33.0	39.0
40-44	35.424726056936564	36.0	34.0	38.0	33.0	39.0
45-49	35.393513561673096	36.0	34.0	38.0	32.8	39.0
50-54	35.37374541900645	36.0	34.0	38.0	32.0	39.0
55-59	35.34216332303577	36.0	34.0	38.0	32.0	39.0
60-64	35.310137963841214	36.0	34.0	38.0	32.0	39.0
65-69	35.27469111657793	36.0	34.0	38.0	32.0	39.0
70-74	35.253183007632046	36.0	34.0	38.0	32.0	39.0
75-79	35.205788836706105	36.0	34.0	38.0	32.0	39.0
80-84	35.14514152310898	36.0	34.0	38.0	32.0	39.0
85-89	35.11962830691729	36.0	34.0	38.0	32.0	39.0
90-94	35.0740336945117	36.0	34.0	38.0	32.0	39.0
95-99	35.01351314551002	35.2	34.0	38.0	31.4	39.0
100-104	34.95640596774554	35.2	34.0	38.0	31.0	39.0
105-109	34.89784854421778	35.0	34.0	37.6	31.0	39.0
110-114	34.837053955130514	35.0	34.0	37.6	31.0	39.0
115-119	34.78071839656329	35.0	34.0	37.0	31.0	39.0
120-124	34.70909667261928	35.0	34.0	37.0	30.2	39.0
125-129	34.63712707267174	35.0	34.0	37.0	30.0	39.0
130-134	34.564557644655686	35.0	34.0	37.0	30.0	39.0
135-139	34.48079929239064	35.0	34.0	37.0	30.0	39.0
140-144	34.380241320089105	35.0	34.0	37.0	29.8	39.0
145-149	34.291188704607364	35.0	34.0	37.0	29.0	39.0
150	34.222518931085126	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	31.0
21	315.0
22	2410.0
23	10686.0
24	33969.0
25	79890.0
26	155685.0
27	264587.0
28	378638.0
29	489683.0
30	620135.0
31	797589.0
32	1052220.0
33	1459411.0
34	2189949.0
35	3779700.0
36	1.0842448E7
37	2063911.0
38	16.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.8019626878421	18.815376928562884	15.840834796716308	31.54182558687871
2	24.254043129772743	25.811711052511566	33.16947461844801	16.764771199267685
3	19.496877001597852	29.732114834256862	30.134963173283126	20.636044990862164
4	22.99975632991064	34.439055517888946	22.450978424999445	20.110209727200974
5	24.96375293884211	35.36415136544841	22.612732922306233	17.05936277340325
6	18.702405992352013	39.07777105366134	23.008921826325075	19.210901127661575
7	20.193859332089634	20.464431392006876	38.48289317894674	20.85881609695675
8	21.41993852181351	22.984307101269735	28.7731768361978	26.822577540718957
9	23.47847598767926	22.84058220884665	29.080398495966808	24.600543307507277
10-14	23.13628754622899	28.91559296179053	25.854046323079455	22.094073168901023
15-19	22.950400544579118	28.0954156251236	27.275149936374117	21.67903389392317
20-24	23.028485119694363	28.24037545635741	27.08757519899948	21.643564224948747
25-29	23.145726356095057	28.214960121420535	27.00393629170786	21.635377230776548
30-34	23.16065703232621	27.988581442908412	27.13878221269451	21.711979312070866
35-39	23.25202216861095	27.988397307259728	27.083993187146145	21.67558733698318
40-44	23.399872360140925	27.74505585461772	27.192624962667118	21.66244682257424
45-49	23.409188137667737	27.54667818051189	27.2783818060107	21.76575187580967
50-54	23.487167082741678	27.51340623145432	27.24807501451947	21.751351671284535
55-59	23.661662057908266	27.401422402471482	27.128553188407846	21.80836235121241
60-64	23.597396239355536	27.354330742470438	27.181792336769732	21.86648068140429
65-69	23.681495036140543	27.26993881494425	27.13675341767737	21.911812731237838
70-74	23.72469755306843	27.23312902533533	27.050226177202735	21.991947244393504
75-79	23.66516971815768	27.283443472048578	27.12999076762023	21.921396042173505
80-84	23.763040183360413	27.329068861634525	27.007635515416396	21.900255439588665
85-89	23.800637603130518	27.24619792704709	27.057083182801062	21.896081287021328
90-94	23.710144231059026	27.171195866906096	27.142023990975865	21.976635911059013
95-99	23.808945805245422	27.302781843762634	27.11629454338364	21.7719778076083
100-104	23.970989304691404	27.2808564900426	26.903040690592906	21.84511351467309
105-109	23.740505970082335	27.43620257134286	26.99964915140302	21.823642307171788
110-114	23.76033647115342	27.42619814300437	26.93909246887674	21.874372916965473
115-119	23.850808177967846	27.4278718782505	26.952266012101596	21.76905393168006
120-124	23.8362342129485	27.31840034508507	27.146032037786288	21.699333404180145
125-129	23.796693765984397	27.41486430482559	27.08829271325695	21.700149215933067
130-134	23.842452713263555	27.31937221799316	27.16867246537073	21.669502603372557
135-139	23.765523646691747	27.330521920523253	27.261793908941375	21.642160523843625
140-144	23.807215921012247	27.460264063731742	27.221893447883872	21.51062656737214
145-149	23.892313562013626	27.885792395845073	26.837398840571268	21.384495201570033
150	23.508321651454008	27.84073620570082	27.004582830779256	21.646359312065915
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	3.5
3	10.5
4	18.0
5	17.5
6	13.5
7	13.0
8	10.0
9	10.0
10	8.5
11	6.5
12	10.5
13	18.5
14	27.5
15	39.0
16	74.5
17	136.0
18	210.5
19	370.5
20	607.0
21	940.5
22	1480.0
23	2461.5
24	4008.0
25	6559.0
26	10412.0
27	16498.0
28	27941.5
29	43512.0
30	58118.0
31	75408.0
32	99414.0
33	130773.0
34	176664.5
35	246111.5
36	343504.5
37	465517.5
38	611819.0
39	795084.5
40	1007260.5
41	1207408.0
42	1392199.5
43	1560870.0
44	1679239.0
45	1737356.5
46	1742147.0
47	1693603.0
48	1593627.0
49	1450620.5
50	1267945.5
51	1069612.0
52	879381.5
53	704870.5
54	554374.5
55	425093.5
56	319418.5
57	236518.5
58	171541.0
59	123030.0
60	85986.5
61	58822.5
62	40473.5
63	28261.0
64	19599.0
65	13565.0
66	9792.5
67	7698.5
68	7404.0
69	5782.0
70	3250.0
71	2340.5
72	1400.0
73	1018.5
74	858.5
75	567.0
76	365.5
77	104.5
78	23.5
79	4.0
80	2.5
81	2.5
82	1.5
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.128601988483347E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.01952168164234466
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	1.6514407953933389E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.038342326667044846
85-89	8.257203976966694E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.019089829874349304
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4221274E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.727972368669775
#Duplication Level	Percentage of total
1	27.369389964649383
2	10.625842306471974
3	6.991045830499389
4	5.076956713679868
5	3.79042586766069
6	3.164156805727842
7	2.5883188537120345
8	2.1976358345949323
9	1.8539072603996245
>10	21.80232549550518
>50	5.453159421340495
>100	6.760051980216073
>500	1.1232816759030395
>1k	1.0076703652738612
>5k	0.09498816649185364
>10k+	0.10084345787380306
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.257203976966694E-6	4.128601988483347E-6	0.0	0.0	9.7435006928207E-4	0.0
2	1.651440795393339E-5	1.2385805965450042E-5	0.0	0.0	0.0052515817293508175	0.0
3	4.954322386180017E-5	1.651440795393339E-5	0.0	0.0	0.006836964892928423	0.0
4	9.4957845735117E-5	1.651440795393339E-5	0.0	0.0	0.008306747200828494	0.0
5	9.908644772360033E-5	1.651440795393339E-5	0.0	0.0	0.00983020133457885	0.0
6	9.908644772360033E-5	1.651440795393339E-5	0.0	0.0	0.011448613314064321	0.0
7	1.1972945766601707E-4	4.1286019884833476E-5	0.0	0.0	0.013484014094386612	0.0
8	1.651440795393339E-4	4.541462187331682E-5	0.0	0.0	0.019036983768896714	0.0
9	1.651440795393339E-4	4.954322386180017E-5	0.0	0.0	0.024978042030324252	0.0
10-11	2.2294450737810075E-4	5.573612684452519E-5	0.0	0.0	0.03160031961985154	0.0
12-13	2.5390902229172584E-4	5.7800427838766865E-5	4.128601988483347E-6	0.0	0.03746706304548637	0.0
14-15	2.8487353720535094E-4	5.9864728833008534E-5	4.128601988483347E-6	0.0	0.04539810746536289	0.0
16-17	3.075808481420094E-4	6.19290298272502E-5	4.128601988483347E-6	0.0	0.05125246508503228	0.0
18-19	3.674455769750179E-4	7.225053479845858E-5	4.128601988483347E-6	2.0643009942416736E-6	0.05858899081856718	0.0
20-21	4.04602994871368E-4	8.67006417581503E-5	4.128601988483347E-6	6.192902982725021E-6	0.06454243488596018	0.0
22-23	4.8098213165830993E-4	9.908644772360033E-5	1.2385805965450042E-5	8.257203976966694E-6	0.07257463005455453	0.0
24-25	5.532326664567685E-4	1.0734365170056702E-4	1.2385805965450042E-5	8.257203976966694E-6	0.07811727822409342	0.0
26-27	6.13097395289777E-4	1.1972945766601707E-4	1.2385805965450042E-5	8.257203976966694E-6	0.08198164968531382	0.0
28-29	7.658556688636609E-4	1.238580596545004E-4	1.2385805965450042E-5	8.257203976966694E-6	0.08597813641016572	0.0
30-31	9.929287782302451E-4	1.2798666164298375E-4	1.2385805965450042E-5	8.257203976966694E-6	0.0915207845797046	0.0
32-33	0.0011374298478271623	1.3624386561995045E-4	1.2385805965450042E-5	1.2385805965450042E-5	0.09782515981611867	0.0
34-35	0.0012427091985334877	1.3624386561995045E-4	1.2385805965450042E-5	1.2385805965450042E-5	0.10130350699141588	0.0
36-37	0.001314959733331946	1.3624386561995045E-4	1.651440795393339E-5	1.2385805965450042E-5	0.1045733597662947	0.0
38-39	0.0013665672581879879	1.3830816661419213E-4	1.651440795393339E-5	1.2385805965450042E-5	0.1080104209217071	0.0
40-41	0.0014016603750900965	1.424367686026755E-4	1.651440795393339E-5	1.2385805965450042E-5	0.11069401221422126	0.0
42-43	0.0014243676860267549	1.5482257456812552E-4	1.651440795393339E-5	1.2385805965450042E-5	0.11339205361369514	2.0643009942416736E-6
44-45	0.0014594608029288633	1.713369825220589E-4	1.651440795393339E-5	1.2385805965450042E-5	0.11556369825963737	4.128601988483347E-6
46-47	0.0015151969297733884	2.3326601234930912E-4	1.8578708948175065E-5	1.2385805965450042E-5	0.11805118095769859	4.128601988483347E-6
48-49	0.001575061658606397	2.4358751732051748E-4	2.0643009942416738E-5	1.2385805965450042E-5	0.12024346861358326	6.192902982725021E-6
50-51	0.0016535050963875806	2.5390902229172584E-4	2.0643009942416738E-5	1.2385805965450042E-5	0.12222519756805525	8.257203976966694E-6
52-53	0.0017360771361572477	2.642305272629342E-4	2.0643009942416738E-5	1.651440795393339E-5	0.12422137662948696	8.257203976966694E-6
54-55	0.0017814917580305644	2.931307411823176E-4	2.0643009942416738E-5	1.651440795393339E-5	0.12592029634774787	8.257203976966694E-6
56-57	0.0018454850888520564	3.240952560959428E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.1276914666008072	8.257203976966694E-6
58-59	0.0018826425067484064	3.3235246007290946E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.12943580094094143	8.257203976966694E-6
60-61	0.00196108594452959	3.447382660383595E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.1307837894901812	8.257203976966694E-6
62-63	0.001983793255466248	3.5918837299805126E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.1326746892009066	8.257203976966694E-6
64-65	0.002006500566402907	3.674455769750179E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.13433645150127116	8.257203976966694E-6
66-67	0.0021097156161149905	4.0253869387712636E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.1354924600580465	1.8578708948175065E-5
68-69	0.002157194538982549	4.2318170381954313E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.13663608280885636	2.0643009942416738E-5
70-71	0.002184030451907691	4.355675097849932E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.1379345281342344	2.0643009942416738E-5
72-73	0.002214994966821316	4.541462187331682E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.13884282057170072	2.4771611930900087E-5
74-75	0.0022377022777579744	4.644677237043766E-4	2.4771611930900083E-5	2.0643009942416738E-5	0.1399121284867179	2.8900213919383432E-5
76-77	0.0022666024916773576	4.9336793762376E-4	2.4771611930900083E-5	2.0643009942416738E-5	0.14112180886934353	2.8900213919383432E-5
78-79	0.0023285315215046077	5.222681515431435E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.14252553354542785	2.8900213919383432E-5
80-81	0.0023450459294585414	5.284610545258684E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.14390242230858707	2.8900213919383432E-5
82-83	0.002353303133435508	5.449754624798019E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.14554767020099768	2.8900213919383432E-5
84-85	0.0023698175413894413	5.491040644682852E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.14708557444170772	3.302881590786678E-5
86-87	0.002386331949343375	5.552969674510102E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.14876178684903196	3.302881590786678E-5
88-89	0.002398717755308825	5.718113754049437E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15040703474144257	3.7157417896350124E-5
90-91	0.002409039260280033	6.027758903185687E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15123894804212198	3.7157417896350124E-5
92-93	0.002411103561274275	6.06904492307052E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15215962628555377	3.7157417896350124E-5
94-95	0.002421425066245483	6.13097395289777E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.1531628765687552	3.7157417896350124E-5
96-97	0.0024234893672397248	6.213545992667438E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15405671889926187	4.1286019884833476E-5
98-99	0.002427617969228208	6.234189002609855E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15493404682181455	4.1286019884833476E-5
100-101	0.0024317465712166914	6.254832012552271E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15566274507278188	4.954322386180017E-5
102-103	0.002437939474199417	6.337404052321938E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15636460741082406	6.19290298272502E-5
104-105	0.002448260979170625	6.378690072206772E-4	2.8900213919383432E-5	2.4771611930900083E-5	0.15717794200255528	6.19290298272502E-5
106-107	0.0024812897950784916	6.564477161688522E-4	2.8900213919383432E-5	2.4771611930900083E-5	0.15791283315650528	6.399333082149187E-5
108-109	0.002485418397066975	6.688335221343023E-4	2.8900213919383432E-5	2.4771611930900083E-5	0.1587199748452538	6.812193280997524E-5
110-111	0.0024957399020381836	6.812193280997523E-4	3.302881590786678E-5	2.4771611930900083E-5	0.15957459545686986	7.431483579270025E-5
112-113	0.002506061407009392	6.89476532076719E-4	3.302881590786678E-5	2.4771611930900083E-5	0.16029710080485443	7.637913678694192E-5
114-115	0.0025081257080036335	6.977337360536857E-4	3.302881590786678E-5	2.4771611930900083E-5	0.1609638700259945	8.050773877542528E-5
116-117	0.002510190008997875	7.121838430133774E-4	3.509311690210845E-5	2.4771611930900083E-5	0.1618061048316451	8.463634076390862E-5
118-119	0.002512254309992117	7.266339499730691E-4	3.7157417896350124E-5	2.4771611930900083E-5	0.16258021770448572	8.670064175815029E-5
120-121	0.0025163829119806	7.348911539500359E-4	3.7157417896350124E-5	2.4771611930900083E-5	0.16328827294551063	9.082924374663364E-5
122-123	0.0025205115139690835	7.410840569327608E-4	3.7157417896350124E-5	2.4771611930900083E-5	0.16395710646764494	9.702214672935866E-5
124-125	0.0025267044169518084	7.472769599154859E-4	3.7157417896350124E-5	2.6835912925141756E-5	0.16484888449715734	1.01150748717842E-4
126-127	0.0025432188249057417	7.823700768175943E-4	3.7157417896350124E-5	2.8900213919383432E-5	0.1658830992952724	1.0940795269480871E-4
128-129	0.0025576689318654337	7.885629798003193E-4	3.7157417896350124E-5	2.8900213919383432E-5	0.166793456033733	1.2385805965450043E-4
130-131	0.002567990436836642	8.030130867600111E-4	3.7157417896350124E-5	2.8900213919383432E-5	0.16769142696622813	1.321152636314671E-4
132-133	0.002588633446779059	8.09205989742736E-4	3.7157417896350124E-5	2.8900213919383432E-5	0.1685130187619363	1.3830816661419213E-4
134-135	0.0026030835537387506	8.09205989742736E-4	4.1286019884833476E-5	3.302881590786678E-5	0.1692602957218518	1.4450106959691716E-4
136-137	0.002613405058709959	8.15398892725461E-4	4.335032087907515E-5	3.7157417896350124E-5	0.17010459482849666	1.486296715854005E-4
138-139	0.0026361123696466175	8.277846986909111E-4	4.541462187331682E-5	3.7157417896350124E-5	0.17087664340034303	1.5069397257964218E-4
>>END_MODULE
