##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.S.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24154014
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.58475878998828	37.0	35.0	39.0	34.0	39.0
2	35.470902020674494	36.0	35.0	38.0	33.0	39.0
3	35.76026589203765	36.0	35.0	38.0	34.0	39.0
4	35.76378274021039	37.0	35.0	38.0	33.0	39.0
5	35.812766979434556	36.0	35.0	38.0	33.0	39.0
6	35.684593003879186	36.0	35.0	38.0	33.0	39.0
7	35.683135523561425	36.0	35.0	38.0	33.0	39.0
8	35.756864925225265	36.0	35.0	38.0	33.0	39.0
9	35.85039501094932	37.0	35.0	39.0	33.0	39.0
10-14	35.70039975964243	36.4	35.0	38.4	33.0	39.0
15-19	35.65006807564159	36.0	35.0	38.0	33.0	39.0
20-24	35.62737584734363	36.0	35.0	38.0	33.0	39.0
25-29	35.59532403185657	36.0	35.0	38.0	33.0	39.0
30-34	35.56682717829012	36.0	35.0	38.0	33.0	39.0
35-39	35.56299993036354	36.0	35.0	38.0	33.0	39.0
40-44	35.56473300876617	36.0	35.0	38.0	33.0	39.0
45-49	35.46416452354461	36.0	35.0	38.0	32.6	39.0
50-54	35.476055391869856	36.0	34.8	38.0	32.8	39.0
55-59	35.50486540249583	36.0	35.0	38.0	32.6	39.0
60-64	35.43212223028438	36.0	34.8	38.0	32.0	39.0
65-69	35.4019513692424	36.0	34.4	38.0	32.0	39.0
70-74	35.385223482937455	36.0	34.0	38.0	32.0	39.0
75-79	35.29613561538881	36.0	34.0	38.0	32.0	39.0
80-84	35.25383964752194	36.0	34.0	38.0	32.0	39.0
85-89	35.184032558729164	36.0	34.0	38.0	31.8	39.0
90-94	35.10098040019352	36.0	34.0	38.0	31.2	39.0
95-99	34.994040361159016	36.0	34.0	38.0	31.0	39.0
100-104	34.930437508233624	36.0	34.0	38.0	30.8	39.0
105-109	34.663037795705506	36.0	34.0	38.0	29.8	39.0
110-114	34.5016468153078	36.0	34.0	38.0	29.4	39.0
115-119	34.39806008227038	36.0	34.0	38.0	29.0	39.0
120-124	34.36313228931638	36.0	34.0	38.0	28.8	39.0
125-129	34.32089278411448	35.8	34.0	38.0	28.6	39.0
130-134	34.24924543804603	35.4	34.0	38.0	28.4	39.0
135-139	34.149073441789014	35.0	34.0	38.0	28.0	39.0
140-144	33.97531884348498	35.0	33.8	38.0	27.4	39.0
145-149	33.95874212874101	35.0	34.0	38.0	27.2	39.0
150	33.727215857372606	35.0	33.0	38.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	15.0
21	220.0
22	1959.0
23	9761.0
24	30007.0
25	66050.0
26	123450.0
27	211991.0
28	326298.0
29	462873.0
30	630896.0
31	846996.0
32	1152291.0
33	1644543.0
34	2496248.0
35	4138291.0
36	9567675.0
37	2444447.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.54745451418551	18.259644132027084	14.661799069918565	32.53110228386884
2	24.213180467644012	25.577752832303567	33.01395370558285	17.195112994469575
3	19.72229957306475	28.982400192365542	29.737049088404106	21.558251146165603
4	23.600909563106157	33.912636632569644	21.73967440774026	20.74677939658394
5	25.481599041881818	34.776430948495765	21.81972735463348	17.922242654988942
6	19.10242330736415	38.735743880913546	22.13107519106348	20.030757620658825
7	20.4835726268934	20.160065320819967	37.256109895440154	22.10025215684648
8	21.648716441085114	23.12248804691427	28.254587415574072	26.974208096426537
9	23.61101140373604	23.073552081239995	27.917811093427368	25.397625421596594
10-14	23.308614460519895	28.409403919365122	25.291736603282583	22.9902450168324
15-19	23.247288835718983	27.392462387411054	26.8630944736556	22.497154303214366
20-24	23.1972027506484	27.321668357068933	26.768240674200154	22.71288821808251
25-29	23.253332551682714	26.99390585763509	26.761305180993933	22.99145640968826
30-34	23.100715268277977	26.735586060354194	26.979178698828278	23.184519972539555
35-39	23.376924431690732	26.52149493661799	27.037344600363316	23.06423603132796
40-44	23.571239960364352	26.158310581421375	27.06444982602064	23.205999632193638
45-49	23.458240108662682	26.00320841082563	27.134933348966346	23.40361813154534
50-54	23.6954147662579	26.068767700474133	26.829322861202282	23.406494672065687
55-59	23.736646836422302	26.075394342323392	26.86208097751372	23.32587784374059
60-64	23.441289716897572	26.076920382674285	26.941544374363612	23.54024552606453
65-69	23.652846272259342	25.998276725350912	26.945901414150047	23.402975588239702
70-74	23.790403532928316	26.002370454865183	26.6507703440099	23.556455668196598
75-79	23.636556640233795	26.09899042039141	26.88967804688695	23.37477489248785
80-84	23.942105854538298	26.10455802501398	26.596257665496093	23.35707845495163
85-89	23.711334469801454	26.15895499736777	26.80567318380848	23.324037349022298
90-94	23.727925304671928	25.96665713615965	26.81077025127169	23.49464730789673
95-99	23.753641113232774	26.141295604117808	26.818566056970905	23.286497225678517
100-104	23.991413601068544	25.964987020376824	26.7479434267116	23.295655951843035
105-109	23.784568674875892	26.20862458892857	26.60482209379213	23.40198464240341
110-114	23.89270122970037	26.311774100983794	26.473636224604324	23.321888444711508
115-119	24.01937665515968	26.42501159434618	26.64787972715425	22.90773202333989
120-124	23.989652403116104	26.57245292645769	26.745164592518663	22.692730077907548
125-129	23.96497824336775	26.623561615887116	26.595533148237806	22.815926992507332
130-134	24.09771808528388	26.63880628702128	26.628745019357858	22.634730608336984
135-139	23.87497581147382	26.60772077055184	26.93065425895671	22.58664915901763
140-144	23.939890073757514	26.78643143951146	26.634294407546506	22.63938407918452
145-149	24.124490646767786	27.220651832201902	26.227411528596544	22.42744599243377
150	23.911922051548036	27.300584490842805	26.133999922331753	22.65349353527741
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.5
9	2.0
10	2.0
11	4.0
12	7.5
13	13.0
14	18.0
15	24.5
16	35.0
17	75.0
18	134.5
19	216.0
20	353.5
21	516.0
22	784.0
23	1314.0
24	2237.5
25	3713.0
26	5734.0
27	8968.0
28	13732.0
29	19842.0
30	28762.0
31	42143.5
32	61633.0
33	86206.5
34	119431.0
35	171688.5
36	246716.0
37	339721.0
38	457245.0
39	599547.0
40	769928.0
41	959796.5
42	1153423.5
43	1351595.0
44	1507695.5
45	1603520.5
46	1638392.5
47	1618238.0
48	1574646.0
49	1518622.5
50	1407554.5
51	1248132.5
52	1070612.0
53	899562.0
54	762921.0
55	658680.5
56	544958.0
57	426607.0
58	350668.0
59	287927.5
60	215370.0
61	143318.5
62	90354.0
63	57339.5
64	36240.5
65	18754.0
66	10616.0
67	6432.5
68	4260.5
69	2826.5
70	1424.0
71	947.5
72	633.5
73	424.5
74	308.0
75	207.0
76	133.0
77	60.5
78	30.0
79	14.0
80	8.5
81	5.0
82	2.5
83	1.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	8.280197237610278E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.280197237610278E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	8.280197237610278E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4154014E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.27007723666186
#Duplication Level	Percentage of total
1	21.517381186063304
2	9.482320307112097
3	6.022734151482389
4	4.266998226168376
5	3.0961188477576957
6	2.621012292874224
7	2.0772214973994143
8	1.7910555164429651
9	1.578625993617381
>10	19.200529788271183
>50	6.459358411373578
>100	12.380900356167238
>500	3.2908450167852967
>1k	4.909333035303773
>5k	0.6930388506351783
>10k+	0.6125265225457396
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	29158	0.12071699552712026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.815267060787495E-4	0.0	4.14009861880514E-6	0.0	0.001718140926804133	0.0
2	0.0013620924455868908	4.14009861880514E-6	4.14009861880514E-6	0.0	0.008383699703080408	0.0
3	0.0020038077315016876	4.14009861880514E-6	4.14009861880514E-6	0.0	0.012416155757796614	0.0
4	0.004835635186764403	4.14009861880514E-6	4.14009861880514E-6	0.0	0.01885814920865741	0.0
5	0.005067480709417491	4.14009861880514E-6	4.14009861880514E-6	0.0	0.022849204277185564	0.0
6	0.005978302405554622	4.14009861880514E-6	4.14009861880514E-6	0.0	0.029841830844347446	4.14009861880514E-6
7	0.007903448263299011	8.28019723761028E-6	4.14009861880514E-6	0.0	0.04040736251953816	4.14009861880514E-6
8	0.014606267927144532	8.28019723761028E-6	4.14009861880514E-6	0.0	0.05367637859280863	4.14009861880514E-6
9	0.015206582226871277	8.28019723761028E-6	4.14009861880514E-6	0.0	0.06277631535694232	4.14009861880514E-6
10-11	0.015864857907261297	8.28019723761028E-6	4.14009861880514E-6	0.0	0.10087350284718721	4.14009861880514E-6
12-13	0.016626636053121438	8.28019723761028E-6	4.14009861880514E-6	0.0	0.11780236609948144	4.14009861880514E-6
14-15	0.020056707758801497	1.656039447522056E-5	4.14009861880514E-6	8.28019723761028E-6	0.12579275643377535	4.14009861880514E-6
16-17	0.0236855041981842	1.656039447522056E-5	4.14009861880514E-6	8.28019723761028E-6	0.13186007095963428	4.14009861880514E-6
18-19	0.030108867205260376	1.656039447522056E-5	4.14009861880514E-6	8.28019723761028E-6	0.1385525403769328	6.21014792820771E-6
20-21	0.03530676102117023	2.6910641022233407E-5	4.14009861880514E-6	8.28019723761028E-6	0.1430569676741928	8.28019723761028E-6
22-23	0.03750101328913695	3.7260887569246255E-5	6.21014792820771E-6	8.28019723761028E-6	0.14736474028705954	8.28019723761028E-6
24-25	0.04308807637521449	4.76111341162591E-5	8.28019723761028E-6	8.28019723761028E-6	0.1515296794975775	8.28019723761028E-6
26-27	0.04821972861322346	6.624157790088224E-5	8.28019723761028E-6	8.28019723761028E-6	0.15457058193308987	8.28019723761028E-6
28-29	0.0608201187595569	6.624157790088224E-5	1.2420295856415418E-5	1.0350246547012848E-5	0.15738377894456798	8.28019723761028E-6
30-31	0.08440005044296157	6.624157790088224E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16056751478242912	8.28019723761028E-6
32-33	0.10239291904028872	7.245172582908993E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.1629522115868609	8.28019723761028E-6
34-35	0.11478009410775368	7.866187375729765E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16517751459446867	8.28019723761028E-6
36-37	0.11647339444284499	7.866187375729765E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16676731246408982	8.28019723761028E-6
38-39	0.11832194847614148	8.073192306670021E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16798450145801852	8.28019723761028E-6
40-41	0.11955983796316422	8.280197237610278E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16920997064918486	1.2420295856415418E-5
42-43	0.11983722457062415	8.280197237610278E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.1705555027002965	1.4490345165817988E-5
44-45	0.12068387473816981	8.487202168550536E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.1716753993766833	1.656039447522056E-5
46-47	0.12182861200626943	9.315221892311563E-5	1.2420295856415418E-5	1.656039447522056E-5	0.17274354482033505	1.656039447522056E-5
48-49	0.12288847725268355	1.0971261339833619E-4	1.2420295856415418E-5	1.656039447522056E-5	0.1736978375519696	1.656039447522056E-5
50-51	0.1237413375681574	1.159227613265439E-4	1.2420295856415418E-5	1.656039447522056E-5	0.1746417800370572	2.0700493094025696E-5
52-53	0.1246894201518638	1.221329092547516E-4	1.2420295856415418E-5	1.656039447522056E-5	0.17557330222628836	2.0700493094025696E-5
54-55	0.12515725129578878	1.3869330372997217E-4	1.2420295856415418E-5	1.656039447522056E-5	0.17643858283761862	2.0700493094025696E-5
56-57	0.12587348835684206	1.4076335303937475E-4	1.2420295856415418E-5	1.656039447522056E-5	0.17735147458306516	2.0700493094025696E-5
58-59	0.125991481167478	1.4283340234877732E-4	1.4490345165817988E-5	1.656039447522056E-5	0.17815258366580394	2.2770542403428264E-5
60-61	0.12629991851457897	1.4904355027698502E-4	1.656039447522056E-5	1.656039447522056E-5	0.17877980860655293	2.8980690331635975E-5
62-63	0.126639406601321	1.5939379682399787E-4	1.656039447522056E-5	1.656039447522056E-5	0.1795560770975789	2.8980690331635975E-5
64-65	0.12670771822853127	1.7181409268041326E-4	1.656039447522056E-5	1.8630443784623127E-5	0.18027024410932277	2.8980690331635975E-5
66-67	0.126798800398145	1.842343885368287E-4	1.656039447522056E-5	2.0700493094025696E-5	0.18086848835974012	2.8980690331635975E-5
68-69	0.12684848158157067	1.8630443784623126E-4	1.656039447522056E-5	2.0700493094025696E-5	0.1815102036456549	2.8980690331635975E-5
70-71	0.126958194194969	1.8837448715563384E-4	1.656039447522056E-5	2.0700493094025696E-5	0.18211258799469107	3.105073964103854E-5
72-73	0.12702443577286987	1.9458463508384156E-4	1.656039447522056E-5	2.4840591712830835E-5	0.18260732977963828	3.312078895044112E-5
74-75	0.12707411695629556	2.0079478301204926E-4	1.656039447522056E-5	2.4840591712830835E-5	0.18314761264939233	3.312078895044112E-5
76-77	0.1271030976466272	2.0286483232145183E-4	2.0700493094025696E-5	2.4840591712830835E-5	0.1838203786749482	3.312078895044112E-5
78-79	0.12714242858350583	2.0700493094025696E-4	2.0700493094025696E-5	2.4840591712830835E-5	0.1846939394835161	3.312078895044112E-5
80-81	0.1272107402107161	2.1321507886846468E-4	2.0700493094025696E-5	2.4840591712830835E-5	0.185449507481448	3.312078895044112E-5
82-83	0.12727077164068878	2.1528512817786726E-4	2.0700493094025696E-5	2.6910641022233407E-5	0.18628580740244666	3.933093687864882E-5
84-85	0.12731217262687683	2.1942522679667238E-4	2.0700493094025696E-5	2.8980690331635975E-5	0.18699790436488112	4.140098618805139E-5
86-87	0.12736806395823072	2.256353747248801E-4	2.6910641022233407E-5	2.8980690331635975E-5	0.18768930083422158	4.3471035497453966E-5
88-89	0.12740739489510935	2.2770542403428268E-4	2.8980690331635975E-5	2.8980690331635975E-5	0.18830824557773296	4.5541084806856534E-5
90-91	0.1274425857333692	2.3598562127189295E-4	2.8980690331635975E-5	2.8980690331635975E-5	0.18873674578477928	4.76111341162591E-5
92-93	0.12745707607853501	2.442658185095032E-4	2.8980690331635975E-5	2.8980690331635975E-5	0.18926253830936754	4.968118342566167E-5
94-95	0.12747570652231965	2.546160650565161E-4	2.8980690331635975E-5	3.105073964103854E-5	0.18984629221461907	5.589133135386938E-5
96-97	0.12749019686748547	2.6496631160352895E-4	3.312078895044112E-5	3.312078895044112E-5	0.19036587459127913	5.796138066327195E-5
98-99	0.1275150374591983	2.6496631160352895E-4	3.312078895044112E-5	3.312078895044112E-5	0.19079437479832545	6.624157790088224E-5
100-101	0.12753780800160172	2.7117645953173664E-4	3.5190838259843687E-5	3.312078895044112E-5	0.19124771559708462	7.452177513849251E-5
102-103	0.12755436839607692	2.794566567693469E-4	3.7260887569246255E-5	3.312078895044112E-5	0.19169070614929676	7.452177513849251E-5
104-105	0.12758127903709918	2.8980690331635974E-4	4.140098618805139E-5	3.312078895044112E-5	0.1921357667508183	7.866187375729765E-5
106-107	0.1276143998260496	2.8980690331635974E-4	4.140098618805139E-5	3.312078895044112E-5	0.19256633700717404	8.280197237610278E-5
108-109	0.1276785713546411	2.8980690331635974E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.19304658844695544	9.108216961371307E-5
110-111	0.12771997234082916	2.8980690331635974E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.19353097998535565	1.014324161607259E-4
112-113	0.1277510230804702	2.8980690331635974E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.19404642226339688	1.159227613265439E-4
114-115	0.12777793372149243	2.918769526257623E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.19444801182942098	1.2627300787355675E-4
116-117	0.12779863421458645	2.960170512445675E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.1948682318392297	1.3869330372997217E-4
118-119	0.1278214047569899	3.0636729779158034E-4	4.968118342566167E-5	3.7260887569246255E-5	0.19536090357486752	1.5525369820519272E-4
120-121	0.12784210525008388	3.1050739641038543E-4	5.3821282044466814E-5	4.140098618805139E-5	0.19595086762804725	1.5939379682399787E-4
122-123	0.12786280574317793	3.1878759364799573E-4	5.3821282044466814E-5	4.140098618805139E-5	0.1963835079337124	1.6146384613340044E-4
124-125	0.12787936613765316	3.208576429573983E-4	5.3821282044466814E-5	4.140098618805139E-5	0.19690102026106304	1.6353389544280302E-4
126-127	0.12791041687729418	3.229276922668009E-4	5.3821282044466814E-5	4.140098618805139E-5	0.19742681278565127	1.6560394475220556E-4
128-129	0.1279269772717694	3.249977415762035E-4	5.3821282044466814E-5	4.140098618805139E-5	0.19789464392957626	1.7181409268041326E-4
130-131	0.1279373275183164	3.3534798812321633E-4	5.589133135386938E-5	4.140098618805139E-5	0.19851772877170643	1.842343885368287E-4
132-133	0.12795595796210105	3.436281853608266E-4	6.003142997267452E-5	4.140098618805139E-5	0.19900419035941602	2.0286483232145183E-4
134-135	0.12798907875105148	3.436281853608266E-4	6.210147928207709E-5	4.140098618805139E-5	0.19943890071439058	2.111450295590621E-4
136-137	0.12800977924414553	3.456982346702292E-4	6.210147928207709E-5	4.3471035497453966E-5	0.19996469323897884	2.2356532541547753E-4
138-139	0.12802219954000194	3.539784319078394E-4	6.210147928207709E-5	4.5541084806856534E-5	0.2004780654677107	2.3391557196249038E-4
>>END_MODULE
