##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20724830
Total Bases	3.1 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.633346715027336	37.0	35.0	38.0	32.0	39.0
2	35.15361761712882	35.0	34.0	37.0	32.0	39.0
3	35.35019114752691	35.0	34.0	37.0	33.0	39.0
4	35.39950233608671	36.0	35.0	37.0	33.0	39.0
5	35.32232327116797	35.0	34.0	37.0	33.0	39.0
6	35.27182418384132	35.0	34.0	37.0	33.0	39.0
7	35.33483087677921	35.0	34.0	37.0	33.0	39.0
8	35.427821458607866	36.0	34.0	38.0	33.0	39.0
9	35.427311104602545	36.0	34.0	38.0	33.0	39.0
10-14	35.398019573622555	35.8	34.2	37.8	33.0	39.0
15-19	35.37243664724873	35.8	34.0	38.0	33.0	39.0
20-24	35.346298753717164	36.0	34.0	38.0	32.6	39.0
25-29	35.339340742481355	36.0	34.0	38.0	32.0	39.0
30-34	35.32726428154054	36.0	34.0	38.0	32.0	39.0
35-39	35.298716611909484	36.0	34.0	38.0	32.0	39.0
40-44	35.28881683468573	36.0	34.0	38.0	32.0	39.0
45-49	35.26293020497635	36.0	34.0	38.0	32.0	39.0
50-54	35.243733154867854	36.0	34.0	38.0	32.0	39.0
55-59	35.21831626121903	36.0	34.0	38.0	32.0	39.0
60-64	35.18029247043281	36.0	34.0	38.0	32.0	39.0
65-69	35.14502407016125	36.0	34.0	38.0	32.0	39.0
70-74	35.13096043731119	36.0	34.0	38.0	32.0	39.0
75-79	35.075498751980106	35.4	34.0	38.0	32.0	39.0
80-84	35.00818509005864	35.4	34.0	38.0	31.4	39.0
85-89	34.99445490264577	35.0	34.0	38.0	31.0	39.0
90-94	34.95077126326247	35.0	34.0	38.0	31.0	39.0
95-99	34.88650938029407	35.0	34.0	37.2	31.0	39.0
100-104	34.828687733506136	35.0	34.0	37.0	31.0	39.0
105-109	34.775775695144425	35.0	34.0	37.0	30.8	39.0
110-114	34.709668209582425	35.0	34.0	37.0	30.0	39.0
115-119	34.64726677130765	35.0	34.0	37.0	30.0	39.0
120-124	34.58042615548596	35.0	34.0	37.0	30.0	39.0
125-129	34.5053146201923	35.0	34.0	37.0	30.0	39.0
130-134	34.43185340482889	35.0	34.0	37.0	30.0	39.0
135-139	34.35644395635573	35.0	34.0	37.0	29.6	39.0
140-144	34.26733270188465	35.0	34.0	37.0	29.0	39.0
145-149	34.16997813733575	35.0	34.0	37.0	29.0	39.0
150	34.100108372420905	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	18.0
21	241.0
22	2129.0
23	9958.0
24	31475.0
25	74429.0
26	143315.0
27	243754.0
28	353242.0
29	461855.0
30	588665.0
31	756755.0
32	997792.0
33	1368626.0
34	2012884.0
35	3340152.0
36	8446821.0
37	1892714.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.579947338530644	19.234092631881662	12.370412688547988	34.81554734103971
2	23.79691528456037	26.992632846331325	33.362649216550835	15.847802652557471
3	18.45322253548039	30.36892461844078	29.07337237506894	22.104480471009893
4	22.4134721491081	35.08100187070292	21.64721737162621	20.858308608562773
5	24.67715296096518	35.4643488028611	21.906133850072592	17.952364386101117
6	18.449082573898075	39.004455042574534	22.494944469990827	20.051517913536564
7	19.877002609912843	20.70769699920337	37.00276431700525	22.41253607387853
8	20.736112190063803	23.39518345868217	28.701972464912863	27.166731886341168
9	22.87373165425241	23.604174316508267	28.185630473205332	25.336463556033994
10-14	22.875058565015973	28.820829893417706	25.165898103868646	23.13821343769768
15-19	22.845908989362034	27.873049863376444	26.63879703717714	22.642244110084377
20-24	22.836109770385477	27.689899075125023	26.615256548540454	22.858734605949042
25-29	22.84107420905262	27.29585429651293	26.642503702081033	23.22056779235342
30-34	22.78698568612612	26.961471114228402	26.805883829260686	23.445659370384796
35-39	23.06424419404164	26.67310853695784	26.893358353241016	23.3692889157595
40-44	23.28918982688881	26.208827768430424	26.8805592132722	23.621423191408567
45-49	23.148149345495234	26.0300065187507	26.95484402043346	23.867000115320607
50-54	23.4581253501235	26.06158120476742	26.62531562381935	23.854977821289726
55-59	23.467771750118093	26.133337643782845	26.582153870502196	23.816736735596866
60-64	23.188606130906745	26.07998618082754	26.66804022035404	24.06336746791168
65-69	23.43423034109327	25.986109415614024	26.649147906158944	23.930512337133766
70-74	23.613346888732018	25.967105158401782	26.33990339124615	24.079644561620047
75-79	23.427073708204123	26.111226002818842	26.602868153803914	23.85883213517312
80-84	23.74746285706352	26.119344832495873	26.281521545329912	23.851670765110693
85-89	23.476561660125785	26.21643750450908	26.488779430061033	23.8182214053041
90-94	23.451375958210512	25.961433700541814	26.559884930298583	24.027305410949086
95-99	23.533678428567843	26.0919739857164	26.558219551699285	23.816128034016472
100-104	23.749225445998835	25.96208991822852	26.501893622287852	23.78679101348479
105-109	23.53533708117268	26.145051129490565	26.356795206522804	23.962816582813947
110-114	23.640587642938446	26.265016407854734	26.258240960239483	23.83615498896734
115-119	23.833283071561986	26.361721664303158	26.414434280039934	23.390560984094925
120-124	23.78503466614684	26.568161958385183	26.500172015886257	23.14663135958172
125-129	23.730397788546394	26.609602105300745	26.37330873160359	23.286691374549275
130-134	23.893030726910666	26.652626824924496	26.369281678064425	23.08506077010041
135-139	23.627282092323863	26.60842406532091	26.696589807458874	23.067704034896344
140-144	23.71807923153049	26.80265459354793	26.421519501004354	23.05774667391723
145-149	23.856610663735864	27.266714790510214	26.03229978527674	22.84437476047718
150	23.6884355625595	27.264889506934438	25.98728192221601	23.059393008290055
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.5
2	8.5
3	18.0
4	26.0
5	23.5
6	17.5
7	14.5
8	10.5
9	8.5
10	6.5
11	5.0
12	6.0
13	10.0
14	15.0
15	22.5
16	37.0
17	84.5
18	169.0
19	255.0
20	366.0
21	551.5
22	861.5
23	1388.5
24	2250.0
25	3560.5
26	5536.0
27	8553.0
28	13014.5
29	18654.0
30	26422.5
31	38253.5
32	54787.5
33	75912.0
34	104354.0
35	149214.0
36	213102.5
37	289820.5
38	387308.0
39	507711.5
40	650725.0
41	806642.5
42	971393.5
43	1140043.5
44	1269562.5
45	1357819.0
46	1396062.5
47	1378398.0
48	1346852.5
49	1304726.5
50	1213854.5
51	1085072.0
52	935012.5
53	791199.5
54	677394.5
55	589137.0
56	486035.0
57	372373.0
58	303079.0
59	248106.5
60	184248.5
61	125146.0
62	78448.5
63	47905.0
64	29245.0
65	14494.0
66	7855.5
67	4446.5
68	2868.5
69	1859.5
70	823.5
71	566.5
72	357.5
73	258.0
74	210.0
75	132.5
76	78.0
77	25.0
78	8.5
79	0.5
80	0.5
81	0.5
82	0.0
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.825130049317654E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.024711421034575437
25-29	0.0
30-34	9.65026009863531E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.04774659189001791
85-89	1.930052019727062E-6
90-94	0.0
95-99	9.65026009863531E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	9.65026009863531E-7
140-144	0.0
145-149	0.02658453651972055
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.072483E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.37937523604751
#Duplication Level	Percentage of total
1	24.80267550879124
2	8.566841943079382
3	5.277874462992235
4	3.6487508498609578
5	2.7850482484385446
6	2.2276604684445607
7	1.844382231491631
8	1.5323375817930958
9	1.2997129568923103
>10	17.68928852980412
>50	6.232162867561898
>100	12.436620122848014
>500	3.8581105802983893
>1k	5.686943546536647
>5k	1.2307689328999825
>10k+	0.8808211682670649
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32998	0.15921964136738395	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	24288	0.11719275863782717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.846826729097416E-4	4.825130049317654E-6	0.0	0.0	0.0021857839123408974	0.0
2	0.0014137631044500727	1.4475390147952962E-5	0.0	0.0	0.010586335328202934	0.0
3	0.002258160863080662	1.4475390147952962E-5	0.0	0.0	0.015334263296731505	0.0
4	0.00555372468676462	1.4475390147952962E-5	0.0	0.0	0.023440481779585163	0.0
5	0.005741904758688008	1.4475390147952962E-5	0.0	0.0	0.028275262089001454	0.0
6	0.006851684670031069	1.4475390147952962E-5	0.0	0.0	0.03707147416890754	0.0
7	0.009273899954788532	1.4475390147952962E-5	0.0	0.0	0.04854563342618492	0.0
8	0.018649127640612734	1.4475390147952962E-5	0.0	0.0	0.06227795354654296	0.0
9	0.019435623838651512	1.9300520197270616E-5	0.0	0.0	0.07191856338507964	0.0
10-11	0.02024383312191222	1.9300520197270616E-5	0.0	0.0	0.11780072502404121	0.0
12-13	0.020989315714531796	1.9300520197270616E-5	0.0	0.0	0.13857049732132903	0.0
14-15	0.02428729210324041	1.9300520197270616E-5	0.0	0.0	0.15003983144855712	0.0
16-17	0.028053306106732845	1.9300520197270616E-5	0.0	4.825130049317654E-6	0.15826909074766837	0.0
18-19	0.03484226408612279	2.1713085221929442E-5	0.0	4.825130049317654E-6	0.16615335324825342	0.0
20-21	0.04022228409111196	2.412565024658827E-5	0.0	7.237695073976481E-6	0.17162022559413032	0.0
22-23	0.042526283689661146	2.8950780295905925E-5	4.825130049317654E-6	9.650260098635308E-6	0.17713776180552507	0.0
24-25	0.048082420941450425	3.136334532056475E-5	4.825130049317654E-6	9.650260098635308E-6	0.18239232842923198	0.0
26-27	0.05302576667697636	3.377591034522358E-5	4.825130049317654E-6	9.650260098635308E-6	0.18659501670218764	0.0
28-29	0.0663189999628465	3.377591034522358E-5	4.825130049317654E-6	9.650260098635308E-6	0.1906360631184912	0.0
30-31	0.09186082587890951	4.342617044385889E-5	9.650260098635308E-6	9.650260098635308E-6	0.1949497293825812	0.0
32-33	0.112123959521019	4.5838735468517716E-5	9.650260098635308E-6	9.650260098635308E-6	0.19794613514320744	0.0
34-35	0.12664036327439115	4.825130049317654E-5	9.650260098635308E-6	9.650260098635308E-6	0.20123687383684208	0.0
36-37	0.1287465325409183	4.825130049317654E-5	9.650260098635308E-6	9.650260098635308E-6	0.2032465405023829	0.0
38-39	0.13080686307197695	4.825130049317654E-5	9.650260098635308E-6	9.650260098635308E-6	0.20498599988516192	0.0
40-41	0.13224957695672293	4.825130049317654E-5	9.650260098635308E-6	9.650260098635308E-6	0.20680266134873	0.0
42-43	0.13260181145032313	4.825130049317654E-5	9.650260098635308E-6	9.650260098635308E-6	0.20842631761032537	0.0
44-45	0.13344862177397837	5.0663865517835366E-5	1.4475390147952962E-5	9.650260098635308E-6	0.20988350688521934	0.0
46-47	0.13429784466265826	6.755182069044716E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21154093905715993	0.0
48-49	0.1352701083675958	7.237695073976482E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21268449487884822	0.0
50-51	0.1363943636690868	7.720208078908247E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21404518155275581	0.0
52-53	0.13767543569718063	8.202721083840012E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21536485462124416	0.0
54-55	0.1383316533838878	8.202721083840012E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21629369215573782	0.0
56-57	0.13909884906172934	8.202721083840012E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21737210872176033	2.412565024658827E-6
58-59	0.13926049091838147	8.926490591237661E-5	1.688795517261179E-5	9.650260098635308E-6	0.2183467849917225	4.825130049317654E-6
60-61	0.1395934248917844	9.891516601101191E-5	1.9300520197270616E-5	9.650260098635308E-6	0.21905125397892286	1.4475390147952962E-5
62-63	0.1398805201297188	1.0132773103567073E-4	1.9300520197270616E-5	9.650260098635308E-6	0.2199752663833672	1.4475390147952962E-5
64-65	0.13994807195040923	1.0856542610964722E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22098130599864993	1.4475390147952962E-5
66-67	0.14000838607602573	1.1097799113430605E-4	1.9300520197270616E-5	9.650260098635308E-6	0.2217678021966887	1.4475390147952962E-5
68-69	0.14005904994154356	1.158031211836237E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22256153608980145	1.4475390147952962E-5
70-71	0.14017485306272717	1.158031211836237E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22331666894251967	1.4475390147952962E-5
72-73	0.14022792949326968	1.158031211836237E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22387155889819121	1.4475390147952962E-5
74-75	0.1402568802735656	1.2304081625760018E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22458326558046554	1.4475390147952962E-5
76-77	0.14029306874893543	1.326910763562355E-4	1.9300520197270616E-5	9.650260098635308E-6	0.2254011251238249	1.4475390147952962E-5
78-79	0.14033408235435466	1.495790315288473E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22646506629969942	1.4475390147952962E-5
80-81	0.14038233365484784	1.495790315288473E-4	1.9300520197270616E-5	1.4475390147952962E-5	0.2273118766233547	1.9300520197270616E-5
82-83	0.1404136970001684	1.519915965535061E-4	2.1713085221929442E-5	1.4475390147952962E-5	0.22819970055242914	1.9300520197270616E-5
84-85	0.14048124882085883	1.6164185665214141E-4	2.412565024658827E-5	1.4475390147952962E-5	0.22904168574603506	1.9300520197270616E-5
86-87	0.14053432525140136	1.688795517261179E-4	2.412565024658827E-5	1.4475390147952962E-5	0.2299126217199369	1.9300520197270616E-5
88-89	0.14058016398686984	1.7370468177543556E-4	2.412565024658827E-5	1.4475390147952962E-5	0.230802858214036	1.9300520197270616E-5
90-91	0.14061635246223975	1.785298118247532E-4	2.65382152712471E-5	1.4475390147952962E-5	0.23137704868990483	1.9300520197270616E-5
92-93	0.1406235901573137	1.785298118247532E-4	2.8950780295905925E-5	1.4475390147952962E-5	0.2318836873450832	1.9300520197270616E-5
94-95	0.14068631684795485	1.8335494187407086E-4	2.8950780295905925E-5	1.4475390147952962E-5	0.23256161811701231	1.9300520197270616E-5
96-97	0.14071044249820144	1.9059263694804735E-4	2.8950780295905925E-5	1.688795517261179E-5	0.233278149929336	2.1713085221929442E-5
98-99	0.14073698071347268	1.9300520197270616E-4	3.377591034522358E-5	1.9300520197270616E-5	0.2338499278401801	3.377591034522358E-5
100-101	0.14079005714401516	1.9300520197270616E-4	3.377591034522358E-5	1.9300520197270616E-5	0.23437104188550642	3.377591034522358E-5
102-103	0.14082383305436041	1.9300520197270616E-4	3.377591034522358E-5	2.1713085221929442E-5	0.23482219154511763	3.377591034522358E-5
104-105	0.14086243409475496	1.9300520197270616E-4	3.377591034522358E-5	2.65382152712471E-5	0.23536984380571518	3.377591034522358E-5
106-107	0.1408720843548536	1.9783033202202383E-4	3.377591034522358E-5	2.8950780295905925E-5	0.23593197145646067	3.377591034522358E-5
108-109	0.14090827283022345	2.147182871946356E-4	3.377591034522358E-5	2.8950780295905925E-5	0.23652546245252676	3.377591034522358E-5
110-111	0.1409709995208646	2.1713085221929443E-4	3.618847536988241E-5	2.8950780295905925E-5	0.23705381419292704	3.377591034522358E-5
112-113	0.14100718799623446	2.1713085221929443E-4	4.342617044385889E-5	2.8950780295905925E-5	0.2375604528481054	3.377591034522358E-5
114-115	0.14102166338638245	2.1954341724395325E-4	4.342617044385889E-5	2.8950780295905925E-5	0.23812499306387555	3.377591034522358E-5
116-117	0.141045789036629	2.2678111231792976E-4	4.342617044385889E-5	2.8950780295905925E-5	0.23856890502841277	3.377591034522358E-5
118-119	0.14106508955682628	2.2678111231792976E-4	4.342617044385889E-5	2.8950780295905925E-5	0.23914550806930623	3.860104039454123E-5
120-121	0.14110369059722083	2.2678111231792976E-4	4.342617044385889E-5	2.8950780295905925E-5	0.2397462367604463	4.1013605419200065E-5
122-123	0.14112540368244275	2.2678111231792976E-4	4.342617044385889E-5	2.8950780295905925E-5	0.24026493824074793	4.342617044385889E-5
124-125	0.1411398790725907	2.2678111231792976E-4	4.5838735468517716E-5	2.8950780295905925E-5	0.24080535280627152	4.342617044385889E-5
126-127	0.14116159215781265	2.2678111231792976E-4	5.30764305424942E-5	3.136334532056475E-5	0.24137230558706635	4.342617044385889E-5
128-129	0.1411881303730839	2.2678111231792976E-4	5.30764305424942E-5	3.377591034522358E-5	0.2420478237939708	4.342617044385889E-5
130-131	0.14120019319820717	2.2919367734258858E-4	5.30764305424942E-5	3.377591034522358E-5	0.24274746765112187	4.342617044385889E-5
132-133	0.14120743089328114	2.3401880739190622E-4	5.30764305424942E-5	3.377591034522358E-5	0.2433071827368427	4.342617044385889E-5
134-135	0.1412267314134784	2.4125650246588273E-4	5.30764305424942E-5	3.618847536988241E-5	0.24384277217231698	4.825130049317654E-5
136-137	0.14126050732382364	2.50906762564518E-4	5.5488995567153024E-5	3.860104039454123E-5	0.2444145500831611	4.825130049317654E-5
138-139	0.14127739527899624	2.50906762564518E-4	5.790156059181185E-5	3.860104039454123E-5	0.24508041802996694	4.825130049317654E-5
>>END_MODULE
