##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.S.3.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24153852
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.903927332170454	36.0	34.0	38.0	30.0	39.0
2	34.73530880291889	35.0	34.0	38.0	31.0	39.0
3	34.901324269106226	35.0	34.0	38.0	31.0	39.0
4	34.89042890550128	36.0	34.0	38.0	31.0	39.0
5	34.74523459032539	35.0	34.0	37.0	31.0	39.0
6	34.72914539676736	35.0	34.0	37.0	31.0	39.0
7	34.783654052363985	35.0	34.0	37.0	32.0	39.0
8	34.89139740526687	35.0	34.0	37.0	32.0	39.0
9	34.85514604461434	35.0	34.0	37.0	31.0	39.0
10-14	34.83627613516883	35.0	34.0	37.4	31.2	39.0
15-19	34.8519442364721	35.0	34.0	37.0	31.0	39.0
20-24	34.83906798799628	35.0	34.0	37.0	31.0	39.0
25-29	34.84288254312396	35.0	34.0	37.0	31.0	39.0
30-34	34.81866941968511	35.0	34.0	37.0	31.0	39.0
35-39	34.80898088636131	35.0	34.0	37.0	31.0	39.0
40-44	34.76274841793351	35.0	34.0	37.0	31.0	39.0
45-49	34.653726039225546	35.0	34.0	37.0	31.0	39.0
50-54	34.628717821074666	35.0	34.0	37.0	31.0	39.0
55-59	34.619480694011045	35.0	34.0	37.0	31.0	39.0
60-64	34.588963731333614	35.0	34.0	37.0	30.2	39.0
65-69	34.55838251389467	35.0	34.0	37.0	30.0	39.0
70-74	34.557099116116134	35.0	34.0	37.0	30.0	39.0
75-79	34.53925065037246	35.0	34.0	37.0	30.0	39.0
80-84	34.52149648014735	35.0	34.0	37.0	30.0	39.0
85-89	34.50533699552353	35.0	34.0	37.0	30.0	39.0
90-94	34.480783619937725	35.0	34.0	37.0	30.0	39.0
95-99	34.46001710203408	35.0	34.0	37.0	30.0	39.0
100-104	34.420445699509955	35.0	34.0	37.0	30.0	39.0
105-109	34.39073491052276	35.0	34.0	37.0	30.0	39.0
110-114	34.36239952120267	35.0	34.0	37.0	30.0	39.0
115-119	34.32291215496394	35.0	34.0	37.0	30.0	39.0
120-124	34.28162662419228	35.0	34.0	37.0	30.0	39.0
125-129	34.2619333346913	35.0	34.0	37.0	30.0	39.0
130-134	34.21507598042747	35.0	34.0	37.0	29.0	39.0
135-139	34.17382140952093	35.0	34.0	37.0	29.0	39.0
140-144	34.131486108302724	35.0	34.0	37.0	29.0	39.0
145-149	34.09454515991901	35.0	34.0	37.0	29.0	39.0
150	34.0930803086812	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	24.0
22	315.0
23	3335.0
24	21385.0
25	85046.0
26	213374.0
27	383853.0
28	564986.0
29	735982.0
30	924425.0
31	1164010.0
32	1487470.0
33	1950534.0
34	2681424.0
35	4163284.0
36	8287993.0
37	1486407.0
38	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.57431743715901	10.345477444147551	13.828384081689771	38.25182103700367
2	23.098818626622343	11.788441728740654	36.57742660099925	28.535313043637743
3	21.687729334710017	15.952570522237194	28.088827388389475	34.27087275466331
4	27.26181012542566	23.65980565648618	21.837556273168946	27.24082794491921
5	27.325408930021734	29.486896631328857	23.932062608546463	19.25563183010295
6	20.322242116010937	36.03826987906595	25.24768953788108	18.391798467042033
7	16.97731643856969	24.373785777703624	39.73813268162178	18.910765102104904
8	19.279039181499343	24.074959065486237	31.207266857921418	25.438734895093006
9	20.17238670380915	22.66033530493191	32.4626503410328	24.704627650226143
10-14	21.12105376580172	28.548923623594195	26.68072397189219	23.649298638711898
15-19	21.388011154490275	27.47603164830216	27.179625145845648	23.956332051361915
20-24	21.315543880427118	27.488190077721075	27.135045853072565	24.061220188779238
25-29	21.26174481328553	27.66380586807316	26.80401456528001	24.270434753361304
30-34	21.291745586332837	27.431632339466848	27.020177917486293	24.256444156714014
35-39	21.576621655898556	27.18667335281398	26.926623391855838	24.31008159943163
40-44	21.779891172830943	27.25741787065535	26.741961588909756	24.220729367603948
45-49	22.056403864467573	27.165128344576424	26.651412203940033	24.127055587015974
50-54	22.014966539628684	27.420094399618606	26.5638747821753	24.00106427857741
55-59	21.920887831208322	27.356141532979443	26.649911108871393	24.073059526940842
60-64	22.164941564770153	27.118043060982373	26.438670594360158	24.278344779887316
65-69	21.993814450276883	27.274542111013204	26.634153194060705	24.097490244649208
70-74	22.371150392501693	27.008105629197182	26.553224119961282	24.067519858339846
75-79	22.248517489085412	26.865635010891115	26.54893312356885	24.336914376454626
80-84	22.4815627120834	26.847745132990042	26.60991777715889	24.060774377767668
85-89	22.362488170096082	26.803858154012914	26.608443190647478	24.22521048524352
90-94	22.35447211940618	26.532541124015406	26.622127787464027	24.49085896911438
95-99	22.586169324152998	26.58838800356429	26.79407962653505	24.031363045747664
100-104	22.51905715975857	26.410462331791827	26.869598523470934	24.20088198497867
105-109	22.640981653311904	26.364027523120683	26.720666603328198	24.27432422023922
110-114	22.596157182142235	26.411152546440032	26.700545064515214	24.29214520690252
115-119	22.73988901689339	26.586737672193234	26.61714774590152	24.056225565011857
120-124	22.695690834809746	26.45950398622509	26.567264125557365	24.2775410534078
125-129	23.108715966533904	26.41277852251364	26.441336314205167	24.037169196747296
130-134	22.823301829415747	26.67722857480242	26.345168780722283	24.154300815059546
135-139	22.92358292316394	26.51910780901854	26.384990015253063	24.17231925256446
140-144	22.88862647467644	26.325680676010748	26.67590737784444	24.109785471468374
145-149	22.707311095834402	26.671975526340177	26.690576297412683	23.930137080412738
150	22.44961387685916	26.27362136420392	26.815846087801503	24.460918671135413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	3.5
12	8.5
13	11.0
14	18.0
15	38.5
16	87.5
17	215.5
18	507.5
19	892.5
20	1491.5
21	2415.5
22	3961.5
23	6534.0
24	10626.0
25	16389.5
26	23284.0
27	33266.0
28	51341.0
29	75963.0
30	103409.0
31	131141.0
32	158432.0
33	189909.0
34	224784.0
35	271321.5
36	336478.0
37	403751.5
38	470429.5
39	569966.5
40	707141.0
41	858137.5
42	1017164.5
43	1176706.5
44	1324738.0
45	1446534.5
46	1537485.5
47	1571700.0
48	1533455.5
49	1491522.5
50	1413536.0
51	1261837.5
52	1078756.0
53	918205.0
54	771818.5
55	638128.0
56	546415.5
57	455105.0
58	377325.0
59	308979.0
60	234089.5
61	159646.0
62	93022.5
63	54115.0
64	35510.5
65	22767.0
66	15040.5
67	7365.5
68	3136.0
69	2100.0
70	2367.5
71	1857.0
72	878.0
73	297.0
74	138.5
75	75.0
76	37.0
77	27.0
78	11.0
79	1.5
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.324840443669192E-4
2	6.210189579699337E-5
3	0.1411120677563148
4	9.522290688872317E-5
5	1.0350315966165563E-4
6	1.0764328604812185E-4
7	7.038214856992583E-5
8	1.2006366520752053E-4
9	7.038214856992583E-5
10-14	2.169426226508302E-4
15-19	2.6248401290195864E-4
20-24	3.908279308824117E-4
25-29	3.461145659085764E-4
30-34	2.93948973439102E-4
35-39	1.3165601908962595E-4
40-44	2.7904451844782357E-4
45-49	1.9044581377744633E-4
50-54	1.0598723549353536E-4
55-59	1.4738849935819762E-4
60-64	1.341400949215057E-4
65-69	0.05572278906072622
70-74	1.879617379455666E-4
75-79	1.3745219603067865E-4
80-84	1.1757958937564079E-4
85-89	8.694265411579072E-5
90-94	1.3910824658526516E-4
95-99	1.0101908382977589E-4
100-104	6.872609801533933E-5
105-109	8.777067939308397E-5
110-114	5.7961769410527155E-5
115-119	5.71337441332339E-5
120-124	9.439488161142993E-5
125-129	9.770698272060292E-5
130-134	3.8917188032782514E-5
135-139	4.140126386466225E-5
140-144	4.636941552842172E-5
145-149	6.044584524240688E-5
150	1.2006366520752053E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4153852E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.42151283420108
#Duplication Level	Percentage of total
1	34.41383083237371
2	8.299483899737634
3	4.91270079300224
4	3.2755772805510994
5	2.6236794404474075
6	2.0137506398762826
7	1.6933237899480007
8	1.4228803028243662
9	1.2188072197278605
>10	15.972099204660761
>50	5.34624442863239
>100	10.465614671133018
>500	3.598260504981933
>1k	4.07390152580306
>5k	0.5503609072573897
>10k+	0.119484559043025
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	4.140126386466225E-6	0.0	0.0	2.2770695125564236E-4	2.0700631932331125E-5
2	8.28025277293245E-6	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.0022729293861699575	2.484075831879735E-5
3	1.2420379159398675E-5	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.004214648661422617	5.796176941052715E-5
4	5.796176941052715E-5	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.0062060494533128716	8.28025277293245E-5
5	6.210189579699338E-5	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.007307323072112887	8.694265411579073E-5
6	8.694265411579073E-5	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.008984074258631708	9.108278050225695E-5
7	1.0350315966165563E-4	3.7261137478196024E-5	0.0	1.2420379159398675E-5	0.0106152840548994	9.522290688872318E-5
8	2.0286619293684503E-4	4.5541390251128474E-5	0.0	1.2420379159398675E-5	0.013898404279367118	9.93630332751894E-5
9	2.7738846789323706E-4	4.5541390251128474E-5	0.0	1.2420379159398675E-5	0.016862734772076934	9.93630332751894E-5
10-11	3.7675150116842646E-4	5.1751579830827815E-5	0.0	2.0700631932331125E-5	0.029719897265247798	1.2834391798045297E-4
12-13	4.3471327057895365E-4	5.3821643024060924E-5	0.0	2.0700631932331125E-5	0.03885508613698552	1.614649290721828E-4
14-15	4.7818459763684896E-4	6.210189579699338E-5	0.0	2.2770695125564237E-5	0.04615412895632548	1.7388530823158146E-4
16-17	5.299361774676768E-4	6.831208537669271E-5	0.0	2.484075831879735E-5	0.05070826798143832	1.7802543461804766E-4
18-19	6.210189579699337E-4	7.659233814962516E-5	0.0	2.484075831879735E-5	0.05398517801632634	1.821655610045139E-4
20-21	0.0010888532396406171	1.0557322285488873E-4	0.0	2.484075831879735E-5	0.05661001814534593	1.821655610045139E-4
22-23	0.0016146492907218278	1.0971334924135496E-4	0.0	2.484075831879735E-5	0.05921829776881965	1.8630568739098013E-4
24-25	0.0017968148517263417	1.159235388210543E-4	0.0	2.484075831879735E-5	0.06203358371161668	1.8837575058421323E-4
26-27	0.0018485664315571694	1.2006366520752053E-4	0.0	2.484075831879735E-5	0.06442864682618739	1.9251587697067946E-4
28-29	0.0019292988960932607	1.2420379159398676E-4	0.0	3.105094789849668E-5	0.0671797608099942	1.9458594016391256E-4
30-31	0.002136305215416572	1.2420379159398676E-4	0.0	4.3471327057895365E-5	0.0703676581275732	2.007961297436119E-4
32-33	0.0022149676167594304	1.2420379159398676E-4	0.0	6.0031832603760264E-5	0.07328644723003189	2.318470776421086E-4
34-35	0.00230812046045492	1.2420379159398676E-4	0.0	9.522290688872318E-5	0.07603963127703192	2.4219739360827416E-4
36-37	0.0023453815979331163	1.2834391798045297E-4	0.0	1.0350315966165563E-4	0.07853612748807105	2.504776463812066E-4
38-39	0.0023515917875128155	1.2834391798045297E-4	0.0	1.138534756278212E-4	0.08120029881776207	2.5461777276767283E-4
40-41	0.002380572672218079	1.345541075601523E-4	0.0	1.159235388210543E-4	0.08365539376493655	2.6289802554060524E-4
42-43	0.0023867828617977786	1.3869423394661856E-4	0.0	1.159235388210543E-4	0.08607529763782604	2.7738846789323706E-4
44-45	0.002415763746503042	1.4697448671955097E-4	0.0	1.159235388210543E-4	0.08864217599743511	2.815285942797033E-4
46-47	0.0024385344416286065	1.5318467629925033E-4	0.0	1.159235388210543E-4	0.09280921320541337	2.815285942797033E-4
48-49	0.0024550949471744713	1.5732480268571653E-4	0.0	1.159235388210543E-4	0.09536574124905625	2.856687206661695E-4
50-51	0.0024902860214594345	1.6146492907218276E-4	0.0	1.159235388210543E-4	0.09832179148899314	3.001591630188013E-4
52-53	0.00252754715893763	1.65605055458649E-4	0.0	1.159235388210543E-4	0.10073755523549618	3.0636935259850066E-4
54-55	0.0025710184859955255	1.65605055458649E-4	0.0	1.159235388210543E-4	0.10246191787545937	3.0636935259850066E-4
56-57	0.0026289802554060526	1.6974518184511523E-4	0.0	1.159235388210543E-4	0.10441812759306465	3.0636935259850066E-4
58-59	0.002689012088009813	1.8009549781128077E-4	0.0	1.159235388210543E-4	0.10645506977520605	3.0636935259850066E-4
60-61	0.002833916511536131	1.9044581377744636E-4	0.0	1.2006366520752053E-4	0.10822704386861359	3.1050947898496686E-4
62-63	0.0029167190392654554	1.9458594016391256E-4	0.0	1.2006366520752053E-4	0.11037990958957603	3.125795421782E-4
64-65	0.0029332795448113206	2.007961297436119E-4	0.0	1.2006366520752053E-4	0.1130606414248129	3.146496053714331E-4
66-67	0.002945699923970719	2.2563688806240926E-4	0.0	1.2006366520752053E-4	0.11532943068459639	3.208597949511324E-4
68-69	0.0029746808086759825	2.3805726722180796E-4	0.0	1.2213372840075363E-4	0.11721732831682499	3.31210110917298E-4
70-71	0.0030264323885068104	2.525477095744397E-4	0.0	1.2834391798045297E-4	0.11913420683375886	3.3742030049699735E-4
72-73	0.003036782704472976	2.608279623473722E-4	0.0	1.2834391798045297E-4	0.12070124467103632	3.3949036369023046E-4
74-75	0.0030657635891782396	2.608279623473722E-4	0.0	1.2834391798045297E-4	0.1222310213708356	3.3949036369023046E-4
76-77	0.00309267441069027	2.608279623473722E-4	0.0	1.366241707533854E-4	0.12357449238324389	3.3949036369023046E-4
78-79	0.0031402858641346315	2.608279623473722E-4	0.0	1.5318467629925033E-4	0.12490554301649277	3.3949036369023046E-4
80-81	0.0031568463696804968	2.670381519270715E-4	0.0	1.5318467629925033E-4	0.12625729428167398	3.3949036369023046E-4
82-83	0.0031796170648060607	2.7324834150677086E-4	0.0	1.5318467629925033E-4	0.12772289902248304	3.3949036369023046E-4
84-85	0.003237578834216588	2.7531840470000396E-4	0.0	1.5525473949248343E-4	0.12942449096732067	3.3949036369023046E-4
86-87	0.003281050161274483	2.8773878385940263E-4	0.0	1.635349922654159E-4	0.130821783622753	3.3949036369023046E-4
88-89	0.003295540603627115	2.8980884705263573E-4	0.0	1.676751186518821E-4	0.13224184697331093	3.3949036369023046E-4
90-91	0.0033038208564000475	2.93948973439102E-4	0.0	1.6974518184511523E-4	0.13402210131949138	3.3949036369023046E-4
92-93	0.0033183112987526793	2.93948973439102E-4	0.0	1.6974518184511523E-4	0.13583340661357035	3.3949036369023046E-4
94-95	0.003380413194549673	2.93948973439102E-4	0.0	1.7388530823158146E-4	0.13729901135437944	3.3949036369023046E-4
96-97	0.0034632157222789974	2.960190366323351E-4	0.0	1.7802543461804766E-4	0.13852655882796666	3.3949036369023046E-4
98-99	0.003537737997235389	3.0222922621203445E-4	0.0	1.8630568739098013E-4	0.13959471143567492	3.3949036369023046E-4
100-101	0.0036702220416023083	3.125795421782E-4	0.0	1.9044581377744636E-4	0.1406918449280885	3.3949036369023046E-4
102-103	0.003707483179080504	3.146496053714331E-4	0.0	1.9251587697067946E-4	0.1416978956399998	3.3949036369023046E-4
104-105	0.0037488844429451667	3.146496053714331E-4	0.0	2.007961297436119E-4	0.1426935960359449	3.3949036369023046E-4
106-107	0.0038130564019353934	3.208597949511324E-4	0.0	2.1114644570977746E-4	0.1438611116769284	3.3949036369023046E-4
108-109	0.0038399672234474234	3.2499992133759863E-4	0.0	2.1942669848270993E-4	0.1449127037790908	3.3949036369023046E-4
110-111	0.0038834385505053188	3.332801741105311E-4	0.0	2.1942669848270993E-4	0.14594152518612766	3.3949036369023046E-4
112-113	0.003986941710166975	3.394903636902304E-4	0.0	2.1942669848270993E-4	0.1469579262140051	3.3949036369023046E-4
114-115	0.004046973542770735	3.477706164631629E-4	0.0	2.2149676167594303E-4	0.14818961381397883	3.3949036369023046E-4
116-117	0.004131846133693292	3.6019099562256153E-4	0.0	2.2770695125564236E-4	0.1490445499127841	3.3949036369023046E-4
118-119	0.0041711773343647215	3.788215643616596E-4	0.0	2.318470776421086E-4	0.1498642949373044	3.4570055326992976E-4
120-121	0.004272610430833145	3.850317539413589E-4	0.0	2.463375199947404E-4	0.15071095078333674	3.477706164631629E-4
122-123	0.004512737761248185	3.850317539413589E-4	0.0	2.649680887338384E-4	0.15147687416483302	3.477706164631629E-4
124-125	0.004647291868808337	3.850317539413589E-4	0.0	2.649680887338384E-4	0.15215999501859995	3.477706164631629E-4
126-127	0.004684553006286533	3.933120067142914E-4	0.0	2.649680887338384E-4	0.15290935789455032	3.477706164631629E-4
128-129	0.0047321644597308945	3.933120067142914E-4	2.0700631932331125E-6	2.691082151203046E-4	0.1535779883059646	3.477706164631629E-4
130-131	0.004895699451996311	3.933120067142914E-4	4.140126386466225E-6	2.7324834150677086E-4	0.15423626840141275	3.477706164631629E-4
132-133	0.004997132548464733	3.933120067142914E-4	4.140126386466225E-6	2.877387838594027E-4	0.15489868862324735	3.477706164631629E-4
134-135	0.005102705771319622	3.933120067142914E-4	4.140126386466225E-6	2.93948973439102E-4	0.15569152282625562	3.477706164631629E-4
136-137	0.005264170700391806	4.0573238587369005E-4	4.140126386466225E-6	2.960190366323351E-4	0.15637671374321577	3.477706164631629E-4
138-139	0.005373884049633159	4.098725122601563E-4	4.140126386466225E-6	3.001591630188013E-4	0.15705155434420975	3.477706164631629E-4
>>END_MODULE
