##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.S.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24153852
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.61724585378763	37.0	35.0	38.0	32.0	39.0
2	35.24060841310115	35.0	34.0	37.0	32.0	39.0
3	35.39319744941718	35.0	34.0	37.0	33.0	39.0
4	35.45134237801904	36.0	35.0	38.0	33.0	39.0
5	35.36987466843798	36.0	34.0	37.0	33.0	39.0
6	35.32585597527053	35.0	34.0	37.0	33.0	39.0
7	35.385852161386104	35.0	34.0	37.0	33.0	39.0
8	35.476938916409686	36.0	34.0	38.0	33.0	39.0
9	35.46931325901972	36.0	34.0	38.0	33.0	39.0
10-14	35.434008538265445	35.8	34.4	37.8	33.0	39.0
15-19	35.40741086763304	36.0	34.0	38.0	33.0	39.0
20-24	35.37927786425122	36.0	34.0	38.0	33.0	39.0
25-29	35.370112278571554	36.0	34.0	38.0	33.0	39.0
30-34	35.35347119788595	36.0	34.0	38.0	32.8	39.0
35-39	35.32532864737268	36.0	34.0	38.0	32.0	39.0
40-44	35.30677834740397	36.0	34.0	38.0	32.0	39.0
45-49	35.278993271963415	36.0	34.0	38.0	32.0	39.0
50-54	35.25782692549412	36.0	34.0	38.0	32.0	39.0
55-59	35.22615362551696	36.0	34.0	38.0	32.0	39.0
60-64	35.184183185356936	36.0	34.0	38.0	32.0	39.0
65-69	35.14640999704726	36.0	34.0	38.0	32.0	39.0
70-74	35.12772267545565	36.0	34.0	38.0	32.0	39.0
75-79	35.07000770725928	35.4	34.0	37.6	32.0	39.0
80-84	34.994571259275745	35.4	34.0	37.2	31.6	39.0
85-89	34.97522761172835	35.0	34.0	37.0	31.2	39.0
90-94	34.92679917058364	35.0	34.0	37.0	31.0	39.0
95-99	34.860003000763605	35.0	34.0	37.0	31.0	39.0
100-104	34.793495637880035	35.0	34.0	37.0	31.0	39.0
105-109	34.73782689402916	35.0	34.0	37.0	30.8	39.0
110-114	34.663246226730216	35.0	34.0	37.0	30.0	39.0
115-119	34.59723035481049	35.0	34.0	37.0	30.0	39.0
120-124	34.524005131769464	35.0	34.0	37.0	30.0	39.0
125-129	34.44094461620449	35.0	34.0	37.0	30.0	39.0
130-134	34.36094863875129	35.0	34.0	37.0	29.8	39.0
135-139	34.27299956959246	35.0	34.0	37.0	29.0	39.0
140-144	34.17223345576515	35.0	34.0	37.0	29.0	39.0
145-149	34.06359923874668	35.0	34.0	37.0	28.8	39.0
150	33.99223664200642	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	11.0
21	259.0
22	2134.0
23	10375.0
24	34005.0
25	81091.0
26	158650.0
27	270246.0
28	395613.0
29	525295.0
30	680817.0
31	889684.0
32	1188525.0
33	1646747.0
34	2443318.0
35	4086978.0
36	9757348.0
37	1982748.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.97772471147562	18.847847492310855	14.381035968136096	32.79339182807743
2	24.067610416756715	26.085027762859525	32.28968613370654	17.55767568667722
3	19.622712766477164	29.422996381695143	29.5466909377436	21.407599914084095
4	23.222705844185846	34.2014267537948	21.445378567360603	21.130488834658752
5	25.249844207044074	35.062101067771714	21.54310211058675	18.144952614597457
6	19.12183199598971	39.15076568325417	21.700903027806913	20.026499292949214
7	20.567816677853287	20.876827431086355	36.28310714166834	22.272248749392023
8	21.534610711368106	23.555812132988148	27.96257921924834	26.946997936395405
9	23.44775069417499	23.59431530838228	27.530321043616564	25.427612953826163
10-14	23.062849131168388	28.791849256937148	24.989878425932876	23.155423185961588
15-19	23.12906198150092	27.695434252060497	26.473276395003165	22.702227371435413
20-24	23.05662964855823	27.62160939286048	26.413118101263315	22.908642857317975
25-29	23.144372168878075	27.23103710331586	26.400892081312744	23.22369864649332
30-34	22.99255042218525	26.97041117913615	26.59383273525068	23.44320566342793
35-39	23.3062767793725	26.695045576995334	26.65925335635906	23.339424287273104
40-44	23.52944449605802	26.330309550625714	26.66571692167361	23.474529031642653
45-49	23.421435222837335	26.160444305115394	26.742119642034734	23.67600083001254
50-54	23.64918956735505	26.19450616981926	26.441983054644723	23.714321208180966
55-59	23.690030890310997	26.21861887702218	26.453136336183565	23.638213896483258
60-64	23.387723829722894	26.193612513647928	26.52780848371514	23.890855172914037
65-69	23.625597275333142	26.1031739368114	26.53428612545941	23.736942662396043
70-74	23.75972163777438	26.07389827510742	26.236887598715104	23.929492488403092
75-79	23.569323849463018	26.197480219718162	26.517410142282895	23.71578578853592
80-84	23.92177213448361	26.212562561446713	26.175869451034895	23.689795853034784
85-89	23.6472958735302	26.310222703279152	26.418805798916097	23.62367562427455
90-94	23.660521725478816	26.07731305135098	26.46229015562404	23.79987506754616
95-99	23.65340236414465	26.256545746823324	26.471222064290203	23.618829824741827
100-104	23.889143644665868	26.064231079995025	26.428226023741473	23.618399251597634
105-109	23.69947865872491	26.228902950966166	26.339792096101277	23.731826294207647
110-114	23.772084883189645	26.364492090122933	26.22937823747533	23.634044789212087
115-119	23.933168092608998	26.430188443648657	26.37354240640375	23.2631010573386
120-124	23.904771793749504	26.577909809168325	26.509917341548668	23.007401055533503
125-129	23.832720346220555	26.660787687197885	26.361743046202317	23.144748920379243
130-134	23.988230117498443	26.678177874071597	26.37531686457299	22.95827514385697
135-139	23.779558639342497	26.64740265859044	26.684917171803484	22.88812153026358
140-144	23.7913770441253	26.80621211059834	26.476550406949585	22.925860438326772
145-149	23.950349845353276	27.31785234676306	26.03615632015252	22.695641487731137
150	23.72209202904779	27.36739050980357	25.971331612034387	22.939185849114253
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	8.5
3	21.5
4	28.0
5	28.5
6	22.0
7	17.0
8	17.5
9	12.0
10	9.5
11	8.0
12	6.0
13	8.0
14	14.0
15	28.0
16	57.0
17	105.5
18	178.5
19	283.0
20	455.0
21	669.5
22	1014.0
23	1658.0
24	2717.5
25	4291.0
26	6546.5
27	10008.5
28	15165.5
29	21482.5
30	29920.0
31	42721.0
32	60988.5
33	84199.0
34	115477.0
35	165137.0
36	236378.5
37	324479.5
38	434560.0
39	570170.5
40	733179.5
41	914205.5
42	1108511.0
43	1307209.0
44	1465670.0
45	1574267.0
46	1625500.5
47	1612116.5
48	1577242.5
49	1538806.5
50	1438976.0
51	1281593.0
52	1102376.0
53	932112.0
54	795261.5
55	680864.0
56	565498.0
57	453577.0
58	374224.5
59	307133.0
60	232100.5
61	158399.5
62	99058.0
63	59919.5
64	37178.0
65	19551.5
66	10772.5
67	6214.0
68	4018.5
69	2773.0
70	1843.0
71	1607.5
72	1015.0
73	753.5
74	654.5
75	408.0
76	264.5
77	85.0
78	16.5
79	1.5
80	1.5
81	0.5
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.140126386466225E-6
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	8.28025277293245E-7
15-19	0.0
20-24	0.02884508855978748
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.28025277293245E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.056410878066156904
85-89	8.28025277293245E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.030092922652668403
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4153852E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.02942444792733
#Duplication Level	Percentage of total
1	24.721171457288627
2	8.140678743207589
3	5.112319980378326
4	3.6604353587232783
5	2.7780846510213637
6	2.2351003202781405
7	1.7997952683789946
8	1.5921142085220399
9	1.334594463946579
>10	17.424943137932193
>50	6.16084409387316
>100	12.960553795212219
>500	4.179745749396133
>1k	5.964990463906049
>5k	1.201785281137126
>10k+	0.7328430267981727
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32247	0.13350665558437635	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.5668783596090593E-4	0.0	0.0	0.0	0.00246751532633387	0.0
2	0.0014449041088767124	4.140126386466225E-6	0.0	0.0	0.010644264939604664	0.0
3	0.002136305215416572	1.2420379159398675E-5	0.0	0.0	0.014709869051114497	0.0
4	0.004939170779054206	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.021557638094329635	0.0
5	0.0050675146970346596	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.02537897474903796	0.0
6	0.006057004903400087	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.03526973668630577	0.0
7	0.008205730497976058	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.046630243490769094	0.0
8	0.015285346618833302	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.06215571744001744	0.0
9	0.015807002543528046	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.07284552376987323	0.0
10-11	0.016465282638976175	1.65605055458649E-5	4.140126386466225E-6	0.0	0.12595299499226872	0.0
12-13	0.01718773469341453	1.65605055458649E-5	4.140126386466225E-6	0.0	0.14833658830069837	0.0
14-15	0.019928498361255174	1.65605055458649E-5	4.140126386466225E-6	0.0	0.1599289421828038	0.0
16-17	0.023161937069085294	1.8630568739098012E-5	6.210189579699337E-6	6.210189579699337E-6	0.16773308042129265	0.0
18-19	0.029129929255176358	2.6910821512030462E-5	8.28025277293245E-6	8.28025277293245E-6	0.17514390665306717	0.0
20-21	0.034435501219432826	2.8980884705263574E-5	8.28025277293245E-6	8.28025277293245E-6	0.18003546597867703	0.0
22-23	0.036594577129974964	3.31210110917298E-5	8.28025277293245E-6	8.28025277293245E-6	0.18520027364579364	0.0
24-25	0.04165588163742992	3.5191074284962915E-5	8.28025277293245E-6	8.28025277293245E-6	0.18999039987493505	0.0
26-27	0.04604855573347059	3.7261137478196024E-5	8.28025277293245E-6	8.28025277293245E-6	0.19345154553402083	0.0
28-29	0.056032470514433885	3.7261137478196024E-5	1.0350315966165562E-5	8.28025277293245E-6	0.19692718163545925	0.0
30-31	0.07837673262219211	4.140126386466225E-5	1.2420379159398675E-5	8.28025277293245E-6	0.2007837093644525	0.0
32-33	0.09829281060428788	4.96815166375947E-5	1.2420379159398675E-5	8.28025277293245E-6	0.20373768954119617	0.0
34-35	0.11267353960767831	5.3821643024060924E-5	1.2420379159398675E-5	8.28025277293245E-6	0.20661921750617665	0.0
36-37	0.11510793392292046	5.3821643024060924E-5	1.65605055458649E-5	8.28025277293245E-6	0.2087410322792406	0.0
38-39	0.11738707349867011	5.3821643024060924E-5	1.65605055458649E-5	8.28025277293245E-6	0.2103246306220639	0.0
40-41	0.11874710501662426	5.589170621729403E-5	1.65605055458649E-5	8.28025277293245E-6	0.21195791048152485	0.0
42-43	0.11896446165191374	6.210189579699338E-5	1.65605055458649E-5	8.28025277293245E-6	0.2138561584297196	0.0
44-45	0.11974487547576262	6.62420221834596E-5	1.65605055458649E-5	8.28025277293245E-6	0.21528864215943694	0.0
46-47	0.12088962042162053	7.866240134285827E-5	1.65605055458649E-5	8.28025277293245E-6	0.21693434239805726	0.0
48-49	0.12203643543067168	8.694265411579073E-5	1.65605055458649E-5	8.28025277293245E-6	0.21816395993483773	0.0
50-51	0.12292863266695514	8.694265411579073E-5	1.65605055458649E-5	8.28025277293245E-6	0.2194618895569949	0.0
52-53	0.12390156236777472	8.901271730902383E-5	1.65605055458649E-5	8.28025277293245E-6	0.22063975551394455	2.0700631932331125E-6
54-55	0.12441700810288975	9.315284369549006E-5	1.65605055458649E-5	8.28025277293245E-6	0.22161475527795732	4.140126386466225E-6
56-57	0.1252698741385018	9.72929700819563E-5	1.8630568739098012E-5	8.28025277293245E-6	0.2226704875065062	4.140126386466225E-6
58-59	0.12543961932034692	1.0557322285488873E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22366204777606485	4.140126386466225E-6
60-61	0.1257149377250469	1.138534756278212E-4	2.0700631932331125E-5	8.28025277293245E-6	0.224409340588822	6.210189579699337E-6
62-63	0.12599232619294015	1.159235388210543E-4	2.0700631932331125E-5	8.28025277293245E-6	0.2253698499104822	8.28025277293245E-6
64-65	0.1260378675831913	1.28343917980453E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22633035923214234	8.28025277293245E-6
66-67	0.12614551086923942	1.4283436033308476E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22692860749498672	8.28025277293245E-6
68-69	0.12621589301780933	1.4490442352631786E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22766762005497093	1.0350315966165562E-5
70-71	0.12632146624066423	1.5111461310601723E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22849150520587772	1.2420379159398675E-5
72-73	0.12639598851562062	1.6146492907218276E-4	2.0700631932331125E-5	8.28025277293245E-6	0.229098033721495	1.2420379159398675E-5
74-75	0.1264125490211665	1.65605055458649E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22973561318501085	2.0700631932331125E-5
76-77	0.12643738977948527	1.7595537142481456E-4	2.0700631932331125E-5	8.28025277293245E-6	0.23050153656650707	2.0700631932331125E-5
78-79	0.12650984199124843	1.9251587697067946E-4	2.0700631932331125E-5	8.28025277293245E-6	0.23160695031169354	2.484075831879735E-5
80-81	0.12656780376065896	1.9458594016391256E-4	2.0700631932331125E-5	1.2420379159398675E-5	0.23246809660007853	2.8980884705263574E-5
82-83	0.12661748527729655	1.987260665503788E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23323401998157478	2.8980884705263574E-5
84-85	0.12666716679393414	2.0700631932331126E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23415519810256352	2.8980884705263574E-5
86-87	0.12671477824737853	2.0700631932331126E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23496873293750414	2.8980884705263574E-5
88-89	0.12675203938485669	2.0700631932331126E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23579468815160415	3.105094789849668E-5
90-91	0.12676652982720935	2.1114644570977746E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23639500647764172	3.31210110917298E-5
92-93	0.1267768801431755	2.1114644570977746E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23693529297107557	3.31210110917298E-5
94-95	0.126807931091074	2.1321650890301057E-4	2.0700631932331125E-5	1.65605055458649E-5	0.2376722354678666	3.5191074284962915E-5
96-97	0.12682242153342665	2.1942669848270993E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23830981493138237	3.7261137478196024E-5
98-99	0.12684933235493867	2.1942669848270993E-4	2.0700631932331125E-5	1.65605055458649E-5	0.2388190504769177	4.140126386466225E-5
100-101	0.12689073361880332	2.2149676167594303E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23941936880295533	4.140126386466225E-5
102-103	0.12690315399796273	2.2356682486917616E-4	2.484075831879735E-5	1.65605055458649E-5	0.2399741457387418	4.140126386466225E-5
104-105	0.12692592469308828	2.2563688806240926E-4	2.484075831879735E-5	1.65605055458649E-5	0.2405434131168809	4.761145344436158E-5
106-107	0.12694455526182738	2.318470776421086E-4	2.484075831879735E-5	1.65605055458649E-5	0.2410692091679621	4.96815166375947E-5
108-109	0.12701286734720407	2.3598720402857483E-4	2.8980884705263574E-5	1.65605055458649E-5	0.24155360395517866	5.3821643024060924E-5
110-111	0.12707910936938754	2.3598720402857483E-4	3.31210110917298E-5	1.65605055458649E-5	0.24209389044861251	6.210189579699338E-5
112-113	0.1271080902540928	2.3598720402857483E-4	3.31210110917298E-5	1.65605055458649E-5	0.2427004189642298	6.210189579699338E-5
114-115	0.12711844057005897	2.3598720402857483E-4	3.31210110917298E-5	1.65605055458649E-5	0.2432676162791757	6.41719589902265E-5
116-117	0.12713086094921838	2.3598720402857483E-4	3.31210110917298E-5	1.65605055458649E-5	0.24375201106639222	7.038214856992583E-5
118-119	0.1271391412019913	2.3598720402857483E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24428401730705313	7.452227495639205E-5
120-121	0.12714535139157102	2.3598720402857483E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24494850759208098	7.659233814962516E-5
122-123	0.12716191189711684	2.4012733041504106E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24551156478064037	7.866240134285828E-5
124-125	0.1271764023394695	2.4426745680150726E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24607048184281333	8.28025277293245E-5
126-127	0.1271908927818221	2.484075831879735E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24671220143271558	8.28025277293245E-5
128-129	0.12719710297140183	2.504776463812066E-4	3.7261137478196024E-5	1.65605055458649E-5	0.2473580611490043	8.901271730902383E-5
130-131	0.12720952335056124	2.5875789915413903E-4	3.7261137478196024E-5	1.65605055458649E-5	0.2480887934562156	9.108278050225695E-5
132-133	0.1272260838561071	2.608279623473722E-4	3.7261137478196024E-5	1.65605055458649E-5	0.2487470735516637	9.522290688872318E-5
134-135	0.12723436410888003	2.649680887338384E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24926665941316523	9.522290688872318E-5
136-137	0.12724678448803942	2.649680887338384E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24989802868710134	9.72929700819563E-5
138-139	0.12727369530955146	2.670381519270715E-4	3.7261137478196024E-5	1.65605055458649E-5	0.25056251897212917	1.0143309646842251E-4
>>END_MODULE
