##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.R.1.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24134818
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.90373973402244	36.0	34.0	38.0	30.0	39.0
2	34.759031785530766	35.0	34.0	38.0	31.0	39.0
3	34.95337934597228	35.0	34.0	38.0	31.0	39.0
4	34.88935006677904	36.0	34.0	38.0	31.0	39.0
5	34.75311696984829	35.0	34.0	37.0	31.0	39.0
6	34.744326930495184	35.0	34.0	37.0	31.0	39.0
7	34.75074338658779	35.0	34.0	37.0	32.0	39.0
8	34.895486553907304	35.0	34.0	37.0	32.0	39.0
9	34.88432765476002	35.0	34.0	37.0	32.0	39.0
10-14	34.86051425786596	35.0	34.0	37.0	31.4	39.0
15-19	34.85570103739751	35.0	34.0	37.0	31.0	39.0
20-24	34.865495758037206	35.0	34.0	37.0	31.0	39.0
25-29	34.84805426749023	35.0	34.0	37.0	31.0	39.0
30-34	34.838304759538694	35.0	34.0	37.0	31.0	39.0
35-39	34.83385223787476	35.0	34.0	37.0	31.0	39.0
40-44	34.76981748940473	35.0	34.0	37.0	31.0	39.0
45-49	34.67647781723484	35.0	34.0	37.0	31.0	39.0
50-54	34.63764096335842	35.0	34.0	37.0	31.0	39.0
55-59	34.62288182989405	35.0	34.0	37.0	31.0	39.0
60-64	34.59763151311106	35.0	34.0	37.0	31.0	39.0
65-69	34.57633236761926	35.0	34.0	37.0	30.4	39.0
70-74	34.54819036961455	35.0	34.0	37.0	30.0	39.0
75-79	34.5229941572379	35.0	34.0	37.0	30.0	39.0
80-84	34.50068390820267	35.0	34.0	37.0	30.0	39.0
85-89	34.47620393905601	35.0	34.0	37.0	30.0	39.0
90-94	34.450194768404714	35.0	34.0	37.0	30.0	39.0
95-99	34.418053386605195	35.0	34.0	37.0	30.0	39.0
100-104	34.37964364181242	35.0	34.0	37.0	30.0	39.0
105-109	34.32506169302789	35.0	34.0	37.0	30.0	39.0
110-114	34.28779518453381	35.0	34.0	37.0	30.0	39.0
115-119	34.23634433870601	35.0	34.0	37.0	30.0	39.0
120-124	34.20240947331776	35.0	34.0	37.0	29.6	39.0
125-129	34.15203459168409	35.0	34.0	37.0	29.0	39.0
130-134	34.09538794947615	35.0	34.0	37.0	29.0	39.0
135-139	34.048691761421196	35.0	34.0	37.0	29.0	39.0
140-144	34.01724562414351	35.0	34.0	37.0	29.0	39.0
145-149	33.95324979869332	35.0	34.0	37.0	29.0	39.0
150	33.915155399141604	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	27.0
22	420.0
23	4201.0
24	25225.0
25	93658.0
26	224232.0
27	393290.0
28	568289.0
29	733638.0
30	917686.0
31	1153032.0
32	1475774.0
33	1953087.0
34	2714534.0
35	4248572.0
36	8589499.0
37	1039647.0
38	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.000413843247216	10.506882567876632	14.647904667884992	38.84479892099116
2	22.53149522191467	12.244503490648265	36.775632270319356	28.44836901711771
3	21.0262717353703	16.919785983698144	28.54871743897411	33.50522484195744
4	26.24201024205499	24.725905181334593	22.332482073089054	26.69960250352137
5	25.99990851387755	30.53114406379367	24.4161461736095	19.052801248719277
6	19.273076312245017	36.69311906129916	25.485337502029747	18.548467124426082
7	15.92307017252368	24.930413684420664	40.574442503584244	18.572073639471412
8	18.349461427090283	24.328346440478214	31.67379337414052	25.648398758290984
9	19.472874002389663	22.75518847287686	33.02740992373709	24.74452760099639
10-14	20.16922746177958	29.269307774912377	27.167759116255723	23.393705647052318
15-19	20.354157658395966	27.86176906447162	27.970455432726105	23.81361784440631
20-24	20.521572221353996	28.04606760238813	27.677560328241057	23.754799848016813
25-29	20.51503944337587	28.23249593606722	27.558336880362745	23.694127740194165
30-34	20.46828792926771	27.97864124721497	27.58907737480063	23.963993448716693
35-39	20.57598748593847	27.956864196173047	27.610287691687528	23.85686062620095
40-44	20.605210338039413	28.055640166800437	27.520707790187355	23.81844170497279
45-49	20.70845958804837	27.84528751162511	27.464881947834403	23.981370952492117
50-54	20.809847113058098	27.814698778029985	27.569358915736032	23.80609519317589
55-59	20.816364886692202	27.859489888168294	27.4867443193003	23.837400905839203
60-64	20.762439267145126	27.77238771749973	27.4697519392139	23.995421076141245
65-69	20.857622627895616	27.71991711035971	27.542069749163666	23.880390512581013
70-74	20.975236837347104	27.64483256123846	27.531800420275708	23.84813018113873
75-79	20.97452047396108	27.549385599396643	27.541755110685134	23.934338815957148
80-84	21.029898208766213	27.56071202261708	27.49837866563044	23.911011102986265
85-89	21.005285736367707	27.56996431142442	27.571663108405183	23.853086843802686
90-94	21.006078610445737	27.46400887312317	27.511010027443277	24.018902488987816
95-99	21.08128893666253	27.379048772539942	27.631298427959898	23.90836386283762
100-104	21.148660023427666	27.43376728780657	27.485969275872474	23.93160341289329
105-109	21.092911129153148	27.268289989108318	27.62347660940268	24.015322272335858
110-114	21.202695123748953	27.24194933098605	27.645967466125263	23.909388079139738
115-119	21.233010986916888	27.277555088822552	27.607201626261883	23.882232297998673
120-124	21.17812849480382	27.212758315603857	27.595057545135028	24.0140556444573
125-129	21.21656742966207	27.120586296110766	27.680467084917275	23.98237918930989
130-134	21.294679419669723	27.22723381850644	27.570587258037165	23.907499503786667
135-139	21.28920389509634	27.07568297939	27.66542864954466	23.969684475969004
140-144	21.342216856208623	27.0154072589915	27.784426918901715	23.85794896589816
145-149	21.356520147488762	27.543815553181094	27.465279911915978	23.63438438741417
150	21.246955169260666	27.09352325362132	27.560165615091957	24.099355962026053
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	3.0
9	4.5
10	5.5
11	5.5
12	7.5
13	15.0
14	35.0
15	73.5
16	158.5
17	339.5
18	663.5
19	1235.5
20	2304.0
21	3868.0
22	6065.5
23	9660.5
24	15062.0
25	23290.0
26	34507.5
27	48320.0
28	65385.0
29	91025.0
30	123785.0
31	157983.0
32	189855.0
33	224776.0
34	264414.5
35	312188.0
36	386008.0
37	485485.0
38	603019.5
39	749435.0
40	918767.0
41	1083734.5
42	1252699.0
43	1429725.5
44	1573900.0
45	1659156.5
46	1682315.0
47	1643495.5
48	1555846.0
49	1428488.0
50	1262578.5
51	1081788.0
52	900033.0
53	720599.5
54	557618.5
55	421294.5
56	316418.5
57	236705.0
58	177260.5
59	130951.5
60	91237.5
61	63691.5
62	45332.5
63	30033.5
64	20032.0
65	14875.0
66	13229.5
67	9658.5
68	4911.5
69	3077.5
70	2879.0
71	2002.0
72	869.5
73	303.5
74	138.0
75	91.5
76	50.5
77	30.5
78	12.0
79	1.5
80	1.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.148977547707217E-4
2	1.9059600946648946E-4
3	8.701122171296258E-5
4	5.3864089631833976E-5
5	4.143391510141075E-5
6	6.215087265211613E-5
7	1.8230922644620732E-4
8	7.043765567239827E-5
9	7.872443869268042E-5
10-14	1.4667605945899406E-4
15-19	2.1794239343342055E-4
20-24	1.7982319154012267E-4
25-29	2.8340797929364954E-4
30-34	7.781289256044939E-4
35-39	7.275795491807728E-4
40-44	0.0010499354086697483
45-49	8.609967558073153E-4
50-54	5.560431406609322E-4
55-59	2.9418079722001635E-4
60-64	2.6600573495105703E-4
65-69	3.190411462808628E-4
70-74	3.7207655761066857E-4
75-79	2.477748123064363E-4
80-84	2.809219443875649E-4
85-89	3.803633406309507E-4
90-94	3.7953466232892245E-4
95-99	3.206985028849192E-4
100-104	4.350561085648129E-4
105-109	2.8506533589770595E-4
110-114	2.477748123064363E-4
115-119	2.4114538589021056E-4
120-124	2.419740641922388E-4
125-129	1.549628424792762E-4
130-134	1.54134164177248E-4
135-139	1.715364085198405E-4
140-144	1.5910623398941728E-4
145-149	1.7982319154012267E-4
150	2.527468821186056E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4134818E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.9828057831755
#Duplication Level	Percentage of total
1	38.50082426360576
2	10.482814378187278
3	6.333350295190854
4	4.636846171737855
5	3.4537851100193424
6	2.6845355048277018
7	2.22759222301432
8	1.8867858091424523
9	1.544463968402574
>10	18.2462496249453
>50	4.4532910395124885
>100	4.744628589963583
>500	0.44959743610996455
>1k	0.33274314763851387
>5k	0.022492437701857597
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.65735660405643E-5	0.0	0.0	1.4087531134479654E-4	1.2430174530423225E-5
2	4.143391510141075E-6	1.160149622839501E-4	0.0	0.0	0.0019101034861750356	1.65735660405643E-5
3	4.143391510141075E-6	1.2430174530423226E-4	0.0	0.0	0.003032962585423267	5.3864089631833976E-5
4	2.9003740570987526E-5	1.4087531134479654E-4	0.0	4.143391510141075E-6	0.004479006222462502	6.62942641622572E-5
5	4.143391510141075E-5	1.4501870285493762E-4	0.0	4.143391510141075E-6	0.0057220236755048245	7.043765567239827E-5
6	5.800748114197505E-5	1.4501870285493762E-4	0.0	4.143391510141075E-6	0.006890460081364608	8.28678302028215E-5
7	6.215087265211613E-5	1.9473940097663051E-4	0.0	4.143391510141075E-6	0.00801746257212298	8.28678302028215E-5
8	7.043765567239827E-5	1.988827924867716E-4	0.0	4.143391510141075E-6	0.010072584761152954	8.701122171296258E-5
9	8.28678302028215E-5	1.988827924867716E-4	0.0	4.143391510141075E-6	0.013673191983465548	9.94413962433858E-5
10-11	1.325885283245144E-4	2.092412712621243E-4	0.0	4.143391510141075E-6	0.018338650823884396	1.4709039861000818E-4
12-13	1.9681109673170107E-4	2.1959975003747696E-4	0.0	8.28678302028215E-6	0.02220029171133588	1.988827924867716E-4
14-15	2.0716957550705376E-4	2.3410162032297074E-4	0.0	1.2430174530423225E-5	0.026567426363024574	2.2581483730268857E-4
16-17	2.3824501183311182E-4	2.548185778736761E-4	0.0	1.65735660405643E-5	0.030928345927448052	2.320299245679002E-4
18-19	2.6724875240409935E-4	2.713921439142404E-4	0.0	1.65735660405643E-5	0.03458488893514755	2.423884033432529E-4
20-21	3.335430165663565E-4	3.418297995866387E-4	0.0	1.65735660405643E-5	0.03815442072113409	2.651770566490288E-4
22-23	4.2262593403438964E-4	3.542599741170619E-4	0.0	1.65735660405643E-5	0.04206578230670727	2.817506226895931E-4
24-25	5.117088515024228E-4	3.5633166987213247E-4	0.0	2.278865330577591E-5	0.04630032843007144	2.9003740570987524E-4
26-27	5.469276793386218E-4	3.5633166987213247E-4	0.0	2.9003740570987526E-5	0.05052865946617041	3.273279293011449E-4
28-29	5.717880283994684E-4	3.5633166987213247E-4	0.0	3.31471320811286E-5	0.054914439379654734	3.5011658260692086E-4
30-31	6.256521180313024E-4	3.5840336562720297E-4	0.0	4.7649002366622364E-5	0.059364441861546255	3.749769316677673E-4
32-33	6.650143373776425E-4	3.687618444025557E-4	0.0	5.593578538690452E-5	0.06325715818532379	4.184825425242486E-4
34-35	7.416670803152524E-4	3.7290523591269675E-4	0.0	7.66527429376099E-5	0.06663816565759892	4.288410212996013E-4
36-37	7.582406463558167E-4	3.811920189329789E-4	0.0	7.872443869268042E-5	0.06987622612277417	4.4955797885030664E-4
38-39	7.74814212396381E-4	3.915504977083316E-4	0.0	7.872443869268042E-5	0.07416877972728031	4.764900236662236E-4
40-41	8.307499977832856E-4	3.9362219346340214E-4	0.0	8.493952595789203E-5	0.07752078345898444	4.827051109314352E-4
42-43	8.369650850484972E-4	3.977655849735432E-4	0.0	9.94413962433858E-5	0.08093079467183055	4.8477680668650576E-4
44-45	8.597537383542731E-4	4.2676932554453075E-4	0.0	9.94413962433858E-5	0.08504932583291078	4.930635897067879E-4
46-47	9.053310449658249E-4	4.2676932554453075E-4	0.0	9.94413962433858E-5	0.08909949103407368	4.97206981216929E-4
48-49	9.36406481291883E-4	4.371278043198834E-4	0.0	9.94413962433858E-5	0.09297563379181065	5.137805472574933E-4
50-51	9.529800473324472E-4	4.474862830952361E-4	0.0	9.94413962433858E-5	0.09694293116277072	5.158522430125639E-4
52-53	0.0010151309199845633	4.474862830952361E-4	0.0	1.0151309199845634E-4	0.10113604337103349	5.179239387676344E-4
54-55	0.0010627799223511857	4.474862830952361E-4	0.0	1.0358478775352688E-4	0.10436581705318847	5.220673302777755E-4
56-57	0.001110428924717808	4.66131544890871E-4	0.0	1.0358478775352688E-4	0.1083082540750877	5.365692005632692E-4
58-59	0.001133217578023584	5.0135037272707E-4	0.0	1.0772817926366795E-4	0.11224447600972173	5.489993750936924E-4
60-61	0.0011808665803902064	5.179239387676344E-4	0.0	1.1187157077380902E-4	0.11570835131219966	5.655729411342568E-4
62-63	0.001240945757287252	5.262107217879165E-4	0.0	1.1187157077380902E-4	0.11894641177737492	5.676446368893273E-4
64-65	0.0012616627148379573	5.303541132980576E-4	0.0	1.1601496228395009E-4	0.12331561812481867	5.676446368893273E-4
66-67	0.0012844513681437332	5.386408963183397E-4	0.0	1.2015835379409118E-4	0.1270964628778224	5.738597241545389E-4
68-69	0.0013631758068364138	5.448559835835513E-4	0.0	1.2223004954916173E-4	0.13011906698447032	5.966483774603148E-4
70-71	0.0014854058563855753	5.635012453791862E-4	0.0	1.2430174530423226E-4	0.1328619921641837	5.966483774603148E-4
72-73	0.0015413416417724799	5.635012453791862E-4	0.0	1.2430174530423226E-4	0.13567742669532457	6.007917689704559E-4
74-75	0.0016097076016898077	5.676446368893273E-4	0.0	1.2844513681437331E-4	0.13884919289633757	6.152936392559497E-4
76-77	0.0016656433870767123	5.676446368893273E-4	0.0	1.3051683256944384E-4	0.14125857505948458	6.173653350110202E-4
78-79	0.0017340093469940397	5.717880283994684E-4	0.0	1.4087531134479654E-4	0.14370939113773307	6.173653350110202E-4
80-81	0.0017671564790751686	5.759314199096094E-4	0.0	1.4294700709986707E-4	0.14624307504618433	6.235804222762319E-4
82-83	0.0018106620899316498	5.82146507174821E-4	0.0	1.4501870285493762E-4	0.1489217776574905	6.256521180313024E-4
84-85	0.0018831714413591184	5.945766817052442E-4	0.0	1.4709039861000818E-4	0.15139331069328965	6.256521180313024E-4
86-87	0.0019639675758068697	6.049351604805969E-4	0.0	1.491620943650787E-4	0.15354787427856303	6.256521180313024E-4
88-89	0.0020281901442140565	6.132219435008792E-4	0.0	1.533054858752198E-4	0.15578116230252909	6.297955095414434E-4
90-91	0.0020530504932749025	6.194370307660907E-4	0.0	1.533054858752198E-4	0.15864010244452642	6.360105968066551E-4
92-93	0.0020758391465806785	6.215087265211613E-4	0.0	1.533054858752198E-4	0.16131466166432248	6.422256840718666E-4
94-95	0.002146276802253077	6.235804222762319E-4	0.0	1.533054858752198E-4	0.16431862050917476	6.505124670921488E-4
96-97	0.002227072936700828	6.339389010515845E-4	0.0	1.533054858752198E-4	0.16688337985395207	6.505124670921488E-4
98-99	0.00231201246265872	6.339389010515845E-4	0.0	1.533054858752198E-4	0.16890949830241106	6.546558586022898E-4
100-101	0.0024238840334325287	6.339389010515845E-4	0.0	1.533054858752198E-4	0.17091904318482948	6.546558586022898E-4
102-103	0.0024673896442890106	6.339389010515845E-4	0.0	1.533054858752198E-4	0.17279185614741324	6.608709458675014E-4
104-105	0.0025253971254309854	6.339389010515845E-4	0.0	1.5537718163029032E-4	0.17463566536942604	6.67086033132713E-4
106-107	0.0026227668259193003	6.422256840718666E-4	2.0716957550705375E-6	1.6159226889550193E-4	0.1763033804522578	6.712294246428542E-4
108-109	0.002726351613672827	6.422256840718666E-4	4.143391510141075E-6	1.65735660405643E-4	0.17801667284170114	6.712294246428542E-4
110-111	0.002798860965100296	6.587992501124309E-4	4.143391510141075E-6	1.65735660405643E-4	0.17967817283726772	6.712294246428542E-4
112-113	0.00285272505473213	6.62942641622572E-4	4.143391510141075E-6	1.65735660405643E-4	0.18140596709699655	6.712294246428542E-4
114-115	0.0029086608401190346	6.712294246428542E-4	4.143391510141075E-6	1.7609413918099568E-4	0.18341136858790483	6.712294246428542E-4
116-117	0.002960453233995798	6.774445119080658E-4	4.143391510141075E-6	1.9681109673170107E-4	0.18517852506698002	6.712294246428542E-4
118-119	0.0030184607151377734	6.89874686438489E-4	4.143391510141075E-6	2.0302618399691268E-4	0.18665978753185541	6.712294246428542E-4
120-121	0.003153120939217358	6.919463821935595E-4	4.143391510141075E-6	2.154563585273359E-4	0.18812861982220044	6.712294246428542E-4
122-123	0.0033084981208476487	6.9401807794863E-4	4.143391510141075E-6	2.2167144579254752E-4	0.18966167468095263	6.753728161529953E-4
124-125	0.0033913659510504696	6.960897737037006E-4	4.143391510141075E-6	2.2581483730268857E-4	0.191169869190644	6.753728161529953E-4
126-127	0.0034245130831315985	7.043765567239827E-4	4.143391510141075E-6	2.2788653305775913E-4	0.1925765506083369	6.753728161529953E-4
128-129	0.0034783771727634324	7.043765567239827E-4	4.143391510141075E-6	2.2788653305775913E-4	0.19401223576660076	6.795162076631363E-4
130-131	0.0035467431326807602	7.043765567239827E-4	4.143391510141075E-6	2.2788653305775913E-4	0.19548313975270085	6.795162076631363E-4
132-133	0.0036337543543937226	7.064482524790532E-4	4.143391510141075E-6	2.320299245679002E-4	0.19674894585904895	6.795162076631363E-4
134-135	0.0037953466232892247	7.085199482341238E-4	4.143391510141075E-6	2.320299245679002E-4	0.19792359735217396	6.857312949283479E-4
136-137	0.0039382936303890915	7.168067312544059E-4	4.143391510141075E-6	2.320299245679002E-4	0.19911896580284966	6.919463821935595E-4
138-139	0.004025304852102054	7.292369057848292E-4	4.143391510141075E-6	2.320299245679002E-4	0.2001195948525487	6.960897737037006E-4
>>END_MODULE
