##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24134818
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.74570800575335	37.0	35.0	38.0	33.0	39.0
2	35.31586569246141	35.0	34.0	37.0	33.0	39.0
3	35.46195612496436	35.0	34.0	37.0	33.0	39.0
4	35.52082820761275	36.0	35.0	38.0	33.0	39.0
5	35.435489424448946	36.0	35.0	37.0	33.0	39.0
6	35.39261352623417	35.0	35.0	37.0	33.0	39.0
7	35.41955456220967	35.0	34.0	37.0	33.0	39.0
8	35.54885899698933	36.0	35.0	38.0	33.0	39.0
9	35.505852706243736	36.0	34.0	38.0	33.0	39.0
10-14	35.48674621039197	35.8	34.4	37.8	33.0	39.0
15-19	35.46541034616462	36.0	34.8	38.0	33.0	39.0
20-24	35.44787107986478	36.0	34.2	38.0	33.0	39.0
25-29	35.42912461158812	36.0	34.0	38.0	33.0	39.0
30-34	35.410294397082254	36.0	34.0	38.0	33.0	39.0
35-39	35.382546709074006	36.0	34.0	38.0	33.0	39.0
40-44	35.364075154824036	36.0	34.0	38.0	33.0	39.0
45-49	35.33472457094974	36.0	34.0	38.0	32.4	39.0
50-54	35.31003434125751	36.0	34.0	38.0	32.0	39.0
55-59	35.28679746414495	36.0	34.0	38.0	32.0	39.0
60-64	35.24517408832335	36.0	34.0	38.0	32.0	39.0
65-69	35.21740632972662	36.0	34.0	38.0	32.0	39.0
70-74	35.18324535117688	35.8	34.0	38.0	32.0	39.0
75-79	35.13944457339599	35.6	34.0	37.8	32.0	39.0
80-84	35.100075873785336	35.2	34.0	37.4	32.0	39.0
85-89	35.04929889257917	35.0	34.0	37.0	32.0	39.0
90-94	34.99251344675564	35.0	34.0	37.0	31.6	39.0
95-99	34.927480986183525	35.0	34.0	37.0	31.0	39.0
100-104	34.87950805346865	35.0	34.0	37.0	31.0	39.0
105-109	34.816324556497584	35.0	34.0	37.0	31.0	39.0
110-114	34.74646610552439	35.0	34.0	37.0	31.0	39.0
115-119	34.67543759393586	35.0	34.0	37.0	30.4	39.0
120-124	34.600884746675945	35.0	34.0	37.0	30.0	39.0
125-129	34.521218001312455	35.0	34.0	37.0	30.0	39.0
130-134	34.433709448316534	35.0	34.0	37.0	30.0	39.0
135-139	34.3536621075825	35.0	34.0	37.0	29.8	39.0
140-144	34.22005905327316	35.0	34.0	37.0	29.0	39.0
145-149	34.13672915204912	35.0	34.0	37.0	29.0	39.0
150	34.070655515198	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	19.0
21	343.0
22	2538.0
23	12264.0
24	37566.0
25	86748.0
26	161522.0
27	266206.0
28	381202.0
29	499428.0
30	639948.0
31	830684.0
32	1105731.0
33	1540939.0
34	2311007.0
35	3951883.0
36	1.059334E7
37	1713443.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.63342122571631	18.733321295399865	16.018198272719523	31.615059206164304
2	24.455121227763144	25.44210608921932	33.36954105060995	16.733231632407588
3	19.736017897462496	29.526910043407	30.299155353067093	20.437916706063415
4	23.39225429419024	34.1354759750001	22.478541168199403	19.99372856261025
5	25.167979306908382	35.22192294965721	22.51283187633733	17.09726586709707
6	18.7874298451308	39.13846377461807	22.819467708436832	19.254638671814305
7	20.226404856253733	20.558058486291465	38.36099364826368	20.854543009191122
8	21.457054285638282	23.238339729763034	28.48350047636572	26.82110550823296
9	23.521126200330162	23.109911166514703	28.838899883148073	24.530062750007065
10-14	23.121348584439293	29.246058536675108	25.67729990754436	21.95529297134124
15-19	22.954633426280655	28.316132319705083	27.17451028634233	21.554723967671936
20-24	23.062819035967042	28.37618497889646	27.074093535737454	21.48690244939904
25-29	23.165788944420463	28.354893747282457	26.98456396066463	21.494753347632454
30-34	23.15515948784035	28.14614305357513	27.15951203775392	21.539185420830602
35-39	23.2564299698173	28.11094020357593	27.126046089314173	21.5065837372926
40-44	23.4087416776874	27.866582627637797	27.256521263180854	21.468154431493954
45-49	23.40632442308038	27.704410283930876	27.330873595152035	21.55839169783671
50-54	23.490399637569258	27.669150022179572	27.287430135168204	21.553020205082962
55-59	23.656687197724054	27.550995412519786	27.194804617959	21.597512771797163
60-64	23.58886236473795	27.516603605629015	27.245491554980855	21.64904247465218
65-69	23.665462268374778	27.424647887749952	27.227564982902003	21.682324860973264
70-74	23.727574825714452	27.37888058654513	27.13258330765121	21.760961280089205
75-79	23.641915178312097	27.428041926812956	27.218589342583815	21.711453552291136
80-84	23.76011970556496	27.474194563838804	27.081794667619157	21.68389106297707
85-89	23.794737544737234	27.381768530427703	27.14910052356724	21.674393401267828
90-94	23.736527234110714	27.319808480178377	27.19653678098204	21.747127504728876
95-99	23.797647862933957	27.45806991376525	27.170162211291586	21.574120012009207
100-104	23.969132064720768	27.410652941323193	26.964283716579097	21.65593127737694
105-109	23.74241002142556	27.545006782690525	27.085092807288564	21.62749038859535
110-114	23.78433846072508	27.562214888050946	27.00268466909508	21.650761982128888
115-119	23.859424172993556	27.551239043940583	27.030523287973416	21.558813495092444
120-124	23.834290360093043	27.449537013289266	27.217458196701543	21.49871442991615
125-129	23.82456167682723	27.520921848260883	27.156652268933623	21.497864205978267
130-134	23.865833170981443	27.449793903562895	27.226903471988063	21.4574694534676
135-139	23.801327154862395	27.458161149859468	27.313089406784485	21.427422288493652
140-144	23.820204486315166	27.58884777999983	27.27144078733057	21.319506946354434
145-149	23.928252535403416	28.039176429671027	26.84266606029513	21.189904974630426
150	23.538536731455775	28.01944891401294	26.963600885658224	21.47841346887306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	2.0
9	1.5
10	3.5
11	6.0
12	8.5
13	13.0
14	25.5
15	46.5
16	81.5
17	145.5
18	245.0
19	439.5
20	661.5
21	1015.5
22	1592.0
23	2653.5
24	4353.5
25	7197.0
26	11403.5
27	18130.0
28	29679.0
29	45606.5
30	61156.5
31	79446.5
32	104634.0
33	137097.0
34	184552.0
35	255064.5
36	354986.5
37	481275.5
38	629353.0
39	814293.5
40	1029641.0
41	1227497.5
42	1407612.0
43	1577308.5
44	1696287.0
45	1747442.5
46	1743700.5
47	1687376.5
48	1580400.5
49	1430970.5
50	1242942.5
51	1042821.0
52	850239.0
53	674621.5
54	527050.0
55	400356.5
56	297419.0
57	220535.5
58	160894.5
59	114722.5
60	78943.5
61	52922.5
62	35421.5
63	23995.0
64	16428.5
65	11241.5
66	7916.5
67	6122.5
68	5558.0
69	4207.0
70	2399.5
71	1681.0
72	966.0
73	702.0
74	581.0
75	357.5
76	246.5
77	91.0
78	22.0
79	1.5
80	1.0
81	0.5
82	1.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.286783020282149E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.286783020282149E-7
70-74	0.0
75-79	0.0
80-84	8.286783020282149E-7
85-89	0.0
90-94	8.286783020282149E-7
95-99	0.0
100-104	0.0
105-109	8.286783020282149E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	3.3147132081128598E-6
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4134818E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.27525710827136
#Duplication Level	Percentage of total
1	28.921036035403546
2	10.73305719425167
3	7.013058743438341
4	5.037089277027975
5	3.924646240614582
6	3.1531279703723754
7	2.5419516965171844
8	2.1122785132980026
9	1.859613528721664
>10	20.596441625989197
>50	5.3210748912952885
>100	6.547256913040863
>500	1.1013186177469116
>1k	1.000834685838146
>5k	0.08025534776315163
>10k+	0.05695871868100914
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.65735660405643E-5	1.2430174530423225E-5	0.0	0.0	5.800748114197505E-4	0.0
2	3.31471320811286E-5	4.557730661155182E-5	0.0	0.0	0.004901632156496891	0.0
3	7.043765567239827E-5	4.557730661155182E-5	0.0	0.0	0.006492694496391065	0.0
4	1.160149622839501E-4	4.557730661155182E-5	0.0	0.0	0.00802160596363312	0.0
5	1.2430174530423226E-4	4.97206981216929E-5	4.143391510141075E-6	0.0	0.009641672044098281	0.0
6	1.325885283245144E-4	4.97206981216929E-5	4.143391510141075E-6	0.0	0.011199587251911326	0.0
7	1.65735660405643E-4	5.800748114197505E-5	4.143391510141075E-6	0.0	0.013267139615471723	0.0
8	2.444600990983234E-4	5.800748114197505E-5	4.143391510141075E-6	0.0	0.018935299201344714	0.0
9	2.6103366513888774E-4	5.800748114197505E-5	4.143391510141075E-6	0.0	0.02502194132974195	0.0
10-11	2.8796570995480474E-4	6.007917689704559E-5	1.2430174530423225E-5	4.143391510141075E-6	0.03154571126245907	0.0
12-13	3.211128420359333E-4	6.215087265211613E-5	1.2430174530423225E-5	4.143391510141075E-6	0.03770486274228378	0.0
14-15	3.832637146880494E-4	7.872443869268042E-5	1.2430174530423225E-5	6.215087265211612E-6	0.04627753977676567	0.0
16-17	4.309127170546718E-4	7.872443869268042E-5	1.4501870285493761E-5	8.28678302028215E-6	0.05198713327774007	0.0
18-19	5.179239387676344E-4	8.28678302028215E-5	1.65735660405643E-5	8.28678302028215E-6	0.05978085270831543	0.0
20-21	6.152936392559497E-4	8.908291746803312E-5	1.65735660405643E-5	8.28678302028215E-6	0.06579084209377506	0.0
22-23	6.815879034182068E-4	1.0151309199845634E-4	1.65735660405643E-5	8.28678302028215E-6	0.07212194432127061	0.0
24-25	7.47882167580464E-4	1.0979987501873848E-4	1.65735660405643E-5	8.28678302028215E-6	0.07720381400845866	0.0
26-27	7.851726911717337E-4	1.1187157077380902E-4	1.65735660405643E-5	1.0358478775352688E-5	0.08134099043133451	0.0
28-29	9.840554836585053E-4	1.1187157077380902E-4	1.65735660405643E-5	1.2430174530423225E-5	0.08583449852408251	0.0
30-31	0.0012347306700220403	1.3466022407958495E-4	2.0716957550705376E-5	1.2430174530423225E-5	0.09092051160278067	0.0
32-33	0.0014336134625088119	1.4709039861000818E-4	2.0716957550705376E-5	1.2430174530423225E-5	0.09598787941968322	0.0
34-35	0.0016138509931999487	1.5744887738536084E-4	2.0716957550705376E-5	1.2430174530423225E-5	0.09900219674331084	0.0
36-37	0.0016635716913216416	1.7402244342592515E-4	2.9003740570987526E-5	1.2430174530423225E-5	0.1023728457368106	2.0716957550705375E-6
38-39	0.0017091489979331933	1.7609413918099568E-4	2.9003740570987526E-5	1.2430174530423225E-5	0.10564198163831193	4.143391510141075E-6
40-41	0.0017754432620954506	1.8023753069113676E-4	2.9003740570987526E-5	1.4501870285493761E-5	0.10848227651851364	4.143391510141075E-6
42-43	0.001792016828136015	1.9059600946648946E-4	2.9003740570987526E-5	1.65735660405643E-5	0.11160225032564985	4.143391510141075E-6
44-45	0.0018562393965432017	2.2374314154761807E-4	2.9003740570987526E-5	1.65735660405643E-5	0.11398677213973604	4.143391510141075E-6
46-47	0.0019266770522155998	2.8175062268959313E-4	2.9003740570987526E-5	1.65735660405643E-5	0.1164935240033714	4.143391510141075E-6
48-49	0.001992971316377857	2.9418079722001635E-4	2.9003740570987526E-5	1.65735660405643E-5	0.11882625342358083	4.143391510141075E-6
50-51	0.0020344052314792676	3.0039588448522796E-4	2.9003740570987526E-5	1.65735660405643E-5	0.1209580283555484	8.28678302028215E-6
52-53	0.0020882693211111015	3.1696945052579224E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.12318302959649416	1.0358478775352688E-5
54-55	0.0021442051064980063	3.4390149534170925E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.12515942734683144	1.2430174530423225E-5
56-57	0.002229144632455898	3.4597319109677975E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.12710474966084268	1.2430174530423225E-5
58-59	0.002289223809352944	3.542599741170619E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.12891955514228448	1.2430174530423225E-5
60-61	0.002378306726820977	3.708335401576262E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.1304360364349961	1.2430174530423225E-5
62-63	0.0024176689461673172	3.811920189329789E-4	2.9003740570987526E-5	2.278865330577591E-5	0.13230263431031467	1.2430174530423225E-5
64-65	0.0024487443824933756	3.9569388921847264E-4	2.9003740570987526E-5	2.486034906084645E-5	0.13399313804645224	1.2430174530423225E-5
66-67	0.0025419706914715495	4.101957595039664E-4	2.9003740570987526E-5	2.486034906084645E-5	0.1353024497636568	1.2430174530423225E-5
68-69	0.0025606159532671844	4.164108467691781E-4	2.9003740570987526E-5	2.9003740570987526E-5	0.13667598404926856	1.2430174530423225E-5
70-71	0.0025916913895932423	4.226259340343897E-4	3.31471320811286E-5	3.31471320811286E-5	0.1380868088584716	1.2430174530423225E-5
72-73	0.0026455554792250762	4.4334289158509503E-4	3.7290523591269676E-5	3.7290523591269676E-5	0.13890720037747956	1.2430174530423225E-5
74-75	0.0026662724367757817	4.4541458734016553E-4	4.143391510141075E-5	3.7290523591269676E-5	0.13995547842954523	1.2430174530423225E-5
76-77	0.002713921439142404	4.5784476187058876E-4	4.143391510141075E-5	3.7290523591269676E-5	0.14126271845099475	1.2430174530423225E-5
78-79	0.002759498745753956	4.744183279111531E-4	4.143391510141075E-5	3.7290523591269676E-5	0.14283306383333821	1.2430174530423225E-5
80-81	0.00277607231179452	4.889201981966468E-4	4.557730661155182E-5	3.7290523591269676E-5	0.1441713792911138	1.2430174530423225E-5
82-83	0.002794717573590155	5.054937642372111E-4	4.557730661155182E-5	3.7290523591269676E-5	0.14571064923713117	1.2430174530423225E-5
84-85	0.002821649618406072	5.096371557473521E-4	4.97206981216929E-5	3.7290523591269676E-5	0.14737422092845281	1.65735660405643E-5
86-87	0.0028506533589770598	5.137805472574933E-4	4.97206981216929E-5	3.936221934634021E-5	0.14909580010091644	1.65735660405643E-5
88-89	0.0028568684462422713	5.179239387676344E-4	5.3864089631833976E-5	4.143391510141075E-5	0.15081530757762499	1.65735660405643E-5
90-91	0.0028672269250176238	5.262107217879165E-4	5.3864089631833976E-5	4.350561085648129E-5	0.1517496423631618	1.8645261795634838E-5
92-93	0.0028775854037929767	5.303541132980576E-4	5.593578538690452E-5	4.557730661155182E-5	0.15276477328314636	2.0716957550705376E-5
94-95	0.0029045174486088936	5.407125920734103E-4	5.800748114197505E-5	5.3864089631833976E-5	0.15378404759464107	2.486034906084645E-5
96-97	0.002910732535874105	5.531427666038335E-4	5.800748114197505E-5	5.3864089631833976E-5	0.1545837221560983	2.486034906084645E-5
98-99	0.002921091014649458	5.593578538690451E-4	6.215087265211613E-5	5.3864089631833976E-5	0.15541861554539171	2.9003740570987526E-5
100-101	0.002921091014649458	5.593578538690451E-4	6.215087265211613E-5	5.3864089631833976E-5	0.1562141467153388	2.9003740570987526E-5
102-103	0.002933521189179881	5.593578538690451E-4	6.215087265211613E-5	5.3864089631833976E-5	0.15706147027916267	2.9003740570987526E-5
104-105	0.0029418079722001635	5.635012453791862E-4	6.215087265211613E-5	5.3864089631833976E-5	0.15790050705996622	2.9003740570987526E-5
106-107	0.0029625249297508685	5.697163326443978E-4	6.215087265211613E-5	5.3864089631833976E-5	0.15870225331717852	2.9003740570987526E-5
108-109	0.0029728834085262215	5.987200732153854E-4	6.62942641622572E-5	5.3864089631833976E-5	0.1595350750107169	3.1075436326058065E-5
110-111	0.002997743757587068	6.152936392559497E-4	6.62942641622572E-5	5.3864089631833976E-5	0.16041761740237692	3.7290523591269676E-5
112-113	0.0030039588448522794	6.277238137863729E-4	6.62942641622572E-5	5.593578538690452E-5	0.1612214353553443	4.143391510141075E-5
114-115	0.0030039588448522794	6.380822925617256E-4	6.62942641622572E-5	6.215087265211613E-5	0.16207704570218845	4.143391510141075E-5
116-117	0.0030039588448522794	6.422256840718666E-4	6.62942641622572E-5	6.215087265211613E-5	0.16301759557499046	4.350561085648129E-5
118-119	0.003014317323627632	6.608709458675015E-4	7.043765567239827E-5	6.215087265211613E-5	0.1638027682661622	4.557730661155182E-5
120-121	0.0030163890193827024	6.62942641622572E-4	7.043765567239827E-5	6.215087265211613E-5	0.16467702387480196	4.557730661155182E-5
122-123	0.0030226041066479144	6.62942641622572E-4	7.043765567239827E-5	6.215087265211613E-5	0.16544562299993312	4.557730661155182E-5
124-125	0.0030267474981580554	6.650143373776425E-4	7.043765567239827E-5	6.215087265211613E-5	0.16630952012979755	4.557730661155182E-5
126-127	0.0030371059769334083	6.753728161529953E-4	7.043765567239827E-5	6.215087265211613E-5	0.1673453680073328	4.557730661155182E-5
128-129	0.0030578229344841133	6.815879034182069E-4	7.043765567239827E-5	6.215087265211613E-5	0.1683874309721333	4.557730661155182E-5
130-131	0.0030743965005246773	7.085199482341238E-4	7.043765567239827E-5	6.215087265211613E-5	0.16940049019636277	4.97206981216929E-5
132-133	0.003099256849585524	7.20950122764547E-4	7.043765567239827E-5	6.215087265211613E-5	0.17020637984508524	5.800748114197505E-5
134-135	0.0031116870241159472	7.395953845601819E-4	7.458104718253935E-5	6.215087265211613E-5	0.17105163171315402	5.800748114197505E-5
136-137	0.003117902111381159	7.437387760703229E-4	7.458104718253935E-5	6.215087265211613E-5	0.17193624580056913	5.800748114197505E-5
138-139	0.003144834156197076	7.540972548456756E-4	7.458104718253935E-5	6.215087265211613E-5	0.17285193532431029	5.800748114197505E-5
>>END_MODULE
