##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23585421
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.539316130926814	36.0	35.0	38.0	32.0	39.0
2	35.210375850403516	35.0	34.0	37.0	32.0	39.0
3	35.35234274597006	35.0	34.0	37.0	33.0	39.0
4	35.40245565258301	36.0	35.0	37.0	33.0	39.0
5	35.317314963341126	35.0	34.0	37.0	33.0	39.0
6	35.28287750301341	35.0	34.0	37.0	33.0	39.0
7	35.31173609324167	35.0	34.0	37.0	33.0	39.0
8	35.38539006787286	36.0	34.0	38.0	33.0	39.0
9	35.38675642041751	36.0	34.0	38.0	33.0	39.0
10-14	35.37108873316275	35.4	34.2	37.4	33.0	39.0
15-19	35.34746244300664	35.8	34.0	37.2	33.0	39.0
20-24	35.33023673395527	35.6	34.0	37.0	32.8	39.0
25-29	35.31092445625626	35.8	34.0	37.0	32.0	39.0
30-34	35.29025696848914	35.6	34.0	37.0	32.0	39.0
35-39	35.263935615141236	35.4	34.0	37.0	32.0	39.0
40-44	35.24197665159337	35.4	34.0	37.0	32.0	39.0
45-49	35.212966221802866	35.0	34.0	37.0	32.0	39.0
50-54	35.18769751873414	35.0	34.0	37.0	32.0	39.0
55-59	35.16121437052151	35.0	34.0	37.0	32.0	39.0
60-64	35.117435978776896	35.0	34.0	37.0	32.0	39.0
65-69	35.08955947828958	35.0	34.0	37.0	32.0	39.0
70-74	35.05629886360731	35.0	34.0	37.0	32.0	39.0
75-79	35.00682868455051	35.0	34.0	37.0	32.0	39.0
80-84	34.96513761615704	35.0	34.0	37.0	31.4	39.0
85-89	34.91246489939696	35.0	34.0	37.0	31.0	39.0
90-94	34.85524396617724	35.0	34.0	37.0	31.0	39.0
95-99	34.78821388857125	35.0	34.0	37.0	31.0	39.0
100-104	34.73621531708084	35.0	34.0	37.0	31.0	39.0
105-109	34.67305051709698	35.0	34.0	37.0	30.8	39.0
110-114	34.595921760311164	35.0	34.0	37.0	30.0	39.0
115-119	34.530168691922015	35.0	34.0	37.0	30.0	39.0
120-124	34.454172371992	35.0	34.0	37.0	30.0	39.0
125-129	34.37263143193416	35.0	34.0	37.0	30.0	39.0
130-134	34.26907082981474	35.0	34.0	37.0	29.4	39.0
135-139	34.20550115259762	35.0	34.0	37.0	29.0	39.0
140-144	34.073918553330046	35.0	34.0	37.0	29.0	39.0
145-149	33.994537048967665	35.0	34.0	37.0	28.4	39.0
150	33.92653253889341	35.0	33.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	21.0
21	316.0
22	2449.0
23	11935.0
24	37811.0
25	89914.0
26	171188.0
27	282558.0
28	406827.0
29	530963.0
30	681190.0
31	879917.0
32	1170642.0
33	1622142.0
34	2406436.0
35	4050004.0
36	9788867.0
37	1452238.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.65762353786265	19.335156239102112	16.839474690742218	31.167745532293022
2	23.752329882091143	25.791233491231726	33.24418080135182	17.212255825325315
3	19.807299602580763	29.62747198788608	30.420741694625676	20.144486714907483
4	23.173442611009573	34.2451381300338	22.438484350141554	20.142934908815068
5	25.02870735273286	35.26616718014065	22.577816185685215	17.127309281441278
6	18.789683677895763	39.16877294664361	22.792118063103477	19.249425312357154
7	20.17689656673926	20.533095423651755	38.51232929020008	20.77767871940891
8	21.453170072980182	23.0981404409646	28.57158199458791	26.877107491467306
9	23.48239194034315	22.931759411884148	28.887506396430236	24.69834225134247
10-14	22.977967618216354	29.090295229413123	25.8629905313117	22.068746621058832
15-19	22.82370113300076	28.24242314775725	27.29864944959007	21.635226269651916
20-24	22.91435459218642	28.389520797614765	27.12352262018134	21.57260199001748
25-29	23.037857157605963	28.339867242564804	27.040336485831652	21.581939113997585
30-34	23.044523139951583	28.152283565343183	27.176335754193236	21.626857540511995
35-39	23.15226107816571	28.110719143921315	27.13145995682214	21.605559821090832
40-44	23.276401977306236	27.889271936252484	27.262595821376262	21.571730265065018
45-49	23.299784218394915	27.711042342640397	27.33080490698046	21.65836853198423
50-54	23.377729827252182	27.667439135387916	27.29547460696165	21.65935643039825
55-59	23.54034488967015	27.56159601782471	27.19703945244004	21.701019640065102
60-64	23.46786092985154	27.522572524781303	27.25166364424871	21.75790290111845
65-69	23.54076359289919	27.432592362883835	27.22818134134642	21.79846270287056
70-74	23.61093829955378	27.38388685111875	27.132767314181077	21.872407535146394
75-79	23.53546964457408	27.450900282848462	27.21029147624713	21.803338596330335
80-84	23.650243936709884	27.47505333909452	27.080538439402886	21.79416428479271
85-89	23.67660513670712	27.39609524036056	27.154920830117895	21.772378792814425
90-94	23.612791139068495	27.33608443962056	27.199136279992626	21.85198814131832
95-99	23.698640783219428	27.45535642547996	27.17769761243609	21.668305178864518
100-104	23.855019792566093	27.42593085301238	26.977647140389372	21.741402214032153
105-109	23.625160644789847	27.570302009872965	27.07613317565966	21.728404169677532
110-114	23.661566521891384	27.568399993947622	27.00049524993634	21.769538234224655
115-119	23.74315831583552	27.558952011574878	27.019004045075167	21.678885627514436
120-124	23.713412620448878	27.45992110973978	27.21025331708092	21.616412952730418
125-129	23.694086475658725	27.535616409269238	27.163640005948082	21.606657109123955
130-134	23.73141546014831	27.446593560795502	27.243334296257366	21.578656682798826
135-139	23.672327070184586	27.451645658561702	27.325170918085373	21.550856353168342
140-144	23.69354611054007	27.561448235331476	27.303041145629752	21.441964508498703
145-149	23.78151146846181	27.999019394226625	26.900189740094106	21.319279397217457
150	23.43303517880813	27.957041767454566	27.024614061372915	21.585308992364393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.5
8	3.5
9	5.0
10	3.5
11	4.5
12	11.5
13	16.0
14	23.0
15	42.5
16	81.0
17	155.5
18	251.0
19	427.5
20	658.0
21	1022.0
22	1618.5
23	2703.5
24	4422.5
25	7102.5
26	11316.0
27	18210.5
28	30226.0
29	45969.5
30	61142.5
31	79501.5
32	104858.5
33	137612.5
34	184659.5
35	254604.0
36	353859.0
37	477383.5
38	623333.5
39	805419.5
40	1014040.0
41	1206913.5
42	1381767.0
43	1543893.5
44	1654866.5
45	1701740.5
46	1696514.0
47	1637633.0
48	1530519.0
49	1385005.5
50	1203070.0
51	1008374.0
52	824830.0
53	658973.5
54	516186.5
55	393616.0
56	293335.0
57	216758.5
58	157408.5
59	112547.5
60	77848.0
61	51652.5
62	34410.0
63	23725.0
64	16094.5
65	10617.0
66	7380.0
67	5477.5
68	4620.0
69	3400.0
70	1974.0
71	1305.5
72	702.5
73	499.5
74	448.0
75	317.0
76	204.5
77	75.5
78	23.0
79	4.0
80	1.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.11783974515443248
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.479814712656602E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	8.479814712656602E-7
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.479814712656602E-7
105-109	0.0
110-114	0.01950442182058145
115-119	8.479814712656602E-7
120-124	0.0
125-129	8.479814712656602E-7
130-134	0.04829678469593568
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3585421E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.87643505586933
#Duplication Level	Percentage of total
1	29.46376108468931
2	10.68544989061838
3	7.134206258984724
4	5.13793801481107
5	3.9779750763802455
6	3.1815655227317765
7	2.493242926637674
8	2.1781412705467815
9	1.8212400713776111
>10	20.695199658524782
>50	5.131166160535025
>100	6.039153396114121
>500	1.0057066743500453
>1k	0.9361109745807543
>5k	0.06455294668711643
>10k+	0.05459007243066584
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	7.759030462080791E-4	0.0
2	0.0	0.0	0.0	0.0	0.005083648920237633	0.0
3	2.5439444137969808E-5	8.479814712656603E-6	0.0	0.0	0.006732972881849342	0.0
4	8.479814712656603E-5	8.479814712656603E-6	0.0	4.239907356328301E-6	0.008199980827138935	0.0
5	8.903805448289432E-5	8.479814712656603E-6	0.0	4.239907356328301E-6	0.009912903399095568	0.0
6	8.903805448289432E-5	8.479814712656603E-6	0.0	4.239907356328301E-6	0.011625825971052202	0.0
7	9.327796183922263E-5	2.5439444137969808E-5	0.0	8.479814712656603E-6	0.013788178722779636	0.0
8	1.4415685011516223E-4	2.967935149429811E-5	0.0	8.479814712656603E-6	0.01916862115796025	0.0
9	1.4415685011516223E-4	4.2399073563283013E-5	0.0	8.479814712656603E-6	0.025371605620268554	0.0
10-11	1.759561552876245E-4	4.2399073563283013E-5	0.0	8.479814712656603E-6	0.03195830169832457	0.0
12-13	2.1623527517274338E-4	4.2399073563283013E-5	0.0	8.479814712656603E-6	0.038210045095230655	0.0
14-15	2.628742560923547E-4	4.6638980919611315E-5	0.0	8.479814712656603E-6	0.04721560832007196	0.0
16-17	2.9891346862114524E-4	5.511879563226792E-5	0.0	8.479814712656603E-6	0.053270196024908775	0.0
18-19	3.476724032189207E-4	5.723874931043207E-5	0.0	8.479814712656603E-6	0.06088930954423073	0.0
20-21	3.9007147678220375E-4	5.935870298859622E-5	0.0	8.479814712656603E-6	0.06693753738803306	0.0
22-23	4.3247055034548675E-4	8.055823977023772E-5	0.0	8.479814712656603E-6	0.07367899008459505	0.0
24-25	4.79109531265098E-4	8.691810080473017E-5	0.0	8.479814712656603E-6	0.07929262742437374	0.0
26-27	5.066689290812319E-4	9.751786919555092E-5	0.0	8.479814712656603E-6	0.08341381737472484	0.0
28-29	6.084267056331112E-4	1.0175777655187923E-4	0.0	8.479814712656603E-6	0.08795899806070878	0.0
30-31	8.03462444024213E-4	1.1023759126453582E-4	4.239907356328301E-6	8.479814712656603E-6	0.09305748665669356	0.0
32-33	9.921383213808226E-4	1.1871740597719244E-4	4.239907356328301E-6	1.2719722068984904E-5	0.09818565460417264	0.0
34-35	0.0011468949398868054	1.2507726701168487E-4	4.239907356328301E-6	1.4839675747149055E-5	0.10136558512141886	0.0
36-37	0.0011914139671282526	1.3143712804617735E-4	4.239907356328301E-6	1.6959629425313205E-5	0.10491650753234381	0.0
38-39	0.0012359329943696998	1.3143712804617735E-4	4.239907356328301E-6	1.6959629425313205E-5	0.10836779212039505	0.0
40-41	0.0012783320679329829	1.3779698908066978E-4	4.239907356328301E-6	1.6959629425313205E-5	0.11122548967856033	0.0
42-43	0.0013037715120709527	1.4839675747149055E-4	4.239907356328301E-6	1.6959629425313205E-5	0.11427610302143854	0.0
44-45	0.0013419306782779072	1.632364332186396E-4	4.239907356328301E-6	1.6959629425313205E-5	0.11682004743523552	0.0
46-47	0.001407649242300996	1.8867587735660942E-4	4.239907356328301E-6	1.6959629425313205E-5	0.11933855240489452	0.0
48-49	0.0014670079452895923	2.0139559942559433E-4	4.239907356328301E-6	1.6959629425313205E-5	0.12182949797673741	0.0
50-51	0.0015348464629908452	2.0987541413825092E-4	4.239907356328301E-6	1.6959629425313205E-5	0.12392613216444176	0.0
52-53	0.0015963251196576054	2.183552288509075E-4	4.239907356328301E-6	1.6959629425313205E-5	0.1261351238970888	0.0
54-55	0.00165992373000253	2.3107495091989242E-4	4.239907356328301E-6	1.6959629425313205E-5	0.12796888382870078	0.0
56-57	0.0017828810433360505	2.39554765632549E-4	4.239907356328301E-6	1.9079583103477356E-5	0.12979628389927828	2.1199536781641507E-6
58-59	0.0018379998389683185	2.565143950578622E-4	4.239907356328301E-6	2.1199536781641507E-5	0.13160248443307415	4.239907356328301E-6
60-61	0.0019355177081638694	2.6923411712684715E-4	4.239907356328301E-6	2.1199536781641507E-5	0.13315641047916846	4.239907356328301E-6
62-63	0.0019736768743708246	2.755939781613396E-4	4.239907356328301E-6	2.1199536781641507E-5	0.13503044953066556	8.479814712656603E-6
64-65	0.0020075961332214507	2.9255360758665284E-4	4.239907356328301E-6	2.1199536781641507E-5	0.13670945284377156	8.479814712656603E-6
66-67	0.0021072339560951657	3.285928201154434E-4	4.239907356328301E-6	2.1199536781641507E-5	0.13804078375365866	8.479814712656603E-6
68-69	0.002141153214945792	3.413125421844283E-4	4.239907356328301E-6	2.1199536781641507E-5	0.13940815387607455	8.479814712656603E-6
70-71	0.002162352751727434	3.476724032189207E-4	4.239907356328301E-6	2.1199536781641507E-5	0.14076280427642143	8.479814712656603E-6
72-73	0.0021983919642562244	3.6675198632239806E-4	4.239907356328301E-6	2.1199536781641507E-5	0.14182702102285982	8.479814712656603E-6
74-75	0.002208991732647045	3.7523180103505465E-4	6.359861034492452E-6	2.1199536781641507E-5	0.1428530786030913	8.479814712656603E-6
76-77	0.0022238314083941942	3.8583156942587543E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.14413353062470244	8.479814712656603E-6
78-79	0.0022916699260954465	4.006712451730245E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.14575741514217616	8.479814712656603E-6
80-81	0.002293789879773611	4.1551092092017357E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.14702514744171835	8.479814712656603E-6
82-83	0.0023149894165552525	4.2823064298915843E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.1486172326540196	1.4839675747149055E-5
84-85	0.002353148582762207	4.2823064298915843E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.15016903874643578	1.9079583103477356E-5
86-87	0.002376468073222013	4.3035059666732256E-4	1.4839675747149055E-5	2.3319490459805657E-5	0.15189044113310507	2.1199536781641507E-5
88-89	0.0023997875636818187	4.3247055034548675E-4	1.9079583103477356E-5	2.5439444137969808E-5	0.15354612495575126	2.1199536781641507E-5
90-91	0.0024103873320726393	4.4095036505814334E-4	2.1199536781641507E-5	2.5439444137969808E-5	0.15456370272127007	2.1199536781641507E-5
92-93	0.0024167471931071318	4.4519027241447166E-4	2.1199536781641507E-5	2.5439444137969808E-5	0.15552828164483473	2.1199536781641507E-5
94-95	0.0024252270078197885	4.4519027241447166E-4	2.1199536781641507E-5	2.5439444137969808E-5	0.15657553876184782	2.1199536781641507E-5
96-97	0.0024252270078197885	4.515501334489641E-4	2.1199536781641507E-5	2.5439444137969808E-5	0.15740232069633187	2.1199536781641507E-5
98-99	0.002433706822532445	4.5367008712712825E-4	2.1199536781641507E-5	3.1799305172462263E-5	0.15824182235288486	2.1199536781641507E-5
100-101	0.002435826776210609	4.579099944834565E-4	2.1199536781641507E-5	3.391925885062641E-5	0.1590092455843803	3.1799305172462263E-5
102-103	0.0024379467298887734	4.6214990183978484E-4	2.3319490459805657E-5	3.603921252879056E-5	0.15993354538805984	3.391925885062641E-5
104-105	0.0024421866372451016	4.6214990183978484E-4	2.5439444137969808E-5	4.2399073563283013E-5	0.16079000667403817	3.391925885062641E-5
106-107	0.0024803458034520563	4.6638980919611316E-4	2.5439444137969808E-5	4.2399073563283013E-5	0.16156802967392442	3.603921252879056E-5
108-109	0.002488825618164713	4.79109531265098E-4	2.5439444137969808E-5	4.2399073563283013E-5	0.16236937216427044	3.815916620695471E-5
110-111	0.0025100251549463543	4.93949207012247E-4	2.5439444137969808E-5	4.2399073563283013E-5	0.1632957919216282	3.815916620695471E-5
112-113	0.0025291047380498317	5.045489754030678E-4	2.967935149429811E-5	4.2399073563283013E-5	0.16411197408772138	3.815916620695471E-5
114-115	0.0025375845527624884	5.130287901157245E-4	2.967935149429811E-5	4.2399073563283013E-5	0.1648857571802513	3.815916620695471E-5
116-117	0.0025397045064406523	5.172686974720528E-4	2.967935149429811E-5	4.2399073563283013E-5	0.16578885744714925	3.815916620695471E-5
118-119	0.002552424228509637	5.172686974720528E-4	3.391925885062641E-5	4.2399073563283013E-5	0.16656900040071365	4.875893459777547E-5
120-121	0.0025545441821878015	5.172686974720528E-4	3.391925885062641E-5	4.451902724144716E-5	0.16749118025071505	5.511879563226792E-5
122-123	0.002560904043222294	5.172686974720528E-4	3.391925885062641E-5	4.6638980919611315E-5	0.16820560464025636	5.935870298859622E-5
124-125	0.0025693838579349502	5.193886511502168E-4	3.391925885062641E-5	4.6638980919611315E-5	0.16912778449025778	6.147865666676037E-5
126-127	0.002590583394716592	5.257485121847094E-4	4.0279119885118866E-5	4.6638980919611315E-5	0.17021744068083414	6.783851770125282E-5
128-129	0.0026075430241419054	5.384682342536943E-4	4.2399073563283013E-5	4.6638980919611315E-5	0.1713092168250887	6.783851770125282E-5
130-131	0.002613902885176398	5.49068002644515E-4	4.2399073563283013E-5	4.6638980919611315E-5	0.17224411639715909	6.995847137941697E-5
132-133	0.002630862514601711	5.533079100008434E-4	4.6638980919611315E-5	4.6638980919611315E-5	0.1732192950891146	7.843828609207357E-5
134-135	0.0026563019587396808	5.554278636790075E-4	4.6638980919611315E-5	4.6638980919611315E-5	0.17404183711624227	8.479814712656603E-5
136-137	0.002666901727130502	5.575478173571716E-4	4.875893459777547E-5	4.6638980919611315E-5	0.17492373784635856	8.479814712656603E-5
138-139	0.0026902212175903073	5.723874931043207E-4	5.0878888275939616E-5	4.6638980919611315E-5	0.17575687964187708	8.691810080473017E-5
>>END_MODULE
