##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22805127
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.645550011626774	37.0	35.0	38.0	32.0	39.0
2	35.203685907997794	35.0	34.0	37.0	33.0	39.0
3	35.38664568717377	35.0	34.0	37.0	33.0	39.0
4	35.431906825162606	36.0	35.0	37.0	33.0	39.0
5	35.34332064890496	35.0	34.0	37.0	33.0	39.0
6	35.29991330458278	35.0	34.0	37.0	33.0	39.0
7	35.339814332101724	35.0	34.0	37.0	33.0	39.0
8	35.40714173615433	36.0	34.0	38.0	33.0	39.0
9	35.41620219874241	36.0	34.0	38.0	33.0	39.0
10-14	35.40640376175059	35.4	34.2	37.6	33.0	39.0
15-19	35.37778867006529	36.0	34.0	37.6	33.0	39.0
20-24	35.36315345229167	36.0	34.0	37.8	33.0	39.0
25-29	35.33936194260176	36.0	34.0	37.6	33.0	39.0
30-34	35.32316060331521	36.0	34.0	37.6	32.6	39.0
35-39	35.29268941146436	35.8	34.0	37.0	32.0	39.0
40-44	35.274346281868986	35.8	34.0	37.0	32.0	39.0
45-49	35.24164453019709	35.4	34.0	37.0	32.0	39.0
50-54	35.21830246330134	35.2	34.0	37.0	32.0	39.0
55-59	35.189451363283354	35.0	34.0	37.0	32.0	39.0
60-64	35.146577197311814	35.0	34.0	37.0	32.0	39.0
65-69	35.11634335559719	35.0	34.0	37.0	32.0	39.0
70-74	35.085217740729966	35.0	34.0	37.0	32.0	39.0
75-79	35.03634566034208	35.0	34.0	37.0	32.0	39.0
80-84	34.991900873869284	35.0	34.0	37.0	32.0	39.0
85-89	34.94042888689022	35.0	34.0	37.0	31.2	39.0
90-94	34.88271015548389	35.0	34.0	37.0	31.0	39.0
95-99	34.81664366964499	35.0	34.0	37.0	31.0	39.0
100-104	34.76364280716349	35.0	34.0	37.0	31.0	39.0
105-109	34.70131388437345	35.0	34.0	37.0	31.0	39.0
110-114	34.622883634894905	35.0	34.0	37.0	30.0	39.0
115-119	34.55814630631086	35.0	34.0	37.0	30.0	39.0
120-124	34.480639226433595	35.0	34.0	37.0	30.0	39.0
125-129	34.39999040566623	35.0	34.0	37.0	30.0	39.0
130-134	34.29615015079723	35.0	34.0	37.0	29.8	39.0
135-139	34.23279292415254	35.0	34.0	37.0	29.0	39.0
140-144	34.10020149416401	35.0	34.0	37.0	29.0	39.0
145-149	34.021160890706724	35.0	34.0	37.0	28.6	39.0
150	33.95131178177609	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	13.0
21	285.0
22	2211.0
23	10581.0
24	34300.0
25	80344.0
26	156491.0
27	261835.0
28	380159.0
29	500047.0
30	643160.0
31	836893.0
32	1121160.0
33	1560315.0
34	2336761.0
35	3955135.0
36	9541434.0
37	1383990.0
38	11.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.1428937010524	19.083756911329633	14.204301515181214	33.569047872436755
2	23.780801571506267	26.122630231351046	34.272385328088724	15.824182869053963
3	18.89229557897222	30.040450991568697	30.075403658133542	20.991849771325544
4	22.71009058620897	34.574940100092405	22.62463173303091	20.090337580667715
5	24.663028625098207	35.510422722048425	22.808721915909523	17.017826736943846
6	18.307032449325977	39.0148101345807	23.550897129404277	19.12726028668904
7	19.625428966039085	20.520933735646373	38.81667047940579	21.03696681890875
8	20.75022173069218	23.17718449894483	29.244889943302006	26.82770382706099
9	23.004730471354094	23.10648829098825	29.46288349983756	24.425897737820097
10-14	22.856483105750737	29.177929156018294	25.91497429503462	22.050613443196347
15-19	22.70801473721238	28.38087856296525	27.351729284384163	21.559377415438206
20-24	22.83547028701046	28.419551445602558	27.197438540903544	21.547539726483436
25-29	22.90754530768454	28.43009030381633	27.14327615890935	21.519088229589777
30-34	22.970839171128834	28.17181266451016	27.251390682905562	21.605957481455444
35-39	23.057376527655382	28.151764294055454	27.248987475491802	21.54187170279736
40-44	23.223172578692502	27.902593131798824	27.348028362218724	21.526205927289947
45-49	23.245592098653955	27.714718712156262	27.40954084579314	21.63014834339664
50-54	23.31729965809881	27.66620944492	27.4080192581256	21.608471638855594
55-59	23.489962586044797	27.562136356443006	27.278034452515875	21.669866604996322
60-64	23.43112844756357	27.521546361044162	27.33892865406976	21.708396537322507
65-69	23.519970750436954	27.388525396065543	27.3177413131705	21.773762540327006
70-74	23.553187842365446	27.38931995423661	27.216311489955746	21.841180713442203
75-79	23.515566477660922	27.437423172429604	27.264865483976475	21.782144865933
80-84	23.62057795161588	27.493084340201218	27.17445862064263	21.71187908754027
85-89	23.666378178906875	27.388113208051855	27.212340453091972	21.733168159949294
90-94	23.577571832860215	27.33595388440503	27.276521634806066	21.80995264792869
95-99	23.697492234969793	27.461173094979912	27.222236473403544	21.619098196646746
100-104	23.827365871081234	27.46668525429992	27.019024511628675	21.68692436299017
105-109	23.6208182484579	27.6036752612691	27.099295697848998	21.676210792424
110-114	23.63900475159762	27.58286095153948	27.053754922779937	21.72437937408296
115-119	23.741229768200807	27.59805459535481	27.04743718375258	21.6132784526918
120-124	23.721184275799033	27.47923043796248	27.247386081208845	21.55219920502964
125-129	23.684308565212625	27.555007499454025	27.211138330526623	21.54954560480672
130-134	23.730108139841875	27.471055796487327	27.280269245384847	21.51856681828595
135-139	23.673490614632403	27.488808985803935	27.36494648769112	21.472753911872537
140-144	23.700454726693696	27.628360938310053	27.338443675406847	21.3327406595894
145-149	23.80192839969714	28.11049550392769	26.888673761825576	21.198902334549594
150	23.436988533324108	27.994318996776467	27.049171881393164	21.51952058850626
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.0
8	4.0
9	2.5
10	3.5
11	6.0
12	11.0
13	20.5
14	37.0
15	50.0
16	96.5
17	174.5
18	260.0
19	459.5
20	731.0
21	1090.0
22	1697.5
23	2892.0
24	4736.0
25	7575.0
26	12006.5
27	19195.0
28	31526.0
29	48060.0
30	64034.0
31	82901.5
32	107253.5
33	138366.0
34	185474.5
35	254450.0
36	350450.5
37	471367.5
38	613702.0
39	790814.5
40	995446.0
41	1180346.5
42	1348423.0
43	1504554.0
44	1607699.5
45	1648544.0
46	1638824.0
47	1579423.0
48	1473111.5
49	1328973.0
50	1151582.5
51	961948.0
52	782739.5
53	621404.5
54	483801.5
55	367778.0
56	273515.0
57	201537.5
58	145912.5
59	104327.0
60	71298.5
61	46542.0
62	30916.0
63	21108.0
64	14135.0
65	9293.0
66	6555.5
67	4900.5
68	4065.0
69	2882.0
70	1527.5
71	932.5
72	518.5
73	345.0
74	300.5
75	242.5
76	148.0
77	52.5
78	19.5
79	2.0
80	0.5
81	0.0
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.1156231228179523
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	8.769957738012158E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.769957738012158E-7
105-109	0.0
110-114	0.018814190335357486
115-119	0.0
120-124	0.0
125-129	8.769957738012158E-7
130-134	0.04715167777842237
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2805127E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.875905806401484
#Duplication Level	Percentage of total
1	29.493942027078866
2	10.672239278598694
3	7.0222540183290425
4	5.15051635637501
5	3.990566537205949
6	3.0474657593786607
7	2.680242083115881
8	2.186058494739721
9	1.8542031428499017
>10	20.586740205669713
>50	4.9833245125148675
>100	6.101368472028455
>500	1.0516400422785195
>1k	0.9423183410606397
>5k	0.11540518021408877
>10k+	0.12171554856175483
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	8.769957738012158E-6	4.384978869006079E-6	0.0	5.393524008877477E-4	0.0
2	0.0	3.069485208304255E-5	4.384978869006079E-6	0.0	0.004845401650251717	0.0
3	3.507983095204863E-5	3.507983095204863E-5	4.384978869006079E-6	0.0	0.006353834381189809	0.0
4	7.454464077310334E-5	3.946480982105471E-5	4.384978869006079E-6	0.0	0.007875422048734919	0.0
5	8.33145985111155E-5	4.384978869006079E-5	4.384978869006079E-6	0.0	0.009620643638599338	0.0
6	1.052394928561459E-4	4.823476755906687E-5	4.384978869006079E-6	4.384978869006079E-6	0.011510569531140958	0.0
7	1.227794083321702E-4	7.454464077310334E-5	4.384978869006079E-6	4.384978869006079E-6	0.013795143521893125	0.0
8	1.4908928154620669E-4	7.454464077310334E-5	4.384978869006079E-6	4.384978869006079E-6	0.01971486499505133	0.0
9	1.6224421815322492E-4	7.892961964210942E-5	4.384978869006079E-6	4.384978869006079E-6	0.026296718277429458	0.0
10-11	1.8197662306375226E-4	8.33145985111155E-5	4.384978869006079E-6	4.384978869006079E-6	0.033442041344474864	0.0
12-13	2.060940068432857E-4	8.33145985111155E-5	4.384978869006079E-6	4.384978869006079E-6	0.03976737336301613	0.0
14-15	2.477513060988435E-4	8.550708794561854E-5	4.384978869006079E-6	4.384978869006079E-6	0.04931785733971138	0.0
16-17	2.894086053544012E-4	9.208455624912766E-5	4.384978869006079E-6	4.384978869006079E-6	0.05540201552045731	2.1924894345030395E-6
18-19	3.5299079895498937E-4	9.866202455263678E-5	4.384978869006079E-6	4.384978869006079E-6	0.06329278499523375	4.384978869006079E-6
20-21	3.924556087760441E-4	1.0962447172515197E-4	4.384978869006079E-6	4.384978869006079E-6	0.06980447861570777	4.384978869006079E-6
22-23	4.384978869006079E-4	1.271643872011763E-4	6.5774683035091185E-6	4.384978869006079E-6	0.07670424286608885	4.384978869006079E-6
24-25	4.933101227631839E-4	1.2935687663567932E-4	8.769957738012158E-6	4.384978869006079E-6	0.08229289843463708	4.384978869006079E-6
26-27	5.437373797567538E-4	1.3154936607018237E-4	8.769957738012158E-6	4.384978869006079E-6	0.0866296425360841	4.384978869006079E-6
28-29	6.555543409164089E-4	1.3374185550468542E-4	8.769957738012158E-6	4.384978869006079E-6	0.09137638216178318	4.384978869006079E-6
30-31	8.638408371941976E-4	1.4031932380819453E-4	8.769957738012158E-6	4.384978869006079E-6	0.09663835680459049	4.384978869006079E-6
32-33	0.00104581746025795	1.6443670758772795E-4	8.769957738012158E-6	4.384978869006079E-6	0.1022818246090013	4.384978869006079E-6
34-35	0.0011861367840661444	1.7539915476024316E-4	8.769957738012158E-6	4.384978869006079E-6	0.10586873732384827	4.384978869006079E-6
36-37	0.0012277940833217023	1.9293907023626748E-4	8.769957738012158E-6	4.384978869006079E-6	0.10946003501756425	4.384978869006079E-6
38-39	0.001265066403708254	1.9293907023626748E-4	8.769957738012158E-6	4.384978869006079E-6	0.11293293828181707	4.384978869006079E-6
40-41	0.0012913762769222903	1.9951653853977658E-4	8.769957738012158E-6	4.384978869006079E-6	0.11604846576824589	4.384978869006079E-6
42-43	0.0013045312135293087	2.1267147514679484E-4	8.769957738012158E-6	4.384978869006079E-6	0.11914206836032967	4.384978869006079E-6
44-45	0.001326456107874339	2.324038800573222E-4	8.769957738012158E-6	4.384978869006079E-6	0.12161300395301461	4.384978869006079E-6
46-47	0.0013549584705228785	2.543287744023526E-4	8.769957738012158E-6	4.384978869006079E-6	0.12433388334123288	4.384978869006079E-6
48-49	0.0013922307909094301	2.76253668747383E-4	8.769957738012158E-6	4.384978869006079E-6	0.12670177193049614	4.384978869006079E-6
50-51	0.001429503111295982	2.8502362648539516E-4	8.769957738012158E-6	4.384978869006079E-6	0.12893811115368925	6.5774683035091185E-6
52-53	0.00149308530489657	2.8502362648539516E-4	8.769957738012158E-6	4.384978869006079E-6	0.13114594801423382	8.769957738012158E-6
54-55	0.0015566674984971581	3.003710525269164E-4	8.769957738012158E-6	4.384978869006079E-6	0.13320469559323217	8.769957738012158E-6
56-57	0.0016290196498357583	3.1352598913393463E-4	1.3154936607018237E-5	4.384978869006079E-6	0.13532702536583113	8.769957738012158E-6
58-59	0.0016882168645673404	3.3545088347896506E-4	1.3154936607018237E-5	4.384978869006079E-6	0.13736604053991894	8.769957738012158E-6
60-61	0.0017671464842094501	3.551832883894924E-4	1.3154936607018237E-5	4.384978869006079E-6	0.1390542574044863	8.769957738012158E-6
62-63	0.0018373061461135472	3.639532461275046E-4	1.3154936607018237E-5	4.384978869006079E-6	0.14121385949747178	8.769957738012158E-6
64-65	0.0018614235298930807	3.749156933000198E-4	1.3154936607018237E-5	4.384978869006079E-6	0.14302924074924028	8.769957738012158E-6
66-67	0.001938160660100687	3.924556087760441E-4	1.3154936607018237E-5	4.384978869006079E-6	0.14439735415637017	1.3154936607018237E-5
68-69	0.0019557005755767116	4.3411290803160185E-4	1.3154936607018237E-5	4.384978869006079E-6	0.14570188536989948	1.3154936607018237E-5
70-71	0.001984202938225251	4.4069037633511095E-4	1.3154936607018237E-5	4.384978869006079E-6	0.14698449168908378	1.3154936607018237E-5
72-73	0.002008320322004784	4.691927389836505E-4	1.3154936607018237E-5	4.384978869006079E-6	0.1478680649311885	1.7539915476024316E-5
74-75	0.0020170902797427962	4.7357771785265653E-4	1.3154936607018237E-5	4.384978869006079E-6	0.148942384754095	1.7539915476024316E-5
76-77	0.002043400152956833	4.889251438941778E-4	1.5347426041521276E-5	4.384978869006079E-6	0.15036750288652195	1.7539915476024316E-5
78-79	0.0020916349205159	5.086575488047052E-4	1.9732404910527355E-5	4.384978869006079E-6	0.1521061470080829	1.7539915476024316E-5
80-81	0.0021025973676884148	5.196199959772204E-4	2.1924894345030395E-5	4.384978869006079E-6	0.15361457973902098	1.7539915476024316E-5
82-83	0.0021223297725989423	5.371599114532446E-4	2.1924894345030395E-5	4.384978869006079E-6	0.15544969339570003	1.7539915476024316E-5
84-85	0.002155217114116488	5.437373797567538E-4	2.1924894345030395E-5	4.384978869006079E-6	0.15707871304553578	1.7539915476024316E-5
86-87	0.0021859119661995306	5.546998269292691E-4	2.6309873214036474E-5	4.384978869006079E-6	0.159040991089416	1.7539915476024316E-5
88-89	0.0021968744133720455	5.612772952327781E-4	3.288734151754559E-5	4.384978869006079E-6	0.16102957900651024	2.1924894345030395E-5
90-91	0.002205644371110058	5.832021895778085E-4	3.507983095204863E-5	4.384978869006079E-6	0.1621499411075413	2.1924894345030395E-5
92-93	0.002210029349979064	5.832021895778085E-4	3.946480982105471E-5	4.384978869006079E-6	0.16318698861006126	2.4117383779533434E-5
94-95	0.002223184286586082	5.897796578813177E-4	3.946480982105471E-5	4.384978869006079E-6	0.16427446336957474	2.6309873214036474E-5
96-97	0.002223184286586082	5.985496156193298E-4	3.946480982105471E-5	4.384978869006079E-6	0.16534439821361224	2.6309873214036474E-5
98-99	0.0022407242020621066	6.007421050538328E-4	3.946480982105471E-5	4.384978869006079E-6	0.16626962875497253	2.6309873214036474E-5
100-101	0.0022451091809311124	6.07319573357342E-4	3.946480982105471E-5	4.384978869006079E-6	0.16707427237743513	2.6309873214036474E-5
102-103	0.002256071628103628	6.182820205298573E-4	3.946480982105471E-5	6.5774683035091185E-6	0.1679710005561469	2.6309873214036474E-5
104-105	0.002258264117538131	6.314369571368754E-4	3.946480982105471E-5	8.769957738012158E-6	0.16886992122429312	2.6309873214036474E-5
106-107	0.002288958969621173	6.445918937438937E-4	3.946480982105471E-5	8.769957738012158E-6	0.16964167750523818	3.288734151754559E-5
108-109	0.0023021139062281916	6.533618514819058E-4	4.384978869006079E-5	8.769957738012158E-6	0.1705120958107359	3.507983095204863E-5
110-111	0.002317461332269713	6.730942563924332E-4	4.384978869006079E-5	8.769957738012158E-6	0.1714395188415307	3.507983095204863E-5
112-113	0.002324038800573222	6.840567035649483E-4	4.384978869006079E-5	8.769957738012158E-6	0.17222004508021377	3.507983095204863E-5
114-115	0.002326231290007725	6.928266613029604E-4	4.384978869006079E-5	1.3154936607018237E-5	0.1730334586604144	3.507983095204863E-5
116-117	0.0023284237794422282	7.015966190409726E-4	4.384978869006079E-5	1.3154936607018237E-5	0.17400034650103022	3.727232038655167E-5
118-119	0.002332808758311234	7.081740873444818E-4	4.384978869006079E-5	1.3154936607018237E-5	0.17486418733822442	4.823476755906687E-5
120-121	0.00233719373718024	7.103665767789849E-4	4.384978869006079E-5	1.3154936607018237E-5	0.17582888268940577	5.261974642807295E-5
122-123	0.00233719373718024	7.169440450824939E-4	4.384978869006079E-5	1.3154936607018237E-5	0.17663571880130288	5.261974642807295E-5
124-125	0.0023525411632217613	7.257140028205061E-4	4.823476755906687E-5	1.3154936607018237E-5	0.17760041415248423	5.261974642807295E-5
126-127	0.00236569609982878	7.366764499930213E-4	4.823476755906687E-5	1.3154936607018237E-5	0.17868131144369423	6.577468303509118E-5
128-129	0.002385428504739307	7.432539182965304E-4	4.823476755906687E-5	1.3154936607018237E-5	0.17973589886169017	6.577468303509118E-5
130-131	0.0023963909519118223	7.498313866000396E-4	4.823476755906687E-5	1.3154936607018237E-5	0.18086503092045925	6.577468303509118E-5
132-133	0.0024139308673878463	7.564088549035486E-4	4.823476755906687E-5	1.3154936607018237E-5	0.18179464644068855	6.577468303509118E-5
134-135	0.0024380482511673802	7.586013443380517E-4	5.261974642807295E-5	1.3154936607018237E-5	0.18264313985184122	6.577468303509118E-5
136-137	0.002449010698339895	7.651788126415609E-4	5.261974642807295E-5	1.3154936607018237E-5	0.18355302296715997	7.454464077310334E-5
138-139	0.002481898039857441	7.76141259814076E-4	5.261974642807295E-5	1.3154936607018237E-5	0.18438836144170562	8.112210907661246E-5
>>END_MODULE
