##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.S.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24144766
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.6762389413921	37.0	35.0	38.0	32.0	39.0
2	35.26436189110302	35.0	34.0	37.0	33.0	39.0
3	35.42898332499888	35.0	34.0	37.0	33.0	39.0
4	35.47995035445777	36.0	35.0	37.0	33.0	39.0
5	35.40000946789047	36.0	35.0	37.0	33.0	39.0
6	35.35822695486053	35.0	34.0	37.0	33.0	39.0
7	35.403993436921276	35.0	34.0	37.0	33.0	39.0
8	35.49334899331806	36.0	34.0	38.0	33.0	39.0
9	35.47122357698559	36.0	34.0	38.0	33.0	39.0
10-14	35.44925971947709	35.8	34.4	37.8	33.0	39.0
15-19	35.42736877218027	36.0	34.0	38.0	33.0	39.0
20-24	35.413720116401215	36.0	34.0	38.0	33.0	39.0
25-29	35.396267895079205	36.0	34.0	38.0	33.0	39.0
30-34	35.37821687731411	36.0	34.0	38.0	32.8	39.0
35-39	35.347688480393636	36.0	34.0	38.0	32.4	39.0
40-44	35.33214183148431	36.0	34.0	38.0	32.0	39.0
45-49	35.30147117598903	36.0	34.0	38.0	32.0	39.0
50-54	35.27938478260672	36.0	34.0	38.0	32.0	39.0
55-59	35.25330047100063	36.0	34.0	38.0	32.0	39.0
60-64	35.20921463475769	36.0	34.0	38.0	32.0	39.0
65-69	35.18132159988629	36.0	34.0	38.0	32.0	39.0
70-74	35.14522428587629	36.0	34.0	38.0	32.0	39.0
75-79	35.09545873420351	35.4	34.0	37.8	32.0	39.0
80-84	35.05166494469236	35.2	34.0	37.6	32.0	39.0
85-89	34.99939995276823	35.0	34.0	37.2	32.0	39.0
90-94	34.94462643373724	35.0	34.0	37.0	31.0	39.0
95-99	34.87608651084049	35.0	34.0	37.0	31.0	39.0
100-104	34.82365729284765	35.0	34.0	37.0	31.0	39.0
105-109	34.76404772777669	35.0	34.0	37.0	31.0	39.0
110-114	34.685153237765896	35.0	34.0	37.0	30.4	39.0
115-119	34.605448153856614	35.0	34.0	37.0	30.0	39.0
120-124	34.52932635586529	35.0	34.0	37.0	30.0	39.0
125-129	34.444939387691726	35.0	34.0	37.0	30.0	39.0
130-134	34.33239009232891	35.0	34.0	37.0	29.8	39.0
135-139	34.268480580843075	35.0	34.0	37.0	29.0	39.0
140-144	34.135924622338436	35.0	34.0	37.0	29.0	39.0
145-149	34.045551222157215	35.0	34.0	37.0	28.6	39.0
150	33.97768551577597	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	17.0
21	300.0
22	2463.0
23	11912.0
24	38301.0
25	89056.0
26	167520.0
27	275528.0
28	396716.0
29	517546.0
30	663996.0
31	865410.0
32	1155865.0
33	1606017.0
34	2400908.0
35	4033045.0
36	9908986.0
37	2011178.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.99317268181436	18.987912328493888	14.919726287676593	32.09918870201517
2	23.862306224048723	26.419634797868824	32.757422457521436	16.96063652056102
3	19.54862184210027	30.068665813534906	29.50049712637513	20.88221521798969
4	23.004753908155497	34.86410677991247	21.654386710560793	20.476752601371246
5	24.935669287496925	35.42883786904375	21.84849503200818	17.786997811451144
6	18.806804754289193	39.37952432423656	22.08881212599037	19.724858795483875
7	20.291776693963403	20.715379888129792	37.17997515486379	21.812868263043015
8	21.410727277290658	23.490196591675396	28.2300188786257	26.869057252408247
9	23.45867009023819	23.4949926621778	27.84325182526101	25.203085422323003
10-14	23.018044573304213	28.85913659299908	25.321764559656536	22.801054274040176
15-19	22.97381718257282	27.759798541845466	26.902762279824955	22.36362199575676
20-24	22.902251361640865	27.719668105294538	26.810813573426223	22.567266959638374
25-29	22.97213151703355	27.36666240625401	26.781570796751563	22.879635279960883
30-34	22.894440334558972	27.060970705294647	26.97663689908311	23.06795206106327
35-39	23.17725506223585	26.792011154715684	27.054801028098595	22.97593275494987
40-44	23.37031305252658	26.407971814678177	27.08291478161354	23.1388003511817
45-49	23.256455664138556	26.278746292260607	27.127199327589256	23.33759871601158
50-54	23.513252520235646	26.30670514678005	26.808633390772975	23.37140894221133
55-59	23.540327539310177	26.330720289440784	26.80447431132694	23.324477859922105
60-64	23.25029863615162	26.30687661251304	26.90348955960062	23.53933519173472
65-69	23.491647204132335	26.240291798564474	26.86151546767126	23.406545529631934
70-74	23.637214790153692	26.199151402005715	26.597543335064834	23.566090472775755
75-79	23.44441441263088	26.333704787199014	26.841957383227488	23.37992341694262
80-84	23.74335641723226	26.317930986102173	26.5522699748034	23.38644262186217
85-89	23.492764435985837	26.390429296353503	26.75563474087925	23.361171526781416
90-94	23.52386868047401	26.142210913472603	26.781233885482543	23.552686520570848
95-99	23.558810219987222	26.29187791672945	26.767716862528303	23.381595000755027
100-104	23.76364798896788	26.134709278193046	26.719530849874463	23.382111882964615
105-109	23.587010120537098	26.33813224779234	26.590168651872624	23.48468897979794
110-114	23.667242830185227	26.42399184982783	26.483498742543212	23.42526657744374
115-119	23.83226990064845	26.506796545470767	26.640318651255512	23.020614902625272
120-124	23.789519434563996	26.64997623087339	26.763053325925796	22.79745100863682
125-129	23.75330971319672	26.728651888601046	26.590934255407024	22.927104142795205
130-134	23.901593971951183	26.741232284049442	26.611095582996004	22.74607816100337
135-139	23.656841404054198	26.751657067208683	26.900680669259746	22.69082085947737
140-144	23.713623068453014	26.861082024982142	26.679167650661846	22.746127255902998
145-149	23.912822348330067	27.463268850897126	26.110598876791762	22.51330992398104
150	23.68819809643216	27.485658796610412	26.057813109474743	22.768329997482684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	2.0
11	3.0
12	2.5
13	6.5
14	17.5
15	25.0
16	52.0
17	100.0
18	178.0
19	329.0
20	502.5
21	693.5
22	1066.0
23	1836.5
24	3009.5
25	4712.0
26	7223.5
27	11381.0
28	17472.5
29	24679.0
30	34857.0
31	50553.5
32	72513.0
33	99759.0
34	135324.0
35	191745.0
36	272565.0
37	368240.5
38	487268.5
39	634466.0
40	808844.0
41	996916.0
42	1187603.5
43	1381551.5
44	1532423.5
45	1623769.0
46	1649050.5
47	1613103.5
48	1561341.5
49	1502721.5
50	1388886.0
51	1223613.5
52	1034609.5
53	863380.5
54	733241.0
55	628256.0
56	511181.0
57	392242.5
58	319006.0
59	258662.5
60	190472.5
61	126721.0
62	80005.0
63	49606.5
64	30412.5
65	15222.5
66	8313.5
67	4892.5
68	3318.0
69	2156.5
70	919.5
71	584.0
72	358.0
73	263.0
74	238.5
75	149.5
76	89.0
77	36.5
78	14.5
79	3.0
80	1.5
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	8.28336874335415E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.28336874335415E-7
70-74	0.0
75-79	0.0
80-84	8.28336874335415E-7
85-89	0.0
90-94	8.28336874335415E-7
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	8.28336874335415E-7
130-134	0.05347328692272271
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4144766E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.11153621206665
#Duplication Level	Percentage of total
1	25.583714267298475
2	8.383190157529121
3	5.25235333240848
4	3.787029286954803
5	2.8229912120431
6	2.2414340840953355
7	1.8315949005239303
8	1.5624848929952662
9	1.3797832954541802
>10	17.466029765040506
>50	6.255397742072745
>100	12.3997582273933
>500	3.759581783861992
>1k	5.446329654892843
>5k	1.0118045260489277
>10k+	0.8165228713868946
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	34680	0.14363361400976096	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	26216	0.1085783974878862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.3193432481391618E-4	4.141684371677075E-6	0.0	0.0	0.0017229406986176631	0.0
2	0.001433022792600268	4.141684371677075E-6	0.0	0.0	0.008904621399105712	0.0
3	0.0021122590295553083	4.141684371677075E-6	0.0	0.0	0.013087722614499557	0.0
4	0.005214380623941437	8.28336874335415E-6	0.0	4.141684371677075E-6	0.019610875499890948	0.0
5	0.005404898105038583	8.28336874335415E-6	0.0	4.141684371677075E-6	0.023288691221940192	0.0
6	0.006398902354241081	8.28336874335415E-6	0.0	4.141684371677075E-6	0.031157891528126633	0.0
7	0.008304077165212535	1.65667374867083E-5	0.0	4.141684371677075E-6	0.04083700790473596	0.0
8	0.015183414906568157	1.65667374867083E-5	0.0	4.141684371677075E-6	0.05527906130877392	0.0
9	0.015783959140461334	1.65667374867083E-5	0.0	4.141684371677075E-6	0.06511556169150697	0.0
10-11	0.01648390379927476	1.863757967254684E-5	0.0	4.141684371677075E-6	0.1109640076859722	0.0
12-13	0.017210769406504085	2.0708421858385375E-5	0.0	4.141684371677075E-6	0.13105738941516354	0.0
14-15	0.020223844786899155	3.106263278757806E-5	4.141684371677075E-6	4.141684371677075E-6	0.14184233551901063	0.0
16-17	0.02373185144970964	3.31334749734166E-5	4.141684371677075E-6	4.141684371677075E-6	0.1497301734048696	0.0
18-19	0.030551134767675944	3.5204317159255134E-5	4.141684371677075E-6	4.141684371677075E-6	0.15683730378666746	0.0
20-21	0.036113416878838255	3.727515934509367E-5	4.141684371677075E-6	4.141684371677075E-6	0.16180732503267997	2.0708421858385374E-6
22-23	0.03845139770664996	4.97002124601249E-5	4.141684371677075E-6	4.141684371677075E-6	0.16716459376744425	4.141684371677075E-6
24-25	0.0441172219271042	4.97002124601249E-5	4.141684371677075E-6	4.141684371677075E-6	0.17180328026372257	4.141684371677075E-6
26-27	0.049118305805904275	4.97002124601249E-5	4.141684371677075E-6	4.141684371677075E-6	0.17562605493878053	4.141684371677075E-6
28-29	0.061249299330546424	5.384189683180197E-5	4.141684371677075E-6	4.141684371677075E-6	0.17911542402191846	4.141684371677075E-6
30-31	0.08561275764693682	5.384189683180197E-5	4.141684371677075E-6	4.141684371677075E-6	0.18329438355294064	4.141684371677075E-6
32-33	0.1055528970543761	5.7983581203479045E-5	4.141684371677075E-6	4.141684371677075E-6	0.186483480519132	4.141684371677075E-6
34-35	0.11966154486649405	6.62669499468332E-5	4.141684371677075E-6	4.141684371677075E-6	0.18942200558083686	4.141684371677075E-6
36-37	0.12184007084599618	6.62669499468332E-5	4.141684371677075E-6	4.141684371677075E-6	0.19149491860886123	4.141684371677075E-6
38-39	0.12389641713653385	6.833779213267173E-5	4.141684371677075E-6	4.141684371677075E-6	0.19319508004343466	4.141684371677075E-6
40-41	0.12532943992913412	7.040863431851027E-5	4.141684371677075E-6	4.141684371677075E-6	0.19500913779822923	4.141684371677075E-6
42-43	0.12559864941329313	7.040863431851027E-5	4.141684371677075E-6	4.141684371677075E-6	0.1969308793466874	4.141684371677075E-6
44-45	0.1264891115532037	7.455031869018734E-5	4.141684371677075E-6	4.141684371677075E-6	0.1984032481408186	4.141684371677075E-6
46-47	0.12789521339738807	8.904621399105711E-5	4.141684371677075E-6	4.141684371677075E-6	0.19993774220052496	4.141684371677075E-6
48-49	0.12907973512768772	9.318789836273418E-5	4.141684371677075E-6	4.141684371677075E-6	0.2011657516167272	4.141684371677075E-6
50-51	0.13014207716902287	9.94004249202498E-5	4.141684371677075E-6	4.141684371677075E-6	0.20245174461413293	4.141684371677075E-6
52-53	0.13135559068992428	9.94004249202498E-5	4.141684371677075E-6	4.141684371677075E-6	0.20376258771776873	6.212526557515612E-6
54-55	0.13196234745037494	1.2010884677863518E-4	6.212526557515612E-6	4.141684371677075E-6	0.2049761012386701	8.28336874335415E-6
56-57	0.1327554800075511	1.2839221552198932E-4	8.28336874335415E-6	4.141684371677075E-6	0.20617097717989896	8.28336874335415E-6
58-59	0.13292735990897572	1.3460474207950492E-4	1.0354210929192687E-5	4.141684371677075E-6	0.20724160258997748	8.28336874335415E-6
60-61	0.1332504112899665	1.4081726863702054E-4	1.2425053115031225E-5	4.141684371677075E-6	0.20811756883458715	8.28336874335415E-6
62-63	0.13355275424909896	1.4910063738037468E-4	1.2425053115031225E-5	4.141684371677075E-6	0.20905773118695786	8.28336874335415E-6
64-65	0.13358381688188653	1.553131639378903E-4	1.2425053115031225E-5	4.141684371677075E-6	0.21012007322829304	8.28336874335415E-6
66-67	0.13368114646462093	1.760215857962757E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21085315136207988	1.0354210929192687E-5
68-69	0.1337536259411253	1.8223411235379129E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21171048002701703	1.2425053115031225E-5
70-71	0.13394000173785076	1.8223411235379129E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21256987953414003	1.65667374867083E-5
72-73	0.1339959144768684	1.9465916546882253E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21318906134770577	1.65667374867083E-5
74-75	0.13401869374091263	1.9673000765466108E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21398426474706775	1.65667374867083E-5
76-77	0.13407460647993028	2.0294253421217667E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21486851436042081	1.65667374867083E-5
78-79	0.13415122764080628	2.1122590295553082E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21604682356416294	1.65667374867083E-5
80-81	0.13420299869545227	2.153675873272079E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21696834833686107	1.65667374867083E-5
82-83	0.13422991964386816	2.1950927169888496E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21792921911109014	2.0708421858385375E-5
84-85	0.13428169069851414	2.2986348262807766E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21888594820094756	2.0708421858385375E-5
86-87	0.13435209933283265	2.3193432481391618E-4	1.4495895300869763E-5	1.0354210929192687E-5	0.21988409413452176	2.485010623006245E-5
88-89	0.1343873036499919	2.3193432481391618E-4	1.863757967254684E-5	1.2425053115031225E-5	0.22082011480252076	2.485010623006245E-5
90-91	0.13442043712496532	2.443593779289474E-4	2.0708421858385375E-5	1.2425053115031225E-5	0.22144343830045815	2.485010623006245E-5
92-93	0.13444942891556705	2.485010623006245E-4	2.0708421858385375E-5	1.2425053115031225E-5	0.22200049484844875	2.485010623006245E-5
94-95	0.13449705828584133	2.485010623006245E-4	2.0708421858385375E-5	1.2425053115031225E-5	0.22270665203381967	2.485010623006245E-5
96-97	0.13450327081239885	2.5678443104397864E-4	2.0708421858385375E-5	1.2425053115031225E-5	0.2232616377396244	2.485010623006245E-5
98-99	0.1345219083920714	2.650677997873328E-4	2.277926404422391E-5	1.2425053115031225E-5	0.22391395302816353	2.8991790601739523E-5
100-101	0.1345550418670448	2.6713864197317133E-4	2.485010623006245E-5	1.2425053115031225E-5	0.22449171799801249	3.31334749734166E-5
102-103	0.13456746692015983	2.692094841590099E-4	2.6920948415900986E-5	1.2425053115031225E-5	0.2250570579147464	3.727515934509367E-5
104-105	0.13460060039513327	2.7128032634484843E-4	2.8991790601739523E-5	1.2425053115031225E-5	0.22564517709552456	4.3487685902609285E-5
106-107	0.134629592185735	2.7335116853068693E-4	3.31334749734166E-5	1.2425053115031225E-5	0.22624365048723188	4.97002124601249E-5
108-109	0.13466479650289426	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.22681313208833748	4.97002124601249E-5
110-111	0.13472070924191187	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.2274053929534873	4.97002124601249E-5
112-113	0.1347372759793986	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.22798315792333626	4.97002124601249E-5
114-115	0.13475177187469947	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.22863961489624707	4.97002124601249E-5
116-117	0.13476833861218618	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.22915732544270673	4.97002124601249E-5
118-119	0.13477248029655786	2.899179060173952E-4	3.727515934509367E-5	1.2425053115031225E-5	0.2296729651469805	5.591273901764051E-5
120-121	0.13477869282311536	2.899179060173952E-4	3.727515934509367E-5	1.65667374867083E-5	0.2303232095933338	5.7983581203479045E-5
122-123	0.13479940124497375	2.899179060173952E-4	3.727515934509367E-5	1.65667374867083E-5	0.23079950329607668	6.212526557515612E-5
124-125	0.13483046387776132	2.9198874820323377E-4	3.727515934509367E-5	1.65667374867083E-5	0.23132549721127965	6.833779213267173E-5
126-127	0.13486566819492057	2.9820127476074936E-4	3.727515934509367E-5	1.65667374867083E-5	0.23196124576233207	8.076284524770295E-5
128-129	0.13487395156366394	3.04413801318265E-4	3.727515934509367E-5	1.65667374867083E-5	0.23266326126333137	9.318789836273418E-5
130-131	0.13488016409022147	3.106263278757806E-4	3.934600153093221E-5	2.0708421858385375E-5	0.23333835581591472	9.525874054857272E-5
132-133	0.13490294335426567	3.1269717006161916E-4	4.555852808844782E-5	2.0708421858385375E-5	0.2339285458388787	9.525874054857272E-5
134-135	0.13492158093393825	3.209805388049733E-4	4.555852808844782E-5	2.0708421858385375E-5	0.2344773190181259	9.525874054857272E-5
136-137	0.13492986430268158	3.2305138099081185E-4	4.555852808844782E-5	2.0708421858385375E-5	0.23505094230360318	9.525874054857272E-5
138-139	0.13496092693546916	3.2305138099081185E-4	4.555852808844782E-5	2.0708421858385375E-5	0.2356784074859123	9.94004249202498E-5
>>END_MODULE
