##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24091839
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.869040964452736	37.0	35.0	38.0	33.0	39.0
2	35.48405980963097	35.0	34.0	38.0	33.0	39.0
3	35.60593776174579	36.0	35.0	38.0	33.0	39.0
4	35.67291259915858	36.0	35.0	38.0	33.0	39.0
5	35.597232697761264	36.0	35.0	38.0	33.0	39.0
6	35.538737412283055	36.0	35.0	37.0	33.0	39.0
7	35.56681081921558	35.0	34.0	38.0	33.0	39.0
8	35.62761136665408	36.0	35.0	38.0	33.0	39.0
9	35.64616926918696	36.0	35.0	38.0	33.0	39.0
10-14	35.63986455330371	36.0	35.0	37.8	33.0	39.0
15-19	35.61475603419067	36.0	35.0	38.0	33.0	39.0
20-24	35.60102030401249	36.0	35.0	38.0	33.0	39.0
25-29	35.58519526882111	36.0	35.0	38.0	33.0	39.0
30-34	35.56562387786171	36.0	35.0	38.0	33.0	39.0
35-39	35.539008856899635	36.0	35.0	38.0	33.0	39.0
40-44	35.52122806399295	36.0	35.0	38.0	33.0	39.0
45-49	35.492556620521995	36.0	35.0	38.0	33.0	39.0
50-54	35.47306476686982	36.0	35.0	38.0	33.0	39.0
55-59	35.4478568863091	36.0	34.8	38.0	33.0	39.0
60-64	35.40215040454155	36.0	34.0	38.0	33.0	39.0
65-69	35.37565311639348	36.0	34.0	38.0	32.8	39.0
70-74	35.34724179420259	36.0	34.0	38.0	32.0	39.0
75-79	35.296687230891756	36.0	34.0	38.0	32.0	39.0
80-84	35.25392144617935	36.0	34.0	38.0	32.0	39.0
85-89	35.20313149195459	36.0	34.0	38.0	32.0	39.0
90-94	35.150345251767625	36.0	34.0	38.0	32.0	39.0
95-99	35.08399880141985	36.0	34.0	38.0	32.0	39.0
100-104	35.03485653378307	36.0	34.0	38.0	32.0	39.0
105-109	34.97449674140692	35.0	34.0	37.4	31.4	39.0
110-114	34.894638910711635	35.0	34.0	37.0	31.0	39.0
115-119	34.82975002447924	35.0	34.0	37.0	31.0	39.0
120-124	34.75732748338556	35.0	34.0	37.0	31.0	39.0
125-129	34.67608072592549	35.0	34.0	37.0	30.6	39.0
130-134	34.573884974077735	35.0	34.0	37.0	30.0	39.0
135-139	34.51469797718638	35.0	34.0	37.0	30.0	39.0
140-144	34.39169683974727	35.0	34.0	37.0	30.0	39.0
145-149	34.31365870409478	35.0	34.0	37.0	29.4	39.0
150	34.241886557518505	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	123.0
22	1019.0
23	5506.0
24	19168.0
25	50736.0
26	111581.0
27	211765.0
28	329032.0
29	445063.0
30	583629.0
31	772038.0
32	1049760.0
33	1496456.0
34	2288506.0
35	3999335.0
36	1.0600293E7
37	2127819.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.369663519667384	19.532713961769378	14.542796006564712	31.554826511998524
2	25.163894711399987	25.848952419115868	31.258311165038087	17.728841704446058
3	20.828277160577073	29.21228636801035	28.982328829276998	20.97710764213558
4	24.479746025199653	33.014055091435736	21.77507080302172	20.73112808034289
5	26.398038771552475	34.17634494402856	21.650505799910086	17.775110484508883
6	20.327622146237985	38.425854497865444	21.8019803303517	19.444543025544874
7	21.55580568174974	20.97071958682772	36.22754161689359	21.24593311452895
8	22.6145726315611	23.586472591425427	27.737065388398026	26.06188938861545
9	24.21640788816495	22.878892723797463	27.89861330220578	25.006086085831804
10-14	23.872871639230198	28.562938678114197	24.909440080518554	22.654749602137056
15-19	23.839470287012958	27.61080131740877	26.36671032045333	22.18301807512494
20-24	23.710126072152484	27.67465862610156	26.325894839327123	22.28932046241883
25-29	23.81928004748828	27.375702618633635	26.30902522634325	22.49599210753484
30-34	23.561686594369156	27.207061279132738	26.56132394044307	22.66992818605504
35-39	23.844020375530487	27.053728858141547	26.584706962386722	22.517543803941244
40-44	23.953187633372448	26.85946722456513	26.652739128797933	22.53460601326449
45-49	23.873121516377395	26.768376627454632	26.750572258099513	22.60792959806846
50-54	23.996129145641394	26.71446957619134	26.553520468072193	22.73588081009507
55-59	24.065931205998844	26.724107694726	26.597889849753685	22.612071249521467
60-64	23.771116019827296	26.769067317775118	26.684950036400295	22.77486662599729
65-69	23.953533808689322	26.771551976584266	26.70692760316056	22.56798661156585
70-74	24.0839605478021	26.739775240902112	26.44678224854483	22.729481962750956
75-79	23.857280467464523	26.819540011038594	26.73779448717053	22.585385034326354
80-84	24.120183802657685	26.826683731587973	26.470601322469417	22.58253114328492
85-89	23.869871453150587	26.834333402277842	26.779191077941373	22.516604066630197
90-94	23.894626724012227	26.621181554467473	26.795399056087	22.6887926654333
95-99	23.791449046293227	26.861509409887724	26.841882846718345	22.505158697100708
100-104	24.04637188551692	26.692297752778444	26.781205037938367	22.480125323766277
105-109	23.826405281888192	26.766585149435873	26.90140258699222	22.505606981683716
110-114	23.865380648075213	26.875579258948097	26.797733067081175	22.46130702589552
115-119	23.973575450176302	26.879394304436456	26.93758745440728	22.209442790979967
120-124	23.89264597028064	26.958559701482315	27.097918925989834	22.050875402247208
125-129	23.84038488320592	27.047234386230983	27.016595794532645	22.09578493603045
130-134	23.982938040661168	26.968631426213857	27.086723805640005	21.96170672748497
135-139	23.7492862209481	27.041424276494624	27.31052868151742	21.898760821039858
140-144	23.73953686142432	27.170102705733672	27.132196923613844	21.958163509228164
145-149	23.911898132807547	27.82001158151522	26.534444298751954	21.733645986925282
150	23.531781861899375	27.914635325265124	26.528622410269303	22.024960402566197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	0.5
7	0.5
8	0.0
9	1.0
10	1.5
11	2.5
12	6.0
13	11.5
14	16.5
15	29.0
16	48.5
17	81.5
18	159.5
19	294.5
20	484.0
21	717.0
22	1150.5
23	1917.0
24	3029.0
25	4926.5
26	7601.5
27	11585.5
28	17517.0
29	25236.5
30	35643.5
31	50664.0
32	71922.0
33	98471.5
34	131418.5
35	181300.0
36	254809.0
37	347851.5
38	462172.0
39	602595.0
40	772187.0
41	964005.0
42	1163914.0
43	1361358.5
44	1528406.0
45	1631233.5
46	1668032.5
47	1652589.5
48	1610333.5
49	1560041.0
50	1447346.5
51	1274540.0
52	1066213.0
53	867832.5
54	719925.0
55	593233.0
56	480143.0
57	386573.0
58	315258.5
59	249369.5
60	180224.0
61	116044.5
62	69378.5
63	41321.0
64	25772.0
65	14817.0
66	8643.0
67	4608.5
68	2448.0
69	1435.0
70	846.0
71	646.0
72	466.0
73	364.0
74	279.5
75	182.5
76	114.0
77	37.5
78	8.5
79	2.0
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.1181188368393131
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	8.301566351991643E-7
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.01908447088659359
115-119	0.0
120-124	0.0
125-129	2.490469905597493E-6
130-134	0.0471902539278965
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4091839E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.008054128195404
#Duplication Level	Percentage of total
1	27.444709739427488
2	8.529794692874102
3	5.259319057137887
4	3.6646864188699717
5	2.8096061768208265
6	2.2829213951834295
7	1.8091749311012069
8	1.555080200825286
9	1.3485869748148815
>10	17.491838569899247
>50	6.246455233959719
>100	12.752394533755268
>500	3.5982863026565717
>1k	4.482795488827454
>5k	0.49746457353952966
>10k+	0.22688571030706706
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.656501232637326E-4	4.150783175995822E-6	0.0	0.0	0.0011705208556308216	0.0
2	0.001232782603270759	4.150783175995822E-6	0.0	0.0	0.005678271384762284	0.0
3	0.0018678524291981197	4.150783175995822E-6	0.0	0.0	0.007923845082976024	0.0
4	0.004503599745955466	4.150783175995822E-6	0.0	0.0	0.011070138730380857	0.0
5	0.004644726373939324	4.150783175995822E-6	0.0	0.0	0.012738753567131177	0.0
6	0.005562049455834401	4.150783175995822E-6	0.0	0.0	0.016644640535743244	0.0
7	0.007168402544944784	8.301566351991643E-6	0.0	0.0	0.02041355165954745	0.0
8	0.012240659586011678	1.2452349527987464E-5	0.0	0.0	0.027652517518484163	0.0
9	0.012701396518547214	1.2452349527987464E-5	0.0	0.0	0.03276628239131102	0.0
10-11	0.01325137528936666	1.4527741115985375E-5	0.0	0.0	0.05490863524366073	0.0
12-13	0.013753620053662156	1.6603132703983287E-5	0.0	2.075391587997911E-6	0.06555746948167801	0.0
14-15	0.015716940495908178	2.075391587997911E-5	0.0	4.150783175995822E-6	0.07194344939794758	0.0
16-17	0.018122319346397756	2.490469905597493E-5	0.0	4.150783175995822E-6	0.07598631221136751	0.0
18-19	0.022513847946601336	2.698009064397284E-5	4.150783175995822E-6	4.150783175995822E-6	0.08068084798341879	0.0
20-21	0.026479921271265347	2.905548223197075E-5	4.150783175995822E-6	4.150783175995822E-6	0.08383336780558762	0.0
22-23	0.02818174237342363	4.565861493595404E-5	4.150783175995822E-6	4.150783175995822E-6	0.08727644245007615	0.0
24-25	0.03164764632538014	4.980939811194986E-5	4.150783175995822E-6	4.150783175995822E-6	0.09071121552821268	0.0
26-27	0.03464036099527313	6.018635605193941E-5	4.150783175995822E-6	4.150783175995822E-6	0.0936665731495217	0.0
28-29	0.04134595121609438	6.226174763993732E-5	4.150783175995822E-6	4.150783175995822E-6	0.09605327347571932	0.0
30-31	0.05579275206014783	6.848792240393106E-5	4.150783175995822E-6	4.150783175995822E-6	0.0985416679897288	0.0
32-33	0.06817453827414337	8.301566351991644E-5	4.150783175995822E-6	4.150783175995822E-6	0.10049253608244685	0.0
34-35	0.07796416039472952	8.301566351991644E-5	4.150783175995822E-6	4.150783175995822E-6	0.10215700013602116	4.150783175995822E-6
36-37	0.08012049225465935	8.301566351991644E-5	4.150783175995822E-6	4.150783175995822E-6	0.10360147268126771	4.150783175995822E-6
38-39	0.0821481498361333	8.301566351991644E-5	4.150783175995822E-6	4.150783175995822E-6	0.10492972329758637	4.150783175995822E-6
40-41	0.08316094093107629	8.509105510791436E-5	4.150783175995822E-6	4.150783175995822E-6	0.10616665668403313	4.150783175995822E-6
42-43	0.08337678165622807	8.924183828391016E-5	4.150783175995822E-6	4.150783175995822E-6	0.10754471669846374	4.150783175995822E-6
44-45	0.08397864521674746	9.3392621459906E-5	4.150783175995822E-6	4.150783175995822E-6	0.10866542815598261	4.150783175995822E-6
46-47	0.08481710341829862	1.1207114575188718E-4	4.150783175995822E-6	4.150783175995822E-6	0.10984632596955343	4.150783175995822E-6
48-49	0.08569291866843373	1.1207114575188718E-4	4.150783175995822E-6	4.150783175995822E-6	0.11081760923273645	4.150783175995822E-6
50-51	0.08650232138775292	1.1207114575188718E-4	4.150783175995822E-6	4.150783175995822E-6	0.11188020972579138	4.150783175995822E-6
52-53	0.08725361314260816	1.1829732051588091E-4	4.150783175995822E-6	4.150783175995822E-6	0.11284111603103442	8.301566351991643E-6
54-55	0.08762303284527179	1.349004532198642E-4	4.150783175995822E-6	4.150783175995822E-6	0.1137397605886375	1.4527741115985375E-5
56-57	0.08812942839274329	1.4320201957185584E-4	4.150783175995822E-6	4.150783175995822E-6	0.11465708367053258	1.6603132703983287E-5
58-59	0.08825187649643516	1.4527741115985375E-4	4.150783175995822E-6	4.150783175995822E-6	0.11548724030573174	2.075391587997911E-5
60-61	0.08845318948047096	1.515035859238475E-4	4.150783175995822E-6	4.150783175995822E-6	0.11628626606711094	2.075391587997911E-5
62-63	0.08863997472339077	1.5772976068784123E-4	4.150783175995822E-6	4.150783175995822E-6	0.11715793053407006	2.075391587997911E-5
64-65	0.08865865324768275	1.5980515227583913E-4	6.226174763993732E-6	4.150783175995822E-6	0.11793412698798128	2.075391587997911E-5
66-67	0.08869601029626671	1.6603132703983287E-4	8.301566351991643E-6	4.150783175995822E-6	0.11869994648395252	2.075391587997911E-5
68-69	0.08872714117008668	1.6810671862783078E-4	8.301566351991643E-6	4.150783175995822E-6	0.11947614293786374	2.075391587997911E-5
70-71	0.08883921231583858	1.7018211021582868E-4	8.301566351991643E-6	4.150783175995822E-6	0.12032705348894288	2.490469905597493E-5
72-73	0.08887241858124653	1.7225750180382661E-4	8.301566351991643E-6	4.150783175995822E-6	0.12097872644757421	3.7357048583962396E-5
74-75	0.08889109710553852	1.7433289339182452E-4	8.301566351991643E-6	4.150783175995822E-6	0.12162002244826557	3.943244017196031E-5
76-77	0.08893883111206247	1.7433289339182452E-4	8.301566351991643E-6	4.150783175995822E-6	0.12248961152363669	4.150783175995822E-5
78-79	0.08899279129335042	1.9923759244779943E-4	8.301566351991643E-6	4.150783175995822E-6	0.12376597735025541	4.565861493595404E-5
80-81	0.08902807295034637	2.137653335637848E-4	8.301566351991643E-6	4.150783175995822E-6	0.12458160624433859	4.565861493595404E-5
82-83	0.08905505304099035	2.1584072515178272E-4	1.0376957939989553E-5	4.150783175995822E-6	0.12531214408331387	4.980939811194986E-5
84-85	0.08908410852322232	2.1584072515178272E-4	1.2452349527987464E-5	4.150783175995822E-6	0.1263394629193728	4.980939811194986E-5
86-87	0.08911731478863029	2.1791611673978062E-4	1.2452349527987464E-5	4.150783175995822E-6	0.127138488680752	4.980939811194986E-5
88-89	0.08913806870451027	2.2206689991577646E-4	1.2452349527987464E-5	4.150783175995822E-6	0.12802883167200313	4.980939811194986E-5
90-91	0.08915467183721426	2.345192494437639E-4	1.2452349527987464E-5	4.150783175995822E-6	0.12871578628763042	4.980939811194986E-5
92-93	0.08917127496991824	2.3659464103176184E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.12937783620420176	5.81109644639415E-5
94-95	0.08923768750073416	2.3867003261975975E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13040307964867273	5.81109644639415E-5
96-97	0.08925844141661415	2.4074542420775765E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13115229601193998	5.81109644639415E-5
98-99	0.08927504454931813	2.448962073837535E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13183717523597926	5.81109644639415E-5
100-101	0.0893082508147261	2.448962073837535E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.1325386575927226	6.226174763993732E-5
102-103	0.08932485394743009	2.490469905597493E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13323806455787787	7.056331399192897E-5
104-105	0.08933730629695807	2.511223821477472E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13394369769779718	7.263870557992687E-5
106-107	0.08935806021283804	2.531977737357451E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.1346098983975445	7.471409716792479E-5
108-109	0.08939126647824602	2.5734855691174097E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13536741632716373	7.471409716792479E-5
110-111	0.08943900048476996	2.594239484997389E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13613531121472294	7.471409716792479E-5
112-113	0.08945560361747396	2.677255148517305E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.1369820709826261	7.678948875592271E-5
114-115	0.08947635753335392	2.739516896157242E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13773958891224536	7.886488034392061E-5
116-117	0.08948465909970593	2.7810247279172007E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13841824196152067	7.886488034392061E-5
118-119	0.0895054130155859	2.905548223197075E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13915708136684793	7.886488034392061E-5
120-121	0.08951163919034989	2.9470560549570336E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.14001006730951504	7.886488034392061E-5
122-123	0.08952201614828989	2.9470560549570336E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.14073437897372632	8.924183828391016E-5
124-125	0.08953031771464187	2.9470560549570336E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.14152095238557755	9.961879622389971E-5
126-127	0.08953654388940588	3.0093178025969707E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.14223073630867283	1.1207114575188718E-4
128-129	0.08954899623893386	3.071579550236908E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.1431397578242159	1.16221928927883E-4
130-131	0.08955729780528585	3.092333466116887E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.14411519187057492	1.16221928927883E-4
132-133	0.08957597632957784	3.1130873819968665E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.1449308207646581	1.16221928927883E-4
134-135	0.08958842867910582	3.1130873819968665E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.14568211251951335	1.2037271210387883E-4
136-137	0.08960295642022181	3.1338412978768455E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.14648321367248054	1.2452349527987465E-4
138-139	0.08964031346880577	3.1753491296368036E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.14739638597119964	1.2452349527987465E-4
>>END_MODULE
