##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.S.3.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24166152
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.92022486658199	36.0	34.0	38.0	30.0	39.0
2	34.77115194011856	35.0	34.0	38.0	31.0	39.0
3	34.959282181126724	35.0	34.0	38.0	31.0	39.0
4	34.91311541862353	36.0	34.0	38.0	31.0	39.0
5	34.777896828589014	35.0	34.0	37.0	31.0	39.0
6	34.76667158263343	35.0	34.0	37.0	31.0	39.0
7	34.76699757578285	35.0	34.0	37.0	32.0	39.0
8	34.92836281920266	35.0	34.0	37.0	32.0	39.0
9	34.91321928290445	35.0	34.0	37.0	32.0	39.0
10-14	34.88958001257296	35.0	34.0	37.4	31.4	39.0
15-19	34.88627198074398	35.0	34.0	37.0	31.0	39.0
20-24	34.895535855273934	35.0	34.0	37.0	31.0	39.0
25-29	34.88605277331699	35.0	34.0	37.0	31.0	39.0
30-34	34.86886481554863	35.0	34.0	37.0	31.0	39.0
35-39	34.86484621962156	35.0	34.0	37.0	31.0	39.0
40-44	34.800430345716606	35.0	34.0	37.0	31.0	39.0
45-49	34.69890904435261	35.0	34.0	37.0	31.0	39.0
50-54	34.66548847329935	35.0	34.0	37.0	31.0	39.0
55-59	34.65493424853075	35.0	34.0	37.0	31.0	39.0
60-64	34.62620662983498	35.0	34.0	37.0	31.0	39.0
65-69	34.60736453201155	35.0	34.0	37.0	30.6	39.0
70-74	34.57962938410716	35.0	34.0	37.0	30.0	39.0
75-79	34.55871793738614	35.0	34.0	37.0	30.0	39.0
80-84	34.530140545337964	35.0	34.0	37.0	30.0	39.0
85-89	34.51212705274717	35.0	34.0	37.0	30.0	39.0
90-94	34.48495511407857	35.0	34.0	37.0	30.0	39.0
95-99	34.449004541558786	35.0	34.0	37.0	30.0	39.0
100-104	34.412310838730136	35.0	34.0	37.0	30.0	39.0
105-109	34.353550585960065	35.0	34.0	37.0	30.0	39.0
110-114	34.32205716491397	35.0	34.0	37.0	30.0	39.0
115-119	34.27136254874173	35.0	34.0	37.0	30.0	39.0
120-124	34.23593902744632	35.0	34.0	37.0	29.6	39.0
125-129	34.179199220463396	35.0	34.0	37.0	29.0	39.0
130-134	34.13091344455667	35.0	34.0	37.0	29.0	39.0
135-139	34.07919042303466	35.0	34.0	37.0	29.0	39.0
140-144	34.05278100543272	35.0	34.0	37.0	29.0	39.0
145-149	33.984038087652515	35.0	34.0	37.0	29.0	39.0
150	33.955009759104385	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	22.0
22	469.0
23	4396.0
24	26163.0
25	95749.0
26	227510.0
27	395978.0
28	569137.0
29	736467.0
30	914493.0
31	1144069.0
32	1459763.0
33	1916373.0
34	2647684.0
35	4124441.0
36	8508229.0
37	1395207.0
38	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.96439186821282	10.246348462448543	14.648900256901696	39.14035941243694
2	23.085510439374957	12.041851019250021	36.693791162513506	28.178847378861516
3	21.52396025031564	16.95906282714952	28.424324806012578	33.09265211652226
4	27.072419650827268	24.73357359228289	21.80846529616553	26.385541460724315
5	26.926658539593003	30.466110847657095	23.843869976752597	18.76336063599731
6	19.781886493796918	36.346096415177385	25.105833026740353	18.766184064285337
7	16.557365442346466	24.31763995260963	40.34949218721389	18.77550241783001
8	19.145690375866092	23.59207408152558	31.25352326745864	26.00871227514969
9	20.084774013483	22.168806327797927	32.64778989041358	25.098629768305496
10-14	20.95773062335805	28.62557640937447	26.710311967154205	23.706381000113275
15-19	21.213312537378275	27.44833262323421	27.3607430187212	23.97761182066631
20-24	21.149459051736518	27.540782427999982	27.26540432301442	24.044354197249085
25-29	21.120320663849196	27.74121769786716	26.926870035797467	24.211591602486177
30-34	21.123748527981203	27.5053070720322	27.150993841263126	24.21995055872347
35-39	21.423040924907777	27.264491725857535	27.053725948526484	24.2587414007082
40-44	21.57855177882764	27.383461994647373	26.868559351307496	24.169426875217486
45-49	21.81416442835105	27.319358192884003	26.778095563890265	24.088381814874683
50-54	21.803950433653025	27.534922643919757	26.698214096886613	23.962912825540602
55-59	21.71689497883172	27.493984415799723	26.763942522177754	24.025178083190806
60-64	21.92180430988988	27.318455297254246	26.567078278525212	24.192662114330663
65-69	21.764760762404737	27.422162547374995	26.756740385804196	24.056336304416075
70-74	22.133177714576576	27.182754834017842	26.6844577838466	23.99960966755898
75-79	21.999082052411502	27.067025699851033	26.677684200539286	24.25620804719818
80-84	22.235933720685992	27.079550139923324	26.69510651536045	23.989409624030234
85-89	22.10128672561516	27.065885643213218	26.711757712849366	24.121069918322256
90-94	22.098739018150813	26.833140545026264	26.69887619567703	24.369244241145896
95-99	22.30934030120536	26.849034480449312	26.877040680810204	23.96458453753512
100-104	22.255992116671123	26.704142625831196	26.92301179670568	24.116853460792004
105-109	22.343574036683897	26.65829349823002	26.82451325061767	24.17361921446841
110-114	22.326169961598183	26.72294043548597	26.794434811619517	24.156454791296333
115-119	22.42659242886884	26.88880862582555	26.708799367894443	23.975799577411163
120-124	22.376286083764374	26.749677451017433	26.70445955753633	24.169576907681865
125-129	22.75176494639412	26.72018165070164	26.585112368324104	23.942941034580137
130-134	22.505375005857374	26.98838222772208	26.46701414938336	24.039228617037185
135-139	22.583906085751412	26.809710105807	26.53778967587076	24.068594132570826
140-144	22.56739872490856	26.643287645384383	26.79116663790738	23.998146991799672
145-149	22.37938634640938	27.096552331200837	26.72703723287915	23.797024089510632
150	22.12566455181699	26.638469866402513	26.86700902781206	24.36885655396843
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.5
10	2.0
11	4.5
12	9.0
13	15.0
14	19.0
15	41.0
16	91.0
17	193.0
18	451.0
19	825.5
20	1344.0
21	2164.0
22	3619.0
23	6035.5
24	9871.5
25	15361.5
26	22131.5
27	32165.0
28	49862.5
29	74472.5
30	102139.5
31	129966.0
32	157933.0
33	190910.0
34	227332.0
35	275050.5
36	344158.5
37	418539.5
38	493923.5
39	603584.5
40	752507.0
41	915034.0
42	1083559.5
43	1249744.0
44	1396244.0
45	1505990.5
46	1577120.5
47	1591655.5
48	1541077.5
49	1484418.0
50	1392560.0
51	1233384.5
52	1040539.0
53	869575.0
54	725293.5
55	596872.0
56	499324.5
57	404828.0
58	330841.0
59	268535.5
60	201780.0
61	138498.0
62	82329.5
63	48262.0
64	31083.5
65	18978.0
66	12174.0
67	6000.5
68	2418.5
69	1122.5
70	860.0
71	606.5
72	331.0
73	144.0
74	90.5
75	62.0
76	35.5
77	30.5
78	15.5
79	3.5
80	2.5
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.0966389684216173E-4
2	2.069009580010918E-4
3	9.517444068050222E-5
4	1.6552076640087343E-5
5	4.965622992026203E-5
6	7.862236404041487E-5
7	2.5241916876133193E-4
8	7.862236404041487E-5
9	8.276038320043671E-5
10-14	1.5476191658481665E-4
15-19	1.7462440855292146E-4
20-24	1.3738223611272493E-4
25-29	3.5255923243386036E-4
30-34	7.870512442361532E-4
35-39	7.423606373079172E-4
40-44	9.285714995088998E-4
45-49	8.789152695886378E-4
50-54	5.238732256587644E-4
55-59	3.591800630898953E-4
60-64	2.822129067134892E-4
65-69	3.302139289697425E-4
70-74	3.5090402476985167E-4
75-79	2.7476447222544987E-4
80-84	2.482811496013101E-4
85-89	3.4345559028181236E-4
90-94	3.9062900870606124E-4
95-99	3.095238331696333E-4
100-104	4.849758455545591E-4
105-109	2.937993603615503E-4
110-114	2.292462614652097E-4
115-119	2.5490198025734505E-4
120-124	2.2759105380120096E-4
125-129	1.4731348209677734E-4
130-134	1.605551434088472E-4
135-139	1.5889993574483846E-4
140-144	1.737968047209171E-4
145-149	2.0193533500906558E-4
150	2.193150154811573E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4166152E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.77056599598994
#Duplication Level	Percentage of total
1	33.05300073785521
2	9.083065436175982
3	5.204976752461145
4	3.690965977175366
5	2.7139223605285494
6	2.1927547213466214
7	1.8169956744992646
8	1.483463790667232
9	1.273527112223424
>10	16.509087581882856
>50	5.503670395400556
>100	10.264701271953227
>500	3.3565938604392485
>1k	3.3541818185825543
>5k	0.3873718262100715
>10k+	0.11172068259879203
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.276038320043671E-6	0.0	0.0	8.276038320043671E-6	2.606952070813756E-4	2.4828114960131014E-5
2	1.6552076640087343E-5	4.551821076024019E-5	0.0	2.069009580010918E-5	0.002337980825412337	3.3104153280174686E-5
3	2.069009580010918E-5	5.79322682403057E-5	0.0	2.069009580010918E-5	0.004564235133504084	4.965622992026203E-5
4	4.965622992026203E-5	6.620830656034937E-5	0.0	2.069009580010918E-5	0.006645658770995068	6.207028740032753E-5
5	5.79322682403057E-5	6.620830656034937E-5	0.0	2.4828114960131014E-5	0.007895340557321662	6.620830656034937E-5
6	7.448434488039304E-5	6.620830656034937E-5	0.0	2.896613412015285E-5	0.009546410202170374	6.620830656034937E-5
7	9.517444068050222E-5	9.517444068050222E-5	0.0	2.896613412015285E-5	0.010998854927338039	6.620830656034937E-5
8	1.8207284304096077E-4	9.517444068050222E-5	0.0	2.896613412015285E-5	0.014300994217035463	7.03463257203712E-5
9	2.606952070813756E-4	9.931245984052406E-5	0.0	2.896613412015285E-5	0.01715622743745053	7.03463257203712E-5
10-11	3.227654944817032E-4	1.0345047900054589E-4	0.0	3.1035143700163765E-5	0.03016202165739916	1.0345047900054589E-4
12-13	3.620766765019106E-4	1.0345047900054589E-4	0.0	3.3104153280174686E-5	0.03896565742034561	1.386236418607315E-4
14-15	3.9311182020207436E-4	1.0965750774057865E-4	0.0	3.3104153280174686E-5	0.04546855453031993	1.4896868976078609E-4
16-17	4.2207795432222723E-4	1.2000255564063323E-4	0.0	3.3104153280174686E-5	0.0494989851921812	1.4896868976078609E-4
18-19	4.8414824172255473E-4	1.282785939606769E-4	0.0	3.3104153280174686E-5	0.052881815855499045	1.613827472408516E-4
20-21	7.634645350240287E-4	1.531067089208079E-4	0.0	3.517316286018561E-5	0.0554701468400927	1.6552076640087343E-4
22-23	0.0010200217229453825	1.5724472808082974E-4	0.0	3.724217244019652E-5	0.05835641520420794	1.6758977598088434E-4
24-25	0.0011027821061458192	1.634517568208625E-4	0.0	4.551821076024019E-5	0.06102543756242202	1.6965878556089524E-4
26-27	0.0011420932881660265	1.6552076640087343E-4	0.0	4.758722034025111E-5	0.06346893787641492	1.8207284304096075E-4
28-29	0.0011917495180862885	1.6552076640087343E-4	0.0	6.620830656034937E-5	0.06610278707176881	1.9034888136100443E-4
30-31	0.001309683064146911	1.6552076640087343E-4	0.0	9.103642152048039E-5	0.06916492125018497	2.0483194842108087E-4
32-33	0.0013510632557471292	1.6965878556089524E-4	0.0	9.103642152048039E-5	0.07200980942269998	2.8345431246149574E-4
34-35	0.0014420996772676097	1.7172779514090618E-4	0.0	9.103642152048039E-5	0.07461676149351373	2.9793737952157217E-4
36-37	0.0014752038305477842	1.75865814300928E-4	0.0	9.310543110049131E-5	0.07702508864464644	3.02075398681594E-4
38-39	0.0014979629359279044	1.8207284304096077E-4	0.0	9.931245984052406E-5	0.07994239215246185	3.0621341784161586E-4
40-41	0.0015289980796280682	1.8414185262097168E-4	0.0	9.931245984052406E-5	0.08230106307367428	3.124204465816486E-4
42-43	0.0015310670892080792	1.882798717809935E-4	0.0	9.931245984052406E-5	0.08460180172664641	3.144894561616595E-4
44-45	0.0015476191658481664	1.9034888136100443E-4	0.0	1.0138146942053497E-4	0.08722323686452027	3.144894561616595E-4
46-47	0.0015703782712282867	1.9034888136100443E-4	0.0	1.0345047900054589E-4	0.09165919340406367	3.144894561616595E-4
48-49	0.0015910683670283957	1.9448690052102627E-4	0.0	1.0345047900054589E-4	0.09435304387723789	3.227654944817032E-4
50-51	0.001609689453248494	2.1517699632113546E-4	0.0	1.0345047900054589E-4	0.09711517166655245	3.227654944817032E-4
52-53	0.0016303795490486032	2.1517699632113546E-4	0.0	1.0345047900054589E-4	0.09948625664524496	3.3104153280174686E-4
54-55	0.0016676217214887997	2.1517699632113546E-4	0.0	1.0345047900054589E-4	0.10131939913313465	3.351795519617687E-4
56-57	0.0017255539897291054	2.213840250611682E-4	0.0	1.0345047900054589E-4	0.10326840615750493	3.372485615417796E-4
58-59	0.0017979693250294874	2.3793610170125555E-4	0.0	1.1379552690060048E-4	0.10532293267045577	3.4345559028181236E-4
60-61	0.0018807297082299242	2.4000511128126646E-4	0.0	1.2000255564063323E-4	0.10732987196306636	3.4345559028181236E-4
62-63	0.0019324549477301972	2.4621214002129924E-4	0.0	1.2000255564063323E-4	0.10971130198965892	3.4345559028181236E-4
64-65	0.001955214053110317	2.482811496013101E-4	0.0	1.282785939606769E-4	0.11227066684013243	3.4345559028181236E-4
66-67	0.0019738351393304154	2.648332262413975E-4	0.0	1.3241661312069874E-4	0.11460657865596476	3.4345559028181236E-4
68-69	0.0019821111776504594	2.7517827414145205E-4	0.0	1.3241661312069874E-4	0.11653489558453492	3.5173162860185604E-4
70-71	0.0020131463213506227	2.77247283721463E-4	0.0	1.3241661312069874E-4	0.1185956291262258	3.5173162860185604E-4
72-73	0.002017284340510645	2.896613412015285E-4	0.0	1.3241661312069874E-4	0.12053015308353601	3.5173162860185604E-4
74-75	0.002025560378830688	2.896613412015285E-4	0.0	1.406926514407424E-4	0.12216053263258461	3.53800638181867E-4
76-77	0.0020503884937908196	2.896613412015285E-4	0.0	1.406926514407424E-4	0.12348883678295164	3.5586964776187786E-4
78-79	0.0020814236374909834	2.896613412015285E-4	0.0	1.5724472808082974E-4	0.12472610451179816	3.5586964776187786E-4
80-81	0.002116596800351169	2.9173035078153937E-4	4.138019160021836E-6	1.613827472408516E-4	0.12604613262384512	3.5586964776187786E-4
82-83	0.0021372868961512777	2.937993603615503E-4	4.138019160021836E-6	1.6552076640087343E-4	0.12760823485675338	3.5586964776187786E-4
84-85	0.0021765980781714854	2.937993603615503E-4	4.138019160021836E-6	1.6965878556089524E-4	0.12921585530042184	3.5793865734188874E-4
86-87	0.002215909260191693	2.9793737952157217E-4	4.138019160021836E-6	1.6965878556089524E-4	0.13064347191062936	3.600076669218997E-4
88-89	0.002238668365571813	2.9793737952157217E-4	4.138019160021836E-6	1.75865814300928E-4	0.13203798436755676	3.620766765019106E-4
90-91	0.002251082423051879	3.0621341784161586E-4	4.138019160021836E-6	1.7793482388093893E-4	0.13406768276554745	3.6414568608192154E-4
92-93	0.0022717725188519874	3.144894561616595E-4	4.138019160021836E-6	1.7793482388093893E-4	0.13593186039713728	3.6414568608192154E-4
94-95	0.0023255667679322714	3.2069648490169224E-4	4.138019160021836E-6	1.7793482388093893E-4	0.13751672173542565	3.662146956619325E-4
96-97	0.0023876370553325994	3.289725232217359E-4	4.138019160021836E-6	1.7793482388093893E-4	0.13883054281873258	3.7035271482195424E-4
98-99	0.002464190409793003	3.3311054238175774E-4	4.138019160021836E-6	1.7793482388093893E-4	0.13984642652251794	3.724217244019652E-4
100-101	0.002567640888793549	3.351795519617687E-4	4.138019160021836E-6	1.8000383346094986E-4	0.14098645080110395	3.724217244019652E-4
102-103	0.002590399994173669	3.393175711217905E-4	4.138019160021836E-6	1.8207284304096077E-4	0.1420726808306097	3.78628753141998E-4
104-105	0.0026152281091338	3.4345559028181236E-4	4.138019160021836E-6	1.9034888136100443E-4	0.14316718689843547	3.8276677230201974E-4
106-107	0.002644194243253953	3.4552459986182324E-4	4.138019160021836E-6	1.9655591010103718E-4	0.1442968661291214	3.848357818820307E-4
108-109	0.0026710913677940947	3.51731628601856E-4	4.138019160021836E-6	2.0276293884106993E-4	0.14543689040770744	3.848357818820307E-4
110-111	0.002712471559394313	3.620766765019106E-4	4.138019160021836E-6	2.0276293884106993E-4	0.14646311915939286	3.869047914620416E-4
112-113	0.002786955904274706	3.6414568608192154E-4	4.138019160021836E-6	2.0690095800109177E-4	0.1476114194762989	3.8897380104205255E-4
114-115	0.0028241980767149026	3.6828370524194336E-4	4.138019160021836E-6	2.0690095800109177E-4	0.14884248017640542	3.9311182020207436E-4
116-117	0.0028841993545352194	3.76559743561987E-4	4.138019160021836E-6	2.1103897716111362E-4	0.1497693964682503	3.9311182020207436E-4
118-119	0.0029317865748754706	3.8483578188203073E-4	4.138019160021836E-6	2.2138402506116818E-4	0.15066941563555503	3.9311182020207436E-4
120-121	0.003064203187996169	3.8897380104205255E-4	4.138019160021836E-6	2.317290729612228E-4	0.15165219518606024	3.9311182020207436E-4
122-123	0.003302139289697425	3.9311182020207436E-4	4.138019160021836E-6	2.3586709212124462E-4	0.15252117920966482	3.9311182020207436E-4
124-125	0.003430417883658102	3.9311182020207436E-4	4.138019160021836E-6	2.441431304412883E-4	0.15336326610872925	3.9311182020207436E-4
126-127	0.003473867084838331	3.951808297820853E-4	4.138019160021836E-6	2.482811496013101E-4	0.1541908699407336	3.9311182020207436E-4
128-129	0.0035297303434986254	3.993188489421071E-4	4.138019160021836E-6	2.565571879213538E-4	0.15491088527457741	3.951808297820853E-4
130-131	0.003664215966199335	4.0138785852211805E-4	4.138019160021836E-6	2.6690223582140837E-4	0.15561848655094118	3.9724983936209623E-4
132-133	0.0037759424835199246	4.0552587768213986E-4	4.138019160021836E-6	2.731092645614411E-4	0.15630125971234476	3.9724983936209623E-4
134-135	0.003852495837980329	4.0552587768213986E-4	4.138019160021836E-6	2.731092645614411E-4	0.15712886354434913	3.9724983936209623E-4
136-137	0.003955946316980875	4.2207795432222723E-4	4.138019160021836E-6	2.7517827414145205E-4	0.15792129421349332	3.993188489421071E-4
138-139	0.0040159475948011916	4.2621597348224905E-4	4.138019160021836E-6	2.77247283721463E-4	0.158616481432377	4.0138785852211805E-4
>>END_MODULE
