##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.S.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24166152
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.76898328703717	37.0	35.0	38.0	33.0	39.0
2	35.35263951000557	35.0	34.0	37.0	33.0	39.0
3	35.48712144986922	35.0	34.0	37.0	33.0	39.0
4	35.56391104384347	36.0	35.0	38.0	33.0	39.0
5	35.48431703152409	36.0	35.0	38.0	33.0	39.0
6	35.43563795344828	35.0	35.0	37.0	33.0	39.0
7	35.46556299074838	35.0	34.0	38.0	33.0	39.0
8	35.57023815789953	36.0	35.0	38.0	33.0	39.0
9	35.55351054648667	36.0	35.0	38.0	33.0	39.0
10-14	35.52809331829081	35.8	35.0	37.8	33.0	39.0
15-19	35.50661332428928	36.0	35.0	38.0	33.0	39.0
20-24	35.4948848869278	36.0	35.0	38.0	33.0	39.0
25-29	35.47736337171098	36.0	34.8	38.0	33.0	39.0
30-34	35.46243817385573	36.0	34.4	38.0	33.0	39.0
35-39	35.43335175579464	36.0	34.0	38.0	33.0	39.0
40-44	35.41795527893725	36.0	34.0	38.0	33.0	39.0
45-49	35.39089678820194	36.0	34.0	38.0	32.8	39.0
50-54	35.36975974495236	36.0	34.0	38.0	32.4	39.0
55-59	35.34650459866346	36.0	34.0	38.0	32.0	39.0
60-64	35.30507150662629	36.0	34.0	38.0	32.0	39.0
65-69	35.27791120406758	36.0	34.0	38.0	32.0	39.0
70-74	35.245750477775694	36.0	34.0	38.0	32.0	39.0
75-79	35.19765400796949	36.0	34.0	38.0	32.0	39.0
80-84	35.15775967973718	36.0	34.0	38.0	32.0	39.0
85-89	35.109372216147605	36.0	34.0	38.0	32.0	39.0
90-94	35.05782351282075	35.8	34.0	38.0	32.0	39.0
95-99	34.992565245803306	35.2	34.0	38.0	31.0	39.0
100-104	34.944577854182164	35.0	34.0	38.0	31.0	39.0
105-109	34.88667645556479	35.0	34.0	37.2	31.0	39.0
110-114	34.81199939485608	35.0	34.0	37.0	31.0	39.0
115-119	34.73725598514815	35.0	34.0	37.0	31.0	39.0
120-124	34.66433239350642	35.0	34.0	37.0	30.2	39.0
125-129	34.584941367578914	35.0	34.0	37.0	30.0	39.0
130-134	34.497133519643505	35.0	34.0	37.0	30.0	39.0
135-139	34.42348027935933	35.0	34.0	37.0	30.0	39.0
140-144	34.29578225776284	35.0	34.0	37.0	29.2	39.0
145-149	34.21648672904151	35.0	34.0	37.0	29.0	39.0
150	34.15771315184974	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	18.0
21	272.0
22	2213.0
23	10795.0
24	34237.0
25	79745.0
26	152533.0
27	256277.0
28	372408.0
29	490420.0
30	627217.0
31	814510.0
32	1090583.0
33	1516341.0
34	2270864.0
35	3856368.0
36	1.0325559E7
37	2265786.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.89393561705646	18.652108949740946	15.340837051757351	32.113118381445254
2	24.595359658418108	25.490239405926108	32.64898772464892	17.265413211006866
3	19.663403590277838	29.44448913505137	29.9798867440708	20.91222053059999
4	23.30585771371462	34.318297757955015	21.64536166121938	20.730482867110993
5	25.310479715595598	35.166169607805166	21.6643634452022	17.858987231397037
6	18.870041866822653	39.26093405354729	21.867920883721993	20.001103195908062
7	20.369780012970207	20.534299378734357	37.282753994098854	21.813166614196582
8	21.381124309737025	23.24283154388833	28.277758080806574	27.09828606556807
9	23.564041143165863	23.274913606435977	27.760952591873128	25.400092658525033
10-14	23.057388698043447	28.794775436321018	25.29854401313043	22.849291852505107
15-19	23.061336368322106	27.648905791869556	26.861477160286007	22.428280679522334
20-24	22.98601697117522	27.643271464981268	26.763847219035945	22.606864344807565
25-29	23.085948478682084	27.254330768092494	26.754832130493924	22.904888622731495
30-34	22.95339365572144	27.00337066488699	26.962041784724356	23.08119389466722
35-39	23.239372987474383	26.7505079004717	27.01905375750347	22.991065354550447
40-44	23.424278718432294	26.398431988675732	27.06504784046711	23.11224145242486
45-49	23.313820917786167	26.252479914882603	27.111636970585966	23.322062196745268
50-54	23.564776882972517	26.292749462140268	26.80400669498396	23.338466959903258
55-59	23.599604935034755	26.33645439290459	26.808076023025922	23.255864649034734
60-64	23.303229243944173	26.316173961001322	26.887302537863704	23.493294257190804
65-69	23.538996196001747	26.250506907347106	26.859882367701733	23.350614528949418
70-74	23.68946946952912	26.23532285984132	26.570744072122032	23.504463598507535
75-79	23.494039100639604	26.367767611492305	26.828398662724624	23.309794625143464
80-84	23.813817969975677	26.3650143917411	26.51433067634707	23.306836961936156
85-89	23.542961245960882	26.43453124022393	26.736446083762118	23.286061430053078
90-94	23.584340609957266	26.190183691636136	26.756925140585064	23.468550557821537
95-99	23.592891412749534	26.35453836423772	26.77248740304207	23.280082819970676
100-104	23.824841653109203	26.191739803604147	26.694533963822902	23.288884579463744
105-109	23.62692496513305	26.381209552931722	26.595973574940686	23.395891906994542
110-114	23.721253594697245	26.490206632814363	26.482071287145757	23.30646848534264
115-119	23.87171693697863	26.543791498125145	26.65306665289534	22.93142491200088
120-124	23.826762324427985	26.68060020478229	26.780411709733514	22.712225761056207
125-129	23.79477062772636	26.758732907031824	26.627474933844454	22.819021531397365
130-134	23.94572127163646	26.76682907564266	26.641578684103283	22.645870968617594
135-139	23.693509831436963	26.778265732997124	26.93197245469614	22.596251980869773
140-144	23.754294850086186	26.922127279510615	26.694381463792826	22.62919640661037
145-149	23.927418812891684	27.534884329122818	26.133016956940438	22.404679901045064
150	23.66214530141166	27.54644595465592	26.08988390042403	22.70152484350839
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	1.5
9	2.5
10	2.5
11	2.5
12	4.5
13	10.0
14	18.0
15	27.5
16	47.0
17	82.0
18	140.5
19	242.5
20	395.0
21	590.5
22	908.0
23	1559.0
24	2587.0
25	4235.5
26	6626.5
27	10275.5
28	15936.0
29	23039.0
30	33047.5
31	48099.0
32	69310.5
33	95955.0
34	131937.5
35	187614.5
36	266423.5
37	363114.0
38	483600.5
39	630106.5
40	803259.0
41	990901.0
42	1182661.0
43	1378684.0
44	1532773.5
45	1624848.5
46	1650365.0
47	1615752.5
48	1563239.0
49	1505581.0
50	1392572.5
51	1228387.5
52	1043019.5
53	869343.5
54	737227.5
55	631359.0
56	514099.0
57	399880.5
58	327781.0
59	266505.5
60	197349.0
61	131730.0
62	82830.5
63	51266.5
64	31507.5
65	15940.0
66	8808.5
67	5031.5
68	3137.5
69	1967.0
70	818.5
71	521.5
72	333.5
73	256.0
74	209.5
75	125.5
76	82.0
77	38.0
78	16.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	8.276038320043671E-7
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.276038320043671E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	4.138019160021836E-6
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4166152E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.189022863715834
#Duplication Level	Percentage of total
1	25.544036800221736
2	8.479910561254947
3	5.258045959438499
4	3.8225579963334955
5	2.9505870418881397
6	2.268613938355063
7	1.8861960421910646
8	1.6511633741600775
9	1.3969676180590613
>10	17.503633323086216
>50	6.066456161146064
>100	12.435872673772073
>500	3.69882362030612
>1k	5.394430718885586
>5k	0.8991692888422775
>10k+	0.7435348820596466
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	35477	0.14680450574009465	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	24257	0.10037593076464968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.9793737952157217E-4	0.0	0.0	0.0	0.0017462440855292147	0.0
2	0.0017007258747689744	4.138019160021836E-6	0.0	4.138019160021836E-6	0.008027757170442362	0.0
3	0.002466259419373014	4.138019160021836E-6	0.0	4.138019160021836E-6	0.0117685264911021	0.0
4	0.005743570594110308	4.138019160021836E-6	0.0	4.138019160021836E-6	0.01767761785161328	0.0
5	0.005967023628751487	4.138019160021836E-6	0.0	4.138019160021836E-6	0.021455629344713217	0.0
6	0.007063598706157273	4.138019160021836E-6	0.0	4.138019160021836E-6	0.028680610798111343	0.0
7	0.00899605365388747	8.276038320043671E-6	0.0	4.138019160021836E-6	0.03806150023388084	0.0
8	0.01535205108368101	8.276038320043671E-6	0.0	4.138019160021836E-6	0.05263974173463777	0.0
9	0.01586930347868374	8.276038320043671E-6	0.0	4.138019160021836E-6	0.06239719091396926	0.0
10-11	0.016591387822107548	8.276038320043671E-6	0.0	4.138019160021836E-6	0.1044787767618113	0.0
12-13	0.01731554117511137	1.034504790005459E-5	0.0	4.138019160021836E-6	0.12304193071366926	0.0
14-15	0.020232844682926764	1.6552076640087343E-5	0.0	4.138019160021836E-6	0.13200901823343658	0.0
16-17	0.023545329020524246	1.6552076640087343E-5	0.0	4.138019160021836E-6	0.13928365591675496	0.0
18-19	0.030288231241779824	1.862108622009826E-5	0.0	4.138019160021836E-6	0.14653553449469325	0.0
20-21	0.03599662867302995	2.896613412015285E-5	0.0	4.138019160021836E-6	0.1515487447070597	0.0
22-23	0.03825598713440187	4.138019160021836E-5	0.0	4.138019160021836E-6	0.15663437025472654	0.0
24-25	0.04375541459807089	4.758722034025111E-5	0.0	4.138019160021836E-6	0.16100204947812957	0.0
26-27	0.04871689957093707	5.586325866029478E-5	0.0	4.138019160021836E-6	0.16459385010902855	0.0
28-29	0.06022886887411781	5.79322682403057E-5	0.0	4.138019160021836E-6	0.1679208175136861	0.0
30-31	0.08291142089977752	5.79322682403057E-5	0.0	4.138019160021836E-6	0.17133675233028411	0.0
32-33	0.10105870392605326	6.827731614036028E-5	0.0	4.138019160021836E-6	0.1741237082345588	0.0
34-35	0.11426519207526295	7.03463257203712E-5	0.0	4.138019160021836E-6	0.1768630769184933	0.0
36-37	0.11637351283729408	7.03463257203712E-5	0.0	4.138019160021836E-6	0.17894243154640427	0.0
38-39	0.11837631411074465	7.03463257203712E-5	0.0	4.138019160021836E-6	0.1805252238751126	0.0
40-41	0.11969841123237163	7.448434488039304E-5	0.0	4.138019160021836E-6	0.1822507778648417	2.069009580010918E-6
42-43	0.11996324445861303	8.06913736204258E-5	0.0	4.138019160021836E-6	0.184011505017431	8.276038320043671E-6
44-45	0.12077636522355731	8.689840236045854E-5	0.0	4.138019160021836E-6	0.18550739894377888	8.276038320043671E-6
46-47	0.12214604956552454	9.103642152048039E-5	0.0	4.138019160021836E-6	0.18708605325332722	1.034504790005459E-5
48-49	0.12334607512193088	9.931245984052406E-5	0.0	4.138019160021836E-6	0.18829849286721362	1.2414057480065507E-5
50-51	0.12433713071075611	9.931245984052406E-5	2.069009580010918E-6	4.138019160021836E-6	0.18957714078766036	1.2414057480065507E-5
52-53	0.12543784380732192	9.931245984052406E-5	4.138019160021836E-6	4.138019160021836E-6	0.19073371714288645	1.2414057480065507E-5
54-55	0.12602958054720503	1.158645364806114E-4	1.034504790005459E-5	8.276038320043671E-6	0.1917992570765921	1.2414057480065507E-5
56-57	0.12690684060912966	1.2000255564063323E-4	1.4483067060076425E-5	8.276038320043671E-6	0.19289376314441786	1.4483067060076425E-5
58-59	0.1270475332605704	1.2000255564063323E-4	1.6552076640087343E-5	8.276038320043671E-6	0.19380205835004266	1.6552076640087343E-5
60-61	0.1273847818221122	1.2207156522064415E-4	1.6552076640087343E-5	8.276038320043671E-6	0.19451793566472644	2.069009580010918E-5
62-63	0.12765996009625363	1.2414057480065506E-4	1.6552076640087343E-5	8.276038320043671E-6	0.1955358883780918	2.4828114960131014E-5
64-65	0.12771375434533394	1.282785939606769E-4	1.6552076640087343E-5	8.276038320043671E-6	0.19647108070825675	2.4828114960131014E-5
66-67	0.12782961888181454	1.4689968018077518E-4	1.6552076640087343E-5	8.276038320043671E-6	0.1971310947642802	2.4828114960131014E-5
68-69	0.12790617223627493	1.4896868976078609E-4	1.6552076640087343E-5	8.276038320043671E-6	0.19788214524182418	2.4828114960131014E-5
70-71	0.12798686360989536	1.531067089208079E-4	1.6552076640087343E-5	8.276038320043671E-6	0.1987014730355085	2.4828114960131014E-5
72-73	0.1280468648877157	1.531067089208079E-4	2.4828114960131014E-5	1.034504790005459E-5	0.19929320977539164	2.4828114960131014E-5
74-75	0.12807583102183584	1.551757185008188E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20000908709007542	3.3104153280174686E-5
76-77	0.12812134923259608	1.634517568208625E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20092772734360026	3.3104153280174686E-5
78-79	0.12817721249125635	1.6552076640087343E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.2019974052964659	3.3104153280174686E-5
80-81	0.12822479971159662	1.7793482388093893E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20279397398477012	4.138019160021836E-5
82-83	0.12824548980739672	1.7793482388093893E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.2036629580083747	4.138019160021836E-5
84-85	0.12828273197983692	1.882798717809935E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20453194203197927	4.138019160021836E-5
86-87	0.12834066424807722	1.9034888136100443E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.205423685160964	4.138019160021836E-5
88-89	0.12839652750673752	1.9034888136100443E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20623266790674824	4.758722034025111E-5
90-91	0.12840894156421762	1.9655591010103718E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20684302573285146	4.965622992026203E-5
92-93	0.1284254936408577	2.0276293884106993E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.2073851062428143	5.3794249080283865E-5
94-95	0.12845859779413787	2.0690095800109177E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20808856950001803	6.0001277820316615E-5
96-97	0.12846687383245792	2.0690095800109177E-4	2.6897124540141932E-5	1.4483067060076425E-5	0.20868651326864118	6.207028740032753E-5
98-99	0.12848135689951798	2.0896996758110268E-4	2.896613412015285E-5	1.6552076640087343E-5	0.20926583595104425	6.620830656034937E-5
100-101	0.12851653006237818	2.1517699632113543E-4	2.896613412015285E-5	1.6552076640087343E-5	0.2097355011257067	6.620830656034937E-5
102-103	0.12853722015817826	2.1931501548115727E-4	3.1035143700163765E-5	1.6552076640087343E-5	0.21025689153986948	6.827731614036028E-5
104-105	0.12856204827313839	2.1931501548115727E-4	3.3104153280174686E-5	1.6552076640087343E-5	0.21081345511689242	7.241533530038212E-5
106-107	0.12859515242641856	2.2345303464117912E-4	3.3104153280174686E-5	1.6552076640087343E-5	0.2113596736460153	7.448434488039304E-5
108-109	0.12862411856053874	2.3586709212124462E-4	3.724217244019652E-5	1.6552076640087343E-5	0.21190382316555817	7.448434488039304E-5
110-111	0.12866342974255893	2.3586709212124462E-4	3.724217244019652E-5	1.6552076640087343E-5	0.21244383466594102	7.448434488039304E-5
112-113	0.128673774790459	2.3586709212124462E-4	3.724217244019652E-5	1.6552076640087343E-5	0.21308936565490444	7.448434488039304E-5
114-115	0.12870274092457912	2.4000511128126646E-4	3.724217244019652E-5	1.6552076640087343E-5	0.21365627427982742	7.655335446040396E-5
116-117	0.12872963804911927	2.441431304412883E-4	3.9311182020207436E-5	1.6552076640087343E-5	0.21416731964609012	8.06913736204258E-5
118-119	0.12872963804911927	2.4621214002129924E-4	4.138019160021836E-5	1.6552076640087343E-5	0.21466181293571274	8.482939278044762E-5
120-121	0.1287337760682793	2.482811496013101E-4	4.138019160021836E-5	1.6552076640087343E-5	0.21521837651273568	8.689840236045854E-5
122-123	0.1287358450778593	2.482811496013101E-4	4.138019160021836E-5	1.6552076640087343E-5	0.21572321485025833	9.103642152048039E-5
124-125	0.12874412111617933	2.482811496013101E-4	4.138019160021836E-5	2.069009580010918E-5	0.21629633050392136	9.310543110049131E-5
126-127	0.12876274220239944	2.52419168761332E-4	4.138019160021836E-5	2.2759105380120097E-5	0.21697082762700493	9.724345026051314E-5
128-129	0.1287730872502995	2.52419168761332E-4	4.138019160021836E-5	2.4828114960131014E-5	0.21757911644352812	9.931245984052406E-5
130-131	0.12878550130777955	2.689712454014193E-4	4.138019160021836E-5	2.4828114960131014E-5	0.21818533625047132	9.931245984052406E-5
132-133	0.12881860546105978	2.689712454014193E-4	4.138019160021836E-5	2.4828114960131014E-5	0.21882672922027468	9.931245984052406E-5
134-135	0.12882895050895982	2.7104025498143024E-4	4.138019160021836E-5	2.6897124540141932E-5	0.21942467298889787	9.931245984052406E-5
136-137	0.1288455025855999	2.77247283721463E-4	4.344920118022928E-5	2.896613412015285E-5	0.2199626154797007	9.931245984052406E-5
138-139	0.12885584763349994	2.8345431246149574E-4	4.551821076024019E-5	2.896613412015285E-5	0.220581249344124	9.931245984052406E-5
>>END_MODULE
