##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.R.1.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24185942
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.845449104277186	36.0	34.0	38.0	30.0	39.0
2	34.70786583379717	35.0	34.0	38.0	31.0	39.0
3	34.8591030690473	35.0	34.0	38.0	31.0	39.0
4	34.831818334799614	36.0	34.0	38.0	31.0	39.0
5	34.695115369085066	35.0	34.0	37.0	31.0	39.0
6	34.69061329097705	35.0	34.0	37.0	31.0	39.0
7	34.73029253936026	35.0	34.0	37.0	32.0	39.0
8	34.83674338588921	35.0	34.0	37.0	31.0	39.0
9	34.79355594253885	35.0	34.0	37.0	31.0	39.0
10-14	34.77520383535196	35.0	34.0	37.0	31.0	39.0
15-19	34.79383683298339	35.0	34.0	37.0	31.0	39.0
20-24	34.7737850772982	35.0	34.0	37.0	31.0	39.0
25-29	34.770015788510534	35.0	34.0	37.0	31.0	39.0
30-34	34.74911859955672	35.0	34.0	37.0	31.0	39.0
35-39	34.74006613428578	35.0	34.0	37.0	31.0	39.0
40-44	34.691867540243	35.0	34.0	37.0	31.0	39.0
45-49	34.59179474588999	35.0	34.0	37.0	30.8	39.0
50-54	34.560258715579494	35.0	34.0	37.0	30.0	39.0
55-59	34.54573336031319	35.0	34.0	37.0	30.0	39.0
60-64	34.51736677446758	35.0	34.0	37.0	30.0	39.0
65-69	34.47913014924124	35.0	34.0	37.0	30.0	39.0
70-74	34.47894597613771	35.0	34.0	37.0	30.0	39.0
75-79	34.461106811551936	35.0	34.0	37.0	30.0	39.0
80-84	34.44499081325838	35.0	34.0	37.0	30.0	39.0
85-89	34.42318116863093	35.0	34.0	37.0	30.0	39.0
90-94	34.39801887393925	35.0	34.0	37.0	30.0	39.0
95-99	34.38143127937708	35.0	34.0	37.0	30.0	39.0
100-104	34.339206792110886	35.0	34.0	37.0	30.0	39.0
105-109	34.31195802917249	35.0	34.0	37.0	30.0	39.0
110-114	34.27567125564099	35.0	34.0	37.0	30.0	39.0
115-119	34.23573183959508	35.0	34.0	37.0	30.0	39.0
120-124	34.194545434699215	35.0	34.0	37.0	29.2	39.0
125-129	34.180353595489485	35.0	34.0	37.0	29.0	39.0
130-134	34.12482579342992	35.0	34.0	37.0	29.0	39.0
135-139	34.088800891030004	35.0	34.0	37.0	29.0	39.0
140-144	34.038459614266834	35.0	34.0	37.0	29.0	39.0
145-149	34.00315302997088	35.0	34.0	37.0	29.0	39.0
150	33.98799480293139	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	15.0
22	353.0
23	3272.0
24	21681.0
25	85919.0
26	218629.0
27	397602.0
28	585318.0
29	765041.0
30	957825.0
31	1201279.0
32	1537866.0
33	2016803.0
34	2767596.0
35	4258460.0
36	8216192.0
37	1152081.0
38	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.78645869769828	10.424843946369448	14.74984863091396	38.03884872501831
2	22.519792917566434	11.87729689384619	36.571726596015104	29.031183592572273
3	21.462953561921925	16.37969471216704	28.12407568508948	34.03327604082156
4	26.7077630239706	24.107721669336332	22.2283350525841	26.95618025410897
5	26.355054140146088	30.00170264253595	24.38447487700909	19.258768340308876
6	19.80272418659853	36.42844487922422	25.380879424343117	18.387951509834124
7	16.110337038503914	25.241976420093152	40.19104743166596	18.456639109736976
8	18.615131791935525	24.573216671546916	31.4844591850649	25.32719235145266
9	19.605486313963794	22.879112892459084	32.91754474264768	24.597856050929447
10-14	20.29362239996724	29.19480612568969	27.175985810050342	23.335585664292726
15-19	20.501480912334483	27.795541018822306	27.87778480005062	23.825193268792592
20-24	20.639404188169046	28.001250811888635	27.58804213580554	23.771302864136782
25-29	20.63387184092447	28.183306997884156	27.459182755073115	23.723638406118262
30-34	20.59626775982624	27.89344892733047	27.517823344671594	23.992459968171694
35-39	20.68353622461381	27.868396781447085	27.531523995617086	23.916542998322015
40-44	20.712931194692896	27.963871820960733	27.457899491851194	23.865297492495174
45-49	20.80508095696052	27.743297679806062	27.415148642428473	24.036472720804948
50-54	20.91839480430284	27.72931018611456	27.501926658091097	23.850368351491504
55-59	20.92221747556576	27.772830775123836	27.41814497690213	23.886806772408274
60-64	20.86435398255789	27.688195990575636	27.403683181466786	24.043766845399688
65-69	20.960993387762127	27.631851565370212	27.478117963704495	23.92903708316316
70-74	21.085110148845786	27.570863297359082	27.45379173788725	23.890234815907878
75-79	21.085279075564976	27.480162232295914	27.46148772443634	23.973070967702775
80-84	21.14085921053253	27.48407579607086	27.42325197439472	23.951813019001893
85-89	21.1162346518461	27.475944290864284	27.489464552887128	23.918356504402492
90-94	21.1030208625213	27.39332289667698	27.437043398868603	24.066612841933114
95-99	21.18590007066839	27.300542028589707	27.566907751013048	23.94665014972885
100-104	21.236581420139924	27.377847771107263	27.408390329915783	23.977180478837035
105-109	21.2084112647003	27.21265963762335	27.524422868510324	24.05450622916603
110-114	21.293828393819766	27.19616315118209	27.5597555253073	23.950252929690844
115-119	21.336522636540415	27.228596883275628	27.524969185804498	23.90991129437946
120-124	21.30061811007155	27.162094830153816	27.513947495763524	24.023339564011106
125-129	21.328292593001493	27.078444348662355	27.574224575025646	24.019038483310506
130-134	21.409651635105416	27.193896551354875	27.46439345548505	23.932058358054658
135-139	21.400916982618924	27.07405273200792	27.557141810962115	23.96788847441104
140-144	21.451032126985904	27.002175521147027	27.674138132134594	23.87265421973247
145-149	21.44825081644371	27.431373383567387	27.423525018641627	23.696850781347273
150	21.32272297533698	27.029252466909888	27.535555181968224	24.112469375784908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	1.5
9	2.0
10	3.0
11	5.5
12	8.5
13	15.5
14	30.0
15	73.5
16	152.5
17	296.0
18	585.0
19	1080.0
20	2046.5
21	3560.0
22	5648.0
23	8946.0
24	13996.0
25	21526.5
26	31824.5
27	44843.0
28	61149.0
29	85311.5
30	115844.0
31	147608.0
32	178058.0
33	212380.0
34	250282.5
35	296001.0
36	368188.5
37	466294.5
38	584749.5
39	732307.0
40	901594.5
41	1070324.5
42	1243872.5
43	1424040.5
44	1573740.0
45	1667025.0
46	1695642.5
47	1661096.5
48	1576412.5
49	1450262.0
50	1286196.5
51	1103963.5
52	921706.5
53	741989.5
54	575451.0
55	434804.0
56	327384.0
57	246974.0
58	184681.0
59	135104.0
60	95217.5
61	68705.5
62	49789.0
63	33011.5
64	21860.0
65	16559.5
66	15777.5
67	11913.0
68	5984.0
69	3720.0
70	3645.5
71	2669.0
72	1215.0
73	428.5
74	166.0
75	95.5
76	54.0
77	31.0
78	12.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0750046452604575E-4
2	7.442339851803167E-5
3	0.1640622473997498
4	7.855803176903342E-5
5	3.307706600801407E-5
6	2.894243275701232E-5
7	4.548096576101935E-5
8	6.615413201602815E-5
9	5.375023226302287E-5
10-14	1.4553909043526193E-4
15-19	1.670391833404711E-4
20-24	2.1086629580108974E-4
25-29	1.5380835693726546E-4
30-34	2.042508825994869E-4
35-39	6.946183861682956E-5
40-44	1.4223138383446054E-4
45-49	1.7117381659147285E-4
50-54	8.76542249212373E-5
55-59	1.1825051097865033E-4
60-64	1.496737236862637E-4
65-69	0.06540741725089723
70-74	2.1003936915088938E-4
75-79	1.2486592418025315E-4
80-84	9.675041807344119E-5
85-89	8.351959167023555E-5
90-94	1.4471216378506157E-4
95-99	9.178885817223906E-5
100-104	7.525032516823202E-5
105-109	9.840427137384188E-5
110-114	4.6307892411219706E-5
115-119	4.6307892411219706E-5
120-124	9.592349142324081E-5
125-129	9.840427137384188E-5
130-134	3.721169925901584E-5
135-139	3.8038625909216184E-5
140-144	5.375023226302287E-5
145-149	5.209637896262217E-5
150	6.615413201602815E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4185942E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.19676833916521
#Duplication Level	Percentage of total
1	40.224785719077786
2	9.947834881228596
3	6.188729524312605
4	4.271373312991016
5	3.2340220621070914
6	2.6037224354423234
7	2.1812935087417955
8	1.7755868212394954
9	1.4836716468891524
>10	18.071254365245363
>50	4.506444253033683
>100	4.733245981003184
>500	0.4346837806282115
>1k	0.34335170805944115
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.0673166255008797E-5	0.0	8.26926650200352E-6	1.240389975300528E-4	1.653853300400704E-5
2	0.0	1.4884679703606335E-4	0.0	1.2403899753005279E-5	0.0017324113321697374	2.0673166255008797E-5
3	8.26926650200352E-6	1.6125069678906863E-4	0.0	1.2403899753005279E-5	0.0026585691803941314	7.028876526702991E-5
4	2.0673166255008797E-5	1.736545965420739E-4	0.0	1.653853300400704E-5	0.003915497688698666	9.923119802404223E-5
5	2.4807799506010558E-5	1.736545965420739E-4	0.0	1.653853300400704E-5	0.005069060365728157	1.2817363078105454E-4
6	4.548096576101936E-5	1.736545965420739E-4	0.0	1.653853300400704E-5	0.006173007443745627	1.3644289728305807E-4
7	5.788486551402464E-5	2.1086629580108974E-4	0.0	1.653853300400704E-5	0.007347243287030127	1.4057753053405982E-4
8	6.615413201602816E-5	2.1086629580108974E-4	0.0	1.653853300400704E-5	0.009174751183972905	1.4884679703606335E-4
9	7.028876526702991E-5	2.1086629580108974E-4	0.0	1.653853300400704E-5	0.012106206158933153	1.529814302870651E-4
10-11	1.0336583127504398E-4	2.253375121795959E-4	0.0	2.06731662550088E-5	0.016166416011416877	2.0879897917558887E-4
12-13	1.633180134145695E-4	2.3153946205609855E-4	0.0	2.687511613151144E-5	0.019732537190405897	2.3774141193260117E-4
14-15	1.8812581292058006E-4	2.3774141193260117E-4	0.0	2.894243275701232E-5	0.02377620850988562	2.501453116856065E-4
16-17	2.0879897917558887E-4	2.4187604518360293E-4	0.0	2.894243275701232E-5	0.028109304156935463	2.6461652806411264E-4
18-19	2.356740953071003E-4	2.480779950601056E-4	0.0	2.894243275701232E-5	0.03178499311707603	2.666838446896135E-4
20-21	2.852896943191214E-4	2.894243275701232E-4	0.0	3.10097493825132E-5	0.035100968984379435	2.770204278171179E-4
22-23	3.576457762116522E-4	3.059628605741302E-4	0.0	3.927901588451671E-5	0.038491368250200886	2.894243275701232E-4
24-25	3.969247920961689E-4	3.080301771996311E-4	0.0	4.1346332510017594E-5	0.04241100057215055	2.956262774466258E-4
26-27	4.5480965761019354E-4	3.1009749382513195E-4	0.0	4.3413649135518476E-5	0.046307892411219714	3.10097493825132E-4
28-29	4.940886734947103E-4	3.1009749382513195E-4	0.0	5.788486551402464E-5	0.05044666029547247	3.204340769526364E-4
30-31	5.561081722597367E-4	3.1009749382513195E-4	0.0	6.822144864152903E-5	0.054473793081948184	3.5351114296065046E-4
32-33	5.912525548932516E-4	3.1423212707613375E-4	0.0	7.442339851803167E-5	0.05812674155920824	3.927901588451672E-4
34-35	6.284642541522675E-4	3.1423212707613375E-4	0.0	7.442339851803167E-5	0.06144065010988615	4.051940585981724E-4
36-37	6.36733520654271E-4	3.204340769526364E-4	0.0	7.442339851803167E-5	0.06473802012756005	4.2793454147868214E-4
38-39	6.594740035347807E-4	3.3077066008014075E-4	0.0	7.855803176903344E-5	0.06845919005346163	4.4860770773369094E-4
40-41	7.152915524233044E-4	3.3077066008014075E-4	0.0	8.062534839453431E-5	0.07132035626315486	4.6307892411219704E-4
42-43	7.235608189253079E-4	3.349052933311425E-4	0.0	9.096193152203872E-5	0.07436964828576866	4.7341550723970145E-4
44-45	7.40099351929315E-4	3.411072432076452E-4	0.0	9.096193152203872E-5	0.07807221236204073	4.796174571162041E-4
46-47	7.711091013118281E-4	3.473091930841478E-4	0.0	9.509656477304047E-5	0.08171689157279878	4.8375209036720585E-4
48-49	8.021188506943415E-4	3.5144382633514956E-4	0.0	1.012985146495431E-4	0.08504113670660418	4.940886734947103E-4
50-51	8.186573836983485E-4	3.5971309283715306E-4	0.0	1.03365831275044E-4	0.08879124906526278	5.044252566222148E-4
52-53	8.972154154673819E-4	3.7211699259015837E-4	0.0	1.03365831275044E-4	0.09251448630778987	5.085598898732165E-4
54-55	9.509656477304047E-4	3.7211699259015837E-4	0.0	1.03365831275044E-4	0.09546661444900512	5.085598898732165E-4
56-57	9.654368641089108E-4	3.762516258411601E-4	0.0	1.0750046452604575E-4	0.09927461167317775	5.230311062517226E-4
58-59	9.881773469894205E-4	3.9899210872166983E-4	0.0	1.1576973102804927E-4	0.10297510843282433	5.292330561282252E-4
60-61	0.0010109178298699302	4.13463325100176E-4	0.0	1.1576973102804927E-4	0.10599545802268111	5.33367689379227E-4
62-63	0.0010253890462484363	4.30001858104183E-4	0.0	1.1576973102804927E-4	0.10903854809541841	5.33367689379227E-4
64-65	0.00103365831275044	4.30001858104183E-4	0.0	1.1990436427905103E-4	0.11339645154197425	5.33367689379227E-4
66-67	0.001050196845754447	4.5274234098469264E-4	0.0	1.240389975300528E-4	0.11735122824655744	5.375023226302288E-4
68-69	0.00108120659513696	4.713481906142006E-4	0.0	1.2817363078105454E-4	0.11991883549542953	5.561081722597367E-4
70-71	0.0011411587772764856	4.8375209036720585E-4	0.0	1.2817363078105454E-4	0.12237687496315008	5.685120720127419E-4
72-73	0.0011763031599100007	4.8788672361820766E-4	0.0	1.3230826403205632E-4	0.1250251075604167	5.726467052637437E-4
74-75	0.0011907743762885068	4.961559901202112E-4	0.0	1.5711606353806688E-4	0.12789661035323743	5.747140218892446E-4
76-77	0.0012155821757945172	5.044252566222148E-4	0.0	1.653853300400704E-4	0.13026988983931242	5.747140218892446E-4
78-79	0.0012486592418025314	5.126945231242182E-4	0.0	1.8192386304407743E-4	0.13262869810900896	5.747140218892446E-4
80-81	0.0012858709410615472	5.126945231242182E-4	0.0	1.8605849629507918E-4	0.1351115453762355	5.767813385147455E-4
82-83	0.00133962117332457	5.209637896262217E-4	0.0	1.8605849629507918E-4	0.13742073804691998	5.788486551402464E-4
84-85	0.0014078426219660991	5.33367689379227E-4	0.0	1.8605849629507918E-4	0.1395624780709389	5.788486551402464E-4
86-87	0.001461592854229122	5.354350060047279E-4	0.0	1.8605849629507918E-4	0.1414726786329017	5.85050605016749E-4
88-89	0.001496737236862637	5.395696392557297E-4	0.0	1.8605849629507918E-4	0.1435317259919006	5.871179216422499E-4
90-91	0.0015091411366156423	5.499062223832341E-4	0.0	1.8605849629507918E-4	0.14602904447550566	5.933198715187525E-4
92-93	0.0015442855192491572	5.519735390087349E-4	0.0	1.8812581292058006E-4	0.1484994878429792	6.015891380207561E-4
94-95	0.0015959684348866793	5.561081722597367E-4	0.0	1.922604461715818E-4	0.15127796138765237	6.036564546462569E-4
96-97	0.001684863049783217	5.643774387617402E-4	0.0	1.943277627970827E-4	0.15348172091043633	6.036564546462569E-4
98-99	0.0017551518150502468	5.809159717657473E-4	0.0	1.943277627970827E-4	0.1553340366068851	6.036564546462569E-4
100-101	0.0018192386304407742	5.829832883912481E-4	0.0	1.943277627970827E-4	0.15726284301847743	6.036564546462569E-4
102-103	0.0018461137465722857	5.871179216422499E-4	0.0	1.943277627970827E-4	0.1588670807198661	6.036564546462569E-4
104-105	0.001877123495954799	5.933198715187525E-4	0.0	1.963950794225836E-4	0.16044030867187226	6.057237712717578E-4
106-107	0.00192673909496682	5.995218213952552E-4	0.0	1.9846239604808446E-4	0.16193911322536042	6.098584045227595E-4
108-109	0.0020342395594928654	6.015891380207561E-4	0.0	2.025970292990862E-4	0.1635536875098766	6.119257211482604E-4
110-111	0.0021148649078874	6.098584045227595E-4	0.0	2.025970292990862E-4	0.16500080914772722	6.119257211482604E-4
112-113	0.002197557572907435	6.181276710247631E-4	0.0	2.025970292990862E-4	0.16650581565109185	6.119257211482604E-4
114-115	0.0022843848711784722	6.243296209012657E-4	0.0	2.1086629580108974E-4	0.16819894796737708	6.119257211482604E-4
116-117	0.0023464043699434984	6.305315707777683E-4	0.0	2.2740482880509677E-4	0.16973703153674974	6.119257211482604E-4
118-119	0.002408423868708525	6.53272053658278E-4	0.0	2.3360677868159942E-4	0.1710621814936958	6.119257211482604E-4
120-121	0.0025014531168560646	6.574066869092798E-4	0.0	2.3980872855810205E-4	0.1724989665484189	6.119257211482604E-4
122-123	0.0026213574811351156	6.574066869092798E-4	0.0	2.4394336180910383E-4	0.17372075067408993	6.119257211482604E-4
124-125	0.0027309252622866624	6.594740035347807E-4	0.0	2.460106784346047E-4	0.1750810450136695	6.119257211482604E-4
126-127	0.0027784735446731825	6.615413201602815E-4	0.0	2.480779950601056E-4	0.17636691595473106	6.119257211482604E-4
128-129	0.002854964259816715	6.615413201602815E-4	0.0	2.563472615621091E-4	0.17759490203027856	6.160603543992622E-4
130-131	0.002900445225577734	6.615413201602815E-4	0.0	2.6254921143861173E-4	0.17889110955446763	6.181276710247631E-4
132-133	0.002958330091091759	6.656759534112832E-4	0.0	2.6461652806411264E-4	0.18010875904688767	6.222623042757648E-4
134-135	0.003059628605741302	6.69810586662285E-4	0.0	2.6461652806411264E-4	0.18108453249412407	6.243296209012657E-4
136-137	0.0032229466191558715	6.739452199132868E-4	0.0	2.6461652806411264E-4	0.18214920055625702	6.243296209012657E-4
138-139	0.003318043183928912	6.760125365387877E-4	0.0	2.6461652806411264E-4	0.1832779554337805	6.243296209012657E-4
>>END_MODULE
