##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24185942
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.487834461853915	36.0	35.0	38.0	32.0	39.0
2	35.13403265417572	35.0	34.0	37.0	32.0	39.0
3	35.29444472330249	35.0	34.0	37.0	33.0	39.0
4	35.34118749643905	36.0	34.0	37.0	33.0	39.0
5	35.25294830360546	35.0	34.0	37.0	32.0	39.0
6	35.22093449988427	35.0	34.0	37.0	33.0	39.0
7	35.26606517951627	35.0	34.0	37.0	33.0	39.0
8	35.39889965832218	36.0	34.0	38.0	33.0	39.0
9	35.36774412177123	36.0	34.0	38.0	33.0	39.0
10-14	35.32896705036339	35.4	34.0	37.4	32.4	39.0
15-19	35.30000312578274	35.0	34.0	37.0	32.2	39.0
20-24	35.26525209561819	35.0	34.0	37.0	32.0	39.0
25-29	35.254330519770534	35.0	34.0	37.0	32.0	39.0
30-34	35.23640629750952	35.0	34.0	37.0	32.0	39.0
35-39	35.21007476161152	35.0	34.0	37.0	32.0	39.0
40-44	35.1880463948851	35.0	34.0	37.0	32.0	39.0
45-49	35.1589761606143	35.0	34.0	37.0	32.0	39.0
50-54	35.13404390864743	35.0	34.0	37.0	32.0	39.0
55-59	35.10254045924695	35.0	34.0	37.0	32.0	39.0
60-64	35.06107060870319	35.0	34.0	37.0	32.0	39.0
65-69	35.02391957278323	35.0	34.0	37.0	32.0	39.0
70-74	35.000645945483534	35.0	34.0	37.0	32.0	39.0
75-79	34.94970472516638	35.0	34.0	37.0	31.0	39.0
80-84	34.87103131232185	35.0	34.0	37.0	31.0	39.0
85-89	34.855378756800135	35.0	34.0	37.0	31.0	39.0
90-94	34.80405676156836	35.0	34.0	37.0	31.0	39.0
95-99	34.73825409818646	35.0	34.0	37.0	31.0	39.0
100-104	34.66865341858505	35.0	34.0	37.0	30.4	39.0
105-109	34.60959735204856	35.0	34.0	37.0	30.0	39.0
110-114	34.54226424589954	35.0	34.0	37.0	30.0	39.0
115-119	34.482862077482864	35.0	34.0	37.0	30.0	39.0
120-124	34.4080702004495	35.0	34.0	37.0	30.0	39.0
125-129	34.32529898566697	35.0	34.0	37.0	29.6	39.0
130-134	34.24702383723569	35.0	34.0	37.0	29.0	39.0
135-139	34.156977478900764	35.0	34.0	37.0	29.0	39.0
140-144	34.05419263802088	35.0	34.0	37.0	29.0	39.0
145-149	33.94698056416409	35.0	33.8	37.0	28.0	39.0
150	33.880631980346266	35.0	33.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	30.0
21	351.0
22	2786.0
23	13001.0
24	41256.0
25	95264.0
26	183935.0
27	305065.0
28	437999.0
29	571343.0
30	724764.0
31	935653.0
32	1233166.0
33	1692216.0
34	2491008.0
35	4134700.0
36	9870877.0
37	1452521.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.2570396992203	18.928674307110896	16.00733252429583	31.806953469372974
2	23.816306183153834	25.98558699925767	33.26798683301234	16.930119984576166
3	19.640764870766663	29.639780001126276	30.226104073184327	20.49335105492273
4	23.237453393380335	34.158053467588736	22.53160947793557	20.072883661095357
5	24.918967390230243	35.16157857320587	22.719602982592118	17.199851053971766
6	18.72368667716147	38.975190629333355	23.059680702120264	19.241441991384914
7	20.045867967433313	20.553055159067195	38.40456575972935	20.99651111377014
8	21.459734750046124	23.28403417158612	28.44556147533968	26.810669603028074
9	23.40779614868836	23.14798819909516	28.834130173635575	24.610085478580903
10-14	22.961242526753765	29.167035958326537	25.77055630084617	22.101165214073532
15-19	22.787716103842474	28.279102794507654	27.244539824001894	21.68864127764798
20-24	22.907268951188172	28.309412499912835	27.149217743263478	21.63410080563552
25-29	23.036943526946356	28.24582643917694	27.058749251941478	21.658480781935225
30-34	23.04141802705059	28.037459115712753	27.231093996669635	21.69002886056702
35-39	23.145413976433087	28.00513703373638	27.197460409026036	21.651988580804503
40-44	23.294654390554644	27.763264296259372	27.307397826390222	21.63468348679576
45-49	23.3182127038922	27.588249405377717	27.37569452535692	21.717843365373156
50-54	23.407984248171672	27.56858637250658	27.319194386874727	21.704234992447017
55-59	23.59192459818187	27.43117386124551	27.2125832436049	21.76431829696772
60-64	23.515049362145994	27.41508352248591	27.258035266933163	21.811831848434927
65-69	23.612143781705917	27.335143696284398	27.213386189382245	21.83932633262744
70-74	23.68503157743453	27.292118702674472	27.111948751055472	21.910900968835534
75-79	23.624173910613035	27.340736201219702	27.17382684536331	21.861263042803955
80-84	23.748266110801577	27.39022841847303	27.025234259205387	21.836271211520007
85-89	23.790102097989024	27.313010629282257	27.074556402633505	21.822330870095215
90-94	23.726575545413944	27.251151102570244	27.119424994899934	21.902848357115882
95-99	23.800537518861166	27.367014276309764	27.108643525234616	21.72380467959445
100-104	23.97408378801206	27.335663833147372	26.916034942943302	21.774217435897267
105-109	23.75722806248357	27.483419087005167	27.010540255161448	21.74881259534981
110-114	23.806082062050756	27.47801677519941	26.92724310676012	21.788658055989714
115-119	23.883639512573048	27.47491745411446	26.938801887476615	21.702641145835873
120-124	23.843722936241228	27.377844534647437	27.121511330838384	21.656921198272947
125-129	23.826087071572402	27.468934639800263	27.062214074605816	21.64276421402152
130-134	23.888824342669803	27.358958356883516	27.14084239514012	21.611374905306562
135-139	23.796238327206773	27.38073381636324	27.235511438835008	21.587516417594983
140-144	23.83481114773202	27.487073275872405	27.202358295575173	21.475757280820403
145-149	23.914571475800848	27.88059872418001	26.828266572028515	21.376563227990623
150	23.5600540181565	27.862077896325065	26.98587882167252	21.591989263845914
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	10.0
3	29.0
4	43.0
5	43.5
6	36.0
7	23.0
8	14.5
9	15.0
10	14.0
11	10.5
12	9.0
13	14.0
14	28.0
15	42.0
16	74.5
17	147.5
18	223.5
19	355.5
20	577.0
21	880.5
22	1435.0
23	2535.0
24	4155.0
25	6755.5
26	10386.5
27	16642.5
28	28339.0
29	43346.0
30	57368.0
31	74861.5
32	100440.5
33	134529.5
34	181432.5
35	251658.5
36	353501.5
37	476424.5
38	621406.0
39	808858.5
40	1028257.0
41	1232406.0
42	1411502.0
43	1575732.0
44	1694612.0
45	1745767.5
46	1744004.5
47	1689092.0
48	1583790.0
49	1438952.5
50	1252468.5
51	1052470.0
52	859971.5
53	685088.5
54	536623.0
55	407678.0
56	302184.0
57	223872.5
58	164002.0
59	116955.5
60	81590.5
61	55994.0
62	38523.5
63	26164.5
64	17264.0
65	11753.0
66	8289.5
67	6072.5
68	5256.5
69	4093.0
70	2623.0
71	2087.0
72	1303.5
73	945.5
74	834.5
75	547.5
76	345.0
77	120.5
78	31.5
79	5.0
80	1.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.134633251001759E-6
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.03322508587839994
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	1.653853300400704E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0655612256078345
85-89	1.653853300400704E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.03522128681198359
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4185942E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.814856630930194
#Duplication Level	Percentage of total
1	28.809342887578556
2	10.238461421639327
3	6.800066683066369
4	5.01934201112496
5	3.7419081466113777
6	3.039496258743707
7	2.4775370861651798
8	2.1227541067639284
9	1.8752798310854115
>10	21.267961212648867
>50	5.494902884114577
>100	6.755407539784832
>500	1.2163918549301227
>1k	1.0400147040200605
>5k	0.0541477414816533
>10k+	0.04698563024099165
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.13463325100176E-6	8.26926650200352E-6	4.13463325100176E-6	0.0	0.0014553909043526195	0.0
2	2.0673166255008797E-5	1.2403899753005279E-5	4.13463325100176E-6	0.0	0.006036564546462569	0.0
3	5.375023226302287E-5	1.2403899753005279E-5	4.13463325100176E-6	4.13463325100176E-6	0.007876476343158353	0.0
4	1.03365831275044E-4	1.2403899753005279E-5	4.13463325100176E-6	4.13463325100176E-6	0.009216097516482922	0.0
5	1.0750046452604575E-4	1.2403899753005279E-5	4.13463325100176E-6	4.13463325100176E-6	0.010894758616389636	0.0
6	1.240389975300528E-4	1.653853300400704E-5	4.13463325100176E-6	4.13463325100176E-6	0.012581688982798354	0.0
7	1.3230826403205632E-4	3.307706600801408E-5	4.13463325100176E-6	4.13463325100176E-6	0.014533235877271185	0.0
8	2.06731662550088E-4	3.307706600801408E-5	4.13463325100176E-6	8.26926650200352E-6	0.020197683431143594	0.0
9	2.06731662550088E-4	4.1346332510017594E-5	4.13463325100176E-6	8.26926650200352E-6	0.026197036278347147	0.0
10-11	2.356740953071003E-4	4.3413649135518476E-5	4.13463325100176E-6	8.26926650200352E-6	0.032750429981184936	0.0
12-13	2.646165280641126E-4	4.9615599012021116E-5	4.13463325100176E-6	8.26926650200352E-6	0.03883247549340853	0.0
14-15	2.9149164419562404E-4	6.615413201602816E-5	4.13463325100176E-6	8.26926650200352E-6	0.04731260829121313	4.13463325100176E-6
16-17	3.1009749382513195E-4	6.615413201602816E-5	4.13463325100176E-6	8.26926650200352E-6	0.05302667144409756	4.13463325100176E-6
18-19	3.638477260881548E-4	6.615413201602816E-5	4.13463325100176E-6	1.03365831275044E-5	0.06081011853910838	4.13463325100176E-6
20-21	3.9899210872166983E-4	7.442339851803167E-5	4.13463325100176E-6	1.2403899753005279E-5	0.06704514548161902	4.13463325100176E-6
22-23	4.3827112460618654E-4	9.509656477304047E-5	4.13463325100176E-6	1.2403899753005279E-5	0.07389830009515444	4.13463325100176E-6
24-25	4.920213568692094E-4	9.509656477304047E-5	4.13463325100176E-6	1.2403899753005279E-5	0.07904178385940064	4.13463325100176E-6
26-27	5.354350060047279E-4	1.0336583127504398E-4	4.13463325100176E-6	1.2403899753005279E-5	0.08320329222653391	4.13463325100176E-6
28-29	6.305315707777683E-4	1.0750046452604575E-4	4.13463325100176E-6	1.2403899753005279E-5	0.08729451182840015	4.13463325100176E-6
30-31	8.021188506943415E-4	1.1163509777704751E-4	1.2403899753005279E-5	1.2403899753005279E-5	0.09264886188844743	4.13463325100176E-6
32-33	9.6336954748341E-4	1.1576973102804927E-4	1.447121637850616E-5	1.2403899753005279E-5	0.09816446264528378	8.26926650200352E-6
34-35	0.0010894758616389636	1.1576973102804927E-4	1.653853300400704E-5	1.447121637850616E-5	0.10139774584756715	8.26926650200352E-6
36-37	0.0011452934105274874	1.1576973102804927E-4	1.860584962950792E-5	1.653853300400704E-5	0.10477780853026109	8.26926650200352E-6
38-39	0.00122178412567102	1.2197168090455191E-4	2.0673166255008797E-5	1.653853300400704E-5	0.10844522822389965	8.26926650200352E-6
40-41	0.001275534357934043	1.3024094740655544E-4	2.0673166255008797E-5	1.653853300400704E-5	0.11132913491647338	1.03365831275044E-5
42-43	0.0012941402075635506	1.426448471595607E-4	2.0673166255008797E-5	1.653853300400704E-5	0.1142171762422981	1.2403899753005279E-5
44-45	0.0013210153236950621	1.653853300400704E-4	2.0673166255008797E-5	1.653853300400704E-5	0.1165553113457396	1.447121637850616E-5
46-47	0.001376832872583586	1.943277627970827E-4	2.0673166255008797E-5	1.653853300400704E-5	0.11900094691370713	1.653853300400704E-5
48-49	0.001426448471595607	2.1086629580108974E-4	2.0673166255008797E-5	1.653853300400704E-5	0.12138869761616067	1.653853300400704E-5
50-51	0.0014760640706076283	2.1706824567759237E-4	2.0673166255008797E-5	1.653853300400704E-5	0.12358005323919159	1.653853300400704E-5
52-53	0.0015215450363686477	2.2327019555409502E-4	2.0673166255008797E-5	1.653853300400704E-5	0.12584169762748956	1.653853300400704E-5
54-55	0.0015566894190021624	2.3153946205609852E-4	2.0673166255008797E-5	1.653853300400704E-5	0.1278428601209744	2.0673166255008797E-5
56-57	0.0016269781842691924	2.4187604518360293E-4	2.0673166255008797E-5	1.860584962950792E-5	0.12985849383083775	2.0673166255008797E-5
58-59	0.001684863049783217	2.5221262831110733E-4	2.2740482880509676E-5	2.0673166255008797E-5	0.13168600172778056	2.0673166255008797E-5
60-61	0.0017551518150502468	2.604818948131109E-4	2.4807799506010558E-5	2.0673166255008797E-5	0.1331889409145197	2.4807799506010558E-5
62-63	0.0018171713138152733	2.74953111191617E-4	2.4807799506010558E-5	2.0673166255008797E-5	0.13527693070627556	3.307706600801408E-5
64-65	0.001839911796695783	3.038955439486293E-4	2.4807799506010558E-5	2.2740482880509676E-5	0.13708790007021435	3.721169925901584E-5
66-67	0.0019143351952138148	3.369726099566434E-4	2.4807799506010558E-5	2.4807799506010558E-5	0.1384523290430449	3.721169925901584E-5
68-69	0.0019515468944728306	3.535111429606504E-4	2.4807799506010558E-5	2.4807799506010558E-5	0.13972579608435348	3.721169925901584E-5
70-71	0.001982556643855344	3.576457762116522E-4	2.4807799506010558E-5	2.4807799506010558E-5	0.14087729144475747	3.721169925901584E-5
72-73	0.0020259702929908622	3.824535757176627E-4	2.4807799506010558E-5	2.4807799506010558E-5	0.14182205514261137	4.9615599012021116E-5
74-75	0.0020425088259948695	3.927901588451671E-4	2.4807799506010558E-5	2.894243275701232E-5	0.14288879052136982	5.375023226302288E-5
76-77	0.002077653208628384	4.217325916021795E-4	2.894243275701232E-5	2.894243275701232E-5	0.14416019024605287	5.788486551402464E-5
78-79	0.0021272688076404054	4.610116074866962E-4	2.894243275701232E-5	2.894243275701232E-5	0.14576442794744154	5.788486551402464E-5
80-81	0.002143807340644412	4.8168477374170495E-4	2.894243275701232E-5	2.894243275701232E-5	0.14717227056940763	5.788486551402464E-5
82-83	0.0021603458736484194	4.961559901202112E-4	2.894243275701232E-5	2.894243275701232E-5	0.14884472971943785	5.788486551402464E-5
84-85	0.0021892883064054316	5.064925732477156E-4	2.894243275701232E-5	2.894243275701232E-5	0.1504158903548185	5.788486551402464E-5
86-87	0.0022140961059114424	5.126945231242182E-4	2.894243275701232E-5	2.894243275701232E-5	0.1522516675182633	5.788486551402464E-5
88-89	0.0022265000056644474	5.188964730007209E-4	3.10097493825132E-5	2.894243275701232E-5	0.15398821348368402	7.028876526702991E-5
90-91	0.0022513078051704582	5.33367689379227E-4	3.307706600801408E-5	2.894243275701232E-5	0.15490196743215542	7.028876526702991E-5
92-93	0.0022575097550469607	5.375023226302288E-4	3.307706600801408E-5	2.894243275701232E-5	0.15590668331214885	7.028876526702991E-5
94-95	0.002274048288050968	5.416369558812305E-4	3.307706600801408E-5	2.894243275701232E-5	0.15676668702835722	7.028876526702991E-5
96-97	0.002274048288050968	5.478389057577332E-4	3.307706600801408E-5	2.894243275701232E-5	0.15764736391082057	7.23560818925308E-5
98-99	0.0022947214543059765	5.602428055107385E-4	3.307706600801408E-5	2.894243275701232E-5	0.15851563689353096	8.062534839453431E-5
100-101	0.0023174619371864862	5.623101221362393E-4	3.307706600801408E-5	2.894243275701232E-5	0.1593094864777233	8.269266502003519E-5
102-103	0.0023195292538119873	5.664447553872411E-4	4.3413649135518476E-5	3.10097493825132E-5	0.1600578550961546	8.682729827103695E-5
104-105	0.0023195292538119873	5.788486551402464E-4	4.548096576101936E-5	3.307706600801408E-5	0.16085170468034696	8.682729827103695E-5
106-107	0.0023340004701904934	5.933198715187525E-4	4.754828238652024E-5	3.307706600801408E-5	0.16156699623277027	8.682729827103695E-5
108-109	0.0023464043699434984	6.222623042757648E-4	5.375023226302287E-5	3.307706600801408E-5	0.16228022046856805	8.682729827103695E-5
110-111	0.0023629429029475056	6.325988874032692E-4	5.788486551402464E-5	3.307706600801408E-5	0.1630968105356409	9.096193152203872E-5
112-113	0.0023753468027005106	6.53272053658278E-4	5.788486551402464E-5	3.307706600801408E-5	0.1637893616051837	9.302924814753959E-5
114-115	0.0023815487525770136	6.594740035347807E-4	5.788486551402464E-5	3.307706600801408E-5	0.16460388435563106	9.923119802404223E-5
116-117	0.0023856833858280154	6.636086367857824E-4	5.788486551402464E-5	3.721169925901584E-5	0.16554451342023396	1.0543314790054487E-4
118-119	0.0023856833858280154	6.69810586662285E-4	5.788486551402464E-5	3.721169925901584E-5	0.16628254545553778	1.0750046452604575E-4
120-121	0.0023856833858280154	6.739452199132868E-4	5.788486551402464E-5	4.1346332510017594E-5	0.16704125065709657	1.0956778115154663E-4
122-123	0.0023856833858280154	6.822144864152903E-4	5.788486551402464E-5	4.1346332510017594E-5	0.1677854846422769	1.1370241440254839E-4
124-125	0.0023877507024535165	6.884164362917929E-4	6.20194987650264E-5	4.1346332510017594E-5	0.16855452642696322	1.1990436427905103E-4
126-127	0.0023980872855810208	6.904837529172938E-4	6.20194987650264E-5	4.1346332510017594E-5	0.16944347257592862	1.2197168090455191E-4
128-129	0.0024187604518360294	6.966857027937965E-4	6.20194987650264E-5	4.1346332510017594E-5	0.17031381287526448	1.240389975300528E-4
130-131	0.0024352989848400366	7.111569191723026E-4	6.20194987650264E-5	4.1346332510017594E-5	0.17120689365748087	1.240389975300528E-4
132-133	0.002458039467720546	7.111569191723026E-4	6.20194987650264E-5	4.548096576101936E-5	0.1719738681255417	1.3024094740655544E-4
134-135	0.0024766453173500538	7.132242357978035E-4	6.20194987650264E-5	4.548096576101936E-5	0.172689159677965	1.3230826403205632E-4
136-137	0.002486981900477558	7.152915524233044E-4	6.20194987650264E-5	4.548096576101936E-5	0.17350368242841235	1.343755806575572E-4
138-139	0.0025014531168560646	7.276954521763096E-4	6.20194987650264E-5	4.548096576101936E-5	0.17424378178034167	1.4884679703606335E-4
>>END_MODULE
