##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24134166
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.68593325329742	37.0	35.0	38.0	33.0	39.0
2	35.277064349354355	35.0	34.0	37.0	33.0	39.0
3	35.47457525567695	35.0	34.0	37.0	33.0	39.0
4	35.51479442049085	36.0	35.0	38.0	33.0	39.0
5	35.419535980650835	36.0	35.0	37.0	33.0	39.0
6	35.38845937332162	35.0	35.0	37.0	33.0	39.0
7	35.40742257263002	35.0	34.0	37.0	33.0	39.0
8	35.520607590086186	36.0	35.0	38.0	33.0	39.0
9	35.46808275869156	36.0	34.0	38.0	33.0	39.0
10-14	35.46930190999764	35.8	34.4	37.8	33.0	39.0
15-19	35.44552053715053	36.0	34.0	38.0	33.0	39.0
20-24	35.42572509860088	36.0	34.0	38.0	33.0	39.0
25-29	35.40437825777779	36.0	34.0	38.0	33.0	39.0
30-34	35.383820903527386	36.0	34.0	38.0	33.0	39.0
35-39	35.35435936754558	36.0	34.0	38.0	33.0	39.0
40-44	35.33353180714842	36.0	34.0	37.8	32.8	39.0
45-49	35.30136602192924	36.0	34.0	38.0	32.0	39.0
50-54	35.27186407021482	36.0	34.0	37.2	32.0	39.0
55-59	35.24886632502652	36.0	34.0	37.2	32.0	39.0
60-64	35.20210628368098	35.6	34.0	37.0	32.0	39.0
65-69	35.17146212551948	35.6	34.0	37.0	32.0	39.0
70-74	35.130535498927124	35.2	34.0	37.0	32.0	39.0
75-79	35.08520716232747	35.0	34.0	37.0	32.0	39.0
80-84	35.04120266679196	35.0	34.0	37.0	32.0	39.0
85-89	34.985925231474745	35.0	34.0	37.0	32.0	39.0
90-94	34.92397139391517	35.0	34.0	37.0	31.0	39.0
95-99	34.85378060298417	35.0	34.0	37.0	31.0	39.0
100-104	34.8007544076725	35.0	34.0	37.0	31.0	39.0
105-109	34.73405493274556	35.0	34.0	37.0	31.0	39.0
110-114	34.65708914076418	35.0	34.0	37.0	30.6	39.0
115-119	34.58203543474425	35.0	34.0	37.0	30.0	39.0
120-124	34.49880946372872	35.0	34.0	37.0	30.0	39.0
125-129	34.412396052964915	35.0	34.0	37.0	30.0	39.0
130-134	34.32081984519374	35.0	34.0	37.0	29.8	39.0
135-139	34.23671043780838	35.0	34.0	37.0	29.0	39.0
140-144	34.09825049682678	35.0	34.0	37.0	29.0	39.0
145-149	34.01209480369034	35.0	34.0	37.0	28.6	39.0
150	33.94194889518867	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	243.0
22	1975.0
23	9770.0
24	31819.0
25	77409.0
26	152076.0
27	262065.0
28	385686.0
29	511064.0
30	661865.0
31	868024.0
32	1166818.0
33	1635442.0
34	2465490.0
35	4204261.0
36	1.0234715E7
37	1465427.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.45273808094301	18.46542366535475	15.272870833821232	31.80896741988101
2	23.853623116705172	25.559043556756837	33.54761875757381	17.039714568964182
3	19.890913156062652	29.011174448704796	29.695113558098505	21.402798837134046
4	23.661008215490025	33.6191604880815	22.379004105631825	20.34082719079665
5	25.089870518003398	34.80249535036761	22.69810359305559	17.409530538573406
6	18.95964832594588	38.636963879340186	23.174436605764626	19.22895118894931
7	20.22572066505219	20.532397929143272	38.09297988585974	21.148901519944797
8	21.545443086784104	23.26634779921544	28.519183136471344	26.66902597752912
9	23.308354637156302	23.062396272570595	29.067592391632672	24.561656698640423
10-14	23.094673335718333	28.92676962609771	25.758709043436596	22.21984799474736
15-19	22.87785954567479	28.13646512583033	27.23326092975411	21.752414398740772
20-24	22.966808962862025	28.2042428978072	27.10192678711168	21.727021352219094
25-29	23.037289956487413	28.165449761139456	27.070053301199636	21.727206981173495
30-34	23.029187749848077	27.949175455244653	27.230495555553897	21.79114123935337
35-39	23.089399484531597	27.957492295362517	27.220256958537536	21.73285126156835
40-44	23.237804032673015	27.741513835613794	27.304444661564027	21.716237470149167
45-49	23.23552593447812	27.57476599771461	27.399440278980432	21.790267788826846
50-54	23.294834385410294	27.59709865259069	27.344801556432486	21.763265405566532
55-59	23.46870592892007	27.487688014474482	27.235476272397204	21.808129784208244
60-64	23.37226652041757	27.461849727891984	27.32331086145674	21.842572890233704
65-69	23.46564368538776	27.389940882978927	27.273612023717746	21.870803407915567
70-74	23.538608490043604	27.364025981788853	27.200096553575513	21.89726897459203
75-79	23.45981294733781	27.404588167662393	27.272802383144295	21.862796501855502
80-84	23.559373243387597	27.46079087597882	27.143708443405156	21.836127437228427
85-89	23.608810016472084	27.377382752733197	27.18751085079965	21.826296379995068
90-94	23.517525515632283	27.333986410253303	27.27374815664853	21.874739917465885
95-99	23.593648108660563	27.43396643579894	27.27357390348604	21.69881155205446
100-104	23.74908749695349	27.399668171669987	27.095235029045543	21.756009302330977
105-109	23.542274466828477	27.51854777165285	27.209267558696666	21.72991020282201
110-114	23.561400050036948	27.49782859701885	27.154033829053798	21.786737523890405
115-119	23.64014484693608	27.49323510909803	27.178713364281986	21.687906679683895
120-124	23.60957905071176	27.400119813545658	27.368969783335377	21.621331352407207
125-129	23.565910425060732	27.51055066524074	27.32220439659372	21.60133451310481
130-134	23.636042778524022	27.402915020970685	27.3832714998314	21.57777070067389
135-139	23.545689542369104	27.396073268079785	27.492754462698233	21.565482726852878
140-144	23.57273272731059	27.535927510699377	27.461385054377974	21.429954707612062
145-149	23.66709087854952	28.008992728400063	27.03056074115012	21.2933556519003
150	23.28520488340057	27.96743836103556	27.1613404830314	21.586016272532476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	0.5
7	0.5
8	2.0
9	5.0
10	6.0
11	5.5
12	12.0
13	14.5
14	18.5
15	35.5
16	59.5
17	107.0
18	187.0
19	321.0
20	524.0
21	836.0
22	1354.0
23	2400.0
24	4053.0
25	6595.5
26	10313.0
27	16480.5
28	27613.0
29	42106.5
30	57058.5
31	75138.5
32	100480.0
33	134008.0
34	180047.5
35	248982.5
36	347956.5
37	470556.0
38	615505.5
39	798068.5
40	1013461.0
41	1221564.5
42	1407095.5
43	1574489.0
44	1696160.0
45	1752270.5
46	1751909.5
47	1698129.5
48	1593393.0
49	1445623.0
50	1259356.0
51	1056607.5
52	862419.0
53	686488.5
54	534140.5
55	403046.0
56	297266.5
57	219179.5
58	159994.0
59	114905.5
60	79131.5
61	52640.0
62	35513.0
63	24102.0
64	16042.5
65	10766.5
66	7684.0
67	5818.5
68	5020.0
69	3700.0
70	1994.5
71	1259.0
72	701.5
73	473.0
74	398.0
75	277.0
76	191.0
77	80.0
78	20.5
79	2.0
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	8.287006893049463E-7
60-64	0.0
65-69	0.0
70-74	8.287006893049463E-7
75-79	0.0
80-84	8.287006893049463E-7
85-89	0.0
90-94	8.287006893049463E-7
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	2.486102067914839E-6
130-134	0.0
135-139	0.0
140-144	8.287006893049463E-7
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4134166E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.19169438951569
#Duplication Level	Percentage of total
1	31.02825300752966
2	10.469127206631388
3	6.975281059742061
4	4.996510997567518
5	3.8605686902942056
6	3.031826277499846
7	2.5757797952231805
8	2.181178646648947
9	1.8552021615251868
>10	20.462494683668893
>50	5.072565096498855
>100	5.786080417299842
>500	0.9471414260864452
>1k	0.6622639032567655
>5k	0.09572663052699408
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.486102067914839E-5	0.0	0.0	6.79534565230056E-4	0.0
2	4.143503446524732E-6	2.9004524125673122E-5	0.0	0.0	0.004744311446270818	0.0
3	2.9004524125673122E-5	3.3148027572197856E-5	0.0	0.0	0.0062401161904662465	0.0
4	7.87265654839699E-5	3.7291531018722584E-5	0.0	0.0	0.007553606783014586	0.0
5	8.701357237701937E-5	4.557853791177205E-5	0.0	0.0	0.008883671389349024	0.0
6	9.944408271659356E-5	4.557853791177205E-5	0.0	0.0	0.010317323581846583	0.0
7	1.1187459305616775E-4	5.3865544804821516E-5	0.0	0.0	0.012248196187927107	0.0
8	1.6159663441446453E-4	5.3865544804821516E-5	0.0	0.0	0.017245261344435932	0.0
9	1.6574013786098928E-4	5.3865544804821516E-5	0.0	4.143503446524732E-6	0.022892856542049145	0.0
10-11	1.9267291026340004E-4	5.3865544804821516E-5	0.0	4.143503446524732E-6	0.028737268153372277	0.0
12-13	2.1960568266581078E-4	6.422430342113334E-5	0.0	4.143503446524732E-6	0.03433928481307372	0.0
14-15	2.5896896540779574E-4	7.251131031418281E-5	0.0	4.143503446524732E-6	0.041797591016818236	0.0
16-17	2.8797348953346884E-4	7.458306203744517E-5	0.0	4.143503446524732E-6	0.04722972403521215	0.0
18-19	3.273367722754538E-4	7.665481376070753E-5	0.0	4.143503446524732E-6	0.05456579688728419	0.0
20-21	3.6462830329417643E-4	8.908532410028173E-5	0.0	4.143503446524732E-6	0.0602133920848974	4.143503446524732E-6
22-23	4.2056559982226024E-4	1.0980284133290539E-4	4.143503446524732E-6	4.143503446524732E-6	0.06592728333765502	4.143503446524732E-6
24-25	4.847899032433936E-4	1.1394634477943013E-4	4.143503446524732E-6	4.143503446524732E-6	0.07072338857700738	4.143503446524732E-6
26-27	5.137944273690667E-4	1.2016159994921722E-4	4.143503446524732E-6	4.143503446524732E-6	0.07425572526516971	4.143503446524732E-6
28-29	6.153102618089226E-4	1.2016159994921722E-4	4.143503446524732E-6	4.143503446524732E-6	0.07813404449111687	4.143503446524732E-6
30-31	8.079831720723227E-4	1.3259211028879143E-4	8.287006893049464E-6	4.143503446524732E-6	0.08272297455814301	4.143503446524732E-6
32-33	9.654363030402624E-4	1.4502262062836562E-4	8.287006893049464E-6	4.143503446524732E-6	0.08715030799075468	6.2152551697870985E-6
34-35	0.0011001001650523161	1.5330962752141509E-4	8.287006893049464E-6	4.143503446524732E-6	0.09017506550671775	8.287006893049464E-6
36-37	0.001139463447794301	1.574531309679398E-4	8.287006893049464E-6	4.143503446524732E-6	0.09328476484333456	8.287006893049464E-6
38-39	0.0011871137374293355	1.574531309679398E-4	8.287006893049464E-6	4.143503446524732E-6	0.09635717264893265	8.287006893049464E-6
40-41	0.0012181900132782711	1.6159663441446455E-4	8.287006893049464E-6	6.2152551697870985E-6	0.09913124820638095	8.287006893049464E-6
42-43	0.0012430510339574196	1.760988964773011E-4	8.287006893049464E-6	8.287006893049464E-6	0.10189082150176643	8.287006893049464E-6
44-45	0.0012554815442969937	2.071751723262366E-4	8.287006893049464E-6	8.287006893049464E-6	0.10409723708704083	1.2430510339574195E-5
46-47	0.001309347089101815	2.237491861123355E-4	8.287006893049464E-6	8.287006893049464E-6	0.10647146456189951	1.2430510339574195E-5
48-49	0.0013507821235670624	2.2996444128212261E-4	8.287006893049464E-6	8.287006893049464E-6	0.10883533327814186	1.2430510339574195E-5
50-51	0.0013942889097555721	2.444667033449592E-4	8.287006893049464E-6	8.287006893049464E-6	0.11086150646349247	1.2430510339574195E-5
52-53	0.0014481544545603938	2.506819585147463E-4	8.287006893049464E-6	8.287006893049464E-6	0.11297054971777355	1.8645765509361292E-5
54-55	0.0015082352545350024	2.672559723008452E-4	8.287006893049464E-6	8.287006893049464E-6	0.11495321611693562	2.071751723262366E-5
56-57	0.0015641725510630862	2.817582343636818E-4	8.287006893049464E-6	8.287006893049464E-6	0.11704982886087714	2.071751723262366E-5
58-59	0.0016035358338050711	2.9418874470325595E-4	8.287006893049464E-6	8.287006893049464E-6	0.11895791219800178	2.071751723262366E-5
60-61	0.0016781188958425162	3.1904976538240433E-4	8.287006893049464E-6	8.287006893049464E-6	0.12044542993530416	2.071751723262366E-5
62-63	0.0017195539303077637	3.3355202744524094E-4	8.287006893049464E-6	8.287006893049464E-6	0.12245710085859193	2.486102067914839E-5
64-65	0.0017444149509869122	3.376955308917656E-4	8.287006893049464E-6	1.2430510339574197E-5	0.12437554295433287	2.6932772402410755E-5
66-67	0.0018169262613010949	3.5841304812438927E-4	8.287006893049464E-6	1.6574013786098928E-5	0.1256579572710323	2.9004524125673122E-5
68-69	0.0018459307854267679	3.770588136337506E-4	8.287006893049464E-6	1.6574013786098928E-5	0.1269610891049643	3.107627584893549E-5
70-71	0.0018604330474896046	3.812023170802753E-4	8.287006893049464E-6	1.6574013786098928E-5	0.12829115371129873	3.5219779295460224E-5
72-73	0.0018894375716152776	3.9363282741984953E-4	8.287006893049464E-6	1.6574013786098928E-5	0.1292731640281251	3.7291531018722584E-5
74-75	0.0019163703440176884	3.998480825896366E-4	8.287006893049464E-6	1.6574013786098928E-5	0.13039190995868677	3.7291531018722584E-5
76-77	0.0019453748681433616	4.081350894826861E-4	1.2430510339574195E-5	1.6574013786098928E-5	0.131688826537449	3.7291531018722584E-5
78-79	0.001972307640545772	4.3713961360835924E-4	1.2430510339574195E-5	1.6574013786098928E-5	0.13328407536436104	3.7291531018722584E-5
80-81	0.001982666399162084	4.495701239479334E-4	1.8645765509361292E-5	2.2789268955886027E-5	0.13470322529479578	3.7291531018722584E-5
82-83	0.001993025157778396	4.6614413773403234E-4	2.486102067914839E-5	2.486102067914839E-5	0.13633783740444977	3.7291531018722584E-5
84-85	0.0020199579301808067	4.6821588945729467E-4	2.9004524125673122E-5	2.9004524125673122E-5	0.13798695177616663	3.7291531018722584E-5
86-87	0.0020468907025832173	4.7235939290381945E-4	2.9004524125673122E-5	3.3148027572197856E-5	0.13979559103057465	3.7291531018722584E-5
88-89	0.0020655364680925788	4.7650289635034417E-4	2.9004524125673122E-5	3.3148027572197856E-5	0.14162494780221532	3.7291531018722584E-5
90-91	0.0020738234749856283	4.806463997968689E-4	2.9004524125673122E-5	3.5219779295460224E-5	0.1426794694293559	3.7291531018722584E-5
92-93	0.0020800387301554155	4.86861654966656E-4	3.107627584893549E-5	3.7291531018722584E-5	0.1436241882151635	3.7291531018722584E-5
94-95	0.0020862539853252022	4.93076910136443E-4	3.3148027572197856E-5	3.7291531018722584E-5	0.14465177706990165	4.143503446524732E-5
96-97	0.002096612743941514	4.992921653062302E-4	3.5219779295460224E-5	3.7291531018722584E-5	0.14559856760743256	4.557853791177205E-5
98-99	0.0021048997508345637	5.179379308155915E-4	4.557853791177205E-5	3.936328274198495E-5	0.1465764344208124	4.557853791177205E-5
100-101	0.002111115006004351	5.220814342621162E-4	4.557853791177205E-5	4.143503446524732E-5	0.14750250744111065	4.557853791177205E-5
102-103	0.002117330261174138	5.282966894319033E-4	4.557853791177205E-5	4.143503446524732E-5	0.14838300192349718	4.972204135829678E-5
104-105	0.002121473764620663	5.345119446016904E-4	4.557853791177205E-5	4.143503446524732E-5	0.1494271647920214	5.3865544804821516E-5
106-107	0.0021339042749602367	5.427989514947399E-4	4.557853791177205E-5	4.143503446524732E-5	0.1502351479640937	5.3865544804821516E-5
108-109	0.0021339042749602367	5.697317238971505E-4	4.557853791177205E-5	4.143503446524732E-5	0.15093747179827965	5.8009048251346244E-5
110-111	0.0021380477784067615	5.904492411297743E-4	4.7650289635034414E-5	4.143503446524732E-5	0.15179724876343353	5.8009048251346244E-5
112-113	0.0021608370473626477	6.090950066391356E-4	5.3865544804821516E-5	4.143503446524732E-5	0.15253479237691495	6.215255169787098E-5
114-115	0.0021649805508091725	6.215255169787098E-4	5.3865544804821516E-5	4.143503446524732E-5	0.15341943036274797	6.629605514439571E-5
116-117	0.002167052302532435	6.256690204252345E-4	5.8009048251346244E-5	4.143503446524732E-5	0.1542833508313484	6.629605514439571E-5
118-119	0.002169124054255697	6.298125238717592E-4	6.215255169787098E-5	4.143503446524732E-5	0.15507890349308112	7.043955859092043E-5
120-121	0.002179482812872009	6.380995307648087E-4	6.215255169787098E-5	4.143503446524732E-5	0.15589724542376976	7.043955859092043E-5
122-123	0.0021836263163185336	6.380995307648087E-4	6.215255169787098E-5	4.143503446524732E-5	0.15665343480276053	7.458306203744517E-5
124-125	0.002191913323211583	6.380995307648087E-4	6.215255169787098E-5	4.143503446524732E-5	0.15754221629204007	7.458306203744517E-5
126-127	0.0021981285783813703	6.401712824880711E-4	6.422430342113334E-5	4.350678618850968E-5	0.15853665711920603	7.87265654839699E-5
128-129	0.002214702592167469	6.505300411043829E-4	6.629605514439571E-5	4.557853791177205E-5	0.15948759116018346	8.079831720723228E-5
130-131	0.002231276605953568	6.608887997206947E-4	6.629605514439571E-5	4.7650289635034414E-5	0.1604820319873494	8.701357237701937E-5
132-133	0.0022437071162931424	6.671040548904818E-4	6.629605514439571E-5	4.972204135829678E-5	0.16133559369733347	8.908532410028173E-5
134-135	0.002266496385249028	6.712475583370065E-4	6.629605514439571E-5	5.179379308155915E-5	0.16222023168316652	9.530057927006883E-5
136-137	0.0022789268955886025	6.712475583370065E-4	6.629605514439571E-5	5.3865544804821516E-5	0.16325817929652098	9.530057927006883E-5
138-139	0.0022892856542049145	6.774628135067936E-4	6.629605514439571E-5	5.3865544804821516E-5	0.16410552575133525	1.0151583443985594E-4
>>END_MODULE
