##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22936848
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.61967320008399	37.0	35.0	38.0	32.0	39.0
2	35.25075346010925	35.0	34.0	37.0	33.0	39.0
3	35.40294804238141	35.0	34.0	37.0	33.0	39.0
4	35.4543439883283	36.0	35.0	38.0	33.0	39.0
5	35.36665312513733	36.0	35.0	37.0	33.0	39.0
6	35.32642431950545	35.0	34.0	37.0	33.0	39.0
7	35.365343921710604	35.0	34.0	37.0	33.0	39.0
8	35.43398665762619	36.0	34.0	38.0	33.0	39.0
9	35.43555117076244	36.0	34.0	38.0	33.0	39.0
10-14	35.42331079667093	35.8	34.4	37.8	33.0	39.0
15-19	35.39547799244255	36.0	34.0	38.0	33.0	39.0
20-24	35.37971592260628	36.0	34.0	38.0	33.0	39.0
25-29	35.356283208573394	36.0	34.0	38.0	33.0	39.0
30-34	35.33773271724171	36.0	34.0	38.0	32.8	39.0
35-39	35.306422931346106	36.0	34.0	37.8	32.0	39.0
40-44	35.28870868394821	35.8	34.0	37.8	32.0	39.0
45-49	35.25431829168507	36.0	34.0	37.4	32.0	39.0
50-54	35.23086261024183	35.8	34.0	37.2	32.0	39.0
55-59	35.19991798349974	35.6	34.0	37.2	32.0	39.0
60-64	35.15636785839101	35.0	34.0	37.0	32.0	39.0
65-69	35.124534312648365	35.0	34.0	37.0	32.0	39.0
70-74	35.09106072464708	35.0	34.0	37.0	32.0	39.0
75-79	35.0394183106589	35.0	34.0	37.0	32.0	39.0
80-84	34.995019402840356	35.0	34.0	37.0	32.0	39.0
85-89	34.94223807909439	35.0	34.0	37.0	31.2	39.0
90-94	34.88163445997462	35.0	34.0	37.0	31.0	39.0
95-99	34.81260665807263	35.0	34.0	37.0	31.0	39.0
100-104	34.75815684875271	35.0	34.0	37.0	31.0	39.0
105-109	34.692280412722795	35.0	34.0	37.0	30.8	39.0
110-114	34.612195747209896	35.0	34.0	37.0	30.0	39.0
115-119	34.54605794135271	35.0	34.0	37.0	30.0	39.0
120-124	34.46587307898627	35.0	34.0	37.0	30.0	39.0
125-129	34.3826899929755	35.0	34.0	37.0	30.0	39.0
130-134	34.275458240818445	35.0	34.0	37.0	29.4	39.0
135-139	34.209332799345404	35.0	34.0	37.0	29.0	39.0
140-144	34.07338986594845	35.0	34.0	37.0	29.0	39.0
145-149	33.99218072160569	35.0	34.0	37.0	28.4	39.0
150	33.91791243504775	35.0	33.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	11.0
21	243.0
22	1956.0
23	9765.0
24	31999.0
25	77275.0
26	153235.0
27	264133.0
28	385410.0
29	506564.0
30	650292.0
31	845833.0
32	1128431.0
33	1571248.0
34	2352976.0
35	3989979.0
36	9549274.0
37	1418217.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.98140407086449	18.42905354737495	15.921677642891474	31.66786473886909
2	24.39167753128067	25.34938104834631	33.066923580781456	17.19201783959156
3	19.8813934678383	29.1764108128545	29.970129287162734	20.97206643214447
4	23.527631172338936	33.791949094313225	22.289270086282126	20.391149647065717
5	25.240704389722595	35.04920118056326	22.414322142257735	17.29577228745641
6	18.886064903076484	38.9352146380357	22.77328602430465	19.405434434583167
7	20.33013864851875	20.522758837657207	38.094183647203835	21.05291886662021
8	21.522535641482968	23.155268285686375	28.429669140989432	26.892526931841225
9	23.466999476126798	22.967484459939744	28.88944897747066	24.676067086462798
10-14	23.14739932880054	29.00161172973723	25.679444708357487	22.17154423310474
15-19	22.96170947289706	28.15582681630885	27.141020422684058	21.741443288110034
20-24	23.04902835821208	28.260332021208846	26.994423994090205	21.696215626488872
25-29	23.146645083927837	28.211939147000493	26.940810699011475	21.700605070060195
30-34	23.12716551114608	27.99455007941806	27.126029696844135	21.75225471259172
35-39	23.209894402229985	27.99482736250421	27.086638059422985	21.70864017584282
40-44	23.365533921661775	27.765844722866888	27.17996910473488	21.688652250736457
45-49	23.35764530505674	27.606157567944823	27.268122455186518	21.768074671811924
50-54	23.43609985120885	27.60368556307301	27.214311225326167	21.745903360391978
55-59	23.611973188295096	27.485469668718217	27.120298307770973	21.78225883521572
60-64	23.516486659370113	27.460513319005297	27.193767862088116	21.82923215953648
65-69	23.59763556003859	27.40203536248747	27.14492069703736	21.85540838043658
70-74	23.6963840890431	27.3441799849744	27.058004656960712	21.901431269021796
75-79	23.6165283041506	27.403284880293928	27.13785346617809	21.842333349377384
80-84	23.714967287571508	27.450271284005545	26.989184390113234	21.84557703830971
85-89	23.75070454318745	27.394545231323853	27.037348810961298	21.817401414527403
90-94	23.68074811325427	27.331034325204577	27.11027251870004	21.87794504284111
95-99	23.756489121783428	27.434801852460282	27.109005561705775	21.69970346405051
100-104	23.924294218630216	27.394553950917754	26.931330756518946	21.749821073933088
105-109	23.70190707982195	27.526404674260384	27.037841468016875	21.73384677790078
110-114	23.72351793661994	27.51539587993326	26.978730311473598	21.78235587197321
115-119	23.808300948761573	27.493233595130423	27.009379841554516	21.68908561455349
120-124	23.773894303175396	27.413620214948452	27.180957034724212	21.631528447151936
125-129	23.75263614035055	27.508428599330024	27.131193676927133	21.607741583392293
130-134	23.817652237735764	27.416667511413372	27.18441160204959	21.58126864880127
135-139	23.72965369958418	27.424199698232293	27.279648886368346	21.56649771581518
140-144	23.7617740676487	27.55418966023579	27.24303705548382	21.440999216631685
145-149	23.85630841691936	28.045288524386613	26.788009407395467	21.310393651298558
150	23.462011868413654	28.0179953235074	26.92785861422633	21.592134193852615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	4.0
10	4.0
11	3.5
12	8.0
13	15.0
14	26.0
15	42.5
16	67.0
17	112.0
18	173.0
19	295.0
20	512.0
21	830.5
22	1360.5
23	2317.0
24	3779.5
25	6139.5
26	9706.5
27	15504.0
28	25629.0
29	38967.5
30	52598.5
31	69108.0
32	92224.5
33	123236.5
34	165528.0
35	228627.0
36	320489.5
37	433524.5
38	568594.5
39	742193.0
40	946250.0
41	1141505.5
42	1318206.5
43	1480866.5
44	1601928.0
45	1661571.5
46	1667335.5
47	1622899.5
48	1528170.0
49	1389943.0
50	1213290.0
51	1021021.0
52	834157.5
53	665302.0
54	521028.0
55	394700.0
56	292101.0
57	215867.5
58	157711.0
59	113089.5
60	78337.0
61	52816.0
62	35951.5
63	24733.5
64	16666.5
65	11186.5
66	7915.5
67	5756.0
68	4819.0
69	3673.5
70	2181.0
71	1472.0
72	846.0
73	636.0
74	554.5
75	367.0
76	247.0
77	93.5
78	24.0
79	2.5
80	1.0
81	0.0
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.11599675770620271
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.018819499523212605
115-119	0.0
120-124	0.0
125-129	8.719593904097024E-7
130-134	0.04727589422923324
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2936848E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.31516778544225
#Duplication Level	Percentage of total
1	30.038273056518822
2	10.596505912474102
3	7.0986171061248395
4	5.050777276944974
5	3.784977203883475
6	3.0269905476480896
7	2.508557643947766
8	2.1047211467889384
9	1.7941844117650978
>10	20.99727997150868
>50	5.230285370766968
>100	6.015904156455379
>500	0.9225276797415458
>1k	0.7331702244656804
>5k	0.09722829096577705
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.615878171229107E-5	4.359796952048511E-6	0.0	5.972921824306461E-4	0.0
2	2.179898476024256E-5	4.359796952048512E-5	4.359796952048511E-6	4.359796952048511E-6	0.004503670251466112	0.0
3	4.795776647253363E-5	4.359796952048512E-5	4.359796952048511E-6	4.359796952048511E-6	0.00612115492067611	0.0
4	1.2643411160940683E-4	4.359796952048512E-5	4.359796952048511E-6	4.359796952048511E-6	0.007503210554475489	0.0
5	1.2643411160940683E-4	4.359796952048512E-5	4.359796952048511E-6	4.359796952048511E-6	0.008972462127315837	0.0
6	1.4387329941760088E-4	4.359796952048512E-5	4.359796952048511E-6	4.359796952048511E-6	0.010646624156902465	0.0
7	1.8311147198603748E-4	6.103715732867916E-5	4.359796952048511E-6	4.359796952048511E-6	0.012617252379228392	0.0
8	2.964661927392988E-4	6.539695428072768E-5	4.359796952048511E-6	4.359796952048511E-6	0.0177138550161731	0.0
9	3.0082598969134733E-4	7.41165481848247E-5	4.359796952048511E-6	8.719593904097023E-6	0.023808851155136923	0.0
10-11	3.2916466987966265E-4	8.501604056494597E-5	4.359796952048511E-6	8.719593904097023E-6	0.029731635314494825	0.0
12-13	3.66222943972075E-4	8.93758375169945E-5	4.359796952048511E-6	8.719593904097023E-6	0.035551964245479586	0.0
14-15	4.098209134925601E-4	9.3735634469043E-5	4.359796952048511E-6	1.089949238012128E-5	0.04361758860676933	0.0
16-17	4.468791875849725E-4	9.591553294506726E-5	4.359796952048511E-6	1.3079390856145534E-5	0.04910657296939841	0.0
18-19	5.144560403417244E-4	9.809543142109151E-5	4.359796952048511E-6	1.3079390856145534E-5	0.05643103184883991	0.0
20-21	5.733132991943793E-4	1.0681502532518853E-4	4.359796952048511E-6	1.3079390856145534E-5	0.06219904321640009	0.0
22-23	6.212710656669129E-4	1.286140100854311E-4	4.359796952048511E-6	1.3079390856145534E-5	0.06819158412699078	0.0
24-25	6.844881214716163E-4	1.329738070374796E-4	4.359796952048511E-6	1.9619086284218302E-5	0.07322714960660681	0.0
26-27	7.564247711804167E-4	1.3515370551350387E-4	4.359796952048511E-6	2.179898476024256E-5	0.07702889254879311	0.0
28-29	9.177372584062116E-4	1.4605319789362514E-4	4.359796952048511E-6	2.179898476024256E-5	0.08087205356202387	0.0
30-31	0.0012120235526694861	1.5477279179772216E-4	8.719593904097023E-6	2.179898476024256E-5	0.08576374574222229	0.0
32-33	0.0014256536033198633	1.722119796059162E-4	8.719593904097023E-6	2.179898476024256E-5	0.09071429518127339	0.0
34-35	0.0015935057859737308	1.78751675033989E-4	8.719593904097023E-6	2.179898476024256E-5	0.093735634469043	6.539695428072767E-6
36-37	0.001661082638730483	1.87471268938086E-4	8.719593904097023E-6	2.179898476024256E-5	0.09687032847756588	8.719593904097023E-6
38-39	0.0017177599991071136	1.87471268938086E-4	8.719593904097023E-6	2.179898476024256E-5	0.10019249375502684	8.719593904097023E-6
40-41	0.0017591780701515744	1.87471268938086E-4	8.719593904097023E-6	2.179898476024256E-5	0.10291300705310512	8.719593904097023E-6
42-43	0.00178751675033989	1.9837076131820727E-4	8.719593904097023E-6	2.179898476024256E-5	0.10550272644262193	8.719593904097023E-6
44-45	0.001798416242720011	2.2452954303049834E-4	8.719593904097023E-6	2.179898476024256E-5	0.10768262491864619	8.719593904097023E-6
46-47	0.001839834313764472	2.441486293147166E-4	8.719593904097023E-6	2.179898476024256E-5	0.11016334938436179	1.3079390856145534E-5
48-49	0.001909591064997248	2.5286822321881366E-4	8.719593904097023E-6	2.179898476024256E-5	0.11243262369790305	1.3079390856145534E-5
50-51	0.0019488292375656846	2.5286822321881366E-4	8.719593904097023E-6	2.179898476024256E-5	0.1144337704988933	1.3079390856145534E-5
52-53	0.0020164060903224367	2.5940791864688644E-4	8.719593904097023E-6	2.179898476024256E-5	0.11648941476178418	1.7439187808194046E-5
54-55	0.0020665437552709947	2.768471064550805E-4	8.719593904097023E-6	2.179898476024256E-5	0.11819627526851118	2.179898476024256E-5
56-57	0.0021406603034558195	2.8338680188315325E-4	8.719593904097023E-6	2.179898476024256E-5	0.1200317497853236	2.179898476024256E-5
58-59	0.0022016974607844985	2.921063957872503E-4	1.089949238012128E-5	2.179898476024256E-5	0.12179964744937927	2.179898476024256E-5
60-61	0.002258374821161129	3.0518578664339585E-4	1.3079390856145534E-5	2.179898476024256E-5	0.12319260257555877	2.3978883236266812E-5
62-63	0.002291073298301493	3.1826517749954135E-4	1.3079390856145534E-5	2.179898476024256E-5	0.12505423587408349	2.615878171229107E-5
64-65	0.0023063325876336626	3.400641622597839E-4	1.3079390856145534E-5	2.179898476024256E-5	0.12681341394423506	2.615878171229107E-5
66-67	0.0023695496434383663	3.705827409241235E-4	1.3079390856145534E-5	2.179898476024256E-5	0.1280951942481373	2.615878171229107E-5
68-69	0.002393528526674633	3.74942537876172E-4	1.3079390856145534E-5	2.179898476024256E-5	0.12928105901909454	2.615878171229107E-5
70-71	0.0024044280190547544	3.74942537876172E-4	1.3079390856145534E-5	2.179898476024256E-5	0.13048436297785992	2.615878171229107E-5
72-73	0.002441486293147167	3.9238172568436603E-4	1.3079390856145534E-5	2.179898476024256E-5	0.1313737615560778	3.051857866433958E-5
74-75	0.002454565684003312	3.989214211124388E-4	1.3079390856145534E-5	2.179898476024256E-5	0.13237433495657291	3.051857866433958E-5
76-77	0.002465465176383433	4.2072040587268135E-4	1.7439187808194046E-5	2.179898476024256E-5	0.13354930023515	3.051857866433958E-5
78-79	0.002498163653523797	4.3815959368087543E-4	2.179898476024256E-5	2.179898476024256E-5	0.13498149353389793	3.051857866433958E-5
80-81	0.002515602841331991	4.59958578441118E-4	2.179898476024256E-5	2.3978883236266812E-5	0.13623493515761187	3.051857866433958E-5
82-83	0.0025265023337121125	4.77397766249312E-4	2.3978883236266812E-5	2.615878171229107E-5	0.1378349806390137	3.051857866433958E-5
84-85	0.002548301318472355	4.8611736015340904E-4	3.051857866433958E-5	2.615878171229107E-5	0.13936526936918273	3.051857866433958E-5
86-87	0.002574460100184646	4.970168525335303E-4	3.051857866433958E-5	2.615878171229107E-5	0.14109174896219395	3.051857866433958E-5
88-89	0.0026006188818969373	4.970168525335303E-4	3.051857866433958E-5	2.615878171229107E-5	0.14289234510338997	3.487837561638809E-5
90-91	0.002624597765133204	5.166359388177487E-4	3.051857866433958E-5	2.615878171229107E-5	0.1438188019557003	3.9238172568436604E-5
92-93	0.002639857054465374	5.188158372937729E-4	3.051857866433958E-5	2.615878171229107E-5	0.14474089901105852	3.9238172568436604E-5
94-95	0.0026463967498934465	5.253555327218457E-4	3.051857866433958E-5	2.615878171229107E-5	0.14560413880756415	3.9238172568436604E-5
96-97	0.0026551163437975437	5.36255025101967E-4	3.051857866433958E-5	2.615878171229107E-5	0.1464455796193095	5.0137664948557884E-5
98-99	0.0026616560392256163	5.44974619006064E-4	3.051857866433958E-5	2.615878171229107E-5	0.1472717611417227	5.667736037663065E-5
100-101	0.0026660158361776647	5.44974619006064E-4	3.051857866433958E-5	2.615878171229107E-5	0.14804562510071131	5.667736037663065E-5
102-103	0.0026703756331297136	5.44974619006064E-4	3.051857866433958E-5	2.615878171229107E-5	0.14880422977036775	5.667736037663065E-5
104-105	0.0026790952270338104	5.536942129101609E-4	3.051857866433958E-5	2.615878171229107E-5	0.1496260514958289	5.667736037663065E-5
106-107	0.002700894211794053	5.580540098622095E-4	3.051857866433958E-5	2.615878171229107E-5	0.15040427525176955	5.667736037663065E-5
108-109	0.0027074339072221255	5.733132991943793E-4	3.487837561638809E-5	2.615878171229107E-5	0.15123917636808684	5.667736037663065E-5
110-111	0.0027117937041741744	5.820328930984764E-4	3.487837561638809E-5	2.615878171229107E-5	0.15216999301734918	5.88572588526549E-5
112-113	0.0027161535011262228	5.951122839546218E-4	3.487837561638809E-5	2.615878171229107E-5	0.15294603687481384	6.103715732867916E-5
114-115	0.0027248730950303196	6.038318778587189E-4	3.487837561638809E-5	2.615878171229107E-5	0.15388339321950428	6.103715732867916E-5
116-117	0.0027314127904583927	6.060117763347431E-4	3.9238172568436604E-5	2.615878171229107E-5	0.15485126814285904	6.103715732867916E-5
118-119	0.0027401323843624895	6.147313702388401E-4	4.795776647253363E-5	2.615878171229107E-5	0.15562731200032368	6.975675123277618E-5
120-121	0.0027488519782665867	6.147313702388401E-4	4.795776647253363E-5	2.615878171229107E-5	0.15639899606083627	7.41165481848247E-5
122-123	0.002753211775218635	6.147313702388401E-4	4.795776647253363E-5	3.051857866433958E-5	0.15710746306554413	7.629644666084896E-5
124-125	0.0027597514706467078	6.147313702388401E-4	4.795776647253363E-5	3.051857866433958E-5	0.1579140255016731	7.847634513687321E-5
126-127	0.0027706509630268292	6.234509641429371E-4	4.795776647253363E-5	3.051857866433958E-5	0.15881868336922317	8.283614208892171E-5
128-129	0.0027815504554069507	6.256308626189614E-4	4.795776647253363E-5	3.051857866433958E-5	0.15977129900324577	8.719593904097023E-5
130-131	0.0028011695416911685	6.408901519511312E-4	5.2317563424582144E-5	3.051857866433958E-5	0.1607173749418403	8.719593904097023E-5
132-133	0.0028360479173075567	6.496097458552283E-4	6.103715732867916E-5	3.051857866433958E-5	0.1614193022511201	8.719593904097023E-5
134-135	0.0028643865974958722	6.583293397593253E-4	6.103715732867916E-5	3.051857866433958E-5	0.16225638326591343	8.93758375169945E-5
136-137	0.002873106191399969	6.692288321394466E-4	6.321705580470342E-5	3.051857866433958E-5	0.16304986631118626	1.0681502532518853E-4
138-139	0.002888365480732139	6.801283245195678E-4	6.539695428072768E-5	3.269847714036383E-5	0.16388694732597958	1.133547207532613E-4
>>END_MODULE
