##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24133957
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.714761363004	37.0	35.0	38.0	33.0	39.0
2	35.32576477201812	35.0	34.0	37.0	33.0	39.0
3	35.46118699059586	35.0	34.0	37.0	33.0	39.0
4	35.54069309893939	36.0	35.0	38.0	33.0	39.0
5	35.464769701876904	36.0	35.0	38.0	33.0	39.0
6	35.41336748051718	35.0	35.0	37.0	33.0	39.0
7	35.45037015687067	35.0	34.0	38.0	33.0	39.0
8	35.57828212754336	36.0	35.0	38.0	33.0	39.0
9	35.57474060304325	36.0	35.0	38.0	33.0	39.0
10-14	35.526052980039694	35.8	34.8	37.8	33.0	39.0
15-19	35.502075113500865	36.0	35.0	38.0	33.0	39.0
20-24	35.47190245677491	36.0	34.8	38.0	33.0	39.0
25-29	35.4702349473814	36.0	34.2	38.0	33.0	39.0
30-34	35.455575693617085	36.0	34.0	38.0	33.0	39.0
35-39	35.433638362743416	36.0	34.0	38.0	33.0	39.0
40-44	35.41571757171856	36.0	34.0	38.0	33.0	39.0
45-49	35.391904700915816	36.0	34.0	38.0	33.0	39.0
50-54	35.37385597397061	36.0	34.0	38.0	32.4	39.0
55-59	35.34725010076052	36.0	34.0	38.0	32.0	39.0
60-64	35.30974395123021	36.0	34.0	38.0	32.0	39.0
65-69	35.27745512267218	36.0	34.0	38.0	32.0	39.0
70-74	35.26236513970751	36.0	34.0	38.0	32.0	39.0
75-79	35.21067938423856	36.0	34.0	38.0	32.0	39.0
80-84	35.13680003656259	36.0	34.0	38.0	32.0	39.0
85-89	35.12789378053504	36.0	34.0	38.0	32.0	39.0
90-94	35.086426937779	36.0	34.0	38.0	32.0	39.0
95-99	35.022906910789644	35.2	34.0	38.0	32.0	39.0
100-104	34.96300027384651	35.2	34.0	38.0	31.0	39.0
105-109	34.9148119970546	35.0	34.0	37.6	31.0	39.0
110-114	34.84622454577175	35.0	34.0	37.2	31.0	39.0
115-119	34.788239682369536	35.0	34.0	37.0	31.0	39.0
120-124	34.72194335972339	35.0	34.0	37.0	30.6	39.0
125-129	34.64614000099528	35.0	34.0	37.0	30.0	39.0
130-134	34.573564011902405	35.0	34.0	37.0	30.0	39.0
135-139	34.49454084135478	35.0	34.0	37.0	30.0	39.0
140-144	34.40397779775609	35.0	34.0	37.0	30.0	39.0
145-149	34.30210319841044	35.0	34.0	37.0	29.0	39.0
150	34.242408445494455	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	16.0
21	253.0
22	2019.0
23	9626.0
24	30668.0
25	73512.0
26	144044.0
27	249304.0
28	367025.0
29	483964.0
30	621972.0
31	809056.0
32	1080973.0
33	1506589.0
34	2253758.0
35	3833125.0
36	1.0377171E7
37	2290874.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.538268653510436	18.58414343674116	15.369283013526669	32.50830489622174
2	24.271329573982815	25.500904203189894	32.75034561262977	17.477420610197516
3	19.915026781559277	29.230158983046174	29.952353026898987	20.902461208495566
4	23.43234886844292	34.20732455933356	21.53203886126092	20.828287710962606
5	25.45349276954459	34.999677011109284	21.61385304531702	17.93297717402911
6	19.117143496905356	39.083952916794914	21.773765560855416	20.025138025444317
7	20.37338510216124	20.498337674174195	37.089549799065274	22.038727424599287
8	21.571365193034858	23.3044833882815	28.056899247810875	27.06725217087277
9	23.57776638120305	23.426858678831657	27.50385276645682	25.491522173508475
10-14	23.118911664589444	28.832586384404348	25.14312427091836	22.905377680087852
15-19	23.18123629705647	27.646775039832878	26.69346431668872	22.47852434642193
20-24	23.084034581750327	27.614317653719926	26.64583963703918	22.655808127490566
25-29	23.170937115699676	27.220476111729212	26.63544316416906	22.97314360840205
30-34	22.98690780784028	26.947888628026384	26.854929979819968	23.210273584313367
35-39	23.29153565658545	26.68376594853467	26.873706620095493	23.150991774784384
40-44	23.45453254930387	26.33068667521037	26.96016322561609	23.25461754986967
45-49	23.367419607153522	26.164718036085006	27.055489491424883	23.412372865336586
50-54	23.565647309826478	26.189025734620337	26.771396891819997	23.47393006373319
55-59	23.63306191355193	26.190814875488506	26.799474284304058	23.3766489266555
60-64	23.333106129260113	26.191812639758993	26.850535119458446	23.62454611152245
65-69	23.548818348334976	26.16208732222548	26.791812495428125	23.497281834011417
70-74	23.71989143761216	26.13184982470964	26.510548601706716	23.63771013597149
75-79	23.55725751893898	26.25005671469457	26.767137274670706	23.425548491695746
80-84	23.858996578528817	26.23351167662039	26.45636587683699	23.451125868013804
85-89	23.612577233504528	26.301813096866507	26.655189062249057	23.430420607379908
90-94	23.644039173882998	26.10849622470527	26.648730886930238	23.598733714481497
95-99	23.644357201763473	26.32240125396759	26.689896729326236	23.343344814942697
100-104	23.887147060053184	26.078596228542217	26.647475173673342	23.386781537731256
105-109	23.656809366155745	26.300813414062183	26.56455134978487	23.477825869997197
110-114	23.77063736377752	26.3823068881742	26.505074157544904	23.341981590503373
115-119	23.87658849313438	26.433573242879316	26.70040474506522	22.98943351892108
120-124	23.834021084897103	26.55260966943796	26.828759991575357	22.78460925408958
125-129	23.809671161674814	26.617968201401865	26.673351576784526	22.89900906013879
130-134	23.99264654362316	26.612972750386522	26.644073327884026	22.750307378106292
135-139	23.754642473258734	26.640061553105443	26.90995015860847	22.69534581502735
140-144	23.78080395187577	26.817383489992956	26.6968404725342	22.70497208559707
145-149	23.922502551614233	27.37513338360295	26.20349977352144	22.498864291261373
150	23.734607631893933	27.391799032375836	26.12513563358052	22.748457702149715
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	12.0
3	28.0
4	39.0
5	38.5
6	25.0
7	15.0
8	15.5
9	13.0
10	7.5
11	6.5
12	9.0
13	12.5
14	15.5
15	26.5
16	50.0
17	94.5
18	158.0
19	226.5
20	382.0
21	613.0
22	982.0
23	1625.0
24	2584.0
25	4202.5
26	6531.0
27	10075.0
28	15324.0
29	22084.0
30	31763.5
31	46708.0
32	68079.0
33	93776.0
34	126812.0
35	180686.0
36	259495.0
37	352161.0
38	465652.5
39	609106.5
40	780383.0
41	966098.5
42	1157390.5
43	1351127.5
44	1502572.5
45	1595593.5
46	1629201.5
47	1603361.5
48	1555609.5
49	1505538.0
50	1401568.5
51	1242477.0
52	1063620.0
53	892867.5
54	752285.5
55	638150.0
56	530290.5
57	428949.5
58	354851.0
59	291029.5
60	221111.0
61	149300.5
62	91810.5
63	55627.0
64	34408.0
65	17258.0
66	9315.0
67	5111.0
68	2715.0
69	1615.5
70	945.0
71	739.5
72	506.0
73	384.0
74	314.0
75	200.5
76	131.5
77	46.5
78	10.0
79	3.5
80	3.0
81	0.5
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.143539329252969E-6
2	4.143539329252969E-6
3	0.0
4	0.0
5	0.0
6	4.143539329252969E-6
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.03288727165628082
25-29	0.0
30-34	8.287078658505939E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	3.3148314634023757E-6
55-59	0.0
60-64	0.0
65-69	1.6574157317011878E-6
70-74	0.0
75-79	0.0
80-84	0.0649582660646988
85-89	4.143539329252969E-6
90-94	8.287078658505939E-7
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.033367922218474164
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4133957E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.17550183484096
#Duplication Level	Percentage of total
1	24.686511608752483
2	8.337587541418486
3	5.20175393749097
4	3.777219336775249
5	2.8599063544450907
6	2.2264211500139597
7	1.853704689040561
8	1.5330379099204636
9	1.4211290173725526
>10	17.355308630203805
>50	6.0583330932157144
>100	12.997947124853251
>500	4.221964485133863
>1k	5.96129753713427
>5k	0.9241179936624452
>10k+	0.5837595905671987
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	28996	0.1201460663910191	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.3148314634023755E-4	4.143539329252969E-6	0.0	0.0	0.0022457983164551093	0.0
2	0.0018273008442005593	1.2430617987758907E-5	4.143539329252969E-6	0.0	0.009186226692953832	0.0
3	0.0026145733167586235	1.2430617987758907E-5	4.143539329252969E-6	0.0	0.012791105909403916	0.0
4	0.0063396151737570424	1.2430617987758907E-5	4.143539329252969E-6	0.0	0.01819428119474979	0.0
5	0.006542648600890438	1.2430617987758907E-5	4.143539329252969E-6	0.0	0.021989763220345508	0.0
6	0.007628255905154716	1.2430617987758907E-5	4.143539329252969E-6	0.0	0.027906737382518747	0.0
7	0.00983676236764655	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.037030810985533784	0.0
8	0.01752302782341081	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.049991802007437074	0.0
9	0.018202568273408295	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.05987414330770541	0.0
10-11	0.018909041729045922	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.09987172845298431	0.0
12-13	0.019727390746573384	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.11801007186678919	0.0
14-15	0.02269623667598314	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.12709478184617634	0.0
16-17	0.026272111117128454	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.13341782286261633	0.0
18-19	0.03343421884774221	3.522008429865023E-5	4.143539329252969E-6	0.0	0.14010549534003064	0.0
20-21	0.03970753739223121	3.9363623627903206E-5	4.143539329252969E-6	0.0	0.14467374745053205	0.0
22-23	0.04215429736615508	5.1794241615662115E-5	4.143539329252969E-6	0.0	0.1492855067239906	0.0
24-25	0.04777500846628674	6.008132027416805E-5	4.143539329252969E-6	0.0	0.15367973018266337	0.0
26-27	0.0527078920377624	7.458370792655344E-5	4.143539329252969E-6	0.0	0.15706085827533378	0.0
28-29	0.06385815637278214	7.458370792655344E-5	4.143539329252969E-6	2.0717696646264845E-6	0.16015193861495652	0.0
30-31	0.08792176102741875	7.458370792655344E-5	8.287078658505938E-6	4.143539329252969E-6	0.16361386572454736	0.0
32-33	0.10735703225127981	8.287078658505939E-5	8.287078658505938E-6	4.143539329252969E-6	0.16645633370441493	0.0
34-35	0.12144920951006916	8.701432591431236E-5	1.0358848323132422E-5	4.143539329252969E-6	0.16922214620669124	0.0
36-37	0.12380066807942022	9.322963490819181E-5	1.2430617987758907E-5	4.143539329252969E-6	0.17092099733168498	0.0
38-39	0.12599260038459503	9.53014045728183E-5	1.2430617987758907E-5	4.143539329252969E-6	0.1723940255632344	0.0
40-41	0.12740140375654105	9.737317423744478E-5	1.2430617987758907E-5	4.143539329252969E-6	0.17386291025545458	0.0
42-43	0.127679020891601	9.944494390207126E-5	1.2430617987758907E-5	4.143539329252969E-6	0.1752427088520958	0.0
44-45	0.12845179097650666	9.944494390207126E-5	1.2430617987758907E-5	4.143539329252969E-6	0.17650441657785337	0.0
46-47	0.12981087187650164	1.0358848323132423E-4	1.4502387652385392E-5	4.143539329252969E-6	0.17780755969690343	0.0
48-49	0.13109744083823469	1.0358848323132423E-4	1.6574157317011876E-5	4.143539329252969E-6	0.1788310139112289	0.0
50-51	0.13214368451887104	1.0358848323132423E-4	1.6574157317011876E-5	4.143539329252969E-6	0.18007821924933404	2.0717696646264845E-6
52-53	0.1331484928062149	1.0358848323132423E-4	1.6574157317011876E-5	4.143539329252969E-6	0.18126119972783578	4.143539329252969E-6
54-55	0.13367679407069466	1.0773202256057719E-4	1.6574157317011876E-5	6.2153089938794535E-6	0.18217485014993604	4.143539329252969E-6
56-57	0.13456351148715479	1.1187556188983016E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18317551489795064	4.143539329252969E-6
58-59	0.1347437554479773	1.222344102129626E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.1841596054886482	8.287078658505938E-6
60-61	0.13501930081337263	1.243061798775891E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18492616026456002	8.287078658505938E-6
62-63	0.13523476485849378	1.2844971920684205E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18577972936638615	8.287078658505938E-6
64-65	0.13525755432480469	1.388085675299745E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18669130801882178	8.287078658505938E-6
66-67	0.13536321457770062	1.4088033719460096E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18739363793513014	8.287078658505938E-6
68-69	0.1354398700552918	1.4295210685922742E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18805660422781062	8.287078658505938E-6
70-71	0.13552895615087074	1.5745449451161282E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18869056574518633	8.287078658505938E-6
72-73	0.135582822162151	1.6574157317011877E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.1891753598467089	1.2430617987758907E-5
74-75	0.13561389870712043	1.7402865182862472E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18973888119548732	1.6574157317011876E-5
76-77	0.13562425755544355	1.7817219115787768E-4	2.0717696646264847E-5	1.2430617987758907E-5	0.19038734510051541	1.6574157317011876E-5
78-79	0.13566154940940683	1.7817219115787768E-4	2.0717696646264847E-5	1.2430617987758907E-5	0.19133207206758512	1.6574157317011876E-5
80-81	0.13571127188135787	1.8231573048713064E-4	2.4861235975517814E-5	1.2430617987758907E-5	0.19215042108511254	2.0717696646264847E-5
82-83	0.13572991780833948	1.864592698163836E-4	2.6933005640144298E-5	1.4502387652385392E-5	0.1929045452430366	2.0717696646264847E-5
84-85	0.13577342497129666	1.9060280914563656E-4	2.9004775304770785E-5	1.6574157317011876E-5	0.19367317178861304	2.0717696646264847E-5
86-87	0.1358086450555953	2.0303342713339547E-4	2.9004775304770785E-5	2.0717696646264847E-5	0.19434856869928124	2.278946631089133E-5
88-89	0.13585215221855246	2.0924873612727495E-4	2.9004775304770785E-5	2.0717696646264847E-5	0.19512133878418694	2.4861235975517814E-5
90-91	0.13587908522419262	2.154640451211544E-4	2.9004775304770785E-5	2.0717696646264847E-5	0.19571179313860548	2.4861235975517814E-5
92-93	0.13588944407251574	2.154640451211544E-4	3.522008429865023E-5	2.0717696646264847E-5	0.19626495563906077	2.9004775304770785E-5
94-95	0.13593709477480215	2.154640451211544E-4	3.729185396327672E-5	2.4861235975517814E-5	0.19691756308341812	3.314831463402375E-5
96-97	0.13595366893211916	2.1753581478578087E-4	3.9363623627903206E-5	2.4861235975517814E-5	0.1975349504434768	4.143539329252969E-5
98-99	0.1359660995501069	2.278946631089133E-4	4.143539329252969E-5	2.4861235975517814E-5	0.19801767277533477	5.593778094491509E-5
100-101	0.1360013196344056	2.3203820243816628E-4	4.143539329252969E-5	2.4861235975517814E-5	0.19849417979819886	6.215308993879454E-5
102-103	0.13601582202205798	2.3203820243816628E-4	4.143539329252969E-5	2.4861235975517814E-5	0.19907220353462965	6.215308993879454E-5
104-105	0.1360427550276981	2.3203820243816628E-4	4.143539329252969E-5	2.4861235975517814E-5	0.19960879187776792	6.215308993879454E-5
106-107	0.13606761626367364	2.3203820243816628E-4	4.143539329252969E-5	2.4861235975517814E-5	0.20002521758035785	6.215308993879454E-5
108-109	0.136098692808643	2.3618174176741924E-4	4.143539329252969E-5	2.4861235975517814E-5	0.20052658583919744	6.422485960342102E-5
110-111	0.1361339128929417	2.486123597551782E-4	5.38660112802886E-5	2.4861235975517814E-5	0.20104867179468333	6.8368398932674E-5
112-113	0.1361587741289172	2.527558990844311E-4	5.38660112802886E-5	2.4861235975517814E-5	0.2015728295198338	7.458370792655344E-5
114-115	0.13618570713455735	2.568994384136841E-4	5.38660112802886E-5	2.4861235975517814E-5	0.20205762362135643	7.458370792655344E-5
116-117	0.13619192244355122	2.610429777429371E-4	5.38660112802886E-5	2.4861235975517814E-5	0.20255070480153753	7.665547759117992E-5
118-119	0.1361981377525451	2.69330056401443E-4	5.38660112802886E-5	2.4861235975517814E-5	0.2031204414593098	7.872724725580641E-5
120-121	0.13620849660086823	2.69330056401443E-4	5.38660112802886E-5	2.4861235975517814E-5	0.20371503935305763	7.872724725580641E-5
122-123	0.13622092721885598	2.69330056401443E-4	5.800955060954157E-5	2.4861235975517814E-5	0.20425369946586047	7.872724725580641E-5
124-125	0.13623750137617302	2.73473595730696E-4	5.800955060954157E-5	2.4861235975517814E-5	0.2048503691292729	8.07990169204329E-5
126-127	0.1362520037638254	2.73473595730696E-4	5.800955060954157E-5	2.6933005640144298E-5	0.2054035316297282	8.287078658505939E-5
128-129	0.13626650615147778	2.73473595730696E-4	5.800955060954157E-5	3.314831463402375E-5	0.20597948359649434	9.115786524356532E-5
130-131	0.1362768649998009	2.755453653953225E-4	6.0081320274168056E-5	3.314831463402375E-5	0.20664244988917482	9.115786524356532E-5
132-133	0.13628308030879477	2.817606743892019E-4	6.215308993879454E-5	3.314831463402375E-5	0.20728055494587977	9.322963490819181E-5
134-135	0.13628929561778866	2.817606743892019E-4	6.215308993879454E-5	3.314831463402375E-5	0.2078461480643228	1.0151671356669774E-4
136-137	0.13629343915711792	2.859042137184549E-4	6.215308993879454E-5	3.314831463402375E-5	0.20839516702544886	1.077320225605772E-4
138-139	0.1363162286234288	2.8797598338308134E-4	6.215308993879454E-5	3.314831463402375E-5	0.20894625775623948	1.1809087088370963E-4
>>END_MODULE
