##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.S.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24108909
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.79921318712514	37.0	35.0	38.0	33.0	39.0
2	35.34255092173603	35.0	34.0	37.0	33.0	39.0
3	35.51734468780815	35.0	34.0	37.0	33.0	39.0
4	35.560452237801385	36.0	35.0	38.0	33.0	39.0
5	35.474060356692206	36.0	35.0	37.0	33.0	39.0
6	35.42829101059695	35.0	35.0	37.0	33.0	39.0
7	35.47773791837698	35.0	34.0	38.0	33.0	39.0
8	35.57016366854261	36.0	35.0	38.0	33.0	39.0
9	35.54834049106079	36.0	35.0	38.0	33.0	39.0
10-14	35.533484796014626	35.8	34.8	37.8	33.0	39.0
15-19	35.51075098421086	36.0	35.0	38.0	33.0	39.0
20-24	35.501278552256345	36.0	35.0	38.0	33.0	39.0
25-29	35.48557916909471	36.0	34.6	38.0	33.0	39.0
30-34	35.47206821345586	36.0	34.4	38.0	33.0	39.0
35-39	35.44339049104213	36.0	34.0	38.0	33.0	39.0
40-44	35.43065434441682	36.0	34.0	38.0	33.0	39.0
45-49	35.40193306963828	36.0	34.0	38.0	33.0	39.0
50-54	35.38215127859995	36.0	34.0	38.0	32.6	39.0
55-59	35.35903270446622	36.0	34.0	38.0	32.2	39.0
60-64	35.31994561844338	36.0	34.0	38.0	32.0	39.0
65-69	35.29553077661042	36.0	34.0	38.0	32.0	39.0
70-74	35.26569651077948	36.0	34.0	38.0	32.0	39.0
75-79	35.21948561836622	36.0	34.0	38.0	32.0	39.0
80-84	35.179863626346595	36.0	34.0	38.0	32.0	39.0
85-89	35.13427927410569	36.0	34.0	38.0	32.0	39.0
90-94	35.08502332478007	36.0	34.0	38.0	32.0	39.0
95-99	35.02133417982539	35.2	34.0	38.0	32.0	39.0
100-104	34.9757630592077	35.2	34.0	38.0	31.0	39.0
105-109	34.92226467817353	35.0	34.0	37.6	31.0	39.0
110-114	34.84788880326356	35.0	34.0	37.0	31.0	39.0
115-119	34.776792993826476	35.0	34.0	37.0	31.0	39.0
120-124	34.70675239597114	35.0	34.0	37.0	30.6	39.0
125-129	34.63100992251454	35.0	34.0	37.0	30.0	39.0
130-134	34.52788936239296	35.0	34.0	37.0	30.0	39.0
135-139	34.47438971211845	35.0	34.0	37.0	30.0	39.0
140-144	34.352742407381434	35.0	34.0	37.0	29.8	39.0
145-149	34.275280121551745	35.0	34.0	37.0	29.2	39.0
150	34.215826688797904	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	14.0
21	257.0
22	1987.0
23	9829.0
24	31990.0
25	76381.0
26	145855.0
27	242723.0
28	353860.0
29	469528.0
30	609735.0
31	803148.0
32	1083286.0
33	1522708.0
34	2295508.0
35	3895923.0
36	1.0271573E7
37	2294601.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.257618210761834	18.825339628599536	14.032683934391224	32.8843582262474
2	23.919444052818815	26.465863718677607	32.587007566373074	17.0276846621305
3	19.645264744248692	29.638085240605456	29.072937311265306	21.643712703880542
4	23.16438292583045	34.432478881562	21.4989197561781	20.904218436429453
5	25.212443250750166	34.97586721987295	21.84862035855708	17.963069170819796
6	19.063318045623713	38.93324662679676	22.171563217564096	19.831872110015432
7	20.376591906336365	20.681798583254015	36.82175331948866	22.11985619092096
8	21.53211910169805	23.50334061155567	28.158072188169115	26.80646809857717
9	23.463778472928826	23.397010623749086	27.908463215817854	25.230747687504234
10-14	23.160157931659207	28.68924346597351	25.1988524242221	22.951746178145182
15-19	23.177049612655637	27.694495839691456	26.672251324188913	22.45620322346399
20-24	23.10949948004698	27.603962501994594	26.620029135287705	22.666508882670716
25-29	23.152546637427683	27.24933260148769	26.598292772186415	22.99982798889821
30-34	23.014478174852293	26.93026465859571	26.80663567148559	23.24862149506641
35-39	23.3069096847718	26.695756549545703	26.82805619141087	23.169277574271632
40-44	23.50827820537213	26.28657729804364	26.88357403481012	23.32157046177411
45-49	23.387561834506904	26.116815157417534	26.997629797350015	23.497993210725546
50-54	23.620162156653375	26.12936902287864	26.709537125881553	23.54093169458643
55-59	23.65849072639496	26.13732458818439	26.715085282374247	23.489099403046403
60-64	23.35977459618766	26.12579026284433	26.797442389450303	23.716992751517708
65-69	23.565341953593517	26.030133723845683	26.766731972539542	23.63779235002125
70-74	23.75584560877475	26.017048718380416	26.456046600864436	23.771059071980403
75-79	23.594852010930897	26.112138048221095	26.709679811724374	23.583330129123638
80-84	23.901490523689812	26.124297868476752	26.391042415067396	23.583169192766043
85-89	23.6638879013563	26.21961864802758	26.582966487616673	23.533526962999446
90-94	23.668675343210264	25.995772765992854	26.604766727519692	23.730785163277194
95-99	23.718512521657452	26.160516844623704	26.63813862336118	23.482832010357665
100-104	23.95348872900055	25.967423079990887	26.586524508429644	23.49256368257892
105-109	23.71581808202105	26.156218018824497	26.503234136393317	23.62472976276114
110-114	23.822508932278936	26.234783166670876	26.42774834813139	23.5149595529188
115-119	23.97101585973882	26.331239625982246	26.565413640243946	23.13233087403499
120-124	23.92132634454757	26.492246496927752	26.693147334041534	22.89327982448314
125-129	23.906638827995078	26.530622352093992	26.529607789386073	23.033131030524856
130-134	24.077277413542767	26.542065979345402	26.509397422670173	22.87125918444166
135-139	23.87402847636117	26.556284235010384	26.775105418499027	22.79458187012942
140-144	23.913355017433595	26.696212590955486	26.577021797211977	22.813410594398942
145-149	24.020222565857292	27.115348106378434	26.211269867085235	22.65315946067904
150	23.8919314017901	27.117398800584464	26.13277938043567	22.85789041718976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.5
7	1.0
8	1.5
9	2.0
10	4.0
11	5.0
12	5.5
13	12.5
14	20.0
15	31.0
16	54.0
17	94.0
18	174.5
19	289.0
20	435.0
21	645.0
22	1004.0
23	1596.5
24	2583.5
25	4087.5
26	6341.0
27	9822.5
28	14786.5
29	21127.5
30	30176.5
31	44075.0
32	64222.0
33	88541.0
34	120336.5
35	173194.0
36	249654.0
37	339805.0
38	453620.0
39	597369.5
40	767315.5
41	953435.0
42	1146670.5
43	1340452.0
44	1490530.0
45	1588889.0
46	1625582.5
47	1597853.0
48	1555223.0
49	1504210.5
50	1399267.0
51	1245346.0
52	1073266.5
53	906990.0
54	763042.5
55	650697.0
56	544946.5
57	440318.0
58	365076.0
59	302714.0
60	232283.5
61	157950.0
62	97456.0
63	59012.5
64	36316.0
65	17602.5
66	9280.5
67	5041.5
68	2621.0
69	1661.5
70	1049.5
71	872.5
72	584.0
73	426.0
74	359.0
75	223.0
76	149.0
77	59.5
78	15.5
79	2.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.295688535719307E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.295688535719307E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.05627961016402692
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4108909E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.21021688083598
#Duplication Level	Percentage of total
1	24.89729155435475
2	8.16815246924167
3	5.171593103527308
4	3.6953529009873565
5	2.7920964787678466
6	2.208023504345887
7	1.7649562365237015
8	1.5290979783280332
9	1.3271616734291913
>10	16.92344457620208
>50	5.907532517104045
>100	13.196973818863714
>500	4.379545201251902
>1k	6.265797427457069
>5k	0.9660774595867505
>10k+	0.8069031000285403
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	28499	0.11820941378973225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.069404758216143E-4	4.147844267859653E-6	0.0	0.0	0.0016466941743402822	0.0
2	0.0014849282478937558	1.2443532803578959E-5	0.0	0.0	0.008868091044683939	0.0
3	0.002219096683304914	1.2443532803578959E-5	0.0	0.0	0.012742177590864854	0.0
4	0.005437823835164005	1.2443532803578959E-5	0.0	4.147844267859653E-6	0.01864455998402914	0.0
5	0.005665955269896286	1.2443532803578959E-5	4.147844267859653E-6	4.147844267859653E-6	0.0227094473665316	0.0
6	0.006731951246736217	1.2443532803578959E-5	4.147844267859653E-6	4.147844267859653E-6	0.02986862657285736	0.0
7	0.008913717331630395	1.2443532803578959E-5	4.147844267859653E-6	4.147844267859653E-6	0.04113002376009632	0.0
8	0.017371171793796228	1.2443532803578959E-5	4.147844267859653E-6	4.147844267859653E-6	0.05430772499908644	0.0
9	0.01816341004895742	1.6591377071438612E-5	4.147844267859653E-6	4.147844267859653E-6	0.06405930687282448	0.0
10-11	0.018955648304118614	1.6591377071438612E-5	4.147844267859653E-6	4.147844267859653E-6	0.10394912519683076	0.0
12-13	0.019946983084137074	1.6591377071438612E-5	4.147844267859653E-6	4.147844267859653E-6	0.12346888032137829	0.0
14-15	0.023881213372201955	2.0739221339298265E-5	4.147844267859653E-6	4.147844267859653E-6	0.1333635628223575	0.0
16-17	0.028130679824624166	2.0739221339298265E-5	4.147844267859653E-6	4.147844267859653E-6	0.13994411775331683	0.0
18-19	0.03566109109292337	2.4887065607157918E-5	4.147844267859653E-6	4.147844267859653E-6	0.14639608951197253	0.0
20-21	0.0417750135437485	2.4887065607157918E-5	4.147844267859653E-6	4.147844267859653E-6	0.15102300979276997	0.0
22-23	0.04452296037120551	3.3182754142877224E-5	4.147844267859653E-6	4.147844267859653E-6	0.15568726067197813	0.0
24-25	0.0511138849128345	3.3182754142877224E-5	4.147844267859653E-6	8.295688535719306E-6	0.1601752281698023	0.0
26-27	0.057240250896463216	3.3182754142877224E-5	4.147844267859653E-6	8.295688535719306E-6	0.1637548177729652	0.0
28-29	0.07123715137835561	3.525667627680705E-5	4.147844267859653E-6	8.295688535719306E-6	0.16729707677771732	0.0
30-31	0.09838479211149705	3.733059841073688E-5	2.0739221339298265E-5	8.295688535719306E-6	0.1712644898199251	0.0
32-33	0.12015475275135842	3.733059841073688E-5	2.0739221339298265E-5	8.295688535719306E-6	0.1740808760778018	0.0
34-35	0.13586886076014473	3.94045205446667E-5	2.0739221339298265E-5	8.295688535719306E-6	0.17706732395066072	0.0
36-37	0.13853799854651241	4.147844267859653E-5	2.0739221339298265E-5	8.295688535719306E-6	0.17894007563759934	4.147844267859653E-6
38-39	0.1409561917546746	4.562628694645618E-5	2.0739221339298265E-5	8.295688535719306E-6	0.1803835254428145	4.147844267859653E-6
40-41	0.14262362515035415	4.9774131214315836E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18189956252271722	4.147844267859653E-6
42-43	0.14297619191312222	4.9774131214315836E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18336582547140562	4.147844267859653E-6
44-45	0.14384309136510492	5.5995897616105313E-5	2.0739221339298265E-5	8.295688535719306E-6	0.184595661296826	8.295688535719306E-6
46-47	0.14506463150198956	6.221766401789479E-5	2.0739221339298265E-5	8.295688535719306E-6	0.1858752712534607	8.295688535719306E-6
48-49	0.14619491906498133	7.05133525536141E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18701800234925603	8.295688535719306E-6
50-51	0.14733142839437488	7.466119682147376E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18812340284664064	8.295688535719306E-6
52-53	0.1485301553877863	7.88090410893334E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18919354666774843	8.295688535719306E-6
54-55	0.14904034023273305	8.50308074911229E-5	2.4887065607157918E-5	8.295688535719306E-6	0.19008533318533824	8.295688535719306E-6
56-57	0.14980354357801923	8.917865175898254E-5	2.4887065607157918E-5	1.2443532803578959E-5	0.19110155503096388	8.295688535719306E-6
58-59	0.1499404224388586	9.33264960268422E-5	3.11088320089474E-5	1.2443532803578959E-5	0.1919124585853304	8.295688535719306E-6
60-61	0.1501975887834659	1.0162218456256149E-4	3.733059841073688E-5	1.2443532803578959E-5	0.1926196660330005	1.2443532803578959E-5
62-63	0.1505314902470286	1.0369610669649133E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19343264350950098	1.2443532803578959E-5
64-65	0.15056259907903755	1.1199179523221063E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19420829038759074	1.2443532803578959E-5
66-67	0.15063726027585903	1.2443532803578958E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19480765388429647	1.4517454937508785E-5
68-69	0.1507305867718859	1.327310165715089E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19549412211062725	1.6591377071438612E-5
70-71	0.15084880033351986	1.327310165715089E-4	3.733059841073688E-5	1.2443532803578959E-5	0.196062376775324	1.6591377071438612E-5
72-73	0.1508923526983324	1.327310165715089E-4	3.733059841073688E-5	1.2443532803578959E-5	0.1965767094645386	1.6591377071438612E-5
74-75	0.15091723976393956	1.327310165715089E-4	3.733059841073688E-5	1.2443532803578959E-5	0.1972299949367265	1.6591377071438612E-5
76-77	0.15095664428448421	1.3895278297329836E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19787291079824476	1.6591377071438612E-5
78-79	0.15101886194850211	1.6176592644652648E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19863611414353094	1.6591377071438612E-5
80-81	0.15108107961252001	1.721355371161756E-4	4.147844267859653E-5	1.2443532803578959E-5	0.19938272611174568	1.6591377071438612E-5
82-83	0.1511018188338593	1.7835730351796507E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20018326005544257	1.6591377071438612E-5
84-85	0.15114122335440394	1.8250514778582474E-4	4.147844267859653E-5	1.2443532803578959E-5	0.2008655804375055	1.6591377071438612E-5
86-87	0.15118477571921649	1.8250514778582474E-4	4.147844267859653E-5	1.2443532803578959E-5	0.2015126441432916	1.6591377071438612E-5
88-89	0.15122210631762723	1.866529920536844E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20226133003364027	1.6591377071438612E-5
90-91	0.15122625416189509	1.866529920536844E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20274870173511378	1.6591377071438612E-5
92-93	0.15124284553896652	1.866529920536844E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20318422538323905	1.6591377071438612E-5
94-95	0.15127395437097546	1.949486805894037E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20377529319140902	1.6591377071438612E-5
96-97	0.15128017613737726	2.0531829125905283E-4	4.147844267859653E-5	1.2443532803578959E-5	0.2043352521675701	1.6591377071438612E-5
98-99	0.1512946935923148	2.115400576608423E-4	4.562628694645618E-5	1.2443532803578959E-5	0.20494498527494548	1.6591377071438612E-5
100-101	0.151317506735788	2.115400576608423E-4	4.9774131214315836E-5	1.6591377071438612E-5	0.20547798326336542	1.6591377071438612E-5
102-103	0.15132372850218978	2.1568790192870197E-4	4.9774131214315836E-5	1.6591377071438612E-5	0.20602135086245504	1.866529920536844E-5
104-105	0.1513361720349934	2.177618240626318E-4	4.9774131214315836E-5	1.6591377071438612E-5	0.20650042687539283	2.0739221339298265E-5
106-107	0.15135691125633266	2.198357461965616E-4	5.1848053348245664E-5	1.866529920536844E-5	0.2070002421096699	2.0739221339298265E-5
108-109	0.1513735026334041	2.447228118037195E-4	5.392197548217549E-5	2.0739221339298265E-5	0.207471022434072	2.0739221339298265E-5
110-111	0.15140875930968092	2.447228118037195E-4	5.806981975003514E-5	2.0739221339298265E-5	0.20798950296755445	2.0739221339298265E-5
112-113	0.1514274246088863	2.4679673393764934E-4	5.806981975003514E-5	2.0739221339298265E-5	0.2085577576322512	2.0739221339298265E-5
114-115	0.15144401598595775	2.50944578205509E-4	5.806981975003514E-5	2.0739221339298265E-5	0.20911564268627833	2.0739221339298265E-5
116-117	0.1514564595187613	2.592402667412283E-4	5.806981975003514E-5	2.0739221339298265E-5	0.20956360986720718	2.281314347322809E-5
118-119	0.15145853344089524	2.6131418887515815E-4	5.806981975003514E-5	2.4887065607157918E-5	0.21002609450307352	2.903490987501757E-5
120-121	0.1514606073630292	2.6131418887515815E-4	5.806981975003514E-5	2.4887065607157918E-5	0.2105466489586899	2.903490987501757E-5
122-123	0.15147097697369882	2.6131418887515815E-4	5.806981975003514E-5	2.4887065607157918E-5	0.21102365104949378	2.903490987501757E-5
124-125	0.15148342050650238	2.6131418887515815E-4	5.806981975003514E-5	2.6960987741087746E-5	0.21151102275096728	2.903490987501757E-5
126-127	0.15150415972784168	2.6131418887515815E-4	5.806981975003514E-5	2.903490987501757E-5	0.21201498582951223	2.903490987501757E-5
128-129	0.15151452933851134	2.6131418887515815E-4	5.806981975003514E-5	2.903490987501757E-5	0.212583240494209	3.733059841073688E-5
130-131	0.15152489894918098	2.737577216787371E-4	6.014374188396497E-5	2.903490987501757E-5	0.21324689557706655	3.733059841073688E-5
132-133	0.1515394164041185	2.737577216787371E-4	6.221766401789479E-5	2.903490987501757E-5	0.2137923370982901	3.733059841073688E-5
134-135	0.15155808170332385	2.820534102144564E-4	6.221766401789479E-5	2.903490987501757E-5	0.21429215233256718	3.94045205446667E-5
136-137	0.1515808948467971	2.820534102144564E-4	6.221766401789479E-5	3.1108832008947396E-5	0.21481063286604965	4.5626286946456186E-5
138-139	0.1516099297566721	3.007187094198248E-4	6.221766401789479E-5	3.3182754142877224E-5	0.21532289163313031	4.5626286946456186E-5
>>END_MODULE
