##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21817240
Total Bases	3.2 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.73222139922373	37.0	35.0	38.0	33.0	39.0
2	35.371914045956316	35.0	34.0	37.0	33.0	39.0
3	35.48401708923769	35.0	34.0	37.0	33.0	39.0
4	35.55473703364862	36.0	35.0	38.0	33.0	39.0
5	35.473243774189584	36.0	35.0	37.0	33.0	39.0
6	35.4260037474951	35.0	35.0	37.0	33.0	39.0
7	35.44953724669115	35.0	34.0	37.0	33.0	39.0
8	35.53282619616414	36.0	35.0	38.0	33.0	39.0
9	35.53649719212879	36.0	35.0	38.0	33.0	39.0
10-14	35.52266592841258	35.8	35.0	37.8	33.0	39.0
15-19	35.500985679215155	36.0	35.0	38.0	33.0	39.0
20-24	35.482444763865644	36.0	35.0	38.0	33.0	39.0
25-29	35.46523631770105	36.0	35.0	38.0	33.0	39.0
30-34	35.44298151370201	36.0	34.4	38.0	33.0	39.0
35-39	35.41769849898521	36.0	34.0	38.0	33.0	39.0
40-44	35.39597487124861	36.0	34.0	38.0	33.0	39.0
45-49	35.36707512040937	36.0	34.0	38.0	33.0	39.0
50-54	35.3418505273811	36.0	34.0	38.0	32.6	39.0
55-59	35.31507938676019	36.0	34.0	38.0	32.2	39.0
60-64	35.2706181258491	36.0	34.0	38.0	32.0	39.0
65-69	35.24244071202407	36.0	34.0	38.0	32.0	39.0
70-74	35.21056409518344	36.0	34.0	38.0	32.0	39.0
75-79	35.160425296691976	35.8	34.0	37.6	32.0	39.0
80-84	35.117857373343284	35.4	34.0	37.2	32.0	39.0
85-89	35.06620824632263	35.0	34.0	37.0	32.0	39.0
90-94	35.00889768825021	35.0	34.0	37.0	32.0	39.0
95-99	34.9422053751987	35.0	34.0	37.0	31.0	39.0
100-104	34.891085902708134	35.0	34.0	37.0	31.0	39.0
105-109	34.82798855400591	35.0	34.0	37.0	31.0	39.0
110-114	34.75196374060147	35.0	34.0	37.0	31.0	39.0
115-119	34.687583186507545	35.0	34.0	37.0	30.8	39.0
120-124	34.61119567828011	35.0	34.0	37.0	30.0	39.0
125-129	34.530307133257914	35.0	34.0	37.0	30.0	39.0
130-134	34.4278636069457	35.0	34.0	37.0	30.0	39.0
135-139	34.36434184158949	35.0	34.0	37.0	30.0	39.0
140-144	34.23634221377223	35.0	34.0	37.0	29.0	39.0
145-149	34.15859864950837	35.0	34.0	37.0	29.0	39.0
150	34.08473982043558	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	15.0
21	167.0
22	1680.0
23	8340.0
24	27275.0
25	65426.0
26	128037.0
27	218904.0
28	323372.0
29	432519.0
30	566225.0
31	748483.0
32	1013735.0
33	1426166.0
34	2156330.0
35	3719750.0
36	9530446.0
37	1450360.0
38	10.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.0970049373798	19.266396666122752	16.414088124804056	31.222510271693395
2	24.6652188819484	25.71055733905847	32.582095627127906	17.04212815186522
3	19.962382959531087	29.726986548252665	30.31255557531567	19.99807491690058
4	23.563690915991206	33.95082512728466	22.627247992871695	19.85823596385244
5	25.360604732770962	35.36990471755364	22.441587478526156	16.82790307114924
6	18.868729500156757	39.623614169344975	22.578222543273117	18.929433787225147
7	20.252167551899326	21.25484708423247	38.094616917630276	20.39836844623793
8	21.234487025545647	23.773082842063236	28.60062692719817	26.39180320519295
9	23.307040670588947	23.227869336359685	29.18623070562546	24.27885928742591
10-14	22.923882214248913	29.52167918581819	25.833281386646522	21.721157213286375
15-19	22.881436882025408	28.36113550568266	27.284134015118322	21.473293597173612
20-24	22.94347314325735	28.459221239716847	27.15887344137022	21.438432175655585
25-29	23.03040989602718	28.340087013756094	27.076606390175844	21.552896700040886
30-34	23.02000894705288	28.16904338037259	27.243618349525423	21.567329323049112
35-39	23.142956670962963	28.129658930277156	27.176648375321534	21.550736023438343
40-44	23.279861247343845	27.946700865920715	27.26834008334693	21.505097803388512
45-49	23.295988860185798	27.772725605988658	27.345247153168778	21.586038380656767
50-54	23.36773579059496	27.719486974521068	27.3049258292983	21.607851405585674
55-59	23.514152110899452	27.584844829135125	27.24091314941762	21.660089910547807
60-64	23.42018972152298	27.57048829274464	27.28953616497779	21.719785820754595
65-69	23.517110321928897	27.494050576516553	27.26552396178435	21.7233151397702
70-74	23.617284312772835	27.415548437840897	27.168464022030285	21.798703227355983
75-79	23.531925211438292	27.482159979905802	27.249160755439277	21.736754053216632
80-84	23.65308077465344	27.528006292271616	27.080693983290278	21.738218949784667
85-89	23.67213543051275	27.50254294310371	27.148953763170773	21.67636786321276
90-94	23.618641954711045	27.416962915565858	27.201082263384368	21.76331286633873
95-99	23.670577029908458	27.533403858599897	27.20245182250367	21.593567288987973
100-104	23.872143314186395	27.474503649407534	27.009784922382487	21.64356811402359
105-109	23.629804903184866	27.61728548264845	27.133798107678132	21.619111506488554
110-114	23.67835865592772	27.601273048501046	27.05171126429376	21.668657031277476
115-119	23.737577255418195	27.602726101010028	27.092240815061853	21.567455828509928
120-124	23.6770911444344	27.52900550207084	27.243219582311966	21.55068377118279
125-129	23.66967315755797	27.619841006470114	27.20489117780251	21.50559465816941
130-134	23.73591747278409	27.516791837400202	27.279797500745474	21.467493189070233
135-139	23.607081372345906	27.528792826223665	27.393155137863452	21.470970663566977
140-144	23.64978521572848	27.658024571393998	27.339316980516326	21.352873232361198
145-149	23.77370303277319	28.241945619537077	26.78685687820143	21.197494469488298
150	23.37362562817295	28.178243444175337	26.928837011464328	21.519293916187383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	3.0
9	8.0
10	6.5
11	6.0
12	11.0
13	14.0
14	24.5
15	46.5
16	82.5
17	143.5
18	252.0
19	434.0
20	670.0
21	1100.5
22	1744.0
23	2808.5
24	4670.5
25	7579.5
26	11660.5
27	18439.5
28	29806.5
29	44038.0
30	58745.5
31	76709.5
32	102216.5
33	134706.0
34	177337.0
35	241715.0
36	336121.5
37	449320.5
38	581807.0
39	753369.0
40	951943.0
41	1129772.5
42	1285315.5
43	1434421.5
44	1539408.0
45	1580430.5
46	1567282.5
47	1507374.5
48	1408691.5
49	1274013.0
50	1105601.5
51	925317.5
52	753342.5
53	598017.5
54	465318.5
55	351034.5
56	258596.0
57	191338.0
58	140082.0
59	100186.5
60	69050.5
61	45665.0
62	30997.5
63	21433.0
64	14249.5
65	9388.5
66	6525.0
67	4908.0
68	4373.5
69	3183.5
70	1638.0
71	1048.0
72	554.5
73	359.0
74	323.5
75	222.5
76	144.0
77	63.0
78	21.5
79	3.0
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.11420784663871324
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	9.167062378192658E-7
110-114	0.01902715467217668
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.047235122316113314
135-139	0.0
140-144	0.0
145-149	9.167062378192658E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.181724E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.309450637536486
#Duplication Level	Percentage of total
1	29.643423786056506
2	11.15260649448867
3	7.147162681949604
4	5.221746104456596
5	3.9947941704670122
6	3.0588136753516055
7	2.52907062300672
8	2.2012045565596514
9	1.7946490620857642
>10	20.832949659757976
>50	5.0276628998572646
>100	5.675616019499096
>500	0.8122772927251795
>1k	0.838705393285462
>5k	0.06931758045267908
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.3750593567288987E-5	4.5835311890963294E-6	0.0	0.0	6.737790847971604E-4	0.0
2	3.2084718323674305E-5	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.00520689143081343	0.0
3	5.041884308005962E-5	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.007040303906451962	0.0
4	1.1917181091650456E-4	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.008502450355773692	0.0
5	1.237553421056009E-4	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.01022585808287391	0.0
6	1.2833887329469722E-4	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.012109689401592502	0.0
7	1.3292240448379354E-4	3.2084718323674305E-5	0.0	4.5835311890963294E-6	0.014089774875282117	0.0
8	1.8334124756385318E-4	3.6668249512770636E-5	0.0	4.5835311890963294E-6	0.0199796124532709	0.0
9	1.9250830994204583E-4	4.5835311890963296E-5	0.0	4.5835311890963294E-6	0.026314052556602026	0.0
10-11	2.1771773148207562E-4	4.812707748551146E-5	0.0	4.5835311890963294E-6	0.03298079867114264	0.0
12-13	2.520942154002981E-4	5.729413986370411E-5	0.0	4.5835311890963294E-6	0.03887521978032052	0.0
14-15	2.8188716812942426E-4	6.416943664734861E-5	0.0	4.5835311890963294E-6	0.04749455018141616	0.0
16-17	3.116801208585504E-4	6.416943664734861E-5	0.0	4.5835311890963294E-6	0.05336376186905402	0.0
18-19	3.643907295331582E-4	6.875296783644493E-5	0.0	4.5835311890963294E-6	0.06179745925699126	0.0
20-21	4.010589790459288E-4	7.562826462008943E-5	0.0	4.5835311890963294E-6	0.0681089817043769	0.0
22-23	4.377272285586995E-4	1.0083768616011924E-4	0.0	4.5835311890963294E-6	0.07413861698363314	0.0
24-25	4.789790092605664E-4	1.0771298294376374E-4	0.0	4.5835311890963294E-6	0.07949447317809219	0.0
26-27	5.133554931787889E-4	1.1458827972740824E-4	0.0	9.167062378192659E-6	0.08331255465860943	0.0
28-29	6.096096481498118E-4	1.1917181091650457E-4	0.0	1.1458827972740822E-5	0.08755232100852353	0.0
30-31	7.585744117954425E-4	1.2833887329469722E-4	0.0	1.3750593567288987E-5	0.09228710872686005	0.0
32-33	8.777462227119471E-4	1.5125652924017887E-4	0.0	1.3750593567288987E-5	0.09711815060016757	0.0
34-35	9.831674400611627E-4	1.5813182602382336E-4	0.0	1.3750593567288987E-5	0.10025099416791491	0.0
36-37	0.0010221274551684813	1.627153572129197E-4	0.0	1.3750593567288987E-5	0.10377343788673545	0.0
38-39	0.00108629689181583	1.6500712280746786E-4	0.0	1.3750593567288987E-5	0.10715150037309944	0.0
40-41	0.001127548672517697	1.695906539965642E-4	0.0	1.3750593567288987E-5	0.1100184991318792	0.0
42-43	0.0011367157348958897	1.81049481969305E-4	0.0	1.8334124756385318E-5	0.1129405002649281	0.0
44-45	0.001182551046786853	1.9480007553659398E-4	0.0	1.8334124756385318E-5	0.11530331059290727	0.0
46-47	0.0012238028274887199	2.4063538742755727E-4	2.2917655945481647E-6	1.8334124756385318E-5	0.11792509043307037	0.0
48-49	0.0012467204834342017	2.589695121839426E-4	4.5835311890963294E-6	1.8334124756385318E-5	0.12031769371377865	4.5835311890963294E-6
50-51	0.0013017228577033575	2.635530433730389E-4	4.5835311890963294E-6	1.8334124756385318E-5	0.12249028749741031	4.5835311890963294E-6
52-53	0.0013544334663779653	2.727201057512316E-4	4.5835311890963294E-6	1.8334124756385318E-5	0.1246789236402038	4.5835311890963294E-6
54-55	0.0013888099502961878	2.8647069931852056E-4	4.5835311890963294E-6	1.8334124756385318E-5	0.1264642090383568	4.5835311890963294E-6
56-57	0.0014369370277816994	2.910542305076169E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.12862763575961028	4.5835311890963294E-6
58-59	0.001501106464429048	2.979295272912614E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.1306214718268672	4.5835311890963294E-6
60-61	0.00158590179142733	3.048048240749059E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.1321065359321344	6.875296783644494E-6
62-63	0.001629445337723745	3.1397188645309855E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.13424475323184784	9.167062378192659E-6
64-65	0.0016729888840201603	3.2772248002038755E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.1362317140023211	9.167062378192659E-6
66-67	0.0017646595078020869	3.391813079931284E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.13760906512464455	9.167062378192659E-6
68-69	0.0018036195229094057	3.391813079931284E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.13916517396334277	9.167062378192659E-6
70-71	0.0018196618820712426	3.506401359658692E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.14069378161490637	1.3750593567288987E-5
72-73	0.0018425795380167245	3.5751543274951367E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.141674657289373	1.3750593567288987E-5
74-75	0.0018471630692058207	3.6209896393861E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.14281595655545798	1.3750593567288987E-5
76-77	0.0018723724907458504	3.75849557505899E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.14420705827134872	1.3750593567288987E-5
78-79	0.0019182078026368137	4.010589790459288E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.1457287906261287	1.3750593567288987E-5
80-81	0.001920499568231362	4.1480957261321783E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.14718177001307223	1.3750593567288987E-5
82-83	0.0019250830994204584	4.354354629641513E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.1488639259594706	1.3750593567288987E-5
84-85	0.0019388336929877473	4.377272285586995E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.15065608665440725	1.6042359161837153E-5
86-87	0.001975501942500518	4.400189941532476E-4	9.167062378192659E-6	2.7501187134577975E-5	0.1525422097387204	2.0625890350933483E-5
88-89	0.0019984195984459998	4.468942909368921E-4	1.3750593567288987E-5	2.7501187134577975E-5	0.15439624810470987	2.2917655945481648E-5
90-91	0.0020144619576078367	4.812707748551146E-4	1.3750593567288987E-5	2.7501187134577975E-5	0.15548483676212022	3.2084718323674305E-5
92-93	0.0020282125511751257	4.812707748551146E-4	2.2917655945481648E-5	2.7501187134577975E-5	0.15648633832693779	3.2084718323674305E-5
94-95	0.00203508784795877	4.858543060442109E-4	2.2917655945481648E-5	2.7501187134577975E-5	0.15757492698434816	3.6668249512770636E-5
96-97	0.002044254910336963	4.858543060442109E-4	2.2917655945481648E-5	2.7501187134577975E-5	0.15865893211056945	3.6668249512770636E-5
98-99	0.002055713738309704	4.973131340169517E-4	2.5209421540029813E-5	2.7501187134577975E-5	0.1596031395355233	3.8960015107318804E-5
100-101	0.002062589035093348	4.996048996114999E-4	2.7501187134577975E-5	2.7501187134577975E-5	0.1604992198829916	4.1251780701866966E-5
102-103	0.0020671725662824445	4.996048996114999E-4	3.2084718323674305E-5	2.7501187134577975E-5	0.16154655675970014	4.1251780701866966E-5
104-105	0.002074047863066089	5.041884308005962E-4	3.6668249512770636E-5	2.7501187134577975E-5	0.16248847241905942	4.5835311890963296E-5
106-107	0.0021084243469843113	5.179390243678852E-4	3.6668249512770636E-5	2.7501187134577975E-5	0.16328600684596217	4.812707748551146E-5
108-109	0.0021152996437679558	5.225225555569816E-4	4.5835311890963296E-5	2.7501187134577975E-5	0.1641293765847559	5.271060867460779E-5
110-111	0.0021267584717406967	5.339813835297223E-4	5.041884308005962E-5	2.7501187134577975E-5	0.16507358400970976	5.729413986370411E-5
112-113	0.0021267584717406967	5.385649147188188E-4	5.041884308005962E-5	2.7501187134577975E-5	0.16589403609255798	6.416943664734861E-5
114-115	0.0021428008309025337	5.500237426915595E-4	5.041884308005962E-5	2.7501187134577975E-5	0.1667557399561081	6.416943664734861E-5
116-117	0.002145092596497082	5.591908050697522E-4	5.729413986370411E-5	2.7501187134577975E-5	0.16771828150581836	6.416943664734861E-5
118-119	0.0021496761276861786	5.591908050697522E-4	5.958590545825228E-5	2.7501187134577975E-5	0.16857081830699025	7.792003021463761E-5
120-121	0.0021496761276861786	5.683578674479449E-4	5.958590545825228E-5	2.7501187134577975E-5	0.16947148218564767	8.708709259283026E-5
122-123	0.0021542596588752746	5.70649633042493E-4	5.958590545825228E-5	2.7501187134577975E-5	0.17030110133087412	8.708709259283026E-5
124-125	0.00217259378363166	5.798166954206856E-4	5.958590545825228E-5	2.979295272912614E-5	0.1712613511149898	9.167062378192659E-5
126-127	0.0021886361427934974	5.866919922043302E-4	5.958590545825228E-5	3.2084718323674305E-5	0.17224910208624006	9.625415497102292E-5
128-129	0.0022069702675498824	5.889837577988783E-4	5.958590545825228E-5	3.2084718323674305E-5	0.17320935187035574	9.625415497102292E-5
130-131	0.0022115537987389793	6.027343513661672E-4	5.958590545825228E-5	3.2084718323674305E-5	0.17427502287182062	1.0542121734921558E-4
132-133	0.0022275961579008158	6.119014137443599E-4	6.187767105280045E-5	3.2084718323674305E-5	0.17517568675047807	1.0771298294376374E-4
134-135	0.0022459302826572016	6.187767105280044E-4	6.416943664734861E-5	3.2084718323674305E-5	0.1761107271130537	1.100047485383119E-4
136-137	0.002255097345035394	6.233602417171008E-4	6.416943664734861E-5	3.2084718323674305E-5	0.17709847808430396	1.1458827972740824E-4
138-139	0.002271139704197231	6.41694366473486E-4	6.416943664734861E-5	3.2084718323674305E-5	0.1779326807607195	1.1458827972740824E-4
>>END_MODULE
