##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23004401
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.84736407611744	37.0	35.0	38.0	33.0	39.0
2	35.39674295366352	35.0	34.0	37.0	33.0	39.0
3	35.517166649981455	35.0	34.0	37.0	33.0	39.0
4	35.61277005213046	36.0	35.0	38.0	33.0	39.0
5	35.542586872833596	36.0	35.0	37.0	33.0	39.0
6	35.47608181582298	36.0	35.0	37.0	33.0	39.0
7	35.43991530142428	35.0	34.0	37.0	33.0	39.0
8	35.59467777491794	36.0	35.0	38.0	33.0	39.0
9	35.56723224395193	36.0	35.0	38.0	33.0	39.0
10-14	35.493540857681964	36.0	34.8	37.8	33.0	39.0
15-19	35.49797132296555	36.0	34.8	38.0	33.0	39.0
20-24	35.47410608952609	36.0	34.0	38.0	33.0	39.0
25-29	35.45544402568883	36.0	34.0	38.0	33.0	39.0
30-34	35.42398561909958	36.0	34.0	38.0	33.0	39.0
35-39	35.3988891082189	36.0	34.0	38.0	33.0	39.0
40-44	35.375687208721494	36.0	34.0	38.0	33.0	39.0
45-49	35.33882809641512	36.0	34.0	38.0	32.0	39.0
50-54	35.31293216458886	36.0	34.0	38.0	32.0	39.0
55-59	35.276554151529524	36.0	34.0	38.0	32.0	39.0
60-64	35.23282870960213	36.0	34.0	37.8	32.0	39.0
65-69	35.188821678078035	36.0	34.0	37.2	32.0	39.0
70-74	35.15296719962411	36.0	34.0	37.0	32.0	39.0
75-79	35.10138074884019	35.6	34.0	37.0	32.0	39.0
80-84	35.05555652590128	35.4	34.0	37.0	32.0	39.0
85-89	35.00377108710633	35.0	34.0	37.0	31.8	39.0
90-94	34.94040745507783	35.0	34.0	37.0	31.0	39.0
95-99	34.86896896815527	35.0	34.0	37.0	31.0	39.0
100-104	34.807892002925875	35.0	34.0	37.0	31.0	39.0
105-109	34.733075336323694	35.0	34.0	37.0	31.0	39.0
110-114	34.66030697343521	35.0	34.0	37.0	30.2	39.0
115-119	34.580346421539076	35.0	34.0	37.0	30.0	39.0
120-124	34.494360127003524	35.0	34.0	37.0	30.0	39.0
125-129	34.4208383778391	35.0	34.0	37.0	30.0	39.0
130-134	34.3165009512745	35.0	34.0	37.0	29.8	39.0
135-139	34.21144591419703	35.0	34.0	37.0	29.0	39.0
140-144	34.09979016623819	35.0	34.0	37.0	29.0	39.0
145-149	34.00233259714087	35.0	33.8	37.0	28.6	39.0
150	33.93176822991392	35.0	33.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	15.0
21	245.0
22	1903.0
23	9820.0
24	32392.0
25	78189.0
26	150202.0
27	252629.0
28	362765.0
29	470756.0
30	604176.0
31	784177.0
32	1058909.0
33	1508230.0
34	2339211.0
35	4134898.0
36	9897047.0
37	1318832.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.02351576424647	18.92198178800419	16.755309136974592	31.299193310774747
2	24.901300407691554	24.94800451444052	32.771172785590025	17.379522292277898
3	20.29108256285395	29.02765866409649	30.575897194628105	20.10536157842145
4	23.954866197547695	33.4178997677792	22.518327907631928	20.10890612704118
5	25.713518904491362	35.03069695229187	22.348158511060557	16.90762563215621
6	19.044421108813047	39.42370853298897	22.50530670196542	19.02656365623256
7	20.448687188160214	20.959019971874078	38.11521543203842	20.47707740792729
8	21.533141485976596	23.52399975656831	28.49543565578759	26.447423101667507
9	23.644175738372844	23.137872618374196	28.88140404090504	24.336547602347917
10-14	23.075467855977376	29.481365386707274	25.694569346241707	21.748597411073643
15-19	23.02999673845018	28.396919354692173	27.10422410042322	21.46885980643443
20-24	23.10869820083557	28.46884733056079	27.001994096694805	21.42046037190884
25-29	23.222304288644594	28.370357480727275	26.9140126708798	21.493325559748328
30-34	23.17585578516041	28.20550728532336	27.114404761071587	21.504232168444638
35-39	23.30670813815148	28.152832147205224	27.057371326469227	21.48308838817407
40-44	23.42118884121347	27.932006575611336	27.18872706140012	21.458077521775074
45-49	23.43573408788809	27.765702117309658	27.261227338727295	21.53733645607496
50-54	23.496427896495593	27.74389522672458	27.20715183511454	21.552525041665287
55-59	23.656089980347677	27.63040167835711	27.149289390321442	21.564218950973775
60-64	23.5441635711358	27.61091410291448	27.208822346645757	21.63609997930396
65-69	23.65534229732824	27.524261987956127	27.187715950526165	21.63267976418947
70-74	23.74101459977158	27.449391966345914	27.109332688123462	21.70026074575904
75-79	23.660720810087092	27.507088361172077	27.20121123260217	21.630979596138662
80-84	23.786939062546676	27.558760843707788	27.03354046935228	21.62075962439326
85-89	23.791832701925166	27.51472902945832	27.109636977724392	21.583801290892122
90-94	23.742498000643426	27.442831721137463	27.147554306217792	21.667115972001323
95-99	23.77991576689457	27.562667860826657	27.160677796265354	21.49673857601342
100-104	23.982963086063403	27.496267344670265	26.984859114566817	21.53591045469952
105-109	23.735659102795157	27.636782196589255	27.115467166478275	21.512091534137316
110-114	23.789262758895568	27.625534783539894	27.02682412813096	21.558378329433573
115-119	23.8406844311285	27.633502194936543	27.06478579524662	21.461027578688334
120-124	23.793248083268935	27.541881225249032	27.23148844431985	21.433382247162182
125-129	23.775246310477723	27.64264803069639	27.188241067437485	21.393864591388404
130-134	23.84485386078951	27.556043732675327	27.260821961849825	21.338280444685346
135-139	23.721650478967046	27.555630768216915	27.380610344950952	21.342108407865087
140-144	23.749122613538166	27.685751087368022	27.327434433089564	21.237691866004248
145-149	23.87117491127024	28.282023948374054	26.777004973961287	21.069796166394422
150	23.43406811592269	28.21528367550192	26.936915245043764	21.413732963531633
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	11.5
3	29.0
4	40.5
5	44.5
6	42.0
7	33.0
8	30.0
9	26.5
10	15.5
11	14.0
12	19.0
13	20.5
14	25.5
15	44.5
16	82.0
17	150.0
18	239.5
19	423.5
20	698.5
21	1094.5
22	1710.5
23	2804.5
24	4653.5
25	7555.5
26	11655.5
27	18111.5
28	29007.0
29	43455.0
30	58544.5
31	76620.5
32	102385.0
33	136969.0
34	181111.5
35	246042.5
36	342988.0
37	461244.0
38	600340.0
39	777303.0
40	985834.0
41	1179128.0
42	1347495.5
43	1502576.5
44	1614679.0
45	1663846.0
46	1656478.0
47	1600083.0
48	1499693.0
49	1357917.5
50	1180256.0
51	992196.0
52	810589.0
53	643513.5
54	500857.0
55	378828.5
56	279479.0
57	208081.0
58	153002.5
59	109090.0
60	75463.0
61	50096.5
62	33787.5
63	23001.0
64	15298.0
65	10429.0
66	7228.5
67	5350.0
68	4698.0
69	3571.5
70	2213.5
71	1538.0
72	821.0
73	566.5
74	486.5
75	320.0
76	212.5
77	76.5
78	28.0
79	3.5
80	0.5
81	0.5
82	0.5
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.304098289714216E-5
2	0.0
3	0.0
4	8.693988598094774E-6
5	0.0
6	0.0
7	0.0
8	4.346994299047387E-6
9	0.0
10-14	0.09520352214343682
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.693988598094773E-7
50-54	1.7387977196189546E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.7387977196189546E-6
80-84	8.693988598094773E-7
85-89	0.0
90-94	8.693988598094773E-7
95-99	6.955190878475818E-6
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.693988598094773E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3004401E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.444440149763324
#Duplication Level	Percentage of total
1	26.29667644573821
2	11.400848528408245
3	7.560911137480336
4	5.367989270154979
5	4.178340470821038
6	3.2351620945879267
7	2.6895251390457267
8	2.237245674639606
9	1.955920151609611
>10	21.916048857764938
>50	5.413245389357151
>100	6.003597249461187
>500	0.8643050245747843
>1k	0.7919910963037352
>5k	0.08819347005273204
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.346994299047386E-6	1.304098289714216E-5	0.0	4.346994299047386E-6	0.0015388359818627748	0.0
2	2.1734971495236934E-5	5.651092588761603E-5	0.0	4.346994299047386E-6	0.005625010622967318	0.0
3	5.216393158856864E-5	5.651092588761603E-5	0.0	4.346994299047386E-6	0.00718558157632533	0.0
4	1.0432786317713728E-4	5.651092588761603E-5	0.0	4.346994299047386E-6	0.00853314980903002	0.0
5	1.0867485747618467E-4	5.651092588761603E-5	0.0	4.346994299047386E-6	0.010232824579957549	0.0
6	1.1302185177523205E-4	5.651092588761603E-5	0.0	4.346994299047386E-6	0.011789048539016512	0.0
7	1.1736884607427944E-4	6.52049144857108E-5	0.0	4.346994299047386E-6	0.013719114007793552	0.0
8	1.4779780616761115E-4	6.955190878475818E-5	0.0	4.346994299047386E-6	0.019135468904406597	0.0
9	1.5649179476570591E-4	7.824589738285296E-5	0.0	4.346994299047386E-6	0.02501695219101771	0.0
10-11	1.7605326911141915E-4	9.128688027999512E-5	0.0	4.346994299047386E-6	0.03164394499991545	0.0
12-13	2.0648222920475087E-4	9.128688027999512E-5	0.0	4.346994299047386E-6	0.03734068102881705	2.173497149523693E-6
14-15	2.47778675045701E-4	9.346037742951881E-5	0.0	4.346994299047386E-6	0.0453348035447652	4.346994299047386E-6
16-17	2.7168714369046166E-4	9.99808688780899E-5	0.0	4.346994299047386E-6	0.05100545760787251	4.346994299047386E-6
18-19	3.0211610378379336E-4	1.0650136032666098E-4	0.0	4.346994299047386E-6	0.05874310746017686	4.346994299047386E-6
20-21	3.390655553256962E-4	1.1302185177523205E-4	0.0	4.346994299047386E-6	0.06463545823253558	4.346994299047386E-6
22-23	3.8036200116664634E-4	1.2823633182189792E-4	0.0	4.346994299047386E-6	0.07044956310751146	6.52049144857108E-6
24-25	4.2383194415712016E-4	1.3475682327046898E-4	0.0	4.346994299047386E-6	0.07560944534048072	8.693988598094772E-6
26-27	4.629548928485467E-4	1.3910381756951636E-4	8.693988598094772E-6	4.346994299047386E-6	0.07954564867826813	8.693988598094772E-6
28-29	5.477212816799707E-4	1.4127731471904006E-4	8.693988598094772E-6	4.346994299047386E-6	0.08362747632507363	8.693988598094772E-6
30-31	6.585696363056791E-4	1.4997130331713485E-4	1.304098289714216E-5	4.346994299047386E-6	0.08842003753977337	8.693988598094772E-6
32-33	7.781119795294822E-4	1.63012286214277E-4	1.7387977196189545E-5	4.346994299047386E-6	0.0932930181490055	8.693988598094772E-6
34-35	8.6505186551043E-4	1.7387977196189547E-4	1.7387977196189545E-5	4.346994299047386E-6	0.09630983219254438	8.693988598094772E-6
36-37	9.237362885475696E-4	1.7822676626094285E-4	1.7387977196189545E-5	4.346994299047386E-6	0.09992870494650133	8.693988598094772E-6
38-39	9.715532258370909E-4	1.8257376055999023E-4	1.7387977196189545E-5	4.346994299047386E-6	0.10322155312802972	8.693988598094772E-6
40-41	0.0010106761745285174	1.9344124630760873E-4	1.7387977196189545E-5	4.346994299047386E-6	0.10594059806208386	8.693988598094772E-6
42-43	0.001021543660276136	2.021352349057035E-4	1.7387977196189545E-5	4.346994299047386E-6	0.10865746949898847	8.693988598094772E-6
44-45	0.0010432786317713728	2.1300272065332196E-4	1.7387977196189545E-5	4.346994299047386E-6	0.11090269205444644	8.693988598094772E-6
46-47	0.0010889220719113705	2.4343168074665366E-4	1.7387977196189545E-5	4.346994299047386E-6	0.11349567415382822	1.5214480046665852E-5
48-49	0.0011367390092008917	2.47778675045701E-4	1.7387977196189545E-5	4.346994299047386E-6	0.1156952532691462	1.7387977196189545E-5
50-51	0.0011889029407894602	2.5647266364379584E-4	1.7387977196189545E-5	4.346994299047386E-6	0.1177057381324556	1.7387977196189545E-5
52-53	0.0012236788951818394	2.5647266364379584E-4	1.7387977196189545E-5	4.346994299047386E-6	0.1199074907449231	1.7387977196189545E-5
54-55	0.0012801898210694554	2.608196579428432E-4	1.7387977196189545E-5	4.346994299047386E-6	0.1218505971965973	1.7387977196189545E-5
56-57	0.0013301802555085005	2.651666522418906E-4	1.7387977196189545E-5	4.346994299047386E-6	0.12394584844873813	1.956147434571324E-5
58-59	0.0013779971927980216	2.7386064083998536E-4	1.7387977196189545E-5	4.346994299047386E-6	0.12584765845457135	2.1734971495236934E-5
60-61	0.0014366816158351613	2.955956123352223E-4	1.7387977196189545E-5	4.346994299047386E-6	0.12736910645923796	2.608196579428432E-5
62-63	0.0014844985531246827	3.0646309808284077E-4	1.7387977196189545E-5	4.346994299047386E-6	0.12930134542516453	3.477595439237909E-5
64-65	0.0015062335246199194	3.3471856102664877E-4	1.7387977196189545E-5	1.0867485747618465E-5	0.13111838904216633	4.129644584095017E-5
66-67	0.001623602370694199	3.608005268209331E-4	1.956147434571324E-5	2.173497149523693E-5	0.1324855187492167	5.216393158856864E-5
68-69	0.0016562048279370543	3.694945154190279E-4	2.1734971495236934E-5	2.608196579428432E-5	0.13385699545056617	6.52049144857108E-5
70-71	0.0016648988165351491	3.7384150971807523E-4	2.1734971495236934E-5	2.608196579428432E-5	0.13522195166046708	6.955190878475818E-5
72-73	0.0016822867937313388	3.999234755123596E-4	2.1734971495236934E-5	2.8255462943808013E-5	0.13614786144616414	6.955190878475818E-5
74-75	0.0017040217652265755	4.173114527085491E-4	2.1734971495236934E-5	3.26024572428554E-5	0.13726938597531838	6.955190878475818E-5
76-77	0.0017192362452732412	4.4339341850283345E-4	2.1734971495236934E-5	3.477595439237909E-5	0.13848437088190213	6.955190878475818E-5
78-79	0.0017800941654599048	4.825163671942599E-4	2.1734971495236934E-5	3.477595439237909E-5	0.13994061397208302	6.955190878475818E-5
80-81	0.0018040026341046656	4.933838529418784E-4	2.608196579428432E-5	3.477595439237909E-5	0.14142076553090863	6.955190878475818E-5
82-83	0.0018322580970484734	5.129453272875917E-4	2.8255462943808013E-5	3.477595439237909E-5	0.1430769703588457	6.955190878475818E-5
84-85	0.0018626870571418052	5.194658187361626E-4	3.0428960093331708E-5	3.694945154190279E-5	0.14471796070673607	7.172540593428187E-5
86-87	0.0018757280400389473	5.259863101847338E-4	3.26024572428554E-5	3.912294869142648E-5	0.14665237316981217	7.389890308380558E-5
88-89	0.0018996365086837081	5.259863101847338E-4	3.477595439237909E-5	3.912294869142648E-5	0.1483889973922816	7.389890308380558E-5
90-91	0.0019039835029827554	5.564152702780654E-4	3.477595439237909E-5	4.346994299047387E-5	0.14944749050409964	7.389890308380558E-5
92-93	0.0019039835029827554	5.564152702780654E-4	3.477595439237909E-5	4.346994299047387E-5	0.1504103497413386	7.389890308380558E-5
94-95	0.001906157000132279	5.651092588761603E-4	3.477595439237909E-5	4.346994299047387E-5	0.15149927181325	7.824589738285296E-5
96-97	0.00191267749158085	5.738032474742551E-4	3.477595439237909E-5	4.346994299047387E-5	0.1525903673823109	7.824589738285296E-5
98-99	0.00191267749158085	5.824972360723498E-4	3.477595439237909E-5	4.346994299047387E-5	0.15356844109959655	7.824589738285296E-5
100-101	0.00191267749158085	5.846707332218734E-4	3.477595439237909E-5	4.346994299047387E-5	0.15442262547935937	7.824589738285296E-5
102-103	0.00191267749158085	5.911912246704446E-4	3.912294869142648E-5	4.346994299047387E-5	0.15543764864818693	8.041939453237666E-5
104-105	0.0019213714801789448	5.911912246704446E-4	4.346994299047387E-5	4.346994299047387E-5	0.15643311034266877	8.476638883142404E-5
106-107	0.0019518004402722766	6.085792018666341E-4	4.346994299047387E-5	4.346994299047387E-5	0.15728512122528207	9.128688027999512E-5
108-109	0.0019518004402722766	6.237936819133E-4	4.346994299047387E-5	4.346994299047387E-5	0.15802628375326966	9.346037742951881E-5
110-111	0.001962667926019895	6.411816591094895E-4	5.216393158856864E-5	4.346994299047387E-5	0.1588761211387334	9.563387457904251E-5
112-113	0.001975708908917037	6.498756477075842E-4	5.216393158856864E-5	4.346994299047387E-5	0.15964119213536576	1.0215436602761359E-4
114-115	0.0019800559032160845	6.52049144857108E-4	5.216393158856864E-5	4.346994299047387E-5	0.16055840793246476	1.0432786317713728E-4
116-117	0.0019887498918141795	6.52049144857108E-4	5.216393158856864E-5	4.346994299047387E-5	0.16155821662124564	1.0867485747618466E-4
118-119	0.0019996173775617978	6.585696363056791E-4	5.651092588761603E-5	4.346994299047387E-5	0.1624232684867561	1.1302185177523205E-4
120-121	0.002003964371860845	6.607431334552028E-4	5.651092588761603E-5	4.346994299047387E-5	0.1633230963066589	1.1736884607427945E-4
122-123	0.0020104848633094165	6.629166306047265E-4	5.651092588761603E-5	4.346994299047387E-5	0.16418380117787026	1.2823633182189792E-4
124-125	0.0020213523490570347	6.694371220532975E-4	5.651092588761603E-5	4.346994299047387E-5	0.16517491587805308	1.304098289714216E-4
126-127	0.002043087320552272	6.80304607800916E-4	5.651092588761603E-5	4.346994299047387E-5	0.16614864260103968	1.3475682327046898E-4
128-129	0.0020648222920475086	6.998660821466293E-4	5.651092588761603E-5	4.346994299047387E-5	0.1671049813468301	1.3475682327046898E-4
130-131	0.0020800367720941746	7.259480479409135E-4	5.8684423037139725E-5	4.7816937289521256E-5	0.16823954685888148	1.4345081186856374E-4
132-133	0.0020909042578417933	7.346420365390083E-4	6.0857920186663416E-5	4.7816937289521256E-5	0.16910025173009285	1.5649179476570591E-4
134-135	0.0021235067150846484	7.411625279875794E-4	6.30314173361871E-5	4.7816937289521256E-5	0.16991313966401472	1.5866529191522962E-4
136-137	0.0021300272065332194	7.542035108847216E-4	6.52049144857108E-5	4.7816937289521256E-5	0.17067821066064706	1.6735928051332438E-4
138-139	0.0021430681894303617	7.628974994828163E-4	6.52049144857108E-5	4.7816937289521256E-5	0.17144545515442894	1.8692075485903762E-4
>>END_MODULE
