##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.R.3.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24076573
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.92339059217439	36.0	34.0	38.0	30.0	39.0
2	34.812499145954035	35.0	34.0	38.0	31.0	39.0
3	34.97389649266115	35.0	34.0	38.0	31.0	39.0
4	34.97206745328747	36.0	34.0	38.0	31.0	39.0
5	34.89081369678318	35.0	34.0	37.0	31.0	39.0
6	34.84498225723404	35.0	34.0	37.0	32.0	39.0
7	34.806541778184126	35.0	34.0	37.0	32.0	39.0
8	34.946065746150836	35.0	34.0	37.0	32.0	39.0
9	34.9213309967328	35.0	34.0	37.0	32.0	39.0
10-14	34.93751661417927	35.2	34.0	37.0	31.6	39.0
15-19	34.93626018952116	35.0	34.0	37.0	31.8	39.0
20-24	34.93288146946827	35.0	34.0	37.0	31.4	39.0
25-29	34.91996230526662	35.0	34.0	37.0	31.0	39.0
30-34	34.90302742836366	35.0	34.0	37.0	31.0	39.0
35-39	34.89604778055415	35.0	34.0	37.0	31.0	39.0
40-44	34.84076200545651	35.0	34.0	37.0	31.0	39.0
45-49	34.755471046481574	35.0	34.0	37.0	31.0	39.0
50-54	34.73627292389162	35.0	34.0	37.0	31.0	39.0
55-59	34.71686468834248	35.0	34.0	37.0	31.0	39.0
60-64	34.70793253674433	35.0	34.0	37.0	31.0	39.0
65-69	34.687473753012945	35.0	34.0	37.0	31.0	39.0
70-74	34.67687303338395	35.0	34.0	37.0	31.0	39.0
75-79	34.654851909364346	35.0	34.0	37.0	31.0	39.0
80-84	34.64575031504692	35.0	34.0	37.0	31.0	39.0
85-89	34.62784599785028	35.0	34.0	37.0	31.0	39.0
90-94	34.61496968027801	35.0	34.0	37.0	31.0	39.0
95-99	34.59525341916393	35.0	34.0	37.0	30.8	39.0
100-104	34.560200415565795	35.0	34.0	37.0	30.0	39.0
105-109	34.53710403885138	35.0	34.0	37.0	30.0	39.0
110-114	34.49881327379939	35.0	34.0	37.0	30.0	39.0
115-119	34.47606171360019	35.0	34.0	37.0	30.0	39.0
120-124	34.45282576552735	35.0	34.0	37.0	30.0	39.0
125-129	34.42586393836033	35.0	34.0	37.0	30.0	39.0
130-134	34.388943617515665	35.0	34.0	37.0	30.0	39.0
135-139	34.36331369086456	35.0	34.0	37.0	30.0	39.0
140-144	34.32459345439237	35.0	34.0	37.0	30.0	39.0
145-149	34.29709475679948	35.0	34.0	37.0	30.0	39.0
150	34.28307429799083	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	14.0
22	243.0
23	2720.0
24	19074.0
25	77128.0
26	196554.0
27	352316.0
28	510021.0
29	657916.0
30	818767.0
31	1035630.0
32	1343290.0
33	1831948.0
34	2671890.0
35	4473740.0
36	9012440.0
37	1072877.0
38	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.051456279992664	10.777717557734784	15.055242417331376	38.115583744941176
2	22.9872322228253	12.297223837902344	35.786587844735884	28.928956094536474
3	21.59845599198789	17.258072467913706	28.285973091088305	32.857498449010095
4	26.601644306170595	24.66246185394262	22.11081593209123	26.625077907795546
5	26.286123789075937	30.565868840599304	24.16879809736868	18.97920927295608
6	19.549114671487512	36.84889479357564	25.181090158998327	18.420900375938523
7	16.027923624047002	25.275252485501337	40.37313764179732	18.323686248654344
8	18.53640901745386	24.50793528696105	31.457709730995877	25.49794596458922
9	19.74537874931806	22.85516879602119	32.741338094813855	24.6581143598469
10-14	20.231121513200794	29.412154736684688	27.133733367173207	23.222990382941315
15-19	20.462909014606925	27.935503480485806	27.94393991425008	23.65764759065719
20-24	20.61703977769764	28.111499800191403	27.689905200588232	23.581555221522716
25-29	20.623933778956726	28.295518554483813	27.544167770737545	23.53637989582192
30-34	20.57230328905583	28.04872099241717	27.589013270623813	23.789962447903186
35-39	20.63850730821828	27.986498097483658	27.659401419875003	23.71559317442306
40-44	20.677305693008545	28.0623797946829	27.56920540905548	23.691109103253073
45-49	20.7733721544437	27.845814643064898	27.538542692527724	23.842270509963683
50-54	20.907246604732595	27.823684352311318	27.60657540538852	23.66249363756757
55-59	20.87830464195555	27.854376531377557	27.559676858252246	23.707641968414652
60-64	20.834838611902587	27.785653637075054	27.531134532604174	23.848373218418185
65-69	20.919274961827632	27.706003579049504	27.629811277562112	23.744910181560748
70-74	21.072396503341324	27.621754283356374	27.597261537387524	23.708587675914778
75-79	21.095336390780634	27.544710004063553	27.58927135675762	23.77068224839819
80-84	21.136364019611513	27.5345095850214	27.54992377675542	23.77920261861167
85-89	21.090601208647435	27.55700953211638	27.63007663073952	23.722312628496667
90-94	21.078167267262504	27.479205286134984	27.563980969742612	23.878646476859902
95-99	21.171425088473438	27.387310455223123	27.669297385597165	23.771967070706275
100-104	21.241734598875013	27.441086462554143	27.520222621263603	23.796956317307238
105-109	21.19477302284431	27.2891093228953	27.641530695626358	23.87458695863403
110-114	21.271974129601052	27.2617055131265	27.692295410944478	23.77402494632797
115-119	21.285562487343977	27.288133477082987	27.6787394718962	23.74756456367683
120-124	21.26110545608068	27.177877707036043	27.695874119371545	23.865142717511734
125-129	21.31001633020794	27.101020387336778	27.741240928503323	23.847722353951955
130-134	21.37492243473927	27.20772205262393	27.643938280065623	23.773417232571177
135-139	21.360302313031283	27.081108671064786	27.743179127232814	23.815409888671116
140-144	21.395017559938218	26.975151430179967	27.910435717497972	23.71939529238384
145-149	21.425042971999055	27.641428596479685	27.489316736928977	23.444211694592287
150	21.297828797432246	27.18266084903715	27.562184876497486	23.957325477033116
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	1.0
9	0.0
10	1.5
11	6.0
12	12.0
13	18.0
14	38.5
15	84.5
16	167.5
17	327.0
18	652.0
19	1279.5
20	2558.5
21	4307.5
22	6526.0
23	10118.5
24	15870.5
25	24518.5
26	35830.0
27	49900.0
28	67354.5
29	92960.0
30	125132.0
31	159003.0
32	193423.5
33	231524.5
34	272142.0
35	319267.0
36	391057.5
37	488140.5
38	606551.5
39	753181.0
40	921468.5
41	1088644.5
42	1255034.5
43	1426843.5
44	1567755.0
45	1650856.5
46	1670334.0
47	1628323.0
48	1541919.0
49	1413474.5
50	1246739.0
51	1069278.0
52	890335.5
53	715539.5
54	557575.0
55	421310.0
56	314736.0
57	234971.0
58	176064.5
59	130349.0
60	91845.5
61	64218.5
62	46374.5
63	31034.0
64	19762.5
65	14385.0
66	12358.0
67	9814.5
68	5568.5
69	2684.0
70	2247.5
71	1571.5
72	677.5
73	246.5
74	109.0
75	66.5
76	46.0
77	37.0
78	15.0
79	0.0
80	0.5
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.3259124128670636E-4
2	1.0383537557442248E-4
3	5.814781032167659E-5
4	1.4536952580419148E-4
5	9.137513050549179E-5
6	1.2460245068930697E-4
7	1.7444343096502979E-4
8	2.699719764934985E-4
9	1.1629562064335318E-4
10-14	1.7942752899260207E-4
15-19	2.0351733612586806E-4
20-24	1.2958654871687928E-4
25-29	2.8741631959000146E-4
30-34	4.2032560032526225E-4
35-39	5.972610803040781E-4
40-44	2.450514863556371E-4
45-49	2.1016280016263113E-4
50-54	3.713153030541348E-4
55-59	5.382825869778062E-4
60-64	7.800113413150618E-4
65-69	5.183461948675171E-4
70-74	2.3508329030049252E-4
75-79	4.344472114033837E-4
80-84	2.4588216936023244E-4
85-89	3.0319929667731367E-4
90-94	3.887596461506378E-4
95-99	5.731712731708122E-4
100-104	3.9374374417821005E-4
105-109	4.5853701853664977E-4
110-114	2.807708555532384E-4
115-119	3.5885505798520414E-4
120-124	3.081833947048859E-4
125-129	3.6716188803115796E-4
130-134	3.0319929667731367E-4
135-139	2.9904588165433673E-4
140-144	2.683106104843077E-4
145-149	3.355959338565335E-4
150	4.1534150229768994E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4076573E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.708327717432425
#Duplication Level	Percentage of total
1	36.551570826055034
2	11.204647470245984
3	6.747623931333665
4	4.946815313935321
5	3.484471282326276
6	2.8046056966392845
7	2.3416361095981126
8	1.9197188451050666
9	1.6971679247698515
>10	18.895390263601158
>50	4.5025182854569055
>100	4.28930344411708
>500	0.34635173372150896
>1k	0.26817887309477345
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.2460245068930699E-5	0.0	4.1534150229769E-6	1.370626957582377E-4	2.9073905160838295E-5
2	4.1534150229769E-6	1.1214220562037629E-4	0.0	1.66136600919076E-5	0.0016738262542596906	4.5687565252745894E-5
3	4.1534150229769E-6	1.24602450689307E-4	0.0	1.66136600919076E-5	0.0028367824606932222	8.306830045953799E-5
4	2.9073905160838295E-5	1.4121611078121458E-4	0.0	2.0767075114884496E-5	0.004203256003252622	1.0798879059739939E-4
5	3.32273201838152E-5	1.495229408271684E-4	0.0	2.0767075114884496E-5	0.0054658941702376	1.1214220562037629E-4
6	5.3994395298699696E-5	1.495229408271684E-4	0.0	2.0767075114884496E-5	0.006595623056487317	1.1629562064335318E-4
7	7.060805539060729E-5	1.8275026101098358E-4	0.0	2.0767075114884496E-5	0.007804266828173595	1.1629562064335318E-4
8	8.722171548251489E-5	1.8275026101098358E-4	0.0	2.0767075114884496E-5	0.009918355074868837	1.2875586571228388E-4
9	1.1214220562037629E-4	1.8275026101098358E-4	0.0	2.0767075114884496E-5	0.012991882191871742	1.329092807352608E-4
10-11	1.7236672345354135E-4	1.8898038354544892E-4	0.0	2.2843782626372947E-5	0.017612556404933543	1.7444343096502979E-4
12-13	2.2220770372926413E-4	1.910570910569374E-4	0.0	2.4920490137861398E-5	0.021402547613399964	2.1182416617182188E-4
14-15	2.3674465630968327E-4	2.0559404363735653E-4	0.0	2.4920490137861398E-5	0.025730406067341893	2.3882136382117174E-4
16-17	2.6997197649349845E-4	2.2428441124075257E-4	0.0	2.4920490137861398E-5	0.030149639651789316	2.533583164015909E-4
18-19	3.09429419211779E-4	2.49204901378614E-4	0.0	2.4920490137861398E-5	0.033896020002514474	2.533583164015909E-4
20-21	3.488868619300595E-4	2.762020990279638E-4	0.0	2.6997197649349848E-5	0.03758632925042946	2.595884389360562E-4
22-23	4.1326479478620147E-4	2.7827880653945227E-4	0.0	3.32273201838152E-5	0.04160891170018258	2.7412539151647534E-4
24-25	4.8179614266532036E-4	2.7827880653945227E-4	0.0	3.32273201838152E-5	0.046111213585089544	2.8243222156242915E-4
26-27	5.420206604984854E-4	2.7827880653945227E-4	0.0	3.73807352067921E-5	0.0501815603076069	2.948924666313599E-4
28-29	5.877082257512312E-4	2.8035551405094074E-4	0.0	3.73807352067921E-5	0.054808464643203166	3.0527600418880213E-4
30-31	6.458560360729079E-4	2.845089290739176E-4	0.0	3.73807352067921E-5	0.05951013044921302	3.2811978681517506E-4
32-33	7.081572614175615E-4	2.865856365854061E-4	0.0	4.361085774125744E-5	0.06357217034168441	3.634238145104787E-4
34-35	7.434612891128651E-4	2.865856365854061E-4	0.0	4.5687565252745894E-5	0.06725417275955345	3.7380735206792097E-4
36-37	7.600749492047726E-4	2.865856365854061E-4	0.0	4.776427276423435E-5	0.07088010407461229	3.821141821138748E-4
38-39	7.663050717392379E-4	2.9073905160838297E-4	0.0	4.9840980275722795E-5	0.07524326655624952	3.945744271828055E-4
40-41	8.07839221969007E-4	2.9489246663135985E-4	0.0	4.9840980275722795E-5	0.07877989944831434	3.945744271828055E-4
42-43	8.182227595264492E-4	3.0527600418880213E-4	0.0	4.9840980275722795E-5	0.08217946964462093	3.945744271828055E-4
44-45	8.472966646872875E-4	3.1565954174624436E-4	0.0	4.9840980275722795E-5	0.08643672004317227	4.0288125722875924E-4
46-47	9.012910599859871E-4	3.32273201838152E-4	0.0	4.9840980275722795E-5	0.0905091434732011	4.049579647402477E-4
48-49	9.303649651468255E-4	3.364266168611289E-4	0.0	4.9840980275722795E-5	0.09427213748401818	4.1118808727471306E-4
50-51	9.407485027042677E-4	3.385033243726173E-4	0.0	4.9840980275722795E-5	0.09857922886284523	4.2157162483215534E-4
52-53	9.947428980029674E-4	3.4058003188410576E-4	0.0	5.3994395298699696E-5	0.10281778889379314	4.2364833234364376E-4
54-55	0.0010404304632557135	3.4058003188410576E-4	0.0	5.814781032167659E-5	0.10657455278207575	4.2572503985513217E-4
56-57	0.0010736577834395285	3.426567393955942E-4	0.0	5.814781032167659E-5	0.11098132612145424	4.2780174736662064E-4
58-59	0.0010965015660659016	3.571936919760134E-4	0.0	6.64546403676304E-5	0.114981064788581	4.2780174736662064E-4
60-61	0.0011671096214565088	3.6550052202196715E-4	0.0	6.64546403676304E-5	0.11840347876751398	4.2780174736662064E-4
62-63	0.0011899534040828818	3.8626759713685166E-4	0.0	7.060805539060729E-5	0.12181550920888949	4.2780174736662064E-4
64-65	0.0012294108468011624	3.8626759713685166E-4	0.0	7.891488543656109E-5	0.12667915820079545	4.2780174736662064E-4
66-67	0.0012564080444505122	4.049579647402477E-4	0.0	8.306830045953799E-5	0.13106724117257051	4.298784548781091E-4
68-69	0.0013062490247262347	4.3403186990108604E-4	0.0	8.514500797102644E-5	0.13411377109192407	4.3610857741257445E-4
70-71	0.0013747803726053536	4.547989450159705E-4	0.0	8.722171548251489E-5	0.13686748525215778	4.4026199243555133E-4
72-73	0.001408007692789169	4.6102906755043585E-4	0.0	9.55285455284687E-5	0.1399389356616492	4.547989450159705E-4
74-75	0.0014682322106223341	4.776427276423434E-4	0.0	9.760525303995714E-5	0.1435171027039438	4.6518248257341273E-4
76-77	0.001491075993248707	4.901029727112741E-4	0.0	1.0383537557442248E-4	0.14621889917639025	4.6518248257341273E-4
78-79	0.0015346868509899642	4.942563877342511E-4	0.0	1.1837232815484164E-4	0.14886462454602656	4.6518248257341273E-4
80-81	0.0015596073411278258	4.963330952457395E-4	0.0	1.2044903566633009E-4	0.15184469982501247	4.6518248257341273E-4
82-83	0.0015907579538001525	5.025632177802048E-4	0.0	1.2044903566633009E-4	0.15453403605239002	4.6518248257341273E-4
84-85	0.0016551358866562945	5.025632177802048E-4	0.0	1.2044903566633009E-4	0.15703439189622212	4.6518248257341273E-4
86-87	0.0017361274796043442	5.067166328031818E-4	0.0	1.2044903566633009E-4	0.15921493478328497	4.6518248257341273E-4
88-89	0.0017901218749030437	5.150234628491355E-4	0.0	1.2044903566633009E-4	0.16136640376518702	4.6933589759638966E-4
90-91	0.0018088122425064398	5.254070004065779E-4	0.0	1.2044903566633009E-4	0.16402666608740374	4.797194351538319E-4
92-93	0.0018275026101098359	5.316371229410431E-4	0.0	1.2252574317781856E-4	0.16686552525560844	4.8594955768829724E-4
94-95	0.0018877271279430009	5.378672454755084E-4	0.0	1.24602450689307E-4	0.1697999129693416	4.942563877342511E-4
96-97	0.0019521050607991426	5.420206604984854E-4	0.0	1.24602450689307E-4	0.1722255073427601	4.942563877342511E-4
98-99	0.002008176163609331	5.482507830329508E-4	0.0	1.2667915820079544E-4	0.1741879959411167	4.963330952457395E-4
100-101	0.0020580171438850537	5.524041980559276E-4	0.0	1.2875586571228388E-4	0.1763643854131566	4.98409802757228E-4
102-103	0.002078784218999938	5.524041980559276E-4	0.0	1.2875586571228388E-4	0.17818358119322047	5.046399252916933E-4
104-105	0.0021140882466952416	5.524041980559276E-4	0.0	1.329092807352608E-4	0.17978472268457807	5.067166328031818E-4
106-107	0.0021722360570169182	5.58634320590393E-4	0.0	1.3498598824674925E-4	0.1814377818637229	5.067166328031818E-4
108-109	0.0022366139898730604	5.648644431248583E-4	0.0	1.370626957582377E-4	0.1831593723907468	5.108700478261587E-4
110-111	0.002276071432591341	5.794013957052775E-4	0.0	1.370626957582377E-4	0.18478958778726523	5.17100170360624E-4
112-113	0.0023321425354015293	5.835548107282544E-4	0.0	1.370626957582377E-4	0.1863408882983471	5.191768778721125E-4
114-115	0.002390290345723206	5.897849332627197E-4	0.0	1.3913940326972614E-4	0.1881102430981353	5.191768778721125E-4
116-117	0.0024255943734185093	5.980917633086735E-4	0.0	1.495229408271684E-4	0.1897944528899524	5.191768778721125E-4
118-119	0.0024816654762286976	6.022451783316504E-4	0.0	1.536763558501453E-4	0.19134990681605726	5.191768778721125E-4
120-121	0.0025647337766882355	6.063985933546273E-4	0.0	1.619831858960991E-4	0.1929946591651561	5.191768778721125E-4
122-123	0.002724640255072846	6.063985933546273E-4	0.0	1.619831858960991E-4	0.1944005901504338	5.191768778721125E-4
124-125	0.0028430125832276877	6.063985933546273E-4	0.0	1.619831858960991E-4	0.19595189066151564	5.212535853836009E-4
126-127	0.0028741631959000144	6.126287158890926E-4	0.0	1.619831858960991E-4	0.19749696105006306	5.295604154295547E-4
128-129	0.0029177740536412717	6.147054234005811E-4	0.0	1.7029001594205288E-4	0.19890081532782924	5.316371229410431E-4
130-131	0.0029759218639629484	6.167821309120696E-4	0.0	1.7029001594205288E-4	0.20029013265301504	5.399439529869969E-4
132-133	0.0030631435794454633	6.23012253446535E-4	0.0	1.7652013847651823E-4	0.2015029298397243	5.440973680099738E-4
134-135	0.003196052860180724	6.23012253446535E-4	0.0	1.785968459880067E-4	0.20260150811330166	5.440973680099738E-4
136-137	0.003328962140915985	6.333957910039772E-4	0.0	1.785968459880067E-4	0.2037104699244365	5.461740755214623E-4
138-139	0.0033871099512376617	6.520861586073732E-4	0.0	1.785968459880067E-4	0.2048422755181977	5.482507830329508E-4
>>END_MODULE
