##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24076573
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.988370562538115	37.0	35.0	38.0	33.0	39.0
2	35.47876086850068	35.0	34.0	38.0	33.0	39.0
3	35.6013770730577	36.0	35.0	37.0	33.0	39.0
4	35.71067248648718	36.0	35.0	38.0	33.0	39.0
5	35.63814185681658	36.0	35.0	38.0	33.0	39.0
6	35.57244234883428	36.0	35.0	37.0	33.0	39.0
7	35.52882389034353	35.0	34.0	38.0	33.0	39.0
8	35.69791813810047	36.0	35.0	38.0	33.0	39.0
9	35.674587990574906	36.0	35.0	38.0	33.0	39.0
10-14	35.592218211453925	36.0	35.0	37.8	33.0	39.0
15-19	35.60213176518103	36.0	35.0	38.0	33.0	39.0
20-24	35.582485389428136	36.0	35.0	38.0	33.0	39.0
25-29	35.564105688961625	36.0	35.0	38.0	33.0	39.0
30-34	35.537251617993974	36.0	35.0	38.0	33.0	39.0
35-39	35.516141578786986	36.0	35.0	38.0	33.0	39.0
40-44	35.49518092130471	36.0	35.0	38.0	33.0	39.0
45-49	35.45958108739147	36.0	34.0	38.0	33.0	39.0
50-54	35.438807076073495	36.0	34.0	38.0	33.0	39.0
55-59	35.40637020060953	36.0	34.0	38.0	33.0	39.0
60-64	35.367992579342584	36.0	34.0	38.0	32.4	39.0
65-69	35.32842501297838	36.0	34.0	38.0	32.0	39.0
70-74	35.29599903607544	36.0	34.0	38.0	32.0	39.0
75-79	35.248276372222904	36.0	34.0	38.0	32.0	39.0
80-84	35.208015127402064	36.0	34.0	38.0	32.0	39.0
85-89	35.16321225616287	36.0	34.0	38.0	32.0	39.0
90-94	35.10512652278213	36.0	34.0	37.4	32.0	39.0
95-99	35.038732688410434	35.8	34.0	37.0	32.0	39.0
100-104	34.98423047997736	35.4	34.0	37.0	31.6	39.0
105-109	34.917267860338754	35.0	34.0	37.0	31.0	39.0
110-114	34.85191443981667	35.0	34.0	37.0	31.0	39.0
115-119	34.77782971023326	35.0	34.0	37.0	31.0	39.0
120-124	34.697965287667806	35.0	34.0	37.0	30.6	39.0
125-129	34.63247576804224	35.0	34.0	37.0	30.0	39.0
130-134	34.53922506330116	35.0	34.0	37.0	30.0	39.0
135-139	34.44210449718072	35.0	34.0	37.0	30.0	39.0
140-144	34.338334762177325	35.0	34.0	37.0	29.8	39.0
145-149	34.24409051072177	35.0	34.0	37.0	29.0	39.0
150	34.173976213309096	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	16.0
21	222.0
22	1869.0
23	8985.0
24	30227.0
25	71821.0
26	140709.0
27	239444.0
28	345160.0
29	446814.0
30	568324.0
31	742413.0
32	1005308.0
33	1441185.0
34	2259016.0
35	4085131.0
36	1.099789E7
37	1692029.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.519237459425284	18.684565009431576	16.549970909475135	31.246226621668004
2	24.841966705226973	24.9062491122075	32.94412510219478	17.307659080370744
3	20.00180424348598	29.141281859341028	30.642641708186623	20.214272188986364
4	23.800918328444695	33.52977880446514	22.536743292888346	20.132559574201824
5	25.545278021525576	35.0917744889835	22.317609098072978	17.045338391417946
6	18.929828593130757	39.33615469277957	22.485949308483395	19.24806740560627
7	20.375798499229937	20.78994797141603	38.124400013241086	20.709853516112943
8	21.529401215031722	23.398168003394833	28.416847364448422	26.655583417125023
9	23.616895145334844	23.086425132015258	28.719793302809332	24.576886419840566
10-14	23.091096611084314	29.366133275173272	25.65025018660925	21.89251992713317
15-19	23.020274904078335	28.344021025650466	27.09192918929001	21.54377488098119
20-24	23.096301505970995	28.429826428035692	26.988627941171327	21.485244124821985
25-29	23.20390946003819	28.33464795841169	26.92649572677972	21.534946854770403
30-34	23.144519332456813	28.144297415488452	27.1360276586876	21.57515559336714
35-39	23.24543115002289	28.103862621976972	27.089607810879063	21.561098417121073
40-44	23.37479590637754	27.890422777361213	27.202699487173692	21.532081829087556
45-49	23.371801488291556	27.720499322893666	27.305514180157736	21.602185008657045
50-54	23.428408771341555	27.71557715582472	27.252886710734625	21.6031273620991
55-59	23.593802157807094	27.58858081671341	27.186219567045526	21.631397458433973
60-64	23.489783591539936	27.577825591338527	27.24940176908735	21.682989048034187
65-69	23.584786316304836	27.501632852842185	27.21850534490113	21.69507548595185
70-74	23.67326446334368	27.438750523174537	27.143274917073956	21.744710096407825
75-79	23.580838471682444	27.51614617300049	27.21803396913785	21.684981386179217
80-84	23.70149771730387	27.550319557521746	27.06340889959713	21.684773825577253
85-89	23.71351603901436	27.51848778478565	27.1350760758186	21.632920100381394
90-94	23.660217121931908	27.43997102444747	27.181523941812845	21.718287911807778
95-99	23.71163734237737	27.544756269487046	27.205192134710543	21.538414253425042
100-104	23.90373805866154	27.499563019194547	27.021318034455767	21.57538088768815
105-109	23.657536311334674	27.629651445826614	27.15393424138892	21.55887800144979
110-114	23.71080884310238	27.625974012165273	27.06180568139826	21.601411463334088
115-119	23.77962032868731	27.622089137204775	27.09791901943785	21.50037151467006
120-124	23.72661674067983	27.53649117754425	27.269656690759103	21.46723539101682
125-129	23.703709826145108	27.648186475708147	27.223526371464907	21.42457732668183
130-134	23.77345314052793	27.549537054131417	27.29214909447453	21.384860710866118
135-139	23.657891843660643	27.56202138900748	27.390861647959614	21.389225119372263
140-144	23.689129044147013	27.70370457376724	27.335523434631025	21.271642947454712
145-149	23.80916503357849	28.313286114265512	26.779841134367423	21.097707717788573
150	23.405984730468077	28.240858863094843	26.908875278886246	21.444281127550834
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	13.5
3	34.5
4	49.0
5	50.0
6	47.5
7	40.5
8	34.5
9	29.5
10	22.0
11	15.5
12	18.0
13	21.0
14	28.5
15	44.0
16	70.5
17	131.0
18	212.0
19	366.5
20	603.0
21	988.0
22	1587.5
23	2639.0
24	4389.5
25	7129.0
26	11018.0
27	17414.0
28	28414.0
29	43058.5
30	58703.5
31	77733.5
32	104608.0
33	140142.0
34	186724.5
35	256074.0
36	357501.0
37	480723.0
38	626915.5
39	813385.5
40	1031636.0
41	1234018.0
42	1412888.5
43	1576838.5
44	1692465.0
45	1743833.0
46	1737972.5
47	1678365.0
48	1571813.5
49	1423913.0
50	1239393.0
51	1039873.0
52	848352.5
53	675184.5
54	525664.5
55	397332.5
56	292538.5
57	216845.5
58	159223.5
59	113566.0
60	78243.0
61	52130.5
62	35383.0
63	24613.5
64	16625.0
65	10936.0
66	7406.5
67	5468.5
68	4651.0
69	3399.0
70	1831.5
71	1146.5
72	643.5
73	464.5
74	393.0
75	270.0
76	183.5
77	64.5
78	17.5
79	3.0
80	1.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.0767075114884496E-5
2	4.1534150229769E-6
3	0.0
4	8.3068300459538E-6
5	8.3068300459538E-6
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.10485628498707021
15-19	1.6613660091907598E-6
20-24	1.6613660091907598E-6
25-29	0.0
30-34	1.6613660091907598E-6
35-39	0.0
40-44	0.0
45-49	8.306830045953799E-7
50-54	2.4920490137861396E-6
55-59	0.0
60-64	1.6613660091907598E-6
65-69	1.6613660091907598E-6
70-74	0.0
75-79	3.3227320183815196E-6
80-84	0.0
85-89	0.0
90-94	8.306830045953799E-7
95-99	8.3068300459538E-6
100-104	2.4920490137861396E-6
105-109	0.0
110-114	0.0
115-119	8.306830045953799E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	8.306830045953799E-7
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4076573E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.96112095659247
#Duplication Level	Percentage of total
1	24.7895190033863
2	11.285294431032568
3	7.511709732919056
4	5.424189818029152
5	4.152593263478285
6	3.3604961824059956
7	2.7409677051970087
8	2.307660486799164
9	1.9829831319307174
>10	22.919361981225748
>50	5.672044771100944
>100	6.17498002507945
>500	0.8354655658279345
>1k	0.7546729832159489
>5k	0.08806091837181579
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.1534150229769E-6	4.1534150229769E-6	0.0	0.0	0.0016405989340758753	0.0
2	2.0767075114884496E-5	1.66136600919076E-5	0.0	0.0	0.005590496620926907	0.0
3	4.5687565252745894E-5	2.0767075114884496E-5	0.0	0.0	0.006886362108095699	0.0
4	9.55285455284687E-5	2.4920490137861398E-5	0.0	0.0	0.008161460520149608	0.0
5	9.968196055144559E-5	2.4920490137861398E-5	4.1534150229769E-6	0.0	0.009689917248605106	0.0
6	1.0383537557442248E-4	2.4920490137861398E-5	4.1534150229769E-6	0.0	0.01148003912350815	0.0
7	1.2875586571228388E-4	3.73807352067921E-5	4.1534150229769E-6	0.0	0.013407223694169431	0.0
8	1.7444343096502979E-4	4.153415022976899E-5	4.1534150229769E-6	0.0	0.018532537832522927	0.0
9	1.8275026101098358E-4	4.153415022976899E-5	4.1534150229769E-6	0.0	0.02450099522054073	0.0
10-11	2.2636111875224102E-4	5.3994395298699696E-5	4.1534150229769E-6	0.0	0.030600285181782306	0.0
12-13	2.6997197649349845E-4	6.437793285614195E-5	4.1534150229769E-6	0.0	0.03614924765247944	0.0
14-15	3.1565954174624436E-4	6.64546403676304E-5	4.1534150229769E-6	4.1534150229769E-6	0.044599370516725946	0.0
16-17	3.447334469070827E-4	6.64546403676304E-5	4.1534150229769E-6	8.3068300459538E-6	0.050368463983640865	0.0
18-19	3.9042101215982854E-4	6.853134787911883E-5	4.1534150229769E-6	8.3068300459538E-6	0.058172730811814455	0.0
20-21	4.2780174736662064E-4	7.268476290209574E-5	4.1534150229769E-6	1.038353755744225E-5	0.06408304038951058	0.0
22-23	4.8179614266532036E-4	7.47614704135842E-5	4.1534150229769E-6	1.2460245068930699E-5	0.06991858849679312	0.0
24-25	5.295604154295548E-4	8.929842299400335E-5	4.1534150229769E-6	1.2460245068930699E-5	0.07505843958772704	2.07670751148845E-6
26-27	5.710945656593237E-4	9.55285455284687E-5	4.1534150229769E-6	1.2460245068930699E-5	0.07887335128633133	4.1534150229769E-6
28-29	6.915436013256537E-4	9.968196055144559E-5	4.1534150229769E-6	1.2460245068930699E-5	0.08275471762530323	4.1534150229769E-6
30-31	8.576802022447297E-4	1.0383537557442248E-4	8.3068300459538E-6	1.2460245068930699E-5	0.08755398868435305	4.1534150229769E-6
32-33	0.0010362770482327363	1.1421891313186474E-4	8.3068300459538E-6	1.2460245068930699E-5	0.09231795571570756	4.1534150229769E-6
34-35	0.0011899534040828818	1.2667915820079544E-4	8.3068300459538E-6	1.2460245068930699E-5	0.09535617880501515	8.3068300459538E-6
36-37	0.0012564080444505122	1.4121611078121458E-4	8.3068300459538E-6	1.2460245068930699E-5	0.09865606704077029	8.3068300459538E-6
38-39	0.0012854819496113503	1.4536952580419148E-4	8.3068300459538E-6	1.66136600919076E-5	0.10208678784974921	8.3068300459538E-6
40-41	0.0013124791472607002	1.5575306336163374E-4	8.3068300459538E-6	1.66136600919076E-5	0.10473458992689699	1.038353755744225E-5
42-43	0.0013394763449100502	1.6821330843056444E-4	8.3068300459538E-6	1.66136600919076E-5	0.10747792054957322	1.2460245068930699E-5
44-45	0.0013498598824674924	1.8067355349949513E-4	8.3068300459538E-6	1.66136600919076E-5	0.10968961404930844	1.2460245068930699E-5
46-47	0.0013747803726053536	2.2428441124075257E-4	8.3068300459538E-6	1.8690367603396046E-5	0.1120861345175661	1.2460245068930699E-5
48-49	0.0014121611078121458	2.2843782626372948E-4	8.3068300459538E-6	2.0767075114884496E-5	0.1146384080491854	1.2460245068930699E-5
50-51	0.0014723856256453108	2.3259124128670636E-4	8.3068300459538E-6	2.0767075114884496E-5	0.1168147975212253	1.2460245068930699E-5
52-53	0.0015035362383176375	2.3674465630968327E-4	8.3068300459538E-6	2.0767075114884496E-5	0.1190451813885639	1.2460245068930699E-5
54-55	0.0015346868509899644	2.6166514644754464E-4	8.3068300459538E-6	2.0767075114884496E-5	0.1209869029118056	1.2460245068930699E-5
56-57	0.0015575306336163375	2.720486840049869E-4	8.3068300459538E-6	2.0767075114884496E-5	0.12308645420592042	1.2460245068930699E-5
58-59	0.0016156784439380141	2.845089290739176E-4	8.3068300459538E-6	2.0767075114884496E-5	0.12513201110473654	1.2460245068930699E-5
60-61	0.0017049768669320173	3.0527600418880213E-4	8.3068300459538E-6	2.0767075114884496E-5	0.12657324611770954	1.66136600919076E-5
62-63	0.0017465110171617864	3.1565954174624436E-4	8.3068300459538E-6	2.0767075114884496E-5	0.12851289093343973	1.8690367603396046E-5
64-65	0.0017672780922766708	3.2811978681517506E-4	8.3068300459538E-6	2.2843782626372947E-5	0.1303009361008313	2.4920490137861398E-5
66-67	0.0018171190725523936	3.4681015441857116E-4	8.3068300459538E-6	2.4920490137861398E-5	0.1317047903785975	2.4920490137861398E-5
68-69	0.0018441162702017434	3.571936919760134E-4	8.3068300459538E-6	2.4920490137861398E-5	0.13302349964839266	2.4920490137861398E-5
70-71	0.0018544998077591856	3.6550052202196715E-4	8.3068300459538E-6	2.4920490137861398E-5	0.13444604429376222	2.9073905160838295E-5
72-73	0.0018960339579889548	3.758840595794094E-4	8.3068300459538E-6	2.4920490137861398E-5	0.13542832694669626	2.9073905160838295E-5
74-75	0.0019043407880349086	3.9042101215982854E-4	8.3068300459538E-6	2.4920490137861398E-5	0.13654144217285408	2.9073905160838295E-5
76-77	0.0019562584758221198	4.070346722517362E-4	2.0767075114884496E-5	2.4920490137861398E-5	0.13789545547034454	2.9073905160838295E-5
78-79	0.002008176163609331	4.3818528492406287E-4	2.9073905160838295E-5	2.4920490137861398E-5	0.13940106841617367	2.9073905160838295E-5
80-81	0.002012329578632308	4.4856882248150515E-4	2.9073905160838295E-5	2.4920490137861398E-5	0.14068032024325056	2.9073905160838295E-5
82-83	0.0020310199462357037	4.672591900849012E-4	3.32273201838152E-5	2.4920490137861398E-5	0.14210494159613163	2.9073905160838295E-5
84-85	0.0020476336063276115	4.6933589759638966E-4	3.32273201838152E-5	2.4920490137861398E-5	0.14368739271988584	3.32273201838152E-5
86-87	0.002072554096465473	4.7348931261936654E-4	3.32273201838152E-5	2.4920490137861398E-5	0.1454878981323463	3.32273201838152E-5
88-89	0.0020850143415344037	4.8179614266532036E-4	3.32273201838152E-5	2.6997197649349848E-5	0.14719495170678984	3.530402769530365E-5
90-91	0.0020974745866033343	4.98409802757228E-4	3.32273201838152E-5	3.115061267232674E-5	0.1480962427667758	3.73807352067921E-5
92-93	0.0021078581241607765	5.025632177802048E-4	3.32273201838152E-5	3.73807352067921E-5	0.1490016872417848	3.73807352067921E-5
94-95	0.0021161649542067304	5.108700478261587E-4	3.32273201838152E-5	3.73807352067921E-5	0.15004627112006347	3.73807352067921E-5
96-97	0.0021182416617182187	5.274837079180662E-4	3.32273201838152E-5	3.73807352067921E-5	0.15099532645281372	4.1534150229769E-5
98-99	0.0021182416617182187	5.274837079180662E-4	3.530402769530365E-5	3.73807352067921E-5	0.15195268861560987	5.3994395298699696E-5
100-101	0.0021265484917641726	5.295604154295547E-4	3.73807352067921E-5	3.73807352067921E-5	0.15283944272301545	5.814781032167659E-5
102-103	0.002128625199275661	5.420206604984854E-4	4.153415022976899E-5	3.73807352067921E-5	0.15379472817830014	6.23012253446535E-5
104-105	0.0021390087368331036	5.461740755214623E-4	4.153415022976899E-5	3.73807352067921E-5	0.15469186582326314	6.64546403676304E-5
106-107	0.002155622396925011	5.731712731708121E-4	4.153415022976899E-5	3.73807352067921E-5	0.15553085565790448	7.060805539060729E-5
108-109	0.002159775811947988	6.063985933546273E-4	4.5687565252745894E-5	3.73807352067921E-5	0.15633454146485048	7.060805539060729E-5
110-111	0.0021722360570169187	6.250889609580234E-4	4.9840980275722795E-5	3.73807352067921E-5	0.15733343777787645	7.268476290209574E-5
112-113	0.0021763894720398954	6.458560360729079E-4	5.191768778721124E-5	3.73807352067921E-5	0.15815166053740287	7.891488543656109E-5
114-115	0.0021826195945743605	6.500094510958848E-4	5.3994395298699696E-5	3.73807352067921E-5	0.15894911622181446	7.891488543656109E-5
116-117	0.0021909264246203148	6.541628661188617E-4	5.3994395298699696E-5	3.73807352067921E-5	0.15997500973248976	8.099159294804953E-5
118-119	0.00219715654715478	6.624696961648154E-4	5.814781032167659E-5	3.73807352067921E-5	0.1608119228596196	8.722171548251489E-5
120-121	0.00219715654715478	6.645464036763039E-4	5.814781032167659E-5	3.9457442718280545E-5	0.16164883598674945	8.722171548251489E-5
122-123	0.00219715654715478	6.686998186992808E-4	5.814781032167659E-5	4.153415022976899E-5	0.16242552459604614	9.137513050549179E-5
124-125	0.002201309962177757	6.707765262107693E-4	5.814781032167659E-5	4.153415022976899E-5	0.16331643211847466	9.55285455284687E-5
126-127	0.0022096167922237104	6.770066487452346E-4	5.814781032167659E-5	4.153415022976899E-5	0.16432155855403507	9.968196055144559E-5
128-129	0.0022220770372926415	6.811600637682115E-4	5.814781032167659E-5	4.153415022976899E-5	0.16524153998162444	1.0798879059739939E-4
130-131	0.002234537282361572	6.915436013256537E-4	6.0224517833165045E-5	4.153415022976899E-5	0.16631519776506398	1.0798879059739939E-4
132-133	0.0022553043574764565	6.977737238601191E-4	6.64546403676304E-5	4.153415022976899E-5	0.16714380406214788	1.24602450689307E-4
134-135	0.002269841310056876	7.102339689290498E-4	7.060805539060729E-5	4.153415022976899E-5	0.16800563767941556	1.24602450689307E-4
136-137	0.0022781481401028293	7.185407989750036E-4	7.268476290209574E-5	4.153415022976899E-5	0.16897961350230367	1.329092807352608E-4
138-139	0.0022802248476143177	7.185407989750036E-4	7.47614704135842E-5	4.153415022976899E-5	0.16984767724210584	1.4536952580419148E-4
>>END_MODULE
