##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.S.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24210574
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.896343928070436	37.0	35.0	38.0	33.0	39.0
2	35.50965987010469	35.0	34.0	38.0	33.0	39.0
3	35.59268037180779	35.0	35.0	38.0	33.0	39.0
4	35.68204995883204	36.0	35.0	38.0	33.0	39.0
5	35.605112873408125	36.0	35.0	38.0	33.0	39.0
6	35.545128174160595	35.0	35.0	37.0	33.0	39.0
7	35.56477549850739	35.0	34.0	38.0	33.0	39.0
8	35.69177963314707	36.0	35.0	38.0	33.0	39.0
9	35.67658474350918	36.0	35.0	38.0	33.0	39.0
10-14	35.64930180507079	35.8	35.0	37.8	33.0	39.0
15-19	35.62899754462657	36.0	35.0	38.0	33.0	39.0
20-24	35.61986375870312	36.0	35.0	38.0	33.0	39.0
25-29	35.604478778570055	36.0	35.0	38.0	33.0	39.0
30-34	35.58883584503201	36.0	35.0	38.0	33.0	39.0
35-39	35.5637096253893	36.0	35.0	38.0	33.0	39.0
40-44	35.54986470787516	36.0	35.0	38.0	33.0	39.0
45-49	35.52169306683931	36.0	35.0	38.0	33.0	39.0
50-54	35.50466732428566	36.0	35.0	38.0	33.0	39.0
55-59	35.47949540560253	36.0	35.0	38.0	33.0	39.0
60-64	35.44050739978325	36.0	34.2	38.0	33.0	39.0
65-69	35.417487887730374	36.0	34.0	38.0	33.0	39.0
70-74	35.387941838966725	36.0	34.0	38.0	32.8	39.0
75-79	35.34072757630612	36.0	34.0	38.0	32.0	39.0
80-84	35.30431935236233	36.0	34.0	38.0	32.0	39.0
85-89	35.25643166494111	36.0	34.0	38.0	32.0	39.0
90-94	35.20780483767134	36.0	34.0	38.0	32.0	39.0
95-99	35.14490525503444	36.0	34.0	38.0	32.0	39.0
100-104	35.10007535550376	36.0	34.0	38.0	32.0	39.0
105-109	35.04520760226503	36.0	34.0	38.0	32.0	39.0
110-114	34.97347371441917	35.4	34.0	38.0	31.0	39.0
115-119	34.90648567853038	35.0	34.0	37.6	31.0	39.0
120-124	34.835211292388195	35.0	34.0	37.0	31.0	39.0
125-129	34.76135396872458	35.0	34.0	37.0	31.0	39.0
130-134	34.66168783111049	35.0	34.0	37.0	30.2	39.0
135-139	34.608237871601055	35.0	34.0	37.0	30.0	39.0
140-144	34.49053814254879	35.0	34.0	37.0	30.0	39.0
145-149	34.41890816797652	35.0	34.0	37.0	30.0	39.0
150	34.35589808816594	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	212.0
22	1617.0
23	8183.0
24	26754.0
25	63725.0
26	124486.0
27	214358.0
28	320794.0
29	428737.0
30	561614.0
31	744281.0
32	1012966.0
33	1435005.0
34	2187356.0
35	3800806.0
36	1.0870029E7
37	2409634.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.04772534513226	19.14546511784479	16.276185769077596	31.53062376794536
2	24.892747276458625	25.2532261316894	31.938966007166954	17.915060584685023
3	20.346816229966294	29.113299833370327	30.24124913353975	20.298634803123626
4	23.68937225527986	33.70971708477461	21.945799385012517	20.65511127493301
5	25.92929849577296	34.87999499722724	21.78980969224439	17.400896814755402
6	19.307315886025666	39.46035314982619	21.74496978055952	19.487361183588625
7	20.697142496497605	20.95413764250282	37.28558438969683	21.063135471302747
8	21.636323038024624	23.551147527522478	28.253382179208142	26.559147255244753
9	23.866171037497914	23.126151408058313	28.01482938818386	24.992848166259915
10-14	23.14082764002208	29.03945276142565	25.385696349041538	22.43402324951073
15-19	23.25885705972936	27.804166063968577	26.821502042867717	22.11547483343435
20-24	23.10337540943887	27.922174831542613	26.736356601871563	22.238093157146956
25-29	23.265730915756066	27.49679871282688	26.718288463544898	22.519181907872156
30-34	23.081569234996245	27.24586620705482	26.952295307000984	22.720269250947954
35-39	23.393145490891705	26.9758659997074	26.9336860827835	22.697302426617394
40-44	23.530616002743265	26.66012957809261	27.039606743731063	22.769647675433056
45-49	23.43747177111515	26.496661553721623	27.150580792926103	22.915285882237125
50-54	23.608291980190145	26.48559840010402	26.91604255231619	22.990067067389646
55-59	23.70077801542417	26.45447480923005	26.951738525488906	22.893008649856878
60-64	23.44198530774198	26.42002870316086	27.030081979882013	23.107904009215147
65-69	23.601715904822676	26.381382389056657	26.95941908621281	23.05748261990786
70-74	23.796394087971645	26.314642519421472	26.755436694726857	23.133526697880026
75-79	23.640839742172158	26.43170211495192	26.96392163192826	22.963536510947655
80-84	23.934670859104788	26.435268325319345	26.635111583888925	22.99494923168695
85-89	23.746361403905585	26.52388745512601	26.824630428010504	22.9051207129579
90-94	23.750445569774595	26.32213924378662	26.813386580590777	23.114028605848006
95-99	23.7391647446208	26.536220302221835	26.850131077851614	22.874483875305756
100-104	23.97737368804226	26.280877933749114	26.826147120675454	22.915601257533176
105-109	23.772820090923908	26.47928215167472	26.792216491851867	22.955681265549508
110-114	23.862499088208317	26.582700600159253	26.689039260283543	22.865761051348883
115-119	23.970094224118768	26.591714843274676	26.834515365063215	22.603675567543338
120-124	23.86679721017767	26.677935021284497	27.023362601811918	22.43190516672591
125-129	23.905022854105177	26.767845543586414	26.833326023854916	22.493805578453493
130-134	24.074978561337602	26.752505786500663	26.803221817244456	22.369293834917283
135-139	23.85985891949526	26.82084943545742	27.005622419361057	22.313669225686265
140-144	23.885905389934166	26.93946950617528	26.850742985275772	22.323882118614787
145-149	23.99252181292787	27.614859751898983	26.282633582191266	22.10998485298188
150	23.827373939998285	27.558999633796372	26.22954333920377	22.38408308700157
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	0.5
9	2.5
10	5.0
11	8.0
12	14.5
13	15.0
14	25.0
15	51.5
16	71.0
17	120.5
18	224.0
19	382.5
20	635.5
21	1035.0
22	1538.0
23	2344.0
24	3889.0
25	6074.5
26	9052.0
27	13869.5
28	20330.0
29	28254.5
30	39807.0
31	56798.5
32	82916.0
33	112648.5
34	147384.0
35	205131.0
36	290721.5
37	389242.5
38	505580.0
39	659131.0
40	843485.5
41	1034250.0
42	1224080.0
43	1406612.5
44	1546360.5
45	1627809.0
46	1648483.5
47	1607512.5
48	1545200.5
49	1478253.0
50	1360300.5
51	1195458.0
52	1017198.5
53	843650.5
54	695085.0
55	578208.5
56	473321.0
57	386797.0
58	323805.0
59	263866.0
60	199005.5
61	134340.5
62	83254.0
63	50963.0
64	31298.0
65	15467.5
66	8073.0
67	4240.5
68	2301.0
69	1382.0
70	922.0
71	791.5
72	496.0
73	343.5
74	291.0
75	190.0
76	120.5
77	37.5
78	9.5
79	3.0
80	2.0
81	0.0
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.260853294928075E-7
50-54	0.0
55-59	0.0
60-64	0.0
65-69	1.652170658985615E-6
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	8.260853294928075E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	8.260853294928075E-7
130-134	0.052466331446747186
135-139	0.0
140-144	0.0
145-149	8.260853294928075E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4210574E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.42224856237257
#Duplication Level	Percentage of total
1	25.428292704887472
2	8.775783146522622
3	5.533024760728244
4	3.9827334385398983
5	2.9994061389622644
6	2.411688172666245
7	1.9459121110474276
8	1.6760172759815624
9	1.4553187807631924
>10	17.830512734857635
>50	6.274556988840713
>100	12.662200307852794
>500	3.7606041062132376
>1k	4.43763816399265
>5k	0.5710505694929782
>10k+	0.2552605986511706
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.932602919699467E-4	8.260853294928076E-6	0.0	4.130426647464038E-6	0.0018132572982367126	0.0
2	0.0016108663925109748	2.065213323732019E-5	0.0	4.130426647464038E-6	0.009537155128994463	0.0
3	0.002399777882176606	2.065213323732019E-5	0.0	4.130426647464038E-6	0.013138887165583105	0.0
4	0.005898249252578646	2.065213323732019E-5	0.0	4.130426647464038E-6	0.01869018057977477	0.0
5	0.00609650973165692	2.065213323732019E-5	0.0	4.130426647464038E-6	0.022267130056478627	0.0
6	0.007112594686933073	2.065213323732019E-5	0.0	4.130426647464038E-6	0.02925581194398778	0.0
7	0.00922737313043466	2.4782559884784227E-5	0.0	4.130426647464038E-6	0.03875579323315507	0.0
8	0.015778229793312625	2.4782559884784227E-5	0.0	4.130426647464038E-6	0.054137502068311145	0.0
9	0.016393663363784765	2.4782559884784227E-5	0.0	4.130426647464038E-6	0.06557465345513906	0.0
10-11	0.01700909693425691	4.130426647464038E-5	0.0	4.130426647464038E-6	0.11203162717249082	0.0
12-13	0.01765550870458503	4.130426647464038E-5	0.0	4.130426647464038E-6	0.13427810509573213	0.0
14-15	0.020319633892199332	4.130426647464038E-5	0.0	4.130426647464038E-6	0.14425308544935778	0.0
16-17	0.023336910558171817	4.130426647464038E-5	0.0	4.130426647464038E-6	0.15081633339217815	0.0
18-19	0.02963374598223074	4.7499906445836434E-5	0.0	4.130426647464038E-6	0.15784012390619073	0.0
20-21	0.03530895219584633	4.956511976956845E-5	0.0	4.130426647464038E-6	0.16307957010849888	0.0
22-23	0.03747536097244122	5.369554641703249E-5	0.0	4.130426647464038E-6	0.1682219512845916	0.0
24-25	0.042194373417168876	6.815203968315662E-5	0.0	4.130426647464038E-6	0.173228028381318	0.0
26-27	0.04644251722408564	7.021725300688864E-5	0.0	4.130426647464038E-6	0.17728617256245144	0.0
28-29	0.05690488792211205	7.021725300688864E-5	2.065213323732019E-6	4.130426647464038E-6	0.18084040469259424	0.0
30-31	0.07904810517916674	7.434767965435268E-5	4.130426647464038E-6	4.130426647464038E-6	0.18465898412817472	0.0
32-33	0.09755448177312938	9.499981289167287E-5	4.130426647464038E-6	4.130426647464038E-6	0.18794267331290865	0.0
34-35	0.11206467058567054	9.499981289167287E-5	4.130426647464038E-6	6.195639971196057E-6	0.19103016723188804	0.0
36-37	0.11462553510709825	9.91302395391369E-5	4.130426647464038E-6	8.260853294928076E-6	0.1930788588490302	0.0
38-39	0.116661835444298	1.0119545286286892E-4	4.130426647464038E-6	8.260853294928076E-6	0.19485494230743972	0.0
40-41	0.11807650657105445	1.0532587951033297E-4	4.130426647464038E-6	8.260853294928076E-6	0.19648852604651174	0.0
42-43	0.11834911472978707	1.1152151948152902E-4	4.130426647464038E-6	8.260853294928076E-6	0.19799200134618866	2.065213323732019E-6
44-45	0.11902443948664745	1.197823727764571E-4	4.130426647464038E-6	8.260853294928076E-6	0.19927036839357878	4.130426647464038E-6
46-47	0.12044324104005133	1.2391279942392113E-4	4.130426647464038E-6	8.260853294928076E-6	0.20066645260042162	4.130426647464038E-6
48-49	0.1215625866615141	1.321736527188492E-4	4.130426647464038E-6	8.260853294928076E-6	0.20191797187460322	6.195639971196057E-6
50-51	0.12246715009730871	1.3423886604258125E-4	4.130426647464038E-6	8.260853294928076E-6	0.2033491647079495	8.260853294928076E-6
52-53	0.1233758439597508	1.3836929269004527E-4	4.130426647464038E-6	8.260853294928076E-6	0.2044974233159445	1.4456493266124132E-5
54-55	0.12386736473079904	1.4043450601377728E-4	4.130426647464038E-6	8.260853294928076E-6	0.20557752988425637	1.652170658985615E-5
56-57	0.12470584134023423	1.4043450601377728E-4	4.130426647464038E-6	8.260853294928076E-6	0.20680220138522945	1.652170658985615E-5
58-59	0.12485453669954294	1.4663014598497335E-4	6.195639971196057E-6	8.260853294928076E-6	0.20769850396772915	1.652170658985615E-5
60-61	0.1251498622048366	1.507605726324374E-4	8.260853294928076E-6	8.260853294928076E-6	0.20857002399034405	2.065213323732019E-5
62-63	0.12539975301700818	1.528257859561694E-4	8.260853294928076E-6	8.260853294928076E-6	0.2096191523587999	2.065213323732019E-5
64-65	0.12542247036356924	1.6108663925109749E-4	8.260853294928076E-6	8.260853294928076E-6	0.21073643276693893	2.4782559884784227E-5
66-67	0.1254720354833388	1.858691991358817E-4	8.260853294928076E-6	8.260853294928076E-6	0.21160175714958265	2.4782559884784227E-5
68-69	0.12551127453648972	1.858691991358817E-4	8.260853294928076E-6	1.0326066618660094E-5	0.21238240778595335	2.4782559884784227E-5
70-71	0.12559181785611526	1.858691991358817E-4	8.260853294928076E-6	1.652170658985615E-5	0.21307425424940357	2.4782559884784227E-5
72-73	0.1256517090425035	1.8793441245961373E-4	8.260853294928076E-6	1.652170658985615E-5	0.2136814269665808	2.4782559884784227E-5
74-75	0.12568888288233065	1.8999962578334574E-4	8.260853294928076E-6	1.652170658985615E-5	0.2144868601628363	2.8912986532248264E-5
76-77	0.12571779586886292	1.941300524308098E-4	1.2391279942392113E-5	1.652170658985615E-5	0.21527577165250195	2.8912986532248264E-5
78-79	0.1257673609886325	2.065213323732019E-4	1.4456493266124132E-5	1.652170658985615E-5	0.21628566096780688	3.30434131797123E-5
80-81	0.125794208761841	2.065213323732019E-4	2.065213323732019E-5	1.652170658985615E-5	0.21720674611019136	3.30434131797123E-5
82-83	0.1258334478149919	2.065213323732019E-4	2.4782559884784227E-5	1.652170658985615E-5	0.21806380963954014	3.717383982717634E-5
84-85	0.12586236080152416	2.085865456969339E-4	2.4782559884784227E-5	1.858691991358817E-5	0.21893119923550758	3.717383982717634E-5
86-87	0.12588094772143776	2.16847398991862E-4	2.4782559884784227E-5	2.065213323732019E-5	0.21974695849838174	3.923905315090836E-5
88-89	0.12590779549464626	2.1891261231559402E-4	2.4782559884784227E-5	2.065213323732019E-5	0.22061228288102547	4.130426647464038E-5
90-91	0.12594496933447344	2.2304303896305804E-4	2.4782559884784227E-5	2.065213323732019E-5	0.2212648902913248	4.130426647464038E-5
92-93	0.12595116497444464	2.2304303896305804E-4	2.4782559884784227E-5	2.065213323732019E-5	0.2218927151417393	4.130426647464038E-5
94-95	0.1259738823210057	2.2304303896305804E-4	2.4782559884784227E-5	2.065213323732019E-5	0.22262586587166416	4.3369479798372397E-5
96-97	0.1259883388142718	2.2304303896305804E-4	2.4782559884784227E-5	2.065213323732019E-5	0.2233507557482941	4.5434693122104415E-5
98-99	0.1260007300942142	2.271734656105221E-4	2.4782559884784227E-5	2.2717346561052208E-5	0.22400542837191717	5.163033309330047E-5
100-101	0.126025512654099	2.271734656105221E-4	2.6847773208516245E-5	2.4782559884784227E-5	0.2245361881961163	5.369554641703249E-5
102-103	0.12603996914736512	2.3130389225798611E-4	3.30434131797123E-5	2.4782559884784227E-5	0.22519292603306307	5.369554641703249E-5
104-105	0.12605442564063124	2.3130389225798611E-4	3.717383982717634E-5	2.6847773208516245E-5	0.22596325060281514	5.782597306449653E-5
106-107	0.12607507777386856	2.3749953222918218E-4	3.717383982717634E-5	2.8912986532248264E-5	0.22661379279979071	6.195639971196057E-5
108-109	0.12609779512042962	2.395647455529142E-4	3.717383982717634E-5	3.30434131797123E-5	0.22719618295708313	6.195639971196057E-5
110-111	0.12613083853360935	2.4369517220037824E-4	4.130426647464038E-5	3.717383982717634E-5	0.2278962902738283	6.195639971196057E-5
112-113	0.12614736024019918	2.4782559884784227E-4	4.3369479798372397E-5	3.717383982717634E-5	0.2284724847911495	6.195639971196057E-5
114-115	0.12615149066684664	2.4782559884784227E-4	4.5434693122104415E-5	3.717383982717634E-5	0.22908585314829794	6.195639971196057E-5
116-117	0.12616181673346533	2.4782559884784227E-4	4.5434693122104415E-5	3.717383982717634E-5	0.22969302586547513	6.195639971196057E-5
118-119	0.12617007758676024	2.4782559884784227E-4	4.5434693122104415E-5	3.717383982717634E-5	0.23037041583565926	6.195639971196057E-5
120-121	0.12617420801340767	2.4782559884784227E-4	4.5434693122104415E-5	3.717383982717634E-5	0.23100443632604498	6.402161303569259E-5
122-123	0.12618453408002636	2.4989081217157425E-4	4.956511976956845E-5	3.717383982717634E-5	0.23154345700353904	6.60868263594246E-5
124-125	0.12619692535996874	2.5402123881903833E-4	4.956511976956845E-5	3.717383982717634E-5	0.23215062972071626	7.021725300688864E-5
126-127	0.12620518621326368	2.5608645214277037E-4	4.956511976956845E-5	4.130426647464038E-5	0.23271236774477133	7.228246633062067E-5
128-129	0.12622170791985354	2.5608645214277037E-4	4.956511976956845E-5	4.130426647464038E-5	0.23343932283472502	7.434767965435268E-5
130-131	0.12622996877314846	2.5608645214277037E-4	4.956511976956845E-5	4.130426647464038E-5	0.23419519091121094	7.434767965435268E-5
132-133	0.12622996877314846	2.5608645214277037E-4	4.956511976956845E-5	4.130426647464038E-5	0.2348952982279561	7.847810630181672E-5
134-135	0.1262320339864722	2.643473054376984E-4	4.956511976956845E-5	4.130426647464038E-5	0.2355272535050181	7.847810630181672E-5
136-137	0.12623822962644338	2.746733720563585E-4	4.956511976956845E-5	4.130426647464038E-5	0.23616953484869876	7.847810630181672E-5
138-139	0.1262712730396231	2.788037987038226E-4	4.956511976956845E-5	4.130426647464038E-5	0.23679942491243702	7.847810630181672E-5
>>END_MODULE
