##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20058847
Total Bases	3 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.03326447427412	37.0	35.0	38.0	33.0	39.0
2	35.509376137122935	35.0	34.0	38.0	33.0	39.0
3	35.62122349305521	36.0	35.0	38.0	33.0	39.0
4	35.72386094774041	36.0	35.0	38.0	33.0	39.0
5	35.66053288107736	36.0	35.0	38.0	33.0	39.0
6	35.583255308742324	36.0	35.0	37.0	33.0	39.0
7	35.55720391107226	35.0	34.0	38.0	33.0	39.0
8	35.70250174399356	36.0	35.0	38.0	33.0	39.0
9	35.69906764830501	36.0	35.0	38.0	33.0	39.0
10-14	35.61625611880883	36.0	35.0	37.8	33.0	39.0
15-19	35.62487377265503	36.0	35.0	38.0	33.0	39.0
20-24	35.609556810518576	36.0	35.0	38.0	33.0	39.0
25-29	35.59739260187787	36.0	35.0	38.0	33.0	39.0
30-34	35.57428513214144	36.0	35.0	38.0	33.0	39.0
35-39	35.551713246529076	36.0	35.0	38.0	33.0	39.0
40-44	35.536551587436705	36.0	35.0	38.0	33.0	39.0
45-49	35.50490224089151	36.0	34.4	38.0	33.0	39.0
50-54	35.489545316338464	36.0	34.4	38.0	33.0	39.0
55-59	35.457795315952104	36.0	34.0	38.0	33.0	39.0
60-64	35.42177646601522	36.0	34.0	38.0	33.0	39.0
65-69	35.38954732542703	36.0	34.0	38.0	32.4	39.0
70-74	35.361388129636765	36.0	34.0	38.0	32.0	39.0
75-79	35.314350351244016	36.0	34.0	38.0	32.0	39.0
80-84	35.27618971319738	36.0	34.0	38.0	32.0	39.0
85-89	35.23522940276676	36.0	34.0	38.0	32.0	39.0
90-94	35.18235045115006	36.0	34.0	38.0	32.0	39.0
95-99	35.120085097613035	36.0	34.0	38.0	32.0	39.0
100-104	35.07116167743838	36.0	34.0	38.0	32.0	39.0
105-109	35.01009001165421	36.0	34.0	38.0	31.4	39.0
110-114	34.941832020554315	35.8	34.0	37.6	31.0	39.0
115-119	34.871745758866396	35.0	34.0	37.0	31.0	39.0
120-124	34.7966159869508	35.0	34.0	37.0	31.0	39.0
125-129	34.73848515819479	35.0	34.0	37.0	30.8	39.0
130-134	34.6469968388512	35.0	34.0	37.0	30.0	39.0
135-139	34.55942792723829	35.0	34.0	37.0	30.0	39.0
140-144	34.46607753675972	35.0	34.0	37.0	30.0	39.0
145-149	34.3769224522227	35.0	34.0	37.0	29.8	39.0
150	34.32403038918439	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	14.0
21	185.0
22	1594.0
23	8253.0
24	26869.0
25	63014.0
26	119448.0
27	195580.0
28	281177.0
29	361110.0
30	460663.0
31	597315.0
32	805466.0
33	1147205.0
34	1783134.0
35	3201359.0
36	9137609.0
37	1868849.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.43019666085493	19.264721446850857	15.75410092115464	31.55098097113957
2	24.818036649863277	25.804190041431596	32.06845837151058	17.309314937194547
3	20.178502782338388	29.464320656117472	30.05224078931356	20.30493577223058
4	23.69559046417725	34.038687968390605	21.91796576931694	20.347755798115205
5	25.803183004486748	35.12811578850968	21.76273641251663	17.305964794486943
6	19.291299210333435	39.620544472000034	21.80396619027834	19.284190127388186
7	20.65951746877575	21.21936520080142	36.996393661111235	21.124723669311603
8	21.624164140640787	23.925228603618145	28.132399633937084	26.318207621803985
9	23.791696501797936	23.545436086131968	27.957399545447455	24.705467866622644
10-14	23.178575606826417	29.401282622907416	25.21012644644294	22.21001532382323
15-19	23.26716086921646	28.121348151267117	26.720164922739574	21.89132605677684
20-24	23.184457212321327	28.150780550846218	26.66150252803663	22.003259708795824
25-29	23.344291922661355	27.747415392320406	26.619387445350174	22.288905239668065
30-34	23.181005368852954	27.458091683933777	26.84309322465045	22.517809722562816
35-39	23.46777183621543	27.180370109811097	26.851591488300315	22.500266565673158
40-44	23.622101745151173	26.820608650343747	26.96624812947871	22.591041475026373
45-49	23.53421909045919	26.643075746078527	27.08418783990924	22.738517323553044
50-54	23.681173882086735	26.63547444354476	26.872756289170376	22.81059538519813
55-59	23.78740114025497	26.57254327728807	26.910841884381494	22.72921369807547
60-64	23.52640607907324	26.519146389620502	26.993156685426634	22.961290845879624
65-69	23.678754815767824	26.503747697960904	26.897718497977475	22.919778988293793
70-74	23.89584529854428	26.425080428850972	26.6908165428533	22.988257729751442
75-79	23.743871220514322	26.568075423278316	26.88141347306752	22.80663988313984
80-84	24.016798173893044	26.545160845984817	26.593008062726636	22.845032917395503
85-89	23.866622044626993	26.63467845385131	26.723380461499108	22.77531904002259
90-94	23.850862414973303	26.46221490198315	26.723307675660518	22.963615007383027
95-99	23.858925123199263	26.684034831156637	26.756762844250986	22.700277201393114
100-104	24.075384034539812	26.423124184786133	26.737193087291537	22.76429869338251
105-109	23.883037223087396	26.610173089413824	26.700130405304563	22.80665928219422
110-114	23.976389071615134	26.720895772324297	26.592052873228454	22.710662282832107
115-119	24.074558223610758	26.73048754995738	26.738006426790133	22.456947799641725
120-124	23.979600622109537	26.804578548308385	26.95341561755768	22.2624052120244
125-129	24.015795364666726	26.8970437522859	26.763464787018563	22.32369609602881
130-134	24.175821272279507	26.89067921002638	26.704454149333706	22.229045368360406
135-139	23.983439327295333	26.9462865936412	26.91061455326919	22.15965952579428
140-144	23.991539493770507	27.069128150785538	26.78294719531985	22.15638516012411
145-149	24.115344216943278	27.704991219086523	26.222014655179333	21.95764990879087
150	23.952159363895642	27.62910051609646	26.194501608193132	22.22423851181476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	10.0
3	24.5
4	34.0
5	32.0
6	27.5
7	28.5
8	25.0
9	20.5
10	14.0
11	12.5
12	16.0
13	16.5
14	21.5
15	32.5
16	56.0
17	107.5
18	196.5
19	326.0
20	564.0
21	867.0
22	1285.5
23	2053.0
24	3396.0
25	5324.5
26	8075.0
27	12367.5
28	17959.5
29	24984.0
30	34982.0
31	49644.5
32	71833.0
33	97397.0
34	127612.0
35	177180.5
36	249642.5
37	333190.5
38	433520.5
39	561869.5
40	712943.5
41	871281.5
42	1030151.5
43	1179411.5
44	1289618.5
45	1351975.5
46	1367813.5
47	1328242.0
48	1266971.5
49	1209192.0
50	1111188.5
51	972327.5
52	826650.0
53	687032.0
54	561976.5
55	465190.5
56	383412.5
57	315144.5
58	264437.5
59	215085.0
60	161749.5
61	108894.5
62	67261.0
63	40950.5
64	25255.0
65	12994.0
66	7103.0
67	3807.0
68	1984.0
69	1233.0
70	854.0
71	686.5
72	424.0
73	302.0
74	250.5
75	160.0
76	99.5
77	33.0
78	7.5
79	1.5
80	1.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	4.985331410125417E-6
5	0.0
6	4.985331410125417E-6
7	0.0
8	0.0
9	0.0
10-14	0.09832369726933957
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	9.970662820250834E-7
40-44	9.970662820250834E-7
45-49	0.0
50-54	4.985331410125417E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	9.970662820250834E-7
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	5.982397692150501E-6
100-104	9.970662820250834E-7
105-109	1.994132564050167E-6
110-114	0.0
115-119	0.0
120-124	0.0
125-129	9.970662820250834E-7
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.0058847E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.7656767744916
#Duplication Level	Percentage of total
1	23.91484902954725
2	9.710612902365456
3	6.158518691536369
4	4.356905958151048
5	3.17138513447804
6	2.464608157664496
7	1.9915934424995225
8	1.7169815294795467
9	1.4564503487386062
>10	18.436740492533062
>50	6.437893604748466
>100	12.36042259106239
>500	3.288877120186194
>1k	3.8870462878225065
>5k	0.45163593698256865
>10k+	0.19547877220454116
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.5923723332652173E-4	4.985331410125417E-6	0.0	0.0	0.003355128039014406	0.0
2	0.0014008781262452423	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.013734588034895525	0.0
3	0.0021187658493033025	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.01848062353733492	0.0
4	0.005359231265884824	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.02633750583969258	0.0
5	0.005553659190879715	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.03123310128443574	0.0
6	0.0066354761068769304	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.04015185917715011	0.0
7	0.008868904578613118	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.05186738799094484	0.0
8	0.015290011434854655	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.07094126596608469	0.0
9	0.01577358858163682	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.08476060463495234	0.0
10-11	0.01640672567072275	1.994132564050167E-5	0.0	4.985331410125417E-6	0.14177783997255675	0.0
12-13	0.01714455471942131	2.4926657050627087E-5	0.0	4.985331410125417E-6	0.16973807118624518	0.0
14-15	0.019687073738585276	2.9911988460752504E-5	0.0	4.985331410125417E-6	0.1815508139625373	0.0
16-17	0.022658331259020024	2.9911988460752504E-5	0.0	4.985331410125417E-6	0.18973174280655314	0.0
18-19	0.028825186213345164	2.9911988460752504E-5	0.0	4.985331410125417E-6	0.19849346275984858	0.0
20-21	0.03411213017378317	2.9911988460752504E-5	0.0	4.985331410125417E-6	0.20430386651834972	4.985331410125417E-6
22-23	0.03619101337180547	4.9853314101254174E-5	0.0	4.985331410125417E-6	0.2102463815592192	4.985331410125417E-6
24-25	0.04077004027200566	5.483864551137959E-5	0.0	4.985331410125417E-6	0.2161764332715634	4.985331410125417E-6
26-27	0.04483308537125788	5.982397692150501E-5	0.0	4.985331410125417E-6	0.22095985875957874	4.985331410125417E-6
28-29	0.05440741434440374	6.231664262656771E-5	0.0	4.985331410125417E-6	0.2249929918703702	4.985331410125417E-6
30-31	0.07532835760699505	6.480930833163043E-5	0.0	4.985331410125417E-6	0.22951219479364893	4.985331410125417E-6
32-33	0.09360956788792496	7.477997115188127E-5	0.0	4.985331410125417E-6	0.2336824245182188	4.985331410125417E-6
34-35	0.10776043109556596	7.477997115188127E-5	0.0	4.985331410125417E-6	0.2372469364764585	4.985331410125417E-6
36-37	0.11062201132497795	7.477997115188127E-5	0.0	4.985331410125417E-6	0.23969722686453515	4.985331410125417E-6
38-39	0.11328417829798493	7.727263685694397E-5	0.0	4.985331410125417E-6	0.2417461980740967	4.985331410125417E-6
40-41	0.1149891616402478	7.976530256200668E-5	0.0	4.985331410125417E-6	0.24334648945674692	7.477997115188126E-6
42-43	0.11528578885915028	8.225796826706938E-5	0.0	4.985331410125417E-6	0.24496422949933264	9.970662820250835E-6
44-45	0.11602860323925897	8.47506339721321E-5	0.0	4.985331410125417E-6	0.24641496093967913	9.970662820250835E-6
46-47	0.1174569006882599	8.973596538225751E-5	0.0	4.985331410125417E-6	0.24786070704861551	9.970662820250835E-6
48-49	0.11872566753213681	9.222863108732023E-5	0.0	4.985331410125417E-6	0.24910205456973672	9.970662820250835E-6
50-51	0.11974516780550747	9.472129679238294E-5	0.0	4.985331410125417E-6	0.25044061605335544	9.970662820250835E-6
52-53	0.12075220475035281	9.472129679238294E-5	0.0	4.985331410125417E-6	0.25177668487126903	9.970662820250835E-6
54-55	0.12120586990867421	1.0469195961263377E-4	0.0	4.985331410125417E-6	0.2528834284443169	9.970662820250835E-6
56-57	0.12202097159422973	1.1964795384301002E-4	0.0	4.985331410125417E-6	0.25419955593659	9.970662820250835E-6
58-59	0.1221954581935841	1.2214061954807272E-4	0.0	4.985331410125417E-6	0.2553561528237391	9.970662820250835E-6
60-61	0.12242478343844987	1.2463328525313543E-4	0.0	7.477997115188126E-6	0.2562535124775617	9.970662820250835E-6
62-63	0.12259428470639414	1.2463328525313543E-4	0.0	9.970662820250835E-6	0.2573577633849044	9.970662820250835E-6
64-65	0.12261422603203465	1.3460394807338625E-4	0.0	9.970662820250835E-6	0.2586215448973712	9.970662820250835E-6
66-67	0.12267903534036627	1.445746108936371E-4	0.0	9.970662820250835E-6	0.25944910991145204	9.970662820250835E-6
68-69	0.12273387398587765	1.4955994230376253E-4	0.0	9.970662820250835E-6	0.26029163091976326	1.4955994230376252E-5
70-71	0.12285601460542572	1.4955994230376253E-4	0.0	1.2463328525313543E-5	0.261076820616858	1.4955994230376252E-5
72-73	0.12294076523939786	1.4955994230376253E-4	0.0	1.4955994230376252E-5	0.2617822450113907	2.2433991345564378E-5
74-75	0.12297067722785862	1.5703793941895065E-4	0.0	1.4955994230376252E-5	0.26269705332514875	2.4926657050627087E-5
76-77	0.1230055745477295	1.670086022392015E-4	0.0	1.4955994230376252E-5	0.2636243249674321	2.4926657050627087E-5
78-79	0.12305542786183074	1.695012679442642E-4	0.0	1.4955994230376252E-5	0.2647310685404799	2.7419322755689796E-5
80-81	0.12308783251599656	1.8445726217464044E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2656259355285975	2.9911988460752504E-5
82-83	0.12311774450445731	1.8944259358476587E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2664310665513327	2.9911988460752504E-5
84-85	0.12316759781855857	1.9193525928982858E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2673309188708603	2.9911988460752504E-5
86-87	0.12319252447560919	1.9442792499489128E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2682432345189133	2.9911988460752504E-5
88-89	0.12320748046983956	1.9442792499489128E-4	4.985331410125417E-6	1.4955994230376252E-5	0.26928267611792445	3.7389985575940634E-5
90-91	0.12322243646406994	2.043985878151421E-4	4.985331410125417E-6	1.4955994230376252E-5	0.26998560784675213	3.988265128100334E-5
92-93	0.12322243646406994	2.043985878151421E-4	4.985331410125417E-6	1.4955994230376252E-5	0.27061874493583804	4.2375316986066044E-5
94-95	0.12324237778971045	2.043985878151421E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2713141986675506	4.4867982691128756E-5
96-97	0.12324736312112057	2.1187658493033025E-4	4.985331410125417E-6	1.4955994230376252E-5	0.27199469640503265	5.483864551137959E-5
98-99	0.12324736312112057	2.1436925063539295E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2727848714335376	6.231664262656771E-5
100-101	0.12326481178105601	2.1436925063539295E-4	4.985331410125417E-6	1.4955994230376252E-5	0.27335818454570193	6.480930833163043E-5
102-103	0.12327727510958132	2.1935458204551836E-4	4.985331410125417E-6	2.2433991345564378E-5	0.27400378496331323	6.480930833163043E-5
104-105	0.12329472376951676	2.2433991345564377E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27472416535207633	6.480930833163043E-5
106-107	0.12331715776086233	2.293252448657692E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27523017649020404	6.480930833163043E-5
108-109	0.12334208441791294	2.3431057627589462E-4	4.985331410125417E-6	2.4926657050627087E-5	0.2757486509568571	6.979463974175584E-5
110-111	0.1233719964063737	2.3431057627589462E-4	4.985331410125417E-6	2.4926657050627087E-5	0.2763369200632519	6.979463974175584E-5
112-113	0.12339692306342434	2.3431057627589462E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27702738846355424	6.979463974175584E-5
114-115	0.12342434238618002	2.3431057627589462E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27769043754110095	6.979463974175584E-5
116-117	0.12343431304900027	2.3929590768602003E-4	4.985331410125417E-6	2.4926657050627087E-5	0.2782911699760211	7.228730544681856E-5
118-119	0.12344179104611547	2.4428123909614544E-4	4.985331410125417E-6	2.4926657050627087E-5	0.2789143364022867	7.477997115188127E-5
120-121	0.12344179104611547	2.4428123909614544E-4	4.985331410125417E-6	2.9911988460752504E-5	0.27958486347694855	7.727263685694397E-5
122-123	0.12345176170893571	2.4428123909614544E-4	4.985331410125417E-6	2.9911988460752504E-5	0.2802254785631497	7.976530256200668E-5
124-125	0.1234592397060509	2.467739048012082E-4	4.985331410125417E-6	2.9911988460752504E-5	0.2808935129721065	8.973596538225751E-5
126-127	0.12348416636310153	2.4926657050627085E-4	4.985331410125417E-6	2.9911988460752504E-5	0.2815839813724089	9.222863108732023E-5
128-129	0.1234866590288066	2.4926657050627085E-4	4.985331410125417E-6	3.489731987087792E-5	0.28239160506084926	1.0469195961263377E-4
130-131	0.12348915169451166	2.4926657050627085E-4	4.985331410125417E-6	3.489731987087792E-5	0.2832216627406351	1.0469195961263377E-4
132-133	0.12350410768874204	2.4926657050627085E-4	4.985331410125417E-6	3.489731987087792E-5	0.28389218981529696	1.0469195961263377E-4
134-135	0.12351158568585721	2.4926657050627085E-4	7.477997115188126E-6	3.489731987087792E-5	0.28453280490149807	1.0469195961263377E-4
136-137	0.12351158568585721	2.4926657050627085E-4	9.970662820250835E-6	3.489731987087792E-5	0.2851335373364182	1.0469195961263377E-4
138-139	0.12352903434579265	2.4926657050627085E-4	9.970662820250835E-6	3.489731987087792E-5	0.28573925510274845	1.0967729102275918E-4
>>END_MODULE
