##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.S.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24025985
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.02180522463491	37.0	35.0	38.0	33.0	39.0
2	35.52040251419453	35.0	34.0	38.0	33.0	39.0
3	35.62994137389164	36.0	35.0	37.0	33.0	39.0
4	35.724632933883875	36.0	35.0	38.0	33.0	39.0
5	35.66418200960335	36.0	35.0	38.0	33.0	39.0
6	35.59407637189484	36.0	35.0	37.0	33.0	39.0
7	35.56230647775731	35.0	34.0	38.0	33.0	39.0
8	35.70373135586325	36.0	35.0	38.0	33.0	39.0
9	35.69661664235618	36.0	35.0	38.0	33.0	39.0
10-14	35.616976302948665	36.0	35.0	37.8	33.0	39.0
15-19	35.62421722147916	36.0	35.0	38.0	33.0	39.0
20-24	35.60835790915544	36.0	35.0	38.0	33.0	39.0
25-29	35.59203092818047	36.0	35.0	38.0	33.0	39.0
30-34	35.56749674154879	36.0	35.0	38.0	33.0	39.0
35-39	35.54329054979432	36.0	35.0	38.0	33.0	39.0
40-44	35.52493968509511	36.0	35.0	38.0	33.0	39.0
45-49	35.49161228561493	36.0	34.2	38.0	33.0	39.0
50-54	35.475006606388874	36.0	34.0	38.0	33.0	39.0
55-59	35.43808662995502	36.0	34.0	38.0	33.0	39.0
60-64	35.400152792903185	36.0	34.0	38.0	32.8	39.0
65-69	35.363439217996685	36.0	34.0	38.0	32.0	39.0
70-74	35.331292032355805	36.0	34.0	38.0	32.0	39.0
75-79	35.280415383594054	36.0	34.0	38.0	32.0	39.0
80-84	35.23762337319364	36.0	34.0	38.0	32.0	39.0
85-89	35.19264007698331	36.0	34.0	38.0	32.0	39.0
90-94	35.13747668617957	36.0	34.0	38.0	32.0	39.0
95-99	35.06934692583884	36.0	34.0	37.8	32.0	39.0
100-104	35.014494240298575	36.0	34.0	37.6	31.6	39.0
105-109	34.94832430803565	35.6	34.0	37.0	31.0	39.0
110-114	34.873689990233494	35.0	34.0	37.0	31.0	39.0
115-119	34.79746823283209	35.0	34.0	37.0	31.0	39.0
120-124	34.71633121389196	35.0	34.0	37.0	30.8	39.0
125-129	34.6508156647896	35.0	34.0	37.0	30.0	39.0
130-134	34.553859431777724	35.0	34.0	37.0	30.0	39.0
135-139	34.45572920319397	35.0	34.0	37.0	30.0	39.0
140-144	34.35607254395605	35.0	34.0	37.0	29.8	39.0
145-149	34.25820761146734	35.0	34.0	37.0	29.0	39.0
150	34.192720464946596	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	20.0
21	229.0
22	1806.0
23	9289.0
24	30818.0
25	72249.0
26	138274.0
27	227105.0
28	327552.0
29	430969.0
30	559206.0
31	739384.0
32	1007347.0
33	1448406.0
34	2268229.0
35	4044524.0
36	1.0665562E7
37	2055014.0
38	1.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.08431264120651	19.79022126962386	15.573261480009434	31.5522046091602
2	24.847977064827326	26.167319709747535	31.567128583308566	17.417574642116577
3	20.24258734865605	29.630269060769	29.80278644142998	20.32435714914498
4	23.583658425810988	33.99940664240849	21.968681907055295	20.44825302472523
5	25.67533443477968	35.074066682385755	21.790540533509866	17.4600583493247
6	19.384820226933464	39.53751740043124	21.74471098687525	19.33295138576004
7	20.67108174753293	21.34348706202888	36.82713112490497	21.158300065533215
8	21.64671708568868	24.030577726573956	28.00114126434358	26.321563923393775
9	23.776660977687282	23.5330081160044	27.870303756536934	24.820027149771384
10-14	23.148832722929395	29.325124927957624	25.15203048068099	22.374011868431992
15-19	23.260391613496804	28.06733959086381	26.654957954897583	22.017310840741803
20-24	23.12424754385094	28.10566000191593	26.620315684208705	22.149776770024427
25-29	23.283956932462914	27.67982165975713	26.614309465355944	22.421911942424007
30-34	23.120299318594427	27.38993658232898	26.842192125252655	22.64757197382394
35-39	23.427972672088156	27.107310688822956	26.839322508525665	22.625394130563222
40-44	23.563886350549208	26.774884775795872	26.957452941055276	22.70377593259964
45-49	23.485458127818344	26.621445294512963	27.053782203341775	22.839314374326918
50-54	23.648636015929487	26.582927440841924	26.83833424948033	22.930102293748263
55-59	23.742661955378725	26.534069674978987	26.87910110657274	22.844167263069547
60-64	23.505712032249352	26.505361811433424	26.930078637192846	23.058847519124377
65-69	23.652571996527925	26.478086954603526	26.85984362347683	23.009497425391718
70-74	23.856943222098906	26.405321571623393	26.65643801908642	23.081297187191286
75-79	23.709073322071916	26.55461076829941	26.826004428122303	22.910311481506376
80-84	23.982452332339342	26.534390161319088	26.535265879838015	22.947891626503555
85-89	23.822001054275194	26.61579119440889	26.684131368599456	22.878076382716465
90-94	23.809508746467625	26.430441041230985	26.67194040119479	23.088109811106598
95-99	23.79188960724821	26.669850497523033	26.701741804264646	22.83651809096412
100-104	24.029437494691162	26.409665421914358	26.68912035605716	22.871776727337313
105-109	23.832270966890814	26.594886549666008	26.667617803537443	22.905224679905732
110-114	23.929172718031523	26.694631056288685	26.553619562766063	22.82257666291373
115-119	24.028778008477072	26.692751202500126	26.700009177563377	22.578461611459428
120-124	23.933999792308203	26.752545629242675	26.9186133263631	22.394841252086024
125-129	23.949437244716503	26.86563069110382	26.732216806095565	22.452715258084112
130-134	24.118529167482624	26.84965299029363	26.68905187446009	22.342765967763654
135-139	23.912805239826795	26.907418780124935	26.891312052346656	22.288463927701613
140-144	23.9270789522261	27.041229735222096	26.744977157023946	22.28671415552786
145-149	24.084607561354925	27.72893265354157	26.118643626889803	22.067816158213702
150	23.861444182205226	27.703692481286406	26.096657431526744	22.338205904981628
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	8.5
3	34.0
4	48.5
5	45.5
6	44.5
7	35.0
8	28.0
9	22.5
10	14.0
11	13.0
12	13.5
13	14.5
14	25.0
15	44.5
16	67.5
17	119.5
18	218.5
19	425.5
20	654.5
21	989.5
22	1527.5
23	2307.5
24	3721.0
25	5801.5
26	8903.0
27	13753.0
28	20143.5
29	28161.5
30	39670.0
31	56470.0
32	82861.0
33	113216.0
34	148064.0
35	205785.0
36	292421.0
37	391899.5
38	508297.5
39	660904.0
40	841292.5
41	1029528.5
42	1219255.0
43	1400608.0
44	1537510.5
45	1614863.5
46	1639274.0
47	1599337.5
48	1529331.0
49	1464776.0
50	1351474.5
51	1183050.5
52	1002512.0
53	830391.5
54	680983.5
55	564418.0
56	465883.0
57	383812.5
58	319208.0
59	257493.0
60	193843.5
61	131716.5
62	82610.5
63	50101.0
64	30360.5
65	15660.0
66	8614.5
67	4585.0
68	2417.5
69	1412.5
70	896.0
71	671.0
72	418.0
73	316.5
74	253.5
75	163.5
76	110.5
77	40.0
78	11.0
79	4.0
80	2.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.248648078320202E-5
2	2.0810801305336702E-5
3	0.0
4	4.16216026106734E-6
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.09902445206720974
15-19	0.0
20-24	8.324320522134681E-7
25-29	0.0
30-34	8.324320522134681E-7
35-39	0.0
40-44	0.0
45-49	8.324320522134681E-7
50-54	2.497296156640404E-6
55-59	0.0
60-64	8.324320522134681E-7
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	7.491888469921212E-6
100-104	8.324320522134681E-7
105-109	8.324320522134681E-7
110-114	8.324320522134681E-7
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4025985E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.30276822777664
#Duplication Level	Percentage of total
1	21.743737891132024
2	9.226097693232163
3	5.828097116574083
4	4.204567026164718
5	3.229410145289709
6	2.5077941630267193
7	2.121959944368722
8	1.8536602066945234
9	1.5050227966900176
>10	19.008498206283242
>50	6.556721755076994
>100	13.269746713963531
>500	3.7728904102193486
>1k	4.381327298998512
>5k	0.608023676053976
>10k+	0.18244495623167353
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.2881066062431986E-4	8.32432052213468E-6	0.0	0.0	0.0030675121124066297	0.0
2	0.001539999296594916	1.664864104426936E-5	0.0	0.0	0.012357453815108933	0.0
3	0.002397404310374788	1.664864104426936E-5	0.0	0.0	0.01683593825601739	0.0
4	0.0060809161414193845	2.0810801305336702E-5	0.0	0.0	0.02292517871795891	0.0
5	0.006309834955778088	2.0810801305336702E-5	0.0	0.0	0.027049879536676643	0.0
6	0.0074585911878326736	2.0810801305336702E-5	0.0	4.16216026106734E-6	0.03480814626330617	0.0
7	0.009647887485154095	2.497296156640404E-5	0.0	4.16216026106734E-6	0.04493052001822194	0.0
8	0.016407235749127454	2.497296156640404E-5	0.0	4.16216026106734E-6	0.06102559374776934	0.0
9	0.01710647867298677	2.497296156640404E-5	0.0	4.16216026106734E-6	0.07254229119014267	0.0
10-11	0.017745370273060605	2.497296156640404E-5	0.0	6.24324039160101E-6	0.12293772763114602	0.0
12-13	0.01844669427705045	3.1216201958005055E-5	0.0	8.32432052213468E-6	0.14955266142054113	0.0
14-15	0.020989774196562595	3.745944234960606E-5	0.0	8.32432052213468E-6	0.16148973704928227	0.0
16-17	0.023782583731738784	4.1621602610673404E-5	0.0	8.32432052213468E-6	0.16894624715698442	0.0
18-19	0.029515959491359045	4.786484300227441E-5	0.0	8.32432052213468E-6	0.1767960814093574	0.0
20-21	0.0347852543818703	4.994592313280808E-5	0.0	8.32432052213468E-6	0.18231718699566324	0.0
22-23	0.03711814520819854	4.994592313280808E-5	0.0	8.32432052213468E-6	0.18791737362692934	0.0
24-25	0.04172149445693902	5.827024365494276E-5	0.0	8.32432052213468E-6	0.19319083067770165	0.0
26-27	0.04563392510234232	6.24324039160101E-5	0.0	8.32432052213468E-6	0.19728847745472247	0.0
28-29	0.05430162384601506	6.24324039160101E-5	0.0	8.32432052213468E-6	0.20106771897177161	0.0
30-31	0.07393661487760023	6.659456417707744E-5	1.664864104426936E-5	8.32432052213468E-6	0.20521947383218628	0.0
32-33	0.09302636291498559	6.659456417707744E-5	1.664864104426936E-5	8.32432052213468E-6	0.20910485043589264	0.0
34-35	0.10824946406983939	6.867564430761112E-5	1.664864104426936E-5	1.248648078320202E-5	0.2125011732089236	0.0
36-37	0.11132321942263762	7.075672443814478E-5	1.664864104426936E-5	1.248648078320202E-5	0.21492771264112587	0.0
38-39	0.11411186679755272	7.699996482974579E-5	1.664864104426936E-5	1.248648078320202E-5	0.21698590089022365	0.0
40-41	0.1156601904146698	8.740536548241414E-5	1.664864104426936E-5	1.248648078320202E-5	0.21890049461031463	0.0
42-43	0.11593489299190023	9.156752574348149E-5	1.664864104426936E-5	1.248648078320202E-5	0.22069854784309573	0.0
44-45	0.11669656831967555	9.364860587401515E-5	1.664864104426936E-5	1.248648078320202E-5	0.2220783039696395	0.0
46-47	0.1180409460840003	9.989184626561616E-5	1.664864104426936E-5	1.248648078320202E-5	0.22350176277892458	0.0
48-49	0.11922091851801289	1.0405400652668351E-4	1.664864104426936E-5	1.248648078320202E-5	0.22474208653672262	2.08108013053367E-6
50-51	0.12008872893244543	1.1237832704881819E-4	1.664864104426936E-5	1.248648078320202E-5	0.22594078869191003	4.16216026106734E-6
52-53	0.12097735014818331	1.1445940717935186E-4	1.664864104426936E-5	1.248648078320202E-5	0.22718111244970812	4.16216026106734E-6
54-55	0.12142894453650913	1.2070264757095286E-4	1.664864104426936E-5	1.248648078320202E-5	0.22825911195732454	4.16216026106734E-6
56-57	0.12216564690271803	1.248648078320202E-4	1.664864104426936E-5	1.248648078320202E-5	0.22945989519264248	4.16216026106734E-6
58-59	0.12230716035159432	1.248648078320202E-4	1.872972117480303E-5	1.248648078320202E-5	0.23052748929960623	4.16216026106734E-6
60-61	0.1225652142877805	1.2694588796255385E-4	2.497296156640404E-5	1.248648078320202E-5	0.23140986727495252	4.16216026106734E-6
62-63	0.12276707906044226	1.2902696809308754E-4	2.497296156640404E-5	1.248648078320202E-5	0.23248162354217736	4.16216026106734E-6
64-65	0.12278372770148654	1.3735128861522224E-4	2.705404169693771E-5	1.456756091373569E-5	0.2335471365690106	4.16216026106734E-6
66-67	0.12285448442592467	1.4983776939842425E-4	2.913512182747138E-5	1.664864104426936E-5	0.23436708214044086	4.16216026106734E-6
68-69	0.12290026818879643	1.539999296594916E-4	2.913512182747138E-5	1.664864104426936E-5	0.23516413583043524	4.16216026106734E-6
70-71	0.1230230519164979	1.539999296594916E-4	2.913512182747138E-5	1.664864104426936E-5	0.2359341354787327	4.16216026106734E-6
72-73	0.12306259243897805	1.539999296594916E-4	2.913512182747138E-5	1.664864104426936E-5	0.23655429735763173	8.32432052213468E-6
74-75	0.12308340324028338	1.539999296594916E-4	2.913512182747138E-5	1.664864104426936E-5	0.237374242929062	1.248648078320202E-5
76-77	0.12311461944224139	1.664864104426936E-4	2.913512182747138E-5	2.0810801305336702E-5	0.23821291822166707	1.664864104426936E-5
78-79	0.1231770518461574	1.810539713564293E-4	2.913512182747138E-5	2.0810801305336702E-5	0.23929716096967513	1.872972117480303E-5
80-81	0.12321867344876808	1.8313505148696298E-4	3.329728208853872E-5	2.0810801305336702E-5	0.24007548493849473	2.497296156640404E-5
82-83	0.12323740316994287	1.8313505148696298E-4	3.329728208853872E-5	2.497296156640404E-5	0.24086421430796698	2.497296156640404E-5
84-85	0.12327278153216195	1.872972117480303E-4	3.329728208853872E-5	2.913512182747138E-5	0.24173826796279113	2.913512182747138E-5
86-87	0.12330191665398943	1.9145937200909765E-4	3.329728208853872E-5	2.913512182747138E-5	0.24258110541565725	3.1216201958005055E-5
88-89	0.12333521393607796	1.9145937200909765E-4	3.329728208853872E-5	2.913512182747138E-5	0.24359251035909663	3.329728208853872E-5
90-91	0.1233560247373833	1.9145937200909765E-4	3.329728208853872E-5	2.913512182747138E-5	0.24432505056504447	3.745944234960606E-5
92-93	0.12337059229829703	1.9145937200909765E-4	3.329728208853872E-5	2.913512182747138E-5	0.24498475296642364	3.745944234960606E-5
94-95	0.12340388958038558	1.9145937200909765E-4	3.745944234960606E-5	3.329728208853872E-5	0.24570064453132723	3.745944234960606E-5
96-97	0.12340388958038558	1.935404521396313E-4	3.745944234960606E-5	3.329728208853872E-5	0.2464352658174056	4.1621602610673404E-5
98-99	0.1234122139009077	2.0394585279229967E-4	3.745944234960606E-5	3.329728208853872E-5	0.2470783195777405	4.786484300227441E-5
100-101	0.1234247003816909	2.0394585279229967E-4	3.745944234960606E-5	3.329728208853872E-5	0.24770264361690064	5.618916352440909E-5
102-103	0.12343926794260464	2.0394585279229967E-4	4.1621602610673404E-5	3.329728208853872E-5	0.24830615685475538	5.827024365494276E-5
104-105	0.12347464630482372	2.0394585279229967E-4	4.5783762871740746E-5	3.329728208853872E-5	0.2489554538554819	5.827024365494276E-5
106-107	0.12348297062534586	2.0394585279229967E-4	4.5783762871740746E-5	3.537836221907239E-5	0.24953399413177024	6.24324039160101E-5
108-109	0.12351002466704278	2.0394585279229967E-4	4.5783762871740746E-5	3.745944234960606E-5	0.25020410193380205	6.24324039160101E-5
110-111	0.1235349976286092	2.0602693292283333E-4	4.5783762871740746E-5	3.745944234960606E-5	0.25083883137361485	6.24324039160101E-5
112-113	0.123553727349784	2.101890931839007E-4	5.410808339387542E-5	3.745944234960606E-5	0.25150685809551615	6.24324039160101E-5
114-115	0.1235787003113504	2.1227017331443436E-4	5.410808339387542E-5	3.745944234960606E-5	0.2521644794167648	6.659456417707744E-5
116-117	0.12359326787226413	2.1227017331443436E-4	5.410808339387542E-5	3.745944234960606E-5	0.25274301969305313	6.867564430761112E-5
118-119	0.12359534895239467	2.1643233357550168E-4	5.827024365494276E-5	3.745944234960606E-5	0.25332780320973314	7.699996482974579E-5
120-121	0.12359534895239467	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.25400207317202605	8.740536548241414E-5
122-123	0.12359743003252521	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.25461599181053346	8.740536548241414E-5
124-125	0.12360783543317787	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.25530691041387066	9.572968600454883E-5
126-127	0.12363280839474428	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.2559707749755109	9.572968600454883E-5
128-129	0.12363697055500535	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.2566242341364985	9.989184626561616E-5
130-131	0.12364529487552747	2.2059449383656903E-4	6.24324039160101E-5	4.1621602610673404E-5	0.2573318013808799	9.989184626561616E-5
132-133	0.12365570027618014	2.2475665409763638E-4	6.24324039160101E-5	4.1621602610673404E-5	0.2580227199842171	1.0197292639614983E-4
134-135	0.12366818675696334	2.2475665409763638E-4	7.075672443814478E-5	4.1621602610673404E-5	0.2586179089015497	1.0821616678775084E-4
136-137	0.12367859215761601	2.2683773422817004E-4	7.075672443814478E-5	4.1621602610673404E-5	0.25925471942149303	1.1237832704881819E-4
138-139	0.12368691647813815	2.289188143587037E-4	7.075672443814478E-5	4.370268274120707E-5	0.2599040164222195	1.186215674404192E-4
>>END_MODULE
