##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24210249
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.29647613289727	37.0	35.0	39.0	34.0	39.0
2	35.51000669179404	35.0	34.0	37.0	33.0	39.0
3	35.642248165229525	36.0	35.0	37.0	33.0	39.0
4	35.785194402585454	36.0	35.0	38.0	34.0	39.0
5	35.72744402587516	36.0	35.0	38.0	33.0	39.0
6	35.575123494186286	36.0	35.0	37.0	33.0	39.0
7	35.61207908270584	35.0	34.0	38.0	33.0	39.0
8	35.78566296447426	36.0	35.0	38.0	33.0	39.0
9	35.79050810258085	36.0	35.0	38.0	33.0	39.0
10-14	35.76025372560191	36.0	35.0	37.8	33.0	39.0
15-19	35.73027154739301	36.0	35.0	38.0	33.0	39.0
20-24	35.705206195937926	36.0	35.0	38.0	33.0	39.0
25-29	35.68705301626596	36.0	35.0	38.0	33.0	39.0
30-34	35.66659910850153	36.0	35.0	38.0	33.0	39.0
35-39	35.64404893976927	36.0	35.0	38.0	33.0	39.0
40-44	35.62365648531744	36.0	35.0	38.0	33.0	39.0
45-49	35.59425433418715	36.0	35.0	38.0	33.0	39.0
50-54	35.573453168532055	36.0	35.0	38.0	33.0	39.0
55-59	35.538763562489585	36.0	35.0	38.0	33.0	39.0
60-64	35.51322762520947	36.0	35.0	38.0	33.0	39.0
65-69	35.47554526184344	36.0	34.2	38.0	33.0	39.0
70-74	35.44769099235618	36.0	34.0	38.0	33.0	39.0
75-79	35.40563435758137	36.0	34.0	38.0	32.0	39.0
80-84	35.35390136631804	36.0	34.0	38.0	32.0	39.0
85-89	35.31794042266976	36.0	34.0	38.0	32.0	39.0
90-94	35.27541057508331	36.0	34.0	38.0	32.0	39.0
95-99	35.21503735876488	36.0	34.0	38.0	32.0	39.0
100-104	35.1614427179167	36.0	34.0	38.0	32.0	39.0
105-109	35.095726103436604	36.0	34.0	38.0	32.0	39.0
110-114	35.038601932594744	36.0	34.0	38.0	31.4	39.0
115-119	34.98058618893181	36.0	34.0	38.0	31.0	39.0
120-124	34.90580063839905	36.0	34.0	38.0	31.0	39.0
125-129	34.839582352085685	35.4	34.0	38.0	31.0	39.0
130-134	34.765611043488235	35.0	34.0	38.0	30.6	39.0
135-139	34.679865572634135	35.0	34.0	37.0	30.0	39.0
140-144	34.566829329182035	35.0	34.0	37.0	30.0	39.0
145-149	34.487926406704865	35.0	34.0	37.0	30.0	39.0
150	34.425290421424414	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	15.0
21	218.0
22	1761.0
23	7956.0
24	25894.0
25	61805.0
26	124502.0
27	217921.0
28	318815.0
29	414880.0
30	528300.0
31	687736.0
32	922872.0
33	1310551.0
34	2039623.0
35	3688357.0
36	1.1508044E7
37	2350991.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.493765223149914	19.980942781711995	11.751506562365385	38.773785432772705
2	24.28340575927162	24.612196264482865	37.38634823623664	13.718049740008869
3	16.732347527693747	29.92196817141369	31.434302885525877	21.91138141536669
4	21.020932085415563	34.74675952320854	23.550687149066498	20.681621242309404
5	25.13761423932484	35.3182034600305	22.984468272094187	16.55971402855047
6	17.883979631931915	38.62965226008208	24.3005885647851	19.185779543200898
7	19.098419846900377	20.526050764698866	39.7397317144487	20.63579767395205
8	19.20506063361843	22.282455665780223	31.32596860114904	27.1865150994523
9	22.417386124364107	22.416526984088435	30.76761416208483	24.398472729462632
10-14	22.869367431949996	29.039225494954636	26.025388669071514	22.06601840402385
15-19	22.900849140378522	28.40270085615394	27.13540286182104	21.5610471416465
20-24	23.039261267782408	28.36728235698721	27.03956473929676	21.553891635933624
25-29	23.03512202621295	28.50274939344903	27.01063751967194	21.451491060666083
30-34	23.161152121979413	28.229949225222757	27.027636105684	21.581262547113827
35-39	23.244467250212917	28.234719932042	27.051996862981458	21.46881595476362
40-44	23.41608878124302	27.945999233630353	27.16039888726465	21.477513097861983
45-49	23.446613043922017	27.816756448890718	27.19683469591742	21.539795811269848
50-54	23.556447519395608	27.683027960596355	27.196978436694312	21.563546083313724
55-59	23.68287661973241	27.662327636531124	27.065779455634676	21.589016288101785
60-64	23.65633744617827	27.626004176991326	27.09678450642949	21.62087387040092
65-69	23.746500913724596	27.502607676608363	27.087208396741396	21.66368301292564
70-74	23.767182237572197	27.488107206167108	27.000255965975402	21.744454590285294
75-79	23.749079986744455	27.551045014035168	27.042079575472357	21.657795423748016
80-84	23.8598666870279	27.581216290376336	26.947612565890257	21.611304456705508
85-89	23.866579181953718	27.498782251258813	26.98637363858896	21.648264928198508
90-94	23.79952639066207	27.452862628550413	27.070244506778927	21.67736647400859
95-99	23.932209238593202	27.57221478863943	26.998594909111382	21.496981063655983
100-104	24.044607918279635	27.616315946327656	26.829497101244165	21.509579034148548
105-109	23.846216534162867	27.74225081286855	26.885759002313442	21.525773650655143
110-114	23.8542354521013	27.714218056989004	26.875883019625284	21.555663471284415
115-119	23.953516545823216	27.743000908416924	26.83448898026617	21.468993565493687
120-124	23.925549051560765	27.654622635231878	27.034434879211695	21.385393433995663
125-129	23.91246203209228	27.690716439967222	27.023011617930905	21.3738099100096
130-134	23.95145067654158	27.63182177623883	27.093133384073276	21.32359416314631
135-139	23.883334698457663	27.659397472533225	27.167596665362677	21.289671163646435
140-144	23.93520033602298	27.86715576531245	27.124490954223564	21.07315294444101
145-149	24.064200335216555	28.367469664911006	26.68319368620629	20.885136313666145
150	23.73120573852834	28.325458362695898	26.782896780615516	21.160439118160244
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	3.0
3	6.5
4	8.5
5	10.0
6	13.0
7	13.0
8	11.0
9	10.0
10	7.5
11	7.5
12	18.0
13	27.5
14	41.0
15	68.5
16	117.0
17	206.0
18	341.0
19	642.5
20	1026.5
21	1422.0
22	2102.0
23	3340.0
24	5200.0
25	8510.0
26	13577.5
27	20596.5
28	32716.0
29	50113.0
30	65917.5
31	84081.5
32	109286.0
33	141731.0
34	190569.5
35	262247.0
36	360999.5
37	484838.0
38	634116.5
39	820132.5
40	1033541.0
41	1232952.0
42	1423396.0
43	1597802.5
44	1712039.5
45	1764123.0
46	1758503.5
47	1697677.5
48	1580767.5
49	1425249.0
50	1241031.0
51	1038730.0
52	844399.0
53	667635.5
54	517487.0
55	390674.5
56	289311.0
57	212651.5
58	152124.0
59	108199.0
60	74607.0
61	48830.0
62	32437.0
63	22494.5
64	15266.5
65	10076.5
66	7101.0
67	5313.5
68	4502.5
69	3143.5
70	1552.5
71	908.5
72	483.5
73	389.0
74	344.5
75	216.0
76	144.0
77	47.5
78	11.0
79	4.5
80	3.5
81	2.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.009812373263901582
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.01971313884462733
85-89	8.260964189174593E-7
90-94	0.0
95-99	8.260964189174593E-7
100-104	8.260964189174593E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	8.260964189174593E-7
135-139	0.0
140-144	0.0
145-149	0.009811547167482663
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4210249E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.77020349588073
#Duplication Level	Percentage of total
1	23.79404327420785
2	11.004635984490218
3	7.509943289104544
4	5.414905579562694
5	4.095079444201191
6	3.283834079295811
7	2.731027980973549
8	2.282881489712611
9	1.965778237002911
>10	22.470271190262224
>50	5.819481971223368
>100	7.047005789229077
>500	1.2448018205797384
>1k	1.1604000551356544
>5k	0.03125870649215537
>10k+	0.14465110852649368
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.130482094587296E-6	8.260964189174593E-6	0.0	0.0	7.310953307419515E-4	0.0
2	1.2391446283761889E-5	2.0652410472936482E-5	0.0	0.0	0.004659183802694471	0.0
3	4.1304820945872964E-5	2.4782892567523778E-5	0.0	4.130482094587296E-6	0.005985068555056992	0.0
4	7.434867770257133E-5	2.8913374662111075E-5	0.0	4.130482094587296E-6	0.0074885640374867685	0.0
5	7.847915979715864E-5	2.8913374662111075E-5	0.0	4.130482094587296E-6	0.009025103376673243	0.0
6	8.674012398633323E-5	2.8913374662111075E-5	0.0	4.130482094587296E-6	0.010925125140183399	0.0
7	9.087060608092052E-5	5.782674932422215E-5	0.0	4.130482094587296E-6	0.012891234617206952	0.0
8	1.3217542702679348E-4	5.782674932422215E-5	0.0	4.130482094587296E-6	0.01847151592699439	0.0
9	1.4456687331055538E-4	6.195723141880945E-5	0.0	4.130482094587296E-6	0.024609412319551112	0.0
10-11	1.5489307854702362E-4	6.195723141880945E-5	0.0	4.130482094587296E-6	0.030767961122580772	0.0
12-13	1.9619789949289657E-4	8.260964189174593E-5	0.0	8.260964189174593E-6	0.036536179367671937	0.0
14-15	2.189155510131267E-4	9.087060608092052E-5	0.0	8.260964189174593E-6	0.044607141380495506	0.0
16-17	2.313069972968886E-4	9.087060608092052E-5	4.130482094587296E-6	8.260964189174593E-6	0.049991224790790045	0.0
18-19	3.077209160467536E-4	9.087060608092052E-5	8.260964189174593E-6	8.260964189174593E-6	0.05723815562574346	0.0
20-21	3.510909780399202E-4	9.913157027009511E-5	8.260964189174593E-6	8.260964189174593E-6	0.06259539090242319	0.0
22-23	4.047872452695551E-4	1.197839807430316E-4	2.0652410472936482E-5	1.2391446283761889E-5	0.07024091325950427	0.0
24-25	4.543530304046026E-4	1.280449449322062E-4	2.0652410472936482E-5	1.2391446283761889E-5	0.0755960832951367	0.0
26-27	4.894621282085946E-4	1.3630590912138077E-4	2.0652410472936482E-5	1.2391446283761889E-5	0.0799537419049263	0.0
28-29	5.865284574313962E-4	1.3630590912138077E-4	2.0652410472936482E-5	1.2391446283761889E-5	0.08435477057670906	0.0
30-31	7.558782233094753E-4	1.3630590912138077E-4	2.271765152023013E-5	1.2391446283761889E-5	0.08919776083261266	0.0
32-33	8.942493734781497E-4	1.4456687331055538E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.09531293957364917	0.0
34-35	0.001016098595268475	1.4663211435784904E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.09873091350692015	0.0
36-37	0.001063599139356229	1.4869735540514267E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.10207866924458316	0.0
38-39	0.0011234911297277445	1.4869735540514267E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.10573414589829291	0.0
40-41	0.0011751221559100859	1.507625964524363E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.10860276571298379	2.065241047293648E-6
42-43	0.0011937093253357287	1.631540427361982E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.1112607309408507	4.130482094587296E-6
44-45	0.0012308836641870143	1.755454890199601E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.11363575814523841	4.130482094587296E-6
46-47	0.0012866451724639428	1.9826314054019023E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.11643829024641589	4.130482094587296E-6
48-49	0.0013547981270246334	2.0652410472936483E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.11889179661060074	4.130482094587296E-6
50-51	0.0014518644562474348	2.189155510131267E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.12084138415924595	4.130482094587296E-6
52-53	0.001538604580233768	2.2304603310771402E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.1227765150205601	4.130482094587296E-6
54-55	0.0016046922937471646	2.3750272043876954E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.12476740739015116	4.130482094587296E-6
56-57	0.0016831714535443234	2.436984435806505E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.12668808156413425	6.1957231418809446E-6
58-59	0.0017554548901996011	2.4576368462794415E-4	2.6848133614817426E-5	1.6521928378349185E-5	0.12849929796261078	8.260964189174593E-6
60-61	0.0018463254962805215	2.7674230033734886E-4	2.8913374662111075E-5	1.6521928378349185E-5	0.12988507470534483	8.260964189174593E-6
62-63	0.0018855650761791009	2.8913374662111076E-4	2.8913374662111075E-5	1.6521928378349185E-5	0.13168803013963218	8.260964189174593E-6
64-65	0.0019062174866520372	2.99459951857579E-4	2.8913374662111075E-5	1.6521928378349185E-5	0.13332370104908875	1.0326205236468241E-5
66-67	0.00203219719053695	3.2424284442510273E-4	3.0978615709404726E-5	1.6521928378349185E-5	0.13476523930009973	1.2391446283761889E-5
68-69	0.002061110565199061	3.614171832763885E-4	3.304385675669837E-5	1.6521928378349185E-5	0.13605808019570553	1.2391446283761889E-5
70-71	0.00207763249357741	3.6761290641826934E-4	3.304385675669837E-5	1.6521928378349185E-5	0.13743353073320314	1.2391446283761889E-5
72-73	0.0021148068324286957	3.75873870607444E-4	3.304385675669837E-5	1.6521928378349185E-5	0.138410389748573	1.2391446283761889E-5
74-75	0.002137524483948926	3.841348347966186E-4	3.304385675669837E-5	2.0652410472936482E-5	0.13955866377086829	1.4456687331055537E-5
76-77	0.0022056774385096163	4.192439326006106E-4	3.304385675669837E-5	2.0652410472936482E-5	0.14095683195988606	1.6521928378349185E-5
78-79	0.002284156598306775	4.4609206621542804E-4	3.304385675669837E-5	2.0652410472936482E-5	0.14274326546579508	2.0652410472936482E-5
80-81	0.002311004731921592	4.5228778935730896E-4	3.304385675669837E-5	2.0652410472936482E-5	0.144065019736063	2.0652410472936482E-5
82-83	0.002341983347630997	4.6467923564107085E-4	3.304385675669837E-5	2.271765152023013E-5	0.14583699655464097	2.0652410472936482E-5
84-85	0.0023894838917187513	4.6880971773565817E-4	3.304385675669837E-5	2.6848133614817426E-5	0.1473838620990639	2.4782892567523778E-5
86-87	0.0024266582305700365	4.7707068192483275E-4	3.717433885128567E-5	3.0978615709404726E-5	0.14921986139010796	2.4782892567523778E-5
88-89	0.0024473106410429733	4.8533164611400733E-4	3.717433885128567E-5	3.304385675669837E-5	0.1511942318313207	3.0978615709404726E-5
90-91	0.002459702087326735	5.039188155396501E-4	3.717433885128567E-5	3.304385675669837E-5	0.15224337428334586	3.304385675669837E-5
92-93	0.0024679630515159097	5.121797797288248E-4	3.717433885128567E-5	3.304385675669837E-5	0.15321197233452657	3.5109097803992016E-5
94-95	0.002482419738846965	5.245712260125866E-4	3.717433885128567E-5	3.304385675669837E-5	0.15430448484854492	3.717433885128567E-5
96-97	0.0024968764261780207	5.287017081071739E-4	3.717433885128567E-5	3.5109097803992016E-5	0.15518221229364473	3.717433885128567E-5
98-99	0.0025361160060766	5.307669491544676E-4	3.717433885128567E-5	3.717433885128567E-5	0.15603722208722431	3.717433885128567E-5
100-101	0.002546442211313068	5.348974312490549E-4	3.717433885128567E-5	3.717433885128567E-5	0.15681375272100673	4.337006199316661E-5
102-103	0.0025505726934076556	5.369626722963486E-4	4.1304820945872964E-5	3.717433885128567E-5	0.15771626305867403	4.9565785135047556E-5
104-105	0.0025691598628332984	5.410931543909359E-4	4.337006199316661E-5	4.1304820945872964E-5	0.1585712728522536	4.9565785135047556E-5
106-107	0.0026393780584412826	5.472888775328168E-4	4.543530304046026E-5	4.1304820945872964E-5	0.15944073933316422	4.9565785135047556E-5
108-109	0.002655899986819632	5.658760469584596E-4	4.543530304046026E-5	4.1304820945872964E-5	0.16024618334160876	4.9565785135047556E-5
110-111	0.00268894384357633	5.741370111476342E-4	4.543530304046026E-5	4.1304820945872964E-5	0.1611486936792761	5.369626722963485E-5
112-113	0.002695139566718211	5.906589395259835E-4	4.543530304046026E-5	4.1304820945872964E-5	0.16194381148248413	5.782674932422215E-5
114-115	0.0026972048077655046	5.947894216205707E-4	4.543530304046026E-5	4.543530304046026E-5	0.16274305976778677	5.98919903715158E-5
116-117	0.0027075310130019728	5.947894216205707E-4	4.9565785135047556E-5	4.750054408775391E-5	0.16356089522251505	6.40224724661031E-5
118-119	0.002719922459285735	5.98919903715158E-4	5.369626722963485E-5	4.9565785135047556E-5	0.16437253495410148	6.81529545606904E-5
120-121	0.0027261181824276156	5.98919903715158E-4	5.369626722963485E-5	4.9565785135047556E-5	0.1652172185424446	7.228343665527769E-5
122-123	0.002730248664522203	6.030503858097453E-4	5.369626722963485E-5	4.9565785135047556E-5	0.1659173352574771	7.434867770257133E-5
124-125	0.0027323139055694967	6.092461089516263E-4	5.5761508276928504E-5	5.16310261823412E-5	0.16670006161440143	7.434867770257133E-5
126-127	0.0027570967981370206	6.216375552353881E-4	5.782674932422215E-5	5.369626722963485E-5	0.16768724683500777	7.641391874986498E-5
128-129	0.0027860101727991316	6.360942425664436E-4	5.782674932422215E-5	5.369626722963485E-5	0.16862280102943178	7.847915979715864E-5
130-131	0.002806662583272068	6.443552067556183E-4	5.782674932422215E-5	5.369626722963485E-5	0.16971531354345015	8.054440084445228E-5
132-133	0.002829380234792298	6.443552067556183E-4	5.782674932422215E-5	5.369626722963485E-5	0.17064260677368498	8.260964189174593E-5
134-135	0.0028500326452652346	6.505509298974992E-4	5.782674932422215E-5	5.369626722963485E-5	0.17149348608516995	9.087060608092052E-5
136-137	0.0028582936094544093	6.526161709447929E-4	5.782674932422215E-5	5.369626722963485E-5	0.17247241034158714	9.293584712821417E-5
138-139	0.0028830765020219327	6.69138099323142E-4	5.782674932422215E-5	5.5761508276928504E-5	0.17329850676050462	9.500108817550782E-5
>>END_MODULE
