##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24234100
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.54594521768912	36.0	35.0	38.0	32.0	39.0
2	35.191849501322515	35.0	34.0	37.0	32.0	39.0
3	35.33582988433653	35.0	34.0	37.0	33.0	39.0
4	35.41298905261594	36.0	35.0	37.0	33.0	39.0
5	35.32487499019976	35.0	34.0	37.0	33.0	39.0
6	35.283481870587316	35.0	34.0	37.0	33.0	39.0
7	35.314713110864446	35.0	34.0	37.0	33.0	39.0
8	35.45707585592203	36.0	34.0	38.0	33.0	39.0
9	35.427953173420924	36.0	34.0	38.0	33.0	39.0
10-14	35.39258493610244	35.4	34.2	37.6	33.0	39.0
15-19	35.36591806586587	35.8	34.0	38.0	33.0	39.0
20-24	35.332695911958766	35.8	34.0	37.6	32.8	39.0
25-29	35.32178111008868	36.0	34.0	37.8	32.2	39.0
30-34	35.30168519565406	36.0	34.0	37.6	32.0	39.0
35-39	35.277445921243206	35.4	34.0	37.2	32.0	39.0
40-44	35.25576096492133	35.6	34.0	37.0	32.0	39.0
45-49	35.226975938863006	35.0	34.0	37.0	32.0	39.0
50-54	35.202051200581	35.0	34.0	37.0	32.0	39.0
55-59	35.17273360265081	35.0	34.0	37.0	32.0	39.0
60-64	35.13202495656946	35.0	34.0	37.0	32.0	39.0
65-69	35.0959169022163	35.0	34.0	37.0	32.0	39.0
70-74	35.0736495021478	35.0	34.0	37.0	32.0	39.0
75-79	35.02199392591431	35.0	34.0	37.0	32.0	39.0
80-84	34.9516119187426	35.0	34.0	37.0	31.6	39.0
85-89	34.93107540201616	35.0	34.0	37.0	31.0	39.0
90-94	34.878664716246945	35.0	34.0	37.0	31.0	39.0
95-99	34.816389038586124	35.0	34.0	37.0	31.0	39.0
100-104	34.75086051472924	35.0	34.0	37.0	31.0	39.0
105-109	34.6909791327097	35.0	34.0	37.0	30.8	39.0
110-114	34.626857403410895	35.0	34.0	37.0	30.0	39.0
115-119	34.56636432134884	35.0	34.0	37.0	30.0	39.0
120-124	34.49138721058343	35.0	34.0	37.0	30.0	39.0
125-129	34.411102661126264	35.0	34.0	37.0	30.0	39.0
130-134	34.33690922295443	35.0	34.0	37.0	30.0	39.0
135-139	34.24738104571657	35.0	34.0	37.0	29.0	39.0
140-144	34.14677351335515	35.0	34.0	37.0	29.0	39.0
145-149	34.042781254513265	35.0	34.0	37.0	28.8	39.0
150	33.972792222529414	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	331.0
22	2249.0
23	10711.0
24	34564.0
25	82536.0
26	164789.0
27	284329.0
28	416464.0
29	544833.0
30	694785.0
31	898876.0
32	1195543.0
33	1652645.0
34	2454421.0
35	4153037.0
36	1.0138435E7
37	1505539.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.13085280658246	18.2345001464878	16.334726686776072	32.29992036015366
2	24.159552036180422	24.76360995456815	34.20479819758109	16.872039811670334
3	19.531556773307035	28.986993533904702	30.98096896521843	20.50048072756983
4	23.353196528858096	33.89549436537771	22.469949368864533	20.281359736899656
5	25.316681865635616	35.04274967917109	22.541055785030185	17.099512670163115
6	18.700240570105763	38.86001130638233	23.011789173107317	19.427958950404594
7	20.066377542388615	20.10733223020455	38.73886795878535	21.087422268621488
8	21.291704664089032	22.83016080646692	28.73901650979405	27.13911801965
9	23.387338502358247	22.890773744434494	28.92184566375479	24.800042089452464
10-14	23.059946299305082	29.003664497577088	25.769749450317935	22.166639752799895
15-19	22.901494175562533	28.22136246033482	27.18473803442257	21.692405329680078
20-24	23.02706543075575	28.312775845996242	27.02749515948429	21.632663563763725
25-29	23.12365963662773	28.30322066839701	26.939071803780624	21.634047891194637
30-34	23.12512057906108	28.06699591127375	27.128519129066227	21.679364380598944
35-39	23.21181475689215	28.0505609863787	27.09399812660672	21.64362613012243
40-44	23.371746616311285	27.820228750564112	27.185806175478405	21.6222184576462
45-49	23.36663379287863	27.63802905822787	27.275671883833112	21.71966526506039
50-54	23.43637125733055	27.617062961835288	27.234577122604737	21.711988658229426
55-59	23.617959816952148	27.505454710511223	27.128166509175088	21.748418963361544
60-64	23.531486624219593	27.492040554425373	27.174256110191834	21.802216711163197
65-69	23.618422170564802	27.4169069816243	27.138398596509866	21.826272251301038
70-74	23.66823875174435	27.384994098274696	27.060226928668502	21.88654022131245
75-79	23.60523312192324	27.444063530314722	27.140249483166283	21.810453864595754
80-84	23.69903943108058	27.4894514255775	27.01745722823269	21.794051915109232
85-89	23.73515705118254	27.400886806621855	27.079748405424564	21.78420773677104
90-94	23.657816877870438	27.35181005277687	27.136784118246602	21.853588951106087
95-99	23.73832759407882	27.48237258641644	27.124923369342568	21.65437645016218
100-104	23.888946567027453	27.45621830396012	26.927914797743675	21.726920331268747
105-109	23.668055343503575	27.588065577017506	27.037489322896253	21.706389756582666
110-114	23.6854349862384	27.593338312543068	26.98400188164611	21.737224819572422
115-119	23.777166059395647	27.574148823352218	27.024708159164152	21.623976958087983
120-124	23.735410846699487	27.478293809136712	27.225409650038586	21.56088569412522
125-129	23.708024642961778	27.558533636487432	27.17917975084695	21.554261969703848
130-134	23.75847504136733	27.47476819853017	27.26056507153144	21.506191688571064
135-139	23.672262638183387	27.48945081517366	27.351507173775797	21.486779372867158
140-144	23.701999444600787	27.60762997270482	27.333504667666674	21.35686591502772
145-149	23.791424131693294	27.994510170732344	26.979063630174988	21.235002067399375
150	23.43397526625705	27.980085912000035	27.099331107819147	21.48660771392377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	3.5
2	11.0
3	21.0
4	30.5
5	33.5
6	23.5
7	13.0
8	9.5
9	10.5
10	10.5
11	8.0
12	12.5
13	18.0
14	25.0
15	40.5
16	76.0
17	131.5
18	226.0
19	413.0
20	628.0
21	938.5
22	1501.5
23	2473.5
24	4205.5
25	6995.5
26	10895.0
27	17125.0
28	28336.5
29	44041.0
30	59695.5
31	77348.5
32	101685.0
33	134133.5
34	181329.5
35	251146.5
36	348774.5
37	470889.0
38	616764.5
39	800066.0
40	1011828.5
41	1214437.0
42	1404725.0
43	1576978.5
44	1695169.0
45	1751861.5
46	1752628.0
47	1698031.0
48	1594073.0
49	1448735.5
50	1263121.5
51	1063029.5
52	871401.0
53	693630.0
54	542117.5
55	414362.5
56	310543.5
57	228724.0
58	164749.5
59	118524.5
60	82405.5
61	54627.0
62	36628.0
63	25315.5
64	17142.0
65	11206.0
66	7974.5
67	6056.5
68	5292.5
69	3828.5
70	1851.0
71	1102.0
72	589.5
73	424.5
74	376.0
75	261.0
76	180.0
77	65.0
78	14.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	8.252833816811849E-7
15-19	0.0
20-24	0.02774107559183135
25-29	0.0
30-34	8.252833816811849E-7
35-39	0.0
40-44	8.252833816811849E-7
45-49	0.0
50-54	8.252833816811849E-7
55-59	0.0
60-64	0.0
65-69	8.252833816811849E-7
70-74	8.252833816811849E-7
75-79	0.0
80-84	0.05328194568810064
85-89	1.6505667633623697E-6
90-94	0.0
95-99	8.252833816811849E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	8.252833816811849E-7
145-149	0.02906730598619301
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.42341E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.27563472123111
#Duplication Level	Percentage of total
1	28.960272235089125
2	10.623873301359108
3	6.811903730531483
4	5.19747323117344
5	3.9765030755149984
6	3.083925971882256
7	2.5659334190571226
8	2.157720404083035
9	1.9022800238268645
>10	21.208047709340555
>50	5.280012465972543
>100	6.197741348115153
>500	1.0013683531852122
>1k	0.8956541877757305
>5k	0.09045982962415407
>10k+	0.04683071346915101
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.252833816811847E-6	8.252833816811847E-6	0.0	0.0	0.0011347646498116291	0.0
2	2.4758501450435543E-5	1.6505667633623694E-5	0.0	4.1264169084059235E-6	0.005129136217148563	0.0
3	4.126416908405924E-5	1.6505667633623694E-5	0.0	4.1264169084059235E-6	0.006684795391617596	0.0
4	9.903400580174217E-5	2.4758501450435543E-5	0.0	4.1264169084059235E-6	0.008034133720666334	0.0
5	1.031604227101481E-4	3.301133526724739E-5	0.0	4.1264169084059235E-6	0.009692953317845514	0.0
6	1.031604227101481E-4	3.301133526724739E-5	0.0	4.1264169084059235E-6	0.011380657833383538	0.0
7	1.1553967343536587E-4	4.9517002900871086E-5	0.0	4.1264169084059235E-6	0.013629555048464767	0.0
8	1.6918309324464288E-4	4.9517002900871086E-5	0.0	4.1264169084059235E-6	0.019641744484012198	0.0
9	1.7743592706145471E-4	4.9517002900871086E-5	0.0	4.1264169084059235E-6	0.026074828444217032	0.0
10-11	2.1251047078290507E-4	5.1580211355074044E-5	0.0	4.1264169084059235E-6	0.03236142460417346	0.0
12-13	2.5790105677537026E-4	5.5706628263479976E-5	0.0	4.1264169084059235E-6	0.038385993290446105	0.0
14-15	3.0535485122203836E-4	5.7769836717682934E-5	0.0	4.1264169084059235E-6	0.0467399243215139	0.0
16-17	3.507454372145035E-4	6.189625362608886E-5	0.0	4.1264169084059235E-6	0.05268196466961843	0.0
18-19	4.2502094156581015E-4	7.221229589710366E-5	0.0	4.1264169084059235E-6	0.06036947936997866	0.0
20-21	4.7247473601247825E-4	7.633871280550959E-5	0.0	4.1264169084059235E-6	0.06634246784489624	0.0
22-23	5.42623823455379E-4	8.66547550765244E-5	4.1264169084059235E-6	4.1264169084059235E-6	0.07486351876075448	0.0
24-25	6.065832855356708E-4	8.871796353072736E-5	4.1264169084059235E-6	4.1264169084059235E-6	0.08027531453612885	2.0632084542029618E-6
26-27	6.416578292571212E-4	9.284438043913329E-5	4.1264169084059235E-6	4.1264169084059235E-6	0.08464312683367652	4.1264169084059235E-6
28-29	7.716399618719078E-4	9.903400580174218E-5	4.1264169084059235E-6	4.1264169084059235E-6	0.08913266843002216	4.1264169084059235E-6
30-31	9.655815565669862E-4	1.0728683961855402E-4	6.189625362608885E-6	4.1264169084059235E-6	0.09512628898948175	4.1264169084059235E-6
32-33	0.0011244486075406143	1.2172929879797475E-4	8.252833816811847E-6	4.1264169084059235E-6	0.1024609950441733	4.1264169084059235E-6
34-35	0.001244114697884386	1.2379250725217772E-4	8.252833816811847E-6	4.1264169084059235E-6	0.10591686920496327	4.1264169084059235E-6
36-37	0.001308074159964678	1.3204534106898955E-4	8.252833816811847E-6	6.189625362608885E-6	0.10930672069521871	4.1264169084059235E-6
38-39	0.0013740968304991727	1.4236138334000438E-4	8.252833816811847E-6	8.252833816811847E-6	0.11330315547100986	4.1264169084059235E-6
40-41	0.001417424208037435	1.4855100870261325E-4	8.252833816811847E-6	8.252833816811847E-6	0.11615038313780995	4.1264169084059235E-6
42-43	0.0014359930841252614	1.5886705097362808E-4	8.252833816811847E-6	8.252833816811847E-6	0.11915441464712946	4.1264169084059235E-6
44-45	0.0014690044193925089	1.7949913551565768E-4	8.252833816811847E-6	8.252833816811847E-6	0.12163851762598983	4.1264169084059235E-6
46-47	0.00151852142229338	2.1251047078290507E-4	8.252833816811847E-6	8.252833816811847E-6	0.12449606133506093	4.1264169084059235E-6
48-49	0.0015618487998316421	2.228265130539199E-4	8.252833816811847E-6	1.4442459179420732E-5	0.12691001522647838	4.1264169084059235E-6
50-51	0.0016196186365493252	2.3107934687073174E-4	8.252833816811847E-6	1.6505667633623694E-5	0.12899385576522338	4.1264169084059235E-6
52-53	0.001681514890175414	2.3107934687073174E-4	1.237925072521777E-5	1.6505667633623694E-5	0.13107563309551418	4.1264169084059235E-6
54-55	0.0017289686846220821	2.372689722333406E-4	1.6505667633623694E-5	1.6505667633623694E-5	0.13308313492145366	4.1264169084059235E-6
56-57	0.001790864938248171	2.3933218068754357E-4	1.8568876087826656E-5	1.6505667633623694E-5	0.13504524616140068	4.1264169084059235E-6
58-59	0.0018630772341452747	2.4758501450435543E-4	2.063208454202962E-5	1.8568876087826656E-5	0.13685055355882828	4.1264169084059235E-6
60-61	0.0019373527384965812	2.620274736837762E-4	2.063208454202962E-5	2.063208454202962E-5	0.13833400043740018	4.1264169084059235E-6
62-63	0.0019848065329432494	2.7853314131739986E-4	2.063208454202962E-5	2.063208454202962E-5	0.1404941796889507	4.1264169084059235E-6
64-65	0.002013691451302091	2.9503880895102353E-4	2.063208454202962E-5	2.063208454202962E-5	0.14214062003540467	4.1264169084059235E-6
66-67	0.002106535831741224	3.156708934930532E-4	2.063208454202962E-5	2.063208454202962E-5	0.14338473473328905	4.1264169084059235E-6
68-69	0.0021374839585542684	3.2805014421827093E-4	2.063208454202962E-5	2.063208454202962E-5	0.14469487210170792	4.1264169084059235E-6
70-71	0.002160179251550501	3.3217656112667686E-4	2.063208454202962E-5	2.063208454202962E-5	0.14603802080539408	4.1264169084059235E-6
72-73	0.0022035066290887633	3.5899827103131536E-4	2.063208454202962E-5	2.063208454202962E-5	0.146952022150606	4.1264169084059235E-6
74-75	0.002246834006627026	3.631246879397213E-4	2.063208454202962E-5	2.063208454202962E-5	0.14794442541707759	4.1264169084059235E-6
76-77	0.002275718924985867	3.878831893901569E-4	2.063208454202962E-5	2.063208454202962E-5	0.149341217540573	8.252833816811847E-6
78-79	0.0023293623447951444	4.126416908405924E-4	2.8884918358841467E-5	2.063208454202962E-5	0.15109907114355392	8.252833816811847E-6
80-81	0.002339678387066159	4.2502094156581015E-4	2.8884918358841467E-5	2.063208454202962E-5	0.15259902368975947	8.252833816811847E-6
82-83	0.002347931220882971	4.456530261078398E-4	3.507454372145035E-5	2.063208454202962E-5	0.15448685942535517	1.237925072521777E-5
84-85	0.002376816139241812	4.456530261078398E-4	3.713775217565331E-5	2.4758501450435543E-5	0.15599093838846914	1.6505667633623694E-5
86-87	0.002391258598421233	4.5184265147044865E-4	3.713775217565331E-5	2.8884918358841467E-5	0.1579159118762405	1.6505667633623694E-5
88-89	0.0024057010576006535	4.5803227683305754E-4	3.713775217565331E-5	2.8884918358841467E-5	0.15978724194420257	1.6505667633623694E-5
90-91	0.0024180803083258713	4.724747360124783E-4	3.713775217565331E-5	3.094812681304443E-5	0.160767265959949	1.6505667633623694E-5
92-93	0.002426333142142683	4.827907782834931E-4	4.126416908405924E-5	3.713775217565331E-5	0.16175347960105801	1.8568876087826656E-5
94-95	0.002434585975959495	4.951700290087109E-4	4.126416908405924E-5	3.713775217565331E-5	0.1627169979491708	2.063208454202962E-5
96-97	0.002436649184413698	4.992964459171168E-4	4.126416908405924E-5	3.713775217565331E-5	0.16358560870839026	2.269529299623258E-5
98-99	0.002440775601322104	5.116756966423346E-4	4.745379444666813E-5	4.126416908405924E-5	0.16450992609587317	2.4758501450435543E-5
100-101	0.0024449020182305096	5.178653220049435E-4	4.9517002900871086E-5	4.126416908405924E-5	0.16530013493383292	2.4758501450435543E-5
102-103	0.002446965226684713	5.281813642759582E-4	5.1580211355074044E-5	4.539058599246516E-5	0.16623270515513264	2.4758501450435543E-5
104-105	0.002457281268955728	5.323077811843641E-4	5.364341980927701E-5	4.539058599246516E-5	0.16719003387788284	3.094812681304443E-5
106-107	0.002488229395768772	5.570662826347997E-4	5.7769836717682934E-5	4.539058599246516E-5	0.1680813399300985	3.507454372145035E-5
108-109	0.002496482229585584	5.673823249058146E-4	6.189625362608886E-5	4.539058599246516E-5	0.16881584213979475	3.713775217565331E-5
110-111	0.002506798271856599	5.96267243264656E-4	6.189625362608886E-5	4.539058599246516E-5	0.16971127460891883	4.126416908405924E-5
112-113	0.0025129878972192077	6.230889531692945E-4	6.602267053449479E-5	4.539058599246516E-5	0.170505609863787	4.126416908405924E-5
114-115	0.002515051105673411	6.272153700777004E-4	7.427550435130663E-5	4.539058599246516E-5	0.17141342158363626	4.126416908405924E-5
116-117	0.0025212407310360196	6.292785785319034E-4	7.427550435130663E-5	4.539058599246516E-5	0.17229234838512675	4.126416908405924E-5
118-119	0.0025253671479444255	6.561002884365419E-4	7.427550435130663E-5	4.539058599246516E-5	0.1730908100569033	4.539058599246516E-5
120-121	0.0025253671479444255	6.622899137991508E-4	7.427550435130663E-5	4.539058599246516E-5	0.17394910477385173	5.364341980927701E-5
122-123	0.0025274303563986283	6.746691645243685E-4	7.427550435130663E-5	4.539058599246516E-5	0.17469598623427318	5.983304517188589E-5
124-125	0.0025398096071238465	6.829219983411804E-4	8.046512971391552E-5	4.539058599246516E-5	0.1755295224497712	6.808587898869774E-5
126-127	0.002562504900120079	6.849852067953834E-4	8.66547550765244E-5	4.539058599246516E-5	0.17653843138387643	7.633871280550959E-5
128-129	0.0025790105677537026	6.870484152495863E-4	8.871796353072736E-5	4.539058599246516E-5	0.17750607614889763	8.252833816811847E-5
130-131	0.0025893266100247172	7.159333336084278E-4	9.078117198493032E-5	4.539058599246516E-5	0.1784716577054646	8.252833816811847E-5
132-133	0.002609958694566747	7.241861674252396E-4	9.490758889333625E-5	4.539058599246516E-5	0.17934852129850087	8.252833816811847E-5
134-135	0.002638843612925588	7.283125843336455E-4	9.490758889333625E-5	4.9517002900871086E-5	0.18015936222100265	8.252833816811847E-5
136-137	0.0026470964467424	7.345022096962544E-4	9.697079734753922E-5	5.364341980927701E-5	0.18107955319157715	8.252833816811847E-5
138-139	0.002657412489013415	7.448182519672693E-4	1.031604227101481E-4	5.364341980927701E-5	0.18186357240417428	9.078117198493032E-5
>>END_MODULE
